Job ID = 9163532 sra ファイルのダウンロード中... Completed: 531641K bytes transferred in 9 seconds (466966K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 16314160 spots for /home/okishinya/chipatlas/results/sacCer3/SRX869395/SRR1793996.sra Written 16314160 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:52 16314160 reads; of these: 16314160 (100.00%) were unpaired; of these: 231109 (1.42%) aligned 0 times 14524804 (89.03%) aligned exactly 1 time 1558247 (9.55%) aligned >1 times 98.58% overall alignment rate Time searching: 00:02:52 Overall time: 00:02:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6191978 / 16083051 = 0.3850 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 10:58:30: # Command line: callpeak -t SRX869395.bam -f BAM -g 12100000 -n SRX869395.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX869395.20 # format = BAM # ChIP-seq file = ['SRX869395.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 10:58:30: # Command line: callpeak -t SRX869395.bam -f BAM -g 12100000 -n SRX869395.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX869395.10 # format = BAM # ChIP-seq file = ['SRX869395.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 10:58:30: #1 read tag files... INFO @ Wed, 28 Jun 2017 10:58:30: #1 read tag files... INFO @ Wed, 28 Jun 2017 10:58:30: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 10:58:30: # Command line: callpeak -t SRX869395.bam -f BAM -g 12100000 -n SRX869395.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX869395.05 # format = BAM # ChIP-seq file = ['SRX869395.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 10:58:30: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 10:58:30: #1 read tag files... INFO @ Wed, 28 Jun 2017 10:58:30: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 10:58:37: 1000000 INFO @ Wed, 28 Jun 2017 10:58:37: 1000000 INFO @ Wed, 28 Jun 2017 10:58:38: 1000000 INFO @ Wed, 28 Jun 2017 10:58:44: 2000000 INFO @ Wed, 28 Jun 2017 10:58:45: 2000000 INFO @ Wed, 28 Jun 2017 10:58:46: 2000000 INFO @ Wed, 28 Jun 2017 10:58:50: 3000000 INFO @ Wed, 28 Jun 2017 10:58:52: 3000000 INFO @ Wed, 28 Jun 2017 10:58:55: 3000000 INFO @ Wed, 28 Jun 2017 10:58:57: 4000000 INFO @ Wed, 28 Jun 2017 10:59:00: 4000000 INFO @ Wed, 28 Jun 2017 10:59:03: 4000000 INFO @ Wed, 28 Jun 2017 10:59:04: 5000000 INFO @ Wed, 28 Jun 2017 10:59:07: 5000000 INFO @ Wed, 28 Jun 2017 10:59:11: 6000000 INFO @ Wed, 28 Jun 2017 10:59:11: 5000000 INFO @ Wed, 28 Jun 2017 10:59:15: 6000000 INFO @ Wed, 28 Jun 2017 10:59:17: 7000000 INFO @ Wed, 28 Jun 2017 10:59:19: 6000000 INFO @ Wed, 28 Jun 2017 10:59:22: 7000000 INFO @ Wed, 28 Jun 2017 10:59:24: 8000000 INFO @ Wed, 28 Jun 2017 10:59:28: 7000000 INFO @ Wed, 28 Jun 2017 10:59:29: 8000000 INFO @ Wed, 28 Jun 2017 10:59:31: 9000000 INFO @ Wed, 28 Jun 2017 10:59:36: 8000000 INFO @ Wed, 28 Jun 2017 10:59:37: 9000000 INFO @ Wed, 28 Jun 2017 10:59:37: #1 tag size is determined as 49 bps INFO @ Wed, 28 Jun 2017 10:59:37: #1 tag size = 49 INFO @ Wed, 28 Jun 2017 10:59:37: #1 total tags in treatment: 9891073 INFO @ Wed, 28 Jun 2017 10:59:37: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 10:59:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 10:59:37: #1 tags after filtering in treatment: 9891073 INFO @ Wed, 28 Jun 2017 10:59:37: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 10:59:37: #1 finished! INFO @ Wed, 28 Jun 2017 10:59:37: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 10:59:37: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 10:59:38: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 10:59:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 10:59:38: Process for pairing-model is terminated! cat: SRX869395.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX869395.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX869395.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX869395.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 10:59:43: #1 tag size is determined as 49 bps INFO @ Wed, 28 Jun 2017 10:59:43: #1 tag size = 49 INFO @ Wed, 28 Jun 2017 10:59:43: #1 total tags in treatment: 9891073 INFO @ Wed, 28 Jun 2017 10:59:43: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 10:59:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 10:59:44: #1 tags after filtering in treatment: 9891073 INFO @ Wed, 28 Jun 2017 10:59:44: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 10:59:44: #1 finished! INFO @ Wed, 28 Jun 2017 10:59:44: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 10:59:44: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 10:59:44: 9000000 INFO @ Wed, 28 Jun 2017 10:59:44: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 10:59:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 10:59:44: Process for pairing-model is terminated! cat: SRX869395.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX869395.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX869395.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX869395.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 10:59:51: #1 tag size is determined as 49 bps INFO @ Wed, 28 Jun 2017 10:59:51: #1 tag size = 49 INFO @ Wed, 28 Jun 2017 10:59:51: #1 total tags in treatment: 9891073 INFO @ Wed, 28 Jun 2017 10:59:51: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 10:59:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 10:59:51: #1 tags after filtering in treatment: 9891073 INFO @ Wed, 28 Jun 2017 10:59:51: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 10:59:51: #1 finished! INFO @ Wed, 28 Jun 2017 10:59:51: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 10:59:51: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 10:59:51: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 10:59:51: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 10:59:51: Process for pairing-model is terminated! cat: SRX869395.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX869395.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX869395.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX869395.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。