Job ID = 5790845 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 14,579,922 reads read : 14,579,922 reads written : 14,579,922 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR6055814.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:50 14579922 reads; of these: 14579922 (100.00%) were unpaired; of these: 769428 (5.28%) aligned 0 times 9876827 (67.74%) aligned exactly 1 time 3933667 (26.98%) aligned >1 times 94.72% overall alignment rate Time searching: 00:01:50 Overall time: 00:01:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6941323 / 13810494 = 0.5026 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 07:55:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3202743/SRX3202743.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3202743/SRX3202743.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3202743/SRX3202743.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3202743/SRX3202743.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 07:55:01: #1 read tag files... INFO @ Wed, 22 Apr 2020 07:55:01: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 07:55:07: 1000000 INFO @ Wed, 22 Apr 2020 07:55:12: 2000000 INFO @ Wed, 22 Apr 2020 07:55:17: 3000000 INFO @ Wed, 22 Apr 2020 07:55:22: 4000000 INFO @ Wed, 22 Apr 2020 07:55:27: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 07:55:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3202743/SRX3202743.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3202743/SRX3202743.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3202743/SRX3202743.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3202743/SRX3202743.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 07:55:31: #1 read tag files... INFO @ Wed, 22 Apr 2020 07:55:31: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 07:55:33: 6000000 INFO @ Wed, 22 Apr 2020 07:55:37: 1000000 INFO @ Wed, 22 Apr 2020 07:55:38: #1 tag size is determined as 51 bps INFO @ Wed, 22 Apr 2020 07:55:38: #1 tag size = 51 INFO @ Wed, 22 Apr 2020 07:55:38: #1 total tags in treatment: 6869171 INFO @ Wed, 22 Apr 2020 07:55:38: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 07:55:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 07:55:38: #1 tags after filtering in treatment: 6869171 INFO @ Wed, 22 Apr 2020 07:55:38: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 07:55:38: #1 finished! INFO @ Wed, 22 Apr 2020 07:55:38: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 07:55:38: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 07:55:38: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 07:55:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 07:55:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3202743/SRX3202743.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3202743/SRX3202743.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3202743/SRX3202743.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3202743/SRX3202743.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 07:55:43: 2000000 INFO @ Wed, 22 Apr 2020 07:55:49: 3000000 INFO @ Wed, 22 Apr 2020 07:55:54: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 07:55:59: 5000000 INFO @ Wed, 22 Apr 2020 07:56:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3202743/SRX3202743.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3202743/SRX3202743.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3202743/SRX3202743.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3202743/SRX3202743.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 07:56:01: #1 read tag files... INFO @ Wed, 22 Apr 2020 07:56:01: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 07:56:05: 6000000 INFO @ Wed, 22 Apr 2020 07:56:08: 1000000 INFO @ Wed, 22 Apr 2020 07:56:10: #1 tag size is determined as 51 bps INFO @ Wed, 22 Apr 2020 07:56:10: #1 tag size = 51 INFO @ Wed, 22 Apr 2020 07:56:10: #1 total tags in treatment: 6869171 INFO @ Wed, 22 Apr 2020 07:56:10: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 07:56:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 07:56:10: #1 tags after filtering in treatment: 6869171 INFO @ Wed, 22 Apr 2020 07:56:10: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 07:56:10: #1 finished! INFO @ Wed, 22 Apr 2020 07:56:10: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 07:56:10: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 07:56:10: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 07:56:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 07:56:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3202743/SRX3202743.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3202743/SRX3202743.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3202743/SRX3202743.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3202743/SRX3202743.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 07:56:14: 2000000 INFO @ Wed, 22 Apr 2020 07:56:21: 3000000 INFO @ Wed, 22 Apr 2020 07:56:27: 4000000 INFO @ Wed, 22 Apr 2020 07:56:34: 5000000 INFO @ Wed, 22 Apr 2020 07:56:40: 6000000 INFO @ Wed, 22 Apr 2020 07:56:46: #1 tag size is determined as 51 bps INFO @ Wed, 22 Apr 2020 07:56:46: #1 tag size = 51 INFO @ Wed, 22 Apr 2020 07:56:46: #1 total tags in treatment: 6869171 INFO @ Wed, 22 Apr 2020 07:56:46: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 07:56:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 07:56:46: #1 tags after filtering in treatment: 6869171 INFO @ Wed, 22 Apr 2020 07:56:46: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 07:56:46: #1 finished! INFO @ Wed, 22 Apr 2020 07:56:46: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 07:56:46: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 07:56:46: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 07:56:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 07:56:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3202743/SRX3202743.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3202743/SRX3202743.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3202743/SRX3202743.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3202743/SRX3202743.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。