Job ID = 10223922 SRX = SRX4623312 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 13270802 spots for SRR7767717/SRR7767717.sra Written 13270802 spots for SRR7767717/SRR7767717.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:53 13270802 reads; of these: 13270802 (100.00%) were paired; of these: 1474591 (11.11%) aligned concordantly 0 times 10287965 (77.52%) aligned concordantly exactly 1 time 1508246 (11.37%) aligned concordantly >1 times ---- 1474591 pairs aligned concordantly 0 times; of these: 751027 (50.93%) aligned discordantly 1 time ---- 723564 pairs aligned 0 times concordantly or discordantly; of these: 1447128 mates make up the pairs; of these: 919754 (63.56%) aligned 0 times 245519 (16.97%) aligned exactly 1 time 281855 (19.48%) aligned >1 times 96.53% overall alignment rate Time searching: 00:04:53 Overall time: 00:04:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 137428 / 12502006 = 0.0110 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 08:56:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4623312/SRX4623312.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4623312/SRX4623312.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4623312/SRX4623312.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4623312/SRX4623312.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 08:56:48: #1 read tag files... INFO @ Fri, 16 Oct 2020 08:56:48: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 08:56:54: 1000000 INFO @ Fri, 16 Oct 2020 08:56:59: 2000000 INFO @ Fri, 16 Oct 2020 08:57:05: 3000000 INFO @ Fri, 16 Oct 2020 08:57:10: 4000000 INFO @ Fri, 16 Oct 2020 08:57:16: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 08:57:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4623312/SRX4623312.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4623312/SRX4623312.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4623312/SRX4623312.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4623312/SRX4623312.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 08:57:18: #1 read tag files... INFO @ Fri, 16 Oct 2020 08:57:18: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 08:57:22: 6000000 INFO @ Fri, 16 Oct 2020 08:57:24: 1000000 INFO @ Fri, 16 Oct 2020 08:57:28: 7000000 INFO @ Fri, 16 Oct 2020 08:57:30: 2000000 INFO @ Fri, 16 Oct 2020 08:57:34: 8000000 INFO @ Fri, 16 Oct 2020 08:57:36: 3000000 INFO @ Fri, 16 Oct 2020 08:57:40: 9000000 INFO @ Fri, 16 Oct 2020 08:57:42: 4000000 INFO @ Fri, 16 Oct 2020 08:57:46: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 08:57:48: 5000000 INFO @ Fri, 16 Oct 2020 08:57:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4623312/SRX4623312.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4623312/SRX4623312.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4623312/SRX4623312.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4623312/SRX4623312.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 08:57:48: #1 read tag files... INFO @ Fri, 16 Oct 2020 08:57:48: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 08:57:52: 11000000 INFO @ Fri, 16 Oct 2020 08:57:54: 1000000 INFO @ Fri, 16 Oct 2020 08:57:54: 6000000 INFO @ Fri, 16 Oct 2020 08:57:58: 12000000 INFO @ Fri, 16 Oct 2020 08:57:59: 2000000 INFO @ Fri, 16 Oct 2020 08:58:00: 7000000 INFO @ Fri, 16 Oct 2020 08:58:04: 3000000 INFO @ Fri, 16 Oct 2020 08:58:05: 13000000 INFO @ Fri, 16 Oct 2020 08:58:06: 8000000 INFO @ Fri, 16 Oct 2020 08:58:10: 4000000 INFO @ Fri, 16 Oct 2020 08:58:11: 14000000 INFO @ Fri, 16 Oct 2020 08:58:12: 9000000 INFO @ Fri, 16 Oct 2020 08:58:15: 5000000 INFO @ Fri, 16 Oct 2020 08:58:17: 15000000 INFO @ Fri, 16 Oct 2020 08:58:18: 10000000 INFO @ Fri, 16 Oct 2020 08:58:21: 6000000 INFO @ Fri, 16 Oct 2020 08:58:23: 16000000 INFO @ Fri, 16 Oct 2020 08:58:24: 11000000 INFO @ Fri, 16 Oct 2020 08:58:26: 7000000 INFO @ Fri, 16 Oct 2020 08:58:29: 17000000 INFO @ Fri, 16 Oct 2020 08:58:30: 12000000 INFO @ Fri, 16 Oct 2020 08:58:32: 8000000 INFO @ Fri, 16 Oct 2020 08:58:36: 18000000 INFO @ Fri, 16 Oct 2020 08:58:36: 13000000 INFO @ Fri, 16 Oct 2020 08:58:37: 9000000 INFO @ Fri, 16 Oct 2020 08:58:42: 14000000 INFO @ Fri, 16 Oct 2020 08:58:42: 19000000 INFO @ Fri, 16 Oct 2020 08:58:43: 10000000 INFO @ Fri, 16 Oct 2020 08:58:48: 15000000 INFO @ Fri, 16 Oct 2020 08:58:48: 20000000 INFO @ Fri, 16 Oct 2020 08:58:48: 11000000 INFO @ Fri, 16 Oct 2020 08:58:53: 16000000 INFO @ Fri, 16 Oct 2020 08:58:53: 12000000 INFO @ Fri, 16 Oct 2020 08:58:54: 21000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 16 Oct 2020 08:58:59: 13000000 INFO @ Fri, 16 Oct 2020 08:58:59: 17000000 INFO @ Fri, 16 Oct 2020 08:59:00: 22000000 INFO @ Fri, 16 Oct 2020 08:59:04: 14000000 INFO @ Fri, 16 Oct 2020 08:59:06: 18000000 INFO @ Fri, 16 Oct 2020 08:59:06: 23000000 BigWig に変換しました。 