Job ID = 2010334 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 26,381,530 reads read : 26,381,530 reads written : 26,381,530 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR1009216.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:54 26381530 reads; of these: 26381530 (100.00%) were unpaired; of these: 8346833 (31.64%) aligned 0 times 14696648 (55.71%) aligned exactly 1 time 3338049 (12.65%) aligned >1 times 68.36% overall alignment rate Time searching: 00:07:54 Overall time: 00:07:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 8928410 / 18034697 = 0.4951 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 22:24:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX363334/SRX363334.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX363334/SRX363334.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX363334/SRX363334.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX363334/SRX363334.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:24:15: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:24:15: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:24:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX363334/SRX363334.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX363334/SRX363334.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX363334/SRX363334.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX363334/SRX363334.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:24:16: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:24:16: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:24:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX363334/SRX363334.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX363334/SRX363334.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX363334/SRX363334.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX363334/SRX363334.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:24:17: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:24:17: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:24:28: 1000000 INFO @ Fri, 05 Jul 2019 22:24:28: 1000000 INFO @ Fri, 05 Jul 2019 22:24:31: 1000000 INFO @ Fri, 05 Jul 2019 22:24:39: 2000000 INFO @ Fri, 05 Jul 2019 22:24:40: 2000000 INFO @ Fri, 05 Jul 2019 22:24:44: 2000000 INFO @ Fri, 05 Jul 2019 22:24:50: 3000000 INFO @ Fri, 05 Jul 2019 22:24:51: 3000000 INFO @ Fri, 05 Jul 2019 22:24:58: 3000000 INFO @ Fri, 05 Jul 2019 22:25:02: 4000000 INFO @ Fri, 05 Jul 2019 22:25:04: 4000000 INFO @ Fri, 05 Jul 2019 22:25:11: 4000000 INFO @ Fri, 05 Jul 2019 22:25:12: 5000000 INFO @ Fri, 05 Jul 2019 22:25:15: 5000000 INFO @ Fri, 05 Jul 2019 22:25:21: 6000000 INFO @ Fri, 05 Jul 2019 22:25:26: 5000000 INFO @ Fri, 05 Jul 2019 22:25:26: 6000000 INFO @ Fri, 05 Jul 2019 22:25:31: 7000000 INFO @ Fri, 05 Jul 2019 22:25:37: 7000000 INFO @ Fri, 05 Jul 2019 22:25:40: 6000000 INFO @ Fri, 05 Jul 2019 22:25:41: 8000000 INFO @ Fri, 05 Jul 2019 22:25:48: 8000000 INFO @ Fri, 05 Jul 2019 22:25:50: 9000000 INFO @ Fri, 05 Jul 2019 22:25:51: #1 tag size is determined as 100 bps INFO @ Fri, 05 Jul 2019 22:25:51: #1 tag size = 100 INFO @ Fri, 05 Jul 2019 22:25:51: #1 total tags in treatment: 9106287 INFO @ Fri, 05 Jul 2019 22:25:51: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:25:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:25:52: #1 tags after filtering in treatment: 9106287 INFO @ Fri, 05 Jul 2019 22:25:52: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:25:52: #1 finished! INFO @ Fri, 05 Jul 2019 22:25:52: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:25:52: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:25:52: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 22:25:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:25:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX363334/SRX363334.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX363334/SRX363334.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX363334/SRX363334.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX363334/SRX363334.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 22:25:54: 7000000 INFO @ Fri, 05 Jul 2019 22:25:59: 9000000 INFO @ Fri, 05 Jul 2019 22:26:00: #1 tag size is determined as 100 bps INFO @ Fri, 05 Jul 2019 22:26:00: #1 tag size = 100 INFO @ Fri, 05 Jul 2019 22:26:00: #1 total tags in treatment: 9106287 INFO @ Fri, 05 Jul 2019 22:26:00: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:26:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:26:00: #1 tags after filtering in treatment: 9106287 INFO @ Fri, 05 Jul 2019 22:26:00: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:26:00: #1 finished! INFO @ Fri, 05 Jul 2019 22:26:00: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:26:00: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:26:00: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 22:26:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:26:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX363334/SRX363334.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 4 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX363334/SRX363334.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX363334/SRX363334.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX363334/SRX363334.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 22:26:07: 8000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 05 Jul 2019 22:26:19: 9000000 INFO @ Fri, 05 Jul 2019 22:26:20: #1 tag size is determined as 100 bps INFO @ Fri, 05 Jul 2019 22:26:20: #1 tag size = 100 INFO @ Fri, 05 Jul 2019 22:26:20: #1 total tags in treatment: 9106287 INFO @ Fri, 05 Jul 2019 22:26:20: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:26:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:26:21: #1 tags after filtering in treatment: 9106287 INFO @ Fri, 05 Jul 2019 22:26:21: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:26:21: #1 finished! INFO @ Fri, 05 Jul 2019 22:26:21: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:26:21: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:26:21: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 22:26:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:26:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX363334/SRX363334.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX363334/SRX363334.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX363334/SRX363334.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX363334/SRX363334.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。