Job ID = 10223935 SRX = SRX7496386 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 10522215 spots for SRR10822997/SRR10822997.sra Written 10522215 spots for SRR10822997/SRR10822997.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:36 10522215 reads; of these: 10522215 (100.00%) were paired; of these: 5688152 (54.06%) aligned concordantly 0 times 4585629 (43.58%) aligned concordantly exactly 1 time 248434 (2.36%) aligned concordantly >1 times ---- 5688152 pairs aligned concordantly 0 times; of these: 55026 (0.97%) aligned discordantly 1 time ---- 5633126 pairs aligned 0 times concordantly or discordantly; of these: 11266252 mates make up the pairs; of these: 6323331 (56.13%) aligned 0 times 4652814 (41.30%) aligned exactly 1 time 290107 (2.58%) aligned >1 times 69.95% overall alignment rate Time searching: 00:03:36 Overall time: 00:03:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 197358 / 4888498 = 0.0404 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 08:59:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7496386/SRX7496386.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7496386/SRX7496386.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7496386/SRX7496386.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7496386/SRX7496386.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 08:59:51: #1 read tag files... INFO @ Fri, 16 Oct 2020 08:59:51: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 08:59:55: 1000000 INFO @ Fri, 16 Oct 2020 08:59:59: 2000000 INFO @ Fri, 16 Oct 2020 09:00:02: 3000000 INFO @ Fri, 16 Oct 2020 09:00:06: 4000000 INFO @ Fri, 16 Oct 2020 09:00:10: 5000000 INFO @ Fri, 16 Oct 2020 09:00:14: 6000000 INFO @ Fri, 16 Oct 2020 09:00:18: 7000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 09:00:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7496386/SRX7496386.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7496386/SRX7496386.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7496386/SRX7496386.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7496386/SRX7496386.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 09:00:21: #1 read tag files... INFO @ Fri, 16 Oct 2020 09:00:21: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 09:00:22: 8000000 INFO @ Fri, 16 Oct 2020 09:00:25: 1000000 INFO @ Fri, 16 Oct 2020 09:00:26: 9000000 INFO @ Fri, 16 Oct 2020 09:00:29: 2000000 INFO @ Fri, 16 Oct 2020 09:00:29: 10000000 INFO @ Fri, 16 Oct 2020 09:00:33: 3000000 INFO @ Fri, 16 Oct 2020 09:00:33: 11000000 INFO @ Fri, 16 Oct 2020 09:00:37: 4000000 INFO @ Fri, 16 Oct 2020 09:00:37: 12000000 INFO @ Fri, 16 Oct 2020 09:00:41: 5000000 INFO @ Fri, 16 Oct 2020 09:00:41: 13000000 INFO @ Fri, 16 Oct 2020 09:00:45: 6000000 INFO @ Fri, 16 Oct 2020 09:00:45: 14000000 INFO @ Fri, 16 Oct 2020 09:00:46: #1 tag size is determined as 37 bps INFO @ Fri, 16 Oct 2020 09:00:46: #1 tag size = 37 INFO @ Fri, 16 Oct 2020 09:00:46: #1 total tags in treatment: 4637123 INFO @ Fri, 16 Oct 2020 09:00:46: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 09:00:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 09:00:46: #1 tags after filtering in treatment: 2747554 INFO @ Fri, 16 Oct 2020 09:00:46: #1 Redundant rate of treatment: 0.41 INFO @ Fri, 16 Oct 2020 09:00:46: #1 finished! INFO @ Fri, 16 Oct 2020 09:00:46: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 09:00:46: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 09:00:47: #2 number of paired peaks: 0 WARNING @ Fri, 16 Oct 2020 09:00:47: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 09:00:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7496386/SRX7496386.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496386/SRX7496386.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496386/SRX7496386.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496386/SRX7496386.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 16 Oct 2020 09:00:48: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 09:00:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7496386/SRX7496386.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7496386/SRX7496386.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7496386/SRX7496386.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7496386/SRX7496386.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 09:00:51: #1 read tag files... INFO @ Fri, 16 Oct 2020 09:00:51: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 09:00:52: 8000000 INFO @ Fri, 16 Oct 2020 09:00:55: 1000000 INFO @ Fri, 16 Oct 2020 09:00:56: 9000000 INFO @ Fri, 16 Oct 2020 09:00:59: 2000000 INFO @ Fri, 16 Oct 2020 09:01:00: 10000000 INFO @ Fri, 16 Oct 2020 09:01:03: 3000000 INFO @ Fri, 16 Oct 2020 09:01:04: 11000000 INFO @ Fri, 16 Oct 2020 09:01:07: 4000000 INFO @ Fri, 16 Oct 2020 09:01:08: 12000000 INFO @ Fri, 16 Oct 2020 09:01:11: 5000000 INFO @ Fri, 16 Oct 2020 09:01:12: 13000000 INFO @ Fri, 16 Oct 2020 09:01:15: 6000000 INFO @ Fri, 16 Oct 2020 09:01:16: 14000000 INFO @ Fri, 16 Oct 2020 09:01:17: #1 tag size is determined as 37 bps INFO @ Fri, 16 Oct 2020 09:01:17: #1 tag size = 37 INFO @ Fri, 16 Oct 2020 09:01:17: #1 total tags in treatment: 4637123 INFO @ Fri, 16 Oct 2020 09:01:17: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 09:01:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 09:01:17: #1 tags after filtering in treatment: 2747554 INFO @ Fri, 16 Oct 2020 09:01:17: #1 Redundant rate of treatment: 0.41 INFO @ Fri, 16 Oct 2020 09:01:17: #1 finished! INFO @ Fri, 16 Oct 2020 09:01:17: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 09:01:17: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 09:01:18: #2 number of paired peaks: 0 WARNING @ Fri, 16 Oct 2020 09:01:18: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 09:01:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7496386/SRX7496386.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496386/SRX7496386.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496386/SRX7496386.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496386/SRX7496386.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 16 Oct 2020 09:01:19: 7000000 INFO @ Fri, 16 Oct 2020 09:01:23: 8000000 INFO @ Fri, 16 Oct 2020 09:01:27: 9000000 INFO @ Fri, 16 Oct 2020 09:01:31: 10000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 16 Oct 2020 09:01:35: 11000000 INFO @ Fri, 16 Oct 2020 09:01:39: 12000000 INFO @ Fri, 16 Oct 2020 09:01:43: 13000000 BigWig に変換しました。 INFO @ Fri, 16 Oct 2020 09:01:47: 14000000 INFO @ Fri, 16 Oct 2020 09:01:48: #1 tag size is determined as 37 bps INFO @ Fri, 16 Oct 2020 09:01:48: #1 tag size = 37 INFO @ Fri, 16 Oct 2020 09:01:48: #1 total tags in treatment: 4637123 INFO @ Fri, 16 Oct 2020 09:01:48: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 09:01:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 09:01:48: #1 tags after filtering in treatment: 2747554 INFO @ Fri, 16 Oct 2020 09:01:48: #1 Redundant rate of treatment: 0.41 INFO @ Fri, 16 Oct 2020 09:01:48: #1 finished! INFO @ Fri, 16 Oct 2020 09:01:48: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 09:01:48: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 09:01:48: #2 number of paired peaks: 0 WARNING @ Fri, 16 Oct 2020 09:01:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 09:01:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7496386/SRX7496386.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496386/SRX7496386.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496386/SRX7496386.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496386/SRX7496386.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling