Job ID = 2011003 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 36,849,098 reads read : 73,698,196 reads written : 36,849,098 reads 0-length : 36,849,098 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:37 36849098 reads; of these: 36849098 (100.00%) were unpaired; of these: 1004538 (2.73%) aligned 0 times 30672569 (83.24%) aligned exactly 1 time 5171991 (14.04%) aligned >1 times 97.27% overall alignment rate Time searching: 00:06:37 Overall time: 00:06:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 19317840 / 35844560 = 0.5389 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 02:01:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4409371/SRX4409371.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4409371/SRX4409371.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4409371/SRX4409371.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4409371/SRX4409371.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:01:47: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:01:47: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:01:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4409371/SRX4409371.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4409371/SRX4409371.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4409371/SRX4409371.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4409371/SRX4409371.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:01:48: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:01:48: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:01:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4409371/SRX4409371.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4409371/SRX4409371.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4409371/SRX4409371.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4409371/SRX4409371.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 02:01:49: #1 read tag files... INFO @ Sat, 06 Jul 2019 02:01:49: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 02:01:54: 1000000 INFO @ Sat, 06 Jul 2019 02:01:56: 1000000 INFO @ Sat, 06 Jul 2019 02:01:57: 1000000 INFO @ Sat, 06 Jul 2019 02:02:01: 2000000 INFO @ Sat, 06 Jul 2019 02:02:04: 2000000 INFO @ Sat, 06 Jul 2019 02:02:05: 2000000 INFO @ Sat, 06 Jul 2019 02:02:08: 3000000 INFO @ Sat, 06 Jul 2019 02:02:11: 3000000 INFO @ Sat, 06 Jul 2019 02:02:13: 3000000 INFO @ Sat, 06 Jul 2019 02:02:15: 4000000 INFO @ Sat, 06 Jul 2019 02:02:17: 4000000 INFO @ Sat, 06 Jul 2019 02:02:22: 5000000 INFO @ Sat, 06 Jul 2019 02:02:22: 4000000 INFO @ Sat, 06 Jul 2019 02:02:26: 5000000 INFO @ Sat, 06 Jul 2019 02:02:29: 6000000 INFO @ Sat, 06 Jul 2019 02:02:30: 5000000 INFO @ Sat, 06 Jul 2019 02:02:33: 6000000 INFO @ Sat, 06 Jul 2019 02:02:35: 7000000 INFO @ Sat, 06 Jul 2019 02:02:38: 6000000 INFO @ Sat, 06 Jul 2019 02:02:39: 7000000 INFO @ Sat, 06 Jul 2019 02:02:42: 8000000 INFO @ Sat, 06 Jul 2019 02:02:46: 8000000 INFO @ Sat, 06 Jul 2019 02:02:46: 7000000 INFO @ Sat, 06 Jul 2019 02:02:49: 9000000 INFO @ Sat, 06 Jul 2019 02:02:53: 9000000 INFO @ Sat, 06 Jul 2019 02:02:54: 8000000 INFO @ Sat, 06 Jul 2019 02:02:56: 10000000 INFO @ Sat, 06 Jul 2019 02:03:00: 10000000 INFO @ Sat, 06 Jul 2019 02:03:02: 9000000 INFO @ Sat, 06 Jul 2019 02:03:03: 11000000 INFO @ Sat, 06 Jul 2019 02:03:07: 11000000 INFO @ Sat, 06 Jul 2019 02:03:09: 12000000 INFO @ Sat, 06 Jul 2019 02:03:10: 10000000 INFO @ Sat, 06 Jul 2019 02:03:13: 12000000 INFO @ Sat, 06 Jul 2019 02:03:16: 13000000 INFO @ Sat, 06 Jul 2019 02:03:18: 11000000 INFO @ Sat, 06 Jul 2019 02:03:21: 13000000 INFO @ Sat, 06 Jul 2019 02:03:23: 14000000 INFO @ Sat, 06 Jul 2019 02:03:27: 12000000 INFO @ Sat, 06 Jul 2019 02:03:30: 14000000 INFO @ Sat, 06 Jul 2019 02:03:30: 15000000 INFO @ Sat, 06 Jul 2019 02:03:36: 13000000 INFO @ Sat, 06 Jul 2019 02:03:37: 16000000 INFO @ Sat, 06 Jul 2019 02:03:38: 15000000 INFO @ Sat, 06 Jul 2019 02:03:41: #1 tag size is determined as 51 bps INFO @ Sat, 06 Jul 2019 02:03:41: #1 tag size = 51 INFO @ Sat, 06 Jul 2019 02:03:41: #1 total tags in treatment: 16526720 INFO @ Sat, 06 Jul 2019 02:03:41: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:03:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:03:41: #1 tags after filtering in treatment: 16526720 INFO @ Sat, 06 Jul 2019 02:03:41: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:03:41: #1 finished! INFO @ Sat, 06 Jul 2019 02:03:41: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:03:41: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:03:42: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:03:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:03:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4409371/SRX4409371.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4409371/SRX4409371.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4409371/SRX4409371.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4409371/SRX4409371.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 06 Jul 2019 02:03:44: 14000000 INFO @ Sat, 06 Jul 2019 02:03:44: 16000000 INFO @ Sat, 06 Jul 2019 02:03:48: #1 tag size is determined as 51 bps INFO @ Sat, 06 Jul 2019 02:03:48: #1 tag size = 51 INFO @ Sat, 06 Jul 2019 02:03:48: #1 total tags in treatment: 16526720 INFO @ Sat, 06 Jul 2019 02:03:48: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:03:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:03:48: #1 tags after filtering in treatment: 16526720 INFO @ Sat, 06 Jul 2019 02:03:48: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:03:48: #1 finished! INFO @ Sat, 06 Jul 2019 02:03:48: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:03:48: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:03:50: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:03:50: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:03:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4409371/SRX4409371.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4409371/SRX4409371.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4409371/SRX4409371.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4409371/SRX4409371.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 02:03:52: 15000000 BigWig に変換しました。 INFO @ Sat, 06 Jul 2019 02:04:00: 16000000 INFO @ Sat, 06 Jul 2019 02:04:04: #1 tag size is determined as 51 bps INFO @ Sat, 06 Jul 2019 02:04:04: #1 tag size = 51 INFO @ Sat, 06 Jul 2019 02:04:04: #1 total tags in treatment: 16526720 INFO @ Sat, 06 Jul 2019 02:04:04: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 02:04:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 02:04:04: #1 tags after filtering in treatment: 16526720 INFO @ Sat, 06 Jul 2019 02:04:04: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 02:04:04: #1 finished! INFO @ Sat, 06 Jul 2019 02:04:04: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 02:04:04: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 02:04:05: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 02:04:05: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 02:04:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4409371/SRX4409371.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4409371/SRX4409371.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4409371/SRX4409371.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4409371/SRX4409371.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling