Job ID = 2010988 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 13,786,197 reads read : 27,572,394 reads written : 13,786,197 reads 0-length : 13,786,197 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:51 13786197 reads; of these: 13786197 (100.00%) were unpaired; of these: 2416233 (17.53%) aligned 0 times 10245488 (74.32%) aligned exactly 1 time 1124476 (8.16%) aligned >1 times 82.47% overall alignment rate Time searching: 00:02:51 Overall time: 00:02:51 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3982758 / 11369964 = 0.3503 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 06 Jul 2019 01:44:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4342636/SRX4342636.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4342636/SRX4342636.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4342636/SRX4342636.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4342636/SRX4342636.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:44:03: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:44:03: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:44:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4342636/SRX4342636.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4342636/SRX4342636.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4342636/SRX4342636.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4342636/SRX4342636.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:44:04: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:44:04: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:44:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX4342636/SRX4342636.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX4342636/SRX4342636.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX4342636/SRX4342636.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX4342636/SRX4342636.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 06 Jul 2019 01:44:05: #1 read tag files... INFO @ Sat, 06 Jul 2019 01:44:05: #1 read treatment tags... INFO @ Sat, 06 Jul 2019 01:44:12: 1000000 INFO @ Sat, 06 Jul 2019 01:44:17: 1000000 INFO @ Sat, 06 Jul 2019 01:44:18: 1000000 INFO @ Sat, 06 Jul 2019 01:44:21: 2000000 INFO @ Sat, 06 Jul 2019 01:44:29: 2000000 INFO @ Sat, 06 Jul 2019 01:44:30: 3000000 INFO @ Sat, 06 Jul 2019 01:44:30: 2000000 INFO @ Sat, 06 Jul 2019 01:44:38: 4000000 INFO @ Sat, 06 Jul 2019 01:44:40: 3000000 INFO @ Sat, 06 Jul 2019 01:44:42: 3000000 INFO @ Sat, 06 Jul 2019 01:44:47: 5000000 INFO @ Sat, 06 Jul 2019 01:44:52: 4000000 INFO @ Sat, 06 Jul 2019 01:44:54: 4000000 INFO @ Sat, 06 Jul 2019 01:44:56: 6000000 INFO @ Sat, 06 Jul 2019 01:45:03: 5000000 INFO @ Sat, 06 Jul 2019 01:45:04: 7000000 INFO @ Sat, 06 Jul 2019 01:45:05: 5000000 INFO @ Sat, 06 Jul 2019 01:45:07: #1 tag size is determined as 66 bps INFO @ Sat, 06 Jul 2019 01:45:07: #1 tag size = 66 INFO @ Sat, 06 Jul 2019 01:45:07: #1 total tags in treatment: 7387206 INFO @ Sat, 06 Jul 2019 01:45:07: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:45:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:45:08: #1 tags after filtering in treatment: 7387206 INFO @ Sat, 06 Jul 2019 01:45:08: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:45:08: #1 finished! INFO @ Sat, 06 Jul 2019 01:45:08: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:45:08: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:45:08: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:45:08: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:45:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4342636/SRX4342636.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342636/SRX4342636.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342636/SRX4342636.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342636/SRX4342636.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 06 Jul 2019 01:45:14: 6000000 INFO @ Sat, 06 Jul 2019 01:45:17: 6000000 INFO @ Sat, 06 Jul 2019 01:45:25: 7000000 INFO @ Sat, 06 Jul 2019 01:45:29: 7000000 INFO @ Sat, 06 Jul 2019 01:45:29: #1 tag size is determined as 66 bps INFO @ Sat, 06 Jul 2019 01:45:29: #1 tag size = 66 INFO @ Sat, 06 Jul 2019 01:45:29: #1 total tags in treatment: 7387206 INFO @ Sat, 06 Jul 2019 01:45:29: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:45:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:45:29: #1 tags after filtering in treatment: 7387206 INFO @ Sat, 06 Jul 2019 01:45:29: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:45:29: #1 finished! INFO @ Sat, 06 Jul 2019 01:45:29: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:45:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:45:30: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:45:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:45:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4342636/SRX4342636.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342636/SRX4342636.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342636/SRX4342636.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342636/SRX4342636.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 06 Jul 2019 01:45:33: #1 tag size is determined as 66 bps INFO @ Sat, 06 Jul 2019 01:45:33: #1 tag size = 66 INFO @ Sat, 06 Jul 2019 01:45:33: #1 total tags in treatment: 7387206 INFO @ Sat, 06 Jul 2019 01:45:33: #1 user defined the maximum tags... INFO @ Sat, 06 Jul 2019 01:45:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 06 Jul 2019 01:45:33: #1 tags after filtering in treatment: 7387206 INFO @ Sat, 06 Jul 2019 01:45:33: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 06 Jul 2019 01:45:33: #1 finished! INFO @ Sat, 06 Jul 2019 01:45:33: #2 Build Peak Model... INFO @ Sat, 06 Jul 2019 01:45:33: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 06 Jul 2019 01:45:33: #2 number of paired peaks: 0 WARNING @ Sat, 06 Jul 2019 01:45:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 06 Jul 2019 01:45:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX4342636/SRX4342636.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342636/SRX4342636.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342636/SRX4342636.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX4342636/SRX4342636.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。