Job ID = 9160147 sra ファイルのダウンロード中... Completed: 480571K bytes transferred in 21 seconds (186395K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 16003657 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2737261/SRR5448394.sra Written 16003657 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:14 16003657 reads; of these: 16003657 (100.00%) were unpaired; of these: 633198 (3.96%) aligned 0 times 11953053 (74.69%) aligned exactly 1 time 3417406 (21.35%) aligned >1 times 96.04% overall alignment rate Time searching: 00:03:14 Overall time: 00:03:14 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5631880 / 15370459 = 0.3664 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 02:04:14: # Command line: callpeak -t SRX2737261.bam -f BAM -g 12100000 -n SRX2737261.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2737261.10 # format = BAM # ChIP-seq file = ['SRX2737261.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 02:04:14: #1 read tag files... INFO @ Wed, 28 Jun 2017 02:04:14: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 02:04:14: # Command line: callpeak -t SRX2737261.bam -f BAM -g 12100000 -n SRX2737261.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2737261.20 # format = BAM # ChIP-seq file = ['SRX2737261.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 02:04:14: #1 read tag files... INFO @ Wed, 28 Jun 2017 02:04:14: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 02:04:14: # Command line: callpeak -t SRX2737261.bam -f BAM -g 12100000 -n SRX2737261.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2737261.05 # format = BAM # ChIP-seq file = ['SRX2737261.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 02:04:14: #1 read tag files... INFO @ Wed, 28 Jun 2017 02:04:14: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 02:04:21: 1000000 INFO @ Wed, 28 Jun 2017 02:04:21: 1000000 INFO @ Wed, 28 Jun 2017 02:04:22: 1000000 INFO @ Wed, 28 Jun 2017 02:04:28: 2000000 INFO @ Wed, 28 Jun 2017 02:04:28: 2000000 INFO @ Wed, 28 Jun 2017 02:04:30: 2000000 INFO @ Wed, 28 Jun 2017 02:04:34: 3000000 INFO @ Wed, 28 Jun 2017 02:04:35: 3000000 INFO @ Wed, 28 Jun 2017 02:04:36: 3000000 INFO @ Wed, 28 Jun 2017 02:04:41: 4000000 INFO @ Wed, 28 Jun 2017 02:04:42: 4000000 INFO @ Wed, 28 Jun 2017 02:04:43: 4000000 INFO @ Wed, 28 Jun 2017 02:04:48: 5000000 INFO @ Wed, 28 Jun 2017 02:04:48: 5000000 INFO @ Wed, 28 Jun 2017 02:04:50: 5000000 INFO @ Wed, 28 Jun 2017 02:04:54: 6000000 INFO @ Wed, 28 Jun 2017 02:04:55: 6000000 INFO @ Wed, 28 Jun 2017 02:04:57: 6000000 INFO @ Wed, 28 Jun 2017 02:05:01: 7000000 INFO @ Wed, 28 Jun 2017 02:05:02: 7000000 INFO @ Wed, 28 Jun 2017 02:05:04: 7000000 INFO @ Wed, 28 Jun 2017 02:05:07: 8000000 INFO @ Wed, 28 Jun 2017 02:05:09: 8000000 INFO @ Wed, 28 Jun 2017 02:05:11: 8000000 INFO @ Wed, 28 Jun 2017 02:05:14: 9000000 INFO @ Wed, 28 Jun 2017 02:05:16: 9000000 INFO @ Wed, 28 Jun 2017 02:05:18: 9000000 INFO @ Wed, 28 Jun 2017 02:05:19: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 02:05:19: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 02:05:19: #1 total tags in treatment: 9738579 INFO @ Wed, 28 Jun 2017 02:05:19: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 02:05:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 02:05:19: #1 tags after filtering in treatment: 9738579 INFO @ Wed, 28 Jun 2017 02:05:19: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 02:05:19: #1 finished! INFO @ Wed, 28 Jun 2017 02:05:19: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 02:05:19: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 02:05:20: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 02:05:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 02:05:20: Process for pairing-model is terminated! cat: SRX2737261.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2737261.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2737261.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2737261.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 02:05:21: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 02:05:21: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 02:05:21: #1 total tags in treatment: 9738579 INFO @ Wed, 28 Jun 2017 02:05:21: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 02:05:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 02:05:21: #1 tags after filtering in treatment: 9738579 INFO @ Wed, 28 Jun 2017 02:05:21: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 02:05:21: #1 finished! INFO @ Wed, 28 Jun 2017 02:05:21: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 02:05:21: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 02:05:22: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 02:05:22: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 02:05:22: Process for pairing-model is terminated! cat: SRX2737261.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2737261.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2737261.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2737261.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 02:05:23: #1 tag size is determined as 50 bps INFO @ Wed, 28 Jun 2017 02:05:23: #1 tag size = 50 INFO @ Wed, 28 Jun 2017 02:05:23: #1 total tags in treatment: 9738579 INFO @ Wed, 28 Jun 2017 02:05:23: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 02:05:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 02:05:23: #1 tags after filtering in treatment: 9738579 INFO @ Wed, 28 Jun 2017 02:05:23: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 02:05:23: #1 finished! INFO @ Wed, 28 Jun 2017 02:05:23: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 02:05:23: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 02:05:23: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 02:05:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 02:05:23: Process for pairing-model is terminated! cat: SRX2737261.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2737261.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2737261.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2737261.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。