Job ID = 2010130 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 12,108,133 reads read : 12,108,133 reads written : 12,108,133 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:26 12108133 reads; of these: 12108133 (100.00%) were unpaired; of these: 5558340 (45.91%) aligned 0 times 5671032 (46.84%) aligned exactly 1 time 878761 (7.26%) aligned >1 times 54.09% overall alignment rate Time searching: 00:02:26 Overall time: 00:02:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1548998 / 6549793 = 0.2365 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 21:30:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2772205/SRX2772205.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2772205/SRX2772205.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2772205/SRX2772205.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2772205/SRX2772205.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:30:39: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:30:39: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:30:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2772205/SRX2772205.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2772205/SRX2772205.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2772205/SRX2772205.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2772205/SRX2772205.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:30:40: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:30:40: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:30:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2772205/SRX2772205.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2772205/SRX2772205.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2772205/SRX2772205.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2772205/SRX2772205.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:30:41: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:30:41: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:30:46: 1000000 INFO @ Fri, 05 Jul 2019 21:30:48: 1000000 INFO @ Fri, 05 Jul 2019 21:30:50: 1000000 INFO @ Fri, 05 Jul 2019 21:30:53: 2000000 INFO @ Fri, 05 Jul 2019 21:30:55: 2000000 INFO @ Fri, 05 Jul 2019 21:30:59: 2000000 INFO @ Fri, 05 Jul 2019 21:31:00: 3000000 INFO @ Fri, 05 Jul 2019 21:31:03: 3000000 INFO @ Fri, 05 Jul 2019 21:31:06: 4000000 INFO @ Fri, 05 Jul 2019 21:31:08: 3000000 INFO @ Fri, 05 Jul 2019 21:31:10: 4000000 INFO @ Fri, 05 Jul 2019 21:31:13: 5000000 INFO @ Fri, 05 Jul 2019 21:31:13: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 21:31:13: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 21:31:13: #1 total tags in treatment: 5000795 INFO @ Fri, 05 Jul 2019 21:31:13: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:31:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:31:13: #1 tags after filtering in treatment: 5000795 INFO @ Fri, 05 Jul 2019 21:31:13: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:31:13: #1 finished! INFO @ Fri, 05 Jul 2019 21:31:13: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:31:13: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:31:14: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:31:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:31:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2772205/SRX2772205.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2772205/SRX2772205.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2772205/SRX2772205.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2772205/SRX2772205.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:31:16: 4000000 INFO @ Fri, 05 Jul 2019 21:31:17: 5000000 INFO @ Fri, 05 Jul 2019 21:31:17: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 21:31:17: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 21:31:17: #1 total tags in treatment: 5000795 INFO @ Fri, 05 Jul 2019 21:31:17: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:31:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:31:17: #1 tags after filtering in treatment: 5000795 INFO @ Fri, 05 Jul 2019 21:31:17: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:31:17: #1 finished! INFO @ Fri, 05 Jul 2019 21:31:17: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:31:17: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:31:18: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:31:18: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:31:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2772205/SRX2772205.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2772205/SRX2772205.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2772205/SRX2772205.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2772205/SRX2772205.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:31:24: 5000000 INFO @ Fri, 05 Jul 2019 21:31:24: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 21:31:24: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 21:31:24: #1 total tags in treatment: 5000795 INFO @ Fri, 05 Jul 2019 21:31:24: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:31:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:31:24: #1 tags after filtering in treatment: 5000795 INFO @ Fri, 05 Jul 2019 21:31:24: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:31:24: #1 finished! INFO @ Fri, 05 Jul 2019 21:31:24: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:31:24: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:31:25: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:31:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:31:25: Process for pairing-model is terminated! BedGraph に変換しました。 cut: /home/okishinya/chipatlas/results/sacCer3/SRX2772205/SRX2772205.20_peaks.narrowPeak: No such file or directory BigWig に変換中... pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2772205/SRX2772205.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2772205/SRX2772205.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2772205/SRX2772205.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。