Job ID = 10223980 SRX = SRX7636115 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 10477120 spots for SRR10970610/SRR10970610.sra Written 10477120 spots for SRR10970610/SRR10970610.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:16 10477120 reads; of these: 10477120 (100.00%) were unpaired; of these: 253509 (2.42%) aligned 0 times 8511741 (81.24%) aligned exactly 1 time 1711870 (16.34%) aligned >1 times 97.58% overall alignment rate Time searching: 00:01:16 Overall time: 00:01:16 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5505493 / 10223611 = 0.5385 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 09:04:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7636115/SRX7636115.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7636115/SRX7636115.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7636115/SRX7636115.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7636115/SRX7636115.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 09:04:11: #1 read tag files... INFO @ Fri, 16 Oct 2020 09:04:11: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 09:04:18: 1000000 INFO @ Fri, 16 Oct 2020 09:04:25: 2000000 INFO @ Fri, 16 Oct 2020 09:04:31: 3000000 INFO @ Fri, 16 Oct 2020 09:04:38: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 09:04:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7636115/SRX7636115.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7636115/SRX7636115.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7636115/SRX7636115.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7636115/SRX7636115.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 09:04:41: #1 read tag files... INFO @ Fri, 16 Oct 2020 09:04:41: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 09:04:43: #1 tag size is determined as 49 bps INFO @ Fri, 16 Oct 2020 09:04:43: #1 tag size = 49 INFO @ Fri, 16 Oct 2020 09:04:43: #1 total tags in treatment: 4718118 INFO @ Fri, 16 Oct 2020 09:04:43: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 09:04:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 09:04:43: #1 tags after filtering in treatment: 4718118 INFO @ Fri, 16 Oct 2020 09:04:43: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 16 Oct 2020 09:04:43: #1 finished! INFO @ Fri, 16 Oct 2020 09:04:43: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 09:04:43: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 09:04:43: #2 number of paired peaks: 0 WARNING @ Fri, 16 Oct 2020 09:04:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 09:04:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7636115/SRX7636115.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7636115/SRX7636115.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7636115/SRX7636115.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7636115/SRX7636115.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 16 Oct 2020 09:04:48: 1000000 INFO @ Fri, 16 Oct 2020 09:04:55: 2000000 INFO @ Fri, 16 Oct 2020 09:05:02: 3000000 INFO @ Fri, 16 Oct 2020 09:05:09: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 09:05:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7636115/SRX7636115.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7636115/SRX7636115.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7636115/SRX7636115.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7636115/SRX7636115.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 09:05:11: #1 read tag files... INFO @ Fri, 16 Oct 2020 09:05:11: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 09:05:14: #1 tag size is determined as 49 bps INFO @ Fri, 16 Oct 2020 09:05:14: #1 tag size = 49 INFO @ Fri, 16 Oct 2020 09:05:14: #1 total tags in treatment: 4718118 INFO @ Fri, 16 Oct 2020 09:05:14: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 09:05:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 09:05:14: #1 tags after filtering in treatment: 4718118 INFO @ Fri, 16 Oct 2020 09:05:14: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 16 Oct 2020 09:05:14: #1 finished! INFO @ Fri, 16 Oct 2020 09:05:14: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 09:05:14: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 09:05:14: #2 number of paired peaks: 0 WARNING @ Fri, 16 Oct 2020 09:05:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 09:05:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7636115/SRX7636115.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7636115/SRX7636115.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7636115/SRX7636115.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7636115/SRX7636115.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 16 Oct 2020 09:05:18: 1000000 INFO @ Fri, 16 Oct 2020 09:05:25: 2000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 16 Oct 2020 09:05:32: 3000000 BigWig に変換しました。 INFO @ Fri, 16 Oct 2020 09:05:38: 4000000 INFO @ Fri, 16 Oct 2020 09:05:43: #1 tag size is determined as 49 bps INFO @ Fri, 16 Oct 2020 09:05:43: #1 tag size = 49 INFO @ Fri, 16 Oct 2020 09:05:43: #1 total tags in treatment: 4718118 INFO @ Fri, 16 Oct 2020 09:05:43: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 09:05:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 09:05:43: #1 tags after filtering in treatment: 4718118 INFO @ Fri, 16 Oct 2020 09:05:43: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 16 Oct 2020 09:05:43: #1 finished! INFO @ Fri, 16 Oct 2020 09:05:43: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 09:05:43: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 09:05:43: #2 number of paired peaks: 0 WARNING @ Fri, 16 Oct 2020 09:05:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 09:05:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7636115/SRX7636115.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7636115/SRX7636115.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7636115/SRX7636115.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7636115/SRX7636115.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling