Job ID = 2011045 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 24,315,223 reads read : 24,315,223 reads written : 24,315,223 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:28 24315223 reads; of these: 24315223 (100.00%) were unpaired; of these: 1517587 (6.24%) aligned 0 times 20232711 (83.21%) aligned exactly 1 time 2564925 (10.55%) aligned >1 times 93.76% overall alignment rate Time searching: 00:04:28 Overall time: 00:04:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 12228748 / 22797636 = 0.5364 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 02:09:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4553735/SRX4553735.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4553735/SRX4553735.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4553735/SRX4553735.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4553735/SRX4553735.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:09:25: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:09:25: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:09:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4553735/SRX4553735.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4553735/SRX4553735.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4553735/SRX4553735.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4553735/SRX4553735.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:09:26: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:09:26: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:09:33: 1000000 INFO @ Sat, 06 Jul 2019 02:09:35: 1000000 INFO @ Sat, 06 Jul 2019 02:09:41: 2000000 INFO @ Sat, 06 Jul 2019 02:09:45: 2000000 INFO @ Sat, 06 Jul 2019 02:09:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4553735/SRX4553735.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4553735/SRX4553735.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4553735/SRX4553735.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4553735/SRX4553735.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:09:46: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:09:46: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:09:48: 3000000 INFO @ Sat, 06 Jul 2019 02:09:54: 3000000 INFO @ Sat, 06 Jul 2019 02:09:55: 1000000 INFO @ Sat, 06 Jul 2019 02:09:57: 4000000 INFO @ Sat, 06 Jul 2019 02:10:03: 4000000 INFO @ Sat, 06 Jul 2019 02:10:05: 2000000 INFO @ Sat, 06 Jul 2019 02:10:07: 5000000 INFO @ Sat, 06 Jul 2019 02:10:13: 5000000 INFO @ Sat, 06 Jul 2019 02:10:14: 3000000 INFO @ Sat, 06 Jul 2019 02:10:16: 6000000 INFO @ Sat, 06 Jul 2019 02:10:22: 6000000 INFO @ Sat, 06 Jul 2019 02:10:23: 4000000 INFO @ Sat, 06 Jul 2019 02:10:25: 7000000 INFO @ Sat, 06 Jul 2019 02:10:32: 7000000 INFO @ Sat, 06 Jul 2019 02:10:32: 5000000 INFO @ Sat, 06 Jul 2019 02:10:34: 8000000 INFO @ Sat, 06 Jul 2019 02:10:41: 8000000 INFO @ Sat, 06 Jul 2019 02:10:42: 6000000 INFO @ Sat, 06 Jul 2019 02:10:44: 9000000 INFO @ Sat, 06 Jul 2019 02:10:50: 9000000 INFO @ Sat, 06 Jul 2019 02:10:51: 7000000 INFO @ Sat, 06 Jul 2019 02:10:53: 10000000 INFO @ Sat, 06 Jul 2019 02:10:58: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 02:10:58: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 02:10:58: #1 total tags in treatment: 10568888 INFO @ Sat, 06 Jul 2019 02:10:58: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:10:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:10:58: #1 tags after filtering in treatment: 10568888 INFO @ Sat, 06 Jul 2019 02:10:58: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:10:58: #1 finished! INFO @ Sat, 06 Jul 2019 02:10:58: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:10:58: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:10:59: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:10:59: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:10:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4553735/SRX4553735.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553735/SRX4553735.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553735/SRX4553735.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553735/SRX4553735.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 02:11:00: 10000000 INFO @ Sat, 06 Jul 2019 02:11:00: 8000000 INFO @ Sat, 06 Jul 2019 02:11:05: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 02:11:05: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 02:11:05: #1 total tags in treatment: 10568888 INFO @ Sat, 06 Jul 2019 02:11:05: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:11:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:11:05: #1 tags after filtering in treatment: 10568888 INFO @ Sat, 06 Jul 2019 02:11:05: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:11:05: #1 finished! INFO @ Sat, 06 Jul 2019 02:11:05: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:11:05: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:11:06: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:11:06: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:11:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4553735/SRX4553735.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553735/SRX4553735.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553735/SRX4553735.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553735/SRX4553735.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 02:11:08: 9000000 INFO @ Sat, 06 Jul 2019 02:11:16: 10000000 INFO @ Sat, 06 Jul 2019 02:11:21: #1 tag size is determined as 50 bps INFO @ Sat, 06 Jul 2019 02:11:21: #1 tag size = 50 INFO @ Sat, 06 Jul 2019 02:11:21: #1 total tags in treatment: 10568888 INFO @ Sat, 06 Jul 2019 02:11:21: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:11:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:11:21: #1 tags after filtering in treatment: 10568888 INFO @ Sat, 06 Jul 2019 02:11:21: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:11:21: #1 finished! INFO @ Sat, 06 Jul 2019 02:11:21: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:11:21: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:11:22: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:11:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:11:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4553735/SRX4553735.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553735/SRX4553735.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553735/SRX4553735.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4553735/SRX4553735.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。