Job ID = 10223933 SRX = SRX7496384 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 7319351 spots for SRR10822995/SRR10822995.sra Written 7319351 spots for SRR10822995/SRR10822995.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:41 7319351 reads; of these: 7319351 (100.00%) were paired; of these: 3663853 (50.06%) aligned concordantly 0 times 3466335 (47.36%) aligned concordantly exactly 1 time 189163 (2.58%) aligned concordantly >1 times ---- 3663853 pairs aligned concordantly 0 times; of these: 48121 (1.31%) aligned discordantly 1 time ---- 3615732 pairs aligned 0 times concordantly or discordantly; of these: 7231464 mates make up the pairs; of these: 3973160 (54.94%) aligned 0 times 3063469 (42.36%) aligned exactly 1 time 194835 (2.69%) aligned >1 times 72.86% overall alignment rate Time searching: 00:02:41 Overall time: 00:02:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 113734 / 3702548 = 0.0307 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 08:57:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7496384/SRX7496384.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7496384/SRX7496384.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7496384/SRX7496384.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7496384/SRX7496384.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 08:57:39: #1 read tag files... INFO @ Fri, 16 Oct 2020 08:57:39: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 08:57:43: 1000000 INFO @ Fri, 16 Oct 2020 08:57:46: 2000000 INFO @ Fri, 16 Oct 2020 08:57:50: 3000000 INFO @ Fri, 16 Oct 2020 08:57:54: 4000000 INFO @ Fri, 16 Oct 2020 08:57:58: 5000000 INFO @ Fri, 16 Oct 2020 08:58:01: 6000000 INFO @ Fri, 16 Oct 2020 08:58:05: 7000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 08:58:09: 8000000 INFO @ Fri, 16 Oct 2020 08:58:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7496384/SRX7496384.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7496384/SRX7496384.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7496384/SRX7496384.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7496384/SRX7496384.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 08:58:09: #1 read tag files... INFO @ Fri, 16 Oct 2020 08:58:09: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 08:58:12: 9000000 INFO @ Fri, 16 Oct 2020 08:58:13: 1000000 INFO @ Fri, 16 Oct 2020 08:58:16: 10000000 INFO @ Fri, 16 Oct 2020 08:58:16: 2000000 INFO @ Fri, 16 Oct 2020 08:58:18: #1 tag size is determined as 37 bps INFO @ Fri, 16 Oct 2020 08:58:18: #1 tag size = 37 INFO @ Fri, 16 Oct 2020 08:58:18: #1 total tags in treatment: 3542102 INFO @ Fri, 16 Oct 2020 08:58:18: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 08:58:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 08:58:18: #1 tags after filtering in treatment: 2293982 INFO @ Fri, 16 Oct 2020 08:58:18: #1 Redundant rate of treatment: 0.35 INFO @ Fri, 16 Oct 2020 08:58:18: #1 finished! INFO @ Fri, 16 Oct 2020 08:58:18: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 08:58:18: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 08:58:18: #2 number of paired peaks: 0 WARNING @ Fri, 16 Oct 2020 08:58:18: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 08:58:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7496384/SRX7496384.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496384/SRX7496384.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496384/SRX7496384.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496384/SRX7496384.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 16 Oct 2020 08:58:20: 3000000 INFO @ Fri, 16 Oct 2020 08:58:24: 4000000 INFO @ Fri, 16 Oct 2020 08:58:28: 5000000 INFO @ Fri, 16 Oct 2020 08:58:31: 6000000 INFO @ Fri, 16 Oct 2020 08:58:35: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 08:58:39: 8000000 INFO @ Fri, 16 Oct 2020 08:58:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX7496384/SRX7496384.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX7496384/SRX7496384.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX7496384/SRX7496384.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX7496384/SRX7496384.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 08:58:39: #1 read tag files... INFO @ Fri, 16 Oct 2020 08:58:39: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 08:58:43: 9000000 INFO @ Fri, 16 Oct 2020 08:58:43: 1000000 INFO @ Fri, 16 Oct 2020 08:58:46: 10000000 INFO @ Fri, 16 Oct 2020 08:58:48: 2000000 INFO @ Fri, 16 Oct 2020 08:58:48: #1 tag size is determined as 37 bps INFO @ Fri, 16 Oct 2020 08:58:48: #1 tag size = 37 INFO @ Fri, 16 Oct 2020 08:58:48: #1 total tags in treatment: 3542102 INFO @ Fri, 16 Oct 2020 08:58:48: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 08:58:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 08:58:48: #1 tags after filtering in treatment: 2293982 INFO @ Fri, 16 Oct 2020 08:58:48: #1 Redundant rate of treatment: 0.35 INFO @ Fri, 16 Oct 2020 08:58:48: #1 finished! INFO @ Fri, 16 Oct 2020 08:58:48: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 08:58:48: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 08:58:48: #2 number of paired peaks: 0 WARNING @ Fri, 16 Oct 2020 08:58:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 08:58:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7496384/SRX7496384.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496384/SRX7496384.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496384/SRX7496384.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496384/SRX7496384.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 16 Oct 2020 08:58:52: 3000000 INFO @ Fri, 16 Oct 2020 08:58:57: 4000000 INFO @ Fri, 16 Oct 2020 08:59:01: 5000000 INFO @ Fri, 16 Oct 2020 08:59:05: 6000000 INFO @ Fri, 16 Oct 2020 08:59:10: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 16 Oct 2020 08:59:14: 8000000 INFO @ Fri, 16 Oct 2020 08:59:19: 9000000 BigWig に変換しました。 INFO @ Fri, 16 Oct 2020 08:59:23: 10000000 INFO @ Fri, 16 Oct 2020 08:59:25: #1 tag size is determined as 37 bps INFO @ Fri, 16 Oct 2020 08:59:25: #1 tag size = 37 INFO @ Fri, 16 Oct 2020 08:59:25: #1 total tags in treatment: 3542102 INFO @ Fri, 16 Oct 2020 08:59:25: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 08:59:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 08:59:25: #1 tags after filtering in treatment: 2293982 INFO @ Fri, 16 Oct 2020 08:59:25: #1 Redundant rate of treatment: 0.35 INFO @ Fri, 16 Oct 2020 08:59:25: #1 finished! INFO @ Fri, 16 Oct 2020 08:59:25: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 08:59:25: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 08:59:25: #2 number of paired peaks: 0 WARNING @ Fri, 16 Oct 2020 08:59:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 08:59:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX7496384/SRX7496384.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496384/SRX7496384.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496384/SRX7496384.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX7496384/SRX7496384.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling