Job ID = 7098378 SRX = SRX2797019 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 6848157 spots for SRR5527833/SRR5527833.sra Written 6848157 spots for SRR5527833/SRR5527833.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:58 6848157 reads; of these: 6848157 (100.00%) were unpaired; of these: 192961 (2.82%) aligned 0 times 5392027 (78.74%) aligned exactly 1 time 1263169 (18.45%) aligned >1 times 97.18% overall alignment rate Time searching: 00:00:58 Overall time: 00:00:58 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 3294506 / 6655196 = 0.4950 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 11:57:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2797019/SRX2797019.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2797019/SRX2797019.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2797019/SRX2797019.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2797019/SRX2797019.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 11:57:59: #1 read tag files... INFO @ Wed, 22 Jul 2020 11:57:59: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 11:58:04: 1000000 INFO @ Wed, 22 Jul 2020 11:58:10: 2000000 INFO @ Wed, 22 Jul 2020 11:58:15: 3000000 INFO @ Wed, 22 Jul 2020 11:58:18: #1 tag size is determined as 51 bps INFO @ Wed, 22 Jul 2020 11:58:18: #1 tag size = 51 INFO @ Wed, 22 Jul 2020 11:58:18: #1 total tags in treatment: 3360690 INFO @ Wed, 22 Jul 2020 11:58:18: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 11:58:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 11:58:18: #1 tags after filtering in treatment: 3360690 INFO @ Wed, 22 Jul 2020 11:58:18: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 11:58:18: #1 finished! INFO @ Wed, 22 Jul 2020 11:58:18: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 11:58:18: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 11:58:18: #2 number of paired peaks: 46 WARNING @ Wed, 22 Jul 2020 11:58:18: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 11:58:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2797019/SRX2797019.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797019/SRX2797019.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797019/SRX2797019.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797019/SRX2797019.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 11:58:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2797019/SRX2797019.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2797019/SRX2797019.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2797019/SRX2797019.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2797019/SRX2797019.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 11:58:29: #1 read tag files... INFO @ Wed, 22 Jul 2020 11:58:29: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 11:58:34: 1000000 INFO @ Wed, 22 Jul 2020 11:58:40: 2000000 INFO @ Wed, 22 Jul 2020 11:58:45: 3000000 INFO @ Wed, 22 Jul 2020 11:58:47: #1 tag size is determined as 51 bps INFO @ Wed, 22 Jul 2020 11:58:47: #1 tag size = 51 INFO @ Wed, 22 Jul 2020 11:58:47: #1 total tags in treatment: 3360690 INFO @ Wed, 22 Jul 2020 11:58:47: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 11:58:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 11:58:47: #1 tags after filtering in treatment: 3360690 INFO @ Wed, 22 Jul 2020 11:58:47: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 11:58:47: #1 finished! INFO @ Wed, 22 Jul 2020 11:58:47: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 11:58:47: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 11:58:47: #2 number of paired peaks: 46 WARNING @ Wed, 22 Jul 2020 11:58:47: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 11:58:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2797019/SRX2797019.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797019/SRX2797019.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797019/SRX2797019.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797019/SRX2797019.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 11:58:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2797019/SRX2797019.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2797019/SRX2797019.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2797019/SRX2797019.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2797019/SRX2797019.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 11:58:59: #1 read tag files... INFO @ Wed, 22 Jul 2020 11:58:59: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 11:59:03: 1000000 INFO @ Wed, 22 Jul 2020 11:59:08: 2000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 11:59:13: 3000000 INFO @ Wed, 22 Jul 2020 11:59:14: #1 tag size is determined as 51 bps INFO @ Wed, 22 Jul 2020 11:59:14: #1 tag size = 51 INFO @ Wed, 22 Jul 2020 11:59:14: #1 total tags in treatment: 3360690 INFO @ Wed, 22 Jul 2020 11:59:14: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 11:59:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 11:59:15: #1 tags after filtering in treatment: 3360690 INFO @ Wed, 22 Jul 2020 11:59:15: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 11:59:15: #1 finished! INFO @ Wed, 22 Jul 2020 11:59:15: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 11:59:15: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 11:59:15: #2 number of paired peaks: 46 WARNING @ Wed, 22 Jul 2020 11:59:15: Too few paired peaks (46) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 11:59:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2797019/SRX2797019.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797019/SRX2797019.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797019/SRX2797019.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797019/SRX2797019.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。