Job ID = 7097993 SRX = SRX2797005 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 7081980 spots for SRR5527819/SRR5527819.sra Written 7081980 spots for SRR5527819/SRR5527819.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:15 7081980 reads; of these: 7081980 (100.00%) were unpaired; of these: 65198 (0.92%) aligned 0 times 5926169 (83.68%) aligned exactly 1 time 1090613 (15.40%) aligned >1 times 99.08% overall alignment rate Time searching: 00:01:15 Overall time: 00:01:15 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 2605534 / 7016782 = 0.3713 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 11:50:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2797005/SRX2797005.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2797005/SRX2797005.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2797005/SRX2797005.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2797005/SRX2797005.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 11:50:08: #1 read tag files... INFO @ Wed, 22 Jul 2020 11:50:08: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 11:50:14: 1000000 INFO @ Wed, 22 Jul 2020 11:50:19: 2000000 INFO @ Wed, 22 Jul 2020 11:50:25: 3000000 INFO @ Wed, 22 Jul 2020 11:50:30: 4000000 INFO @ Wed, 22 Jul 2020 11:50:32: #1 tag size is determined as 51 bps INFO @ Wed, 22 Jul 2020 11:50:32: #1 tag size = 51 INFO @ Wed, 22 Jul 2020 11:50:32: #1 total tags in treatment: 4411248 INFO @ Wed, 22 Jul 2020 11:50:32: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 11:50:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 11:50:32: #1 tags after filtering in treatment: 4411248 INFO @ Wed, 22 Jul 2020 11:50:32: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 11:50:32: #1 finished! INFO @ Wed, 22 Jul 2020 11:50:32: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 11:50:32: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 11:50:33: #2 number of paired peaks: 34 WARNING @ Wed, 22 Jul 2020 11:50:33: Too few paired peaks (34) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 11:50:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2797005/SRX2797005.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797005/SRX2797005.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797005/SRX2797005.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797005/SRX2797005.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 11:50:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2797005/SRX2797005.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2797005/SRX2797005.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2797005/SRX2797005.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2797005/SRX2797005.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 11:50:38: #1 read tag files... INFO @ Wed, 22 Jul 2020 11:50:38: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 11:50:44: 1000000 INFO @ Wed, 22 Jul 2020 11:50:49: 2000000 INFO @ Wed, 22 Jul 2020 11:50:55: 3000000 INFO @ Wed, 22 Jul 2020 11:51:00: 4000000 INFO @ Wed, 22 Jul 2020 11:51:02: #1 tag size is determined as 51 bps INFO @ Wed, 22 Jul 2020 11:51:02: #1 tag size = 51 INFO @ Wed, 22 Jul 2020 11:51:02: #1 total tags in treatment: 4411248 INFO @ Wed, 22 Jul 2020 11:51:02: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 11:51:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 11:51:02: #1 tags after filtering in treatment: 4411248 INFO @ Wed, 22 Jul 2020 11:51:02: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 11:51:02: #1 finished! INFO @ Wed, 22 Jul 2020 11:51:02: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 11:51:02: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 11:51:02: #2 number of paired peaks: 34 WARNING @ Wed, 22 Jul 2020 11:51:02: Too few paired peaks (34) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 11:51:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2797005/SRX2797005.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797005/SRX2797005.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797005/SRX2797005.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797005/SRX2797005.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 11:51:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2797005/SRX2797005.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2797005/SRX2797005.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2797005/SRX2797005.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2797005/SRX2797005.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 11:51:08: #1 read tag files... INFO @ Wed, 22 Jul 2020 11:51:08: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 11:51:14: 1000000 INFO @ Wed, 22 Jul 2020 11:51:19: 2000000 INFO @ Wed, 22 Jul 2020 11:51:25: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 11:51:30: 4000000 INFO @ Wed, 22 Jul 2020 11:51:32: #1 tag size is determined as 51 bps INFO @ Wed, 22 Jul 2020 11:51:32: #1 tag size = 51 INFO @ Wed, 22 Jul 2020 11:51:32: #1 total tags in treatment: 4411248 INFO @ Wed, 22 Jul 2020 11:51:32: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 11:51:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 11:51:32: #1 tags after filtering in treatment: 4411248 INFO @ Wed, 22 Jul 2020 11:51:32: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 11:51:32: #1 finished! INFO @ Wed, 22 Jul 2020 11:51:32: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 11:51:32: #2 looking for paired plus/minus strand peaks... BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 11:51:33: #2 number of paired peaks: 34 WARNING @ Wed, 22 Jul 2020 11:51:33: Too few paired peaks (34) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 11:51:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2797005/SRX2797005.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797005/SRX2797005.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797005/SRX2797005.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797005/SRX2797005.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling