Job ID = 10223981 SRX = SRX7636116 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 9821000 spots for SRR10970611/SRR10970611.sra Written 9821000 spots for SRR10970611/SRR10970611.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:12 9821000 reads; of these: 9821000 (100.00%) were unpaired; of these: 265062 (2.70%) aligned 0 times 8044610 (81.91%) aligned exactly 1 time 1511328 (15.39%) aligned >1 times 97.30% overall alignment rate Time searching: 00:01:13 Overall time: 00:01:13 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 5021929 / 9555938 = 0.5255 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 09:04:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7636116/SRX7636116.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7636116/SRX7636116.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7636116/SRX7636116.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7636116/SRX7636116.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 09:04:10: #1 read tag files... INFO @ Fri, 16 Oct 2020 09:04:10: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 09:04:15: 1000000 INFO @ Fri, 16 Oct 2020 09:04:21: 2000000 INFO @ Fri, 16 Oct 2020 09:04:27: 3000000 INFO @ Fri, 16 Oct 2020 09:04:33: 4000000 INFO @ Fri, 16 Oct 2020 09:04:37: #1 tag size is determined as 49 bps INFO @ Fri, 16 Oct 2020 09:04:37: #1 tag size = 49 INFO @ Fri, 16 Oct 2020 09:04:37: #1 total tags in treatment: 4534009 INFO @ Fri, 16 Oct 2020 09:04:37: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 09:04:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 09:04:37: #1 tags after filtering in treatment: 4534009 INFO @ Fri, 16 Oct 2020 09:04:37: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 16 Oct 2020 09:04:37: #1 finished! INFO @ Fri, 16 Oct 2020 09:04:37: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 09:04:37: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 09:04:37: #2 number of paired peaks: 0 WARNING @ Fri, 16 Oct 2020 09:04:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 09:04:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7636116/SRX7636116.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7636116/SRX7636116.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7636116/SRX7636116.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7636116/SRX7636116.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 09:04:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7636116/SRX7636116.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7636116/SRX7636116.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7636116/SRX7636116.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7636116/SRX7636116.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 09:04:40: #1 read tag files... INFO @ Fri, 16 Oct 2020 09:04:40: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 09:04:45: 1000000 INFO @ Fri, 16 Oct 2020 09:04:50: 2000000 INFO @ Fri, 16 Oct 2020 09:04:55: 3000000 INFO @ Fri, 16 Oct 2020 09:05:00: 4000000 INFO @ Fri, 16 Oct 2020 09:05:02: #1 tag size is determined as 49 bps INFO @ Fri, 16 Oct 2020 09:05:02: #1 tag size = 49 INFO @ Fri, 16 Oct 2020 09:05:02: #1 total tags in treatment: 4534009 INFO @ Fri, 16 Oct 2020 09:05:02: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 09:05:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 09:05:03: #1 tags after filtering in treatment: 4534009 INFO @ Fri, 16 Oct 2020 09:05:03: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 16 Oct 2020 09:05:03: #1 finished! INFO @ Fri, 16 Oct 2020 09:05:03: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 09:05:03: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 09:05:03: #2 number of paired peaks: 0 WARNING @ Fri, 16 Oct 2020 09:05:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 09:05:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7636116/SRX7636116.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7636116/SRX7636116.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7636116/SRX7636116.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7636116/SRX7636116.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 09:05:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7636116/SRX7636116.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7636116/SRX7636116.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7636116/SRX7636116.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7636116/SRX7636116.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 09:05:10: #1 read tag files... INFO @ Fri, 16 Oct 2020 09:05:10: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 09:05:14: 1000000 INFO @ Fri, 16 Oct 2020 09:05:20: 2000000 INFO @ Fri, 16 Oct 2020 09:05:25: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 16 Oct 2020 09:05:30: 4000000 INFO @ Fri, 16 Oct 2020 09:05:33: #1 tag size is determined as 49 bps INFO @ Fri, 16 Oct 2020 09:05:33: #1 tag size = 49 INFO @ Fri, 16 Oct 2020 09:05:33: #1 total tags in treatment: 4534009 INFO @ Fri, 16 Oct 2020 09:05:33: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 09:05:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 09:05:33: #1 tags after filtering in treatment: 4534009 INFO @ Fri, 16 Oct 2020 09:05:33: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 16 Oct 2020 09:05:33: #1 finished! INFO @ Fri, 16 Oct 2020 09:05:33: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 09:05:33: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 09:05:33: #2 number of paired peaks: 0 WARNING @ Fri, 16 Oct 2020 09:05:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 09:05:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7636116/SRX7636116.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7636116/SRX7636116.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7636116/SRX7636116.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7636116/SRX7636116.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。