INFO @ Fri, 16 Oct 2020 08:59:10: 15000000 INFO @ Fri, 16 Oct 2020 08:59:11: 19000000 INFO @ Fri, 16 Oct 2020 08:59:13: 24000000 INFO @ Fri, 16 Oct 2020 08:59:15: 16000000 INFO @ Fri, 16 Oct 2020 08:59:17: 20000000 INFO @ Fri, 16 Oct 2020 08:59:19: 25000000 INFO @ Fri, 16 Oct 2020 08:59:21: #1 tag size is determined as 25 bps INFO @ Fri, 16 Oct 2020 08:59:21: #1 tag size = 25 INFO @ Fri, 16 Oct 2020 08:59:21: #1 total tags in treatment: 11660800 INFO @ Fri, 16 Oct 2020 08:59:21: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 08:59:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 08:59:21: 17000000 INFO @ Fri, 16 Oct 2020 08:59:21: #1 tags after filtering in treatment: 8509015 INFO @ Fri, 16 Oct 2020 08:59:21: #1 Redundant rate of treatment: 0.27 INFO @ Fri, 16 Oct 2020 08:59:21: #1 finished! INFO @ Fri, 16 Oct 2020 08:59:21: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 08:59:21: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 08:59:21: #2 number of paired peaks: 0 WARNING @ Fri, 16 Oct 2020 08:59:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 08:59:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4623312/SRX4623312.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623312/SRX4623312.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623312/SRX4623312.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623312/SRX4623312.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 16 Oct 2020 08:59:23: 21000000 INFO @ Fri, 16 Oct 2020 08:59:26: 18000000 INFO @ Fri, 16 Oct 2020 08:59:30: 22000000 INFO @ Fri, 16 Oct 2020 08:59:32: 19000000 INFO @ Fri, 16 Oct 2020 08:59:36: 23000000 INFO @ Fri, 16 Oct 2020 08:59:37: 20000000 INFO @ Fri, 16 Oct 2020 08:59:42: 24000000 INFO @ Fri, 16 Oct 2020 08:59:43: 21000000 INFO @ Fri, 16 Oct 2020 08:59:48: 25000000 INFO @ Fri, 16 Oct 2020 08:59:48: 22000000 INFO @ Fri, 16 Oct 2020 08:59:50: #1 tag size is determined as 25 bps INFO @ Fri, 16 Oct 2020 08:59:50: #1 tag size = 25 INFO @ Fri, 16 Oct 2020 08:59:50: #1 total tags in treatment: 11660800 INFO @ Fri, 16 Oct 2020 08:59:50: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 08:59:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 08:59:50: #1 tags after filtering in treatment: 8509015 INFO @ Fri, 16 Oct 2020 08:59:50: #1 Redundant rate of treatment: 0.27 INFO @ Fri, 16 Oct 2020 08:59:50: #1 finished! INFO @ Fri, 16 Oct 2020 08:59:50: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 08:59:50: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 08:59:51: #2 number of paired peaks: 0 WARNING @ Fri, 16 Oct 2020 08:59:51: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 08:59:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4623312/SRX4623312.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623312/SRX4623312.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623312/SRX4623312.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623312/SRX4623312.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 16 Oct 2020 08:59:54: 23000000 INFO @ Fri, 16 Oct 2020 08:59:59: 24000000 INFO @ Fri, 16 Oct 2020 09:00:04: 25000000 INFO @ Fri, 16 Oct 2020 09:00:05: #1 tag size is determined as 25 bps INFO @ Fri, 16 Oct 2020 09:00:05: #1 tag size = 25 INFO @ Fri, 16 Oct 2020 09:00:05: #1 total tags in treatment: 11660800 INFO @ Fri, 16 Oct 2020 09:00:05: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 09:00:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 09:00:06: #1 tags after filtering in treatment: 8509015 INFO @ Fri, 16 Oct 2020 09:00:06: #1 Redundant rate of treatment: 0.27 INFO @ Fri, 16 Oct 2020 09:00:06: #1 finished! INFO @ Fri, 16 Oct 2020 09:00:06: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 09:00:06: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 09:00:06: #2 number of paired peaks: 0 WARNING @ Fri, 16 Oct 2020 09:00:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Oct 2020 09:00:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4623312/SRX4623312.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623312/SRX4623312.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623312/SRX4623312.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4623312/SRX4623312.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling