Job ID = 2010171 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 17,445,822 reads read : 17,445,822 reads written : 17,445,822 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:12 17445822 reads; of these: 17445822 (100.00%) were unpaired; of these: 517873 (2.97%) aligned 0 times 14686984 (84.19%) aligned exactly 1 time 2240965 (12.85%) aligned >1 times 97.03% overall alignment rate Time searching: 00:03:12 Overall time: 00:03:12 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 7526621 / 16927949 = 0.4446 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 21:49:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2894840/SRX2894840.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2894840/SRX2894840.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2894840/SRX2894840.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2894840/SRX2894840.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:49:13: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:49:13: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:49:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2894840/SRX2894840.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2894840/SRX2894840.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2894840/SRX2894840.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2894840/SRX2894840.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:49:13: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:49:13: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:49:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2894840/SRX2894840.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2894840/SRX2894840.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2894840/SRX2894840.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2894840/SRX2894840.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:49:14: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:49:14: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:49:21: 1000000 INFO @ Fri, 05 Jul 2019 21:49:22: 1000000 INFO @ Fri, 05 Jul 2019 21:49:24: 1000000 INFO @ Fri, 05 Jul 2019 21:49:28: 2000000 INFO @ Fri, 05 Jul 2019 21:49:30: 2000000 INFO @ Fri, 05 Jul 2019 21:49:34: 2000000 INFO @ Fri, 05 Jul 2019 21:49:35: 3000000 INFO @ Fri, 05 Jul 2019 21:49:38: 3000000 INFO @ Fri, 05 Jul 2019 21:49:42: 4000000 INFO @ Fri, 05 Jul 2019 21:49:43: 3000000 INFO @ Fri, 05 Jul 2019 21:49:47: 4000000 INFO @ Fri, 05 Jul 2019 21:49:49: 5000000 INFO @ Fri, 05 Jul 2019 21:49:53: 4000000 INFO @ Fri, 05 Jul 2019 21:49:56: 5000000 INFO @ Fri, 05 Jul 2019 21:49:56: 6000000 INFO @ Fri, 05 Jul 2019 21:50:02: 5000000 INFO @ Fri, 05 Jul 2019 21:50:03: 7000000 INFO @ Fri, 05 Jul 2019 21:50:04: 6000000 INFO @ Fri, 05 Jul 2019 21:50:11: 8000000 INFO @ Fri, 05 Jul 2019 21:50:12: 6000000 INFO @ Fri, 05 Jul 2019 21:50:13: 7000000 INFO @ Fri, 05 Jul 2019 21:50:18: 9000000 INFO @ Fri, 05 Jul 2019 21:50:20: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:50:20: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:50:20: #1 total tags in treatment: 9401328 INFO @ Fri, 05 Jul 2019 21:50:20: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:50:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:50:21: #1 tags after filtering in treatment: 9401328 INFO @ Fri, 05 Jul 2019 21:50:21: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:50:21: #1 finished! INFO @ Fri, 05 Jul 2019 21:50:21: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:50:21: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:50:21: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:50:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:50:21: Process for pairing-model is terminated! INFO @ Fri, 05 Jul 2019 21:50:21: 8000000 cut: /home/okishinya/chipatlas/results/sacCer3/SRX2894840/SRX2894840.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2894840/SRX2894840.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2894840/SRX2894840.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2894840/SRX2894840.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:50:22: 7000000 INFO @ Fri, 05 Jul 2019 21:50:30: 9000000 INFO @ Fri, 05 Jul 2019 21:50:31: 8000000 INFO @ Fri, 05 Jul 2019 21:50:33: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:50:33: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:50:33: #1 total tags in treatment: 9401328 INFO @ Fri, 05 Jul 2019 21:50:33: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:50:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:50:34: #1 tags after filtering in treatment: 9401328 INFO @ Fri, 05 Jul 2019 21:50:34: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:50:34: #1 finished! INFO @ Fri, 05 Jul 2019 21:50:34: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:50:34: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:50:34: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:50:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:50:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2894840/SRX2894840.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2894840/SRX2894840.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2894840/SRX2894840.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2894840/SRX2894840.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:50:40: 9000000 INFO @ Fri, 05 Jul 2019 21:50:44: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:50:44: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:50:44: #1 total tags in treatment: 9401328 INFO @ Fri, 05 Jul 2019 21:50:44: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:50:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:50:44: #1 tags after filtering in treatment: 9401328 INFO @ Fri, 05 Jul 2019 21:50:44: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:50:44: #1 finished! INFO @ Fri, 05 Jul 2019 21:50:44: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:50:44: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:50:45: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:50:45: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:50:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2894840/SRX2894840.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2894840/SRX2894840.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2894840/SRX2894840.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2894840/SRX2894840.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。