Job ID = 9162223 sra ファイルのダウンロード中... Completed: 799293K bytes transferred in 9 seconds (697200K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 28713570 spots for /home/okishinya/chipatlas/results/sacCer3/SRX1738695/SRR3471226.sra Written 28713570 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:17 28713570 reads; of these: 28713570 (100.00%) were unpaired; of these: 1525107 (5.31%) aligned 0 times 23221150 (80.87%) aligned exactly 1 time 3967313 (13.82%) aligned >1 times 94.69% overall alignment rate Time searching: 00:06:17 Overall time: 00:06:17 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 13269684 / 27188463 = 0.4881 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 07:05:23: # Command line: callpeak -t SRX1738695.bam -f BAM -g 12100000 -n SRX1738695.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1738695.20 # format = BAM # ChIP-seq file = ['SRX1738695.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 07:05:23: #1 read tag files... INFO @ Wed, 28 Jun 2017 07:05:23: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 07:05:23: # Command line: callpeak -t SRX1738695.bam -f BAM -g 12100000 -n SRX1738695.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1738695.05 # format = BAM # ChIP-seq file = ['SRX1738695.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 07:05:23: #1 read tag files... INFO @ Wed, 28 Jun 2017 07:05:23: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 07:05:23: # Command line: callpeak -t SRX1738695.bam -f BAM -g 12100000 -n SRX1738695.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1738695.10 # format = BAM # ChIP-seq file = ['SRX1738695.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 07:05:23: #1 read tag files... INFO @ Wed, 28 Jun 2017 07:05:23: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 07:05:30: 1000000 INFO @ Wed, 28 Jun 2017 07:05:30: 1000000 INFO @ Wed, 28 Jun 2017 07:05:30: 1000000 INFO @ Wed, 28 Jun 2017 07:05:36: 2000000 INFO @ Wed, 28 Jun 2017 07:05:36: 2000000 INFO @ Wed, 28 Jun 2017 07:05:36: 2000000 INFO @ Wed, 28 Jun 2017 07:05:42: 3000000 INFO @ Wed, 28 Jun 2017 07:05:42: 3000000 INFO @ Wed, 28 Jun 2017 07:05:43: 3000000 INFO @ Wed, 28 Jun 2017 07:05:49: 4000000 INFO @ Wed, 28 Jun 2017 07:05:49: 4000000 INFO @ Wed, 28 Jun 2017 07:05:50: 4000000 INFO @ Wed, 28 Jun 2017 07:05:55: 5000000 INFO @ Wed, 28 Jun 2017 07:05:55: 5000000 INFO @ Wed, 28 Jun 2017 07:05:56: 5000000 INFO @ Wed, 28 Jun 2017 07:06:02: 6000000 INFO @ Wed, 28 Jun 2017 07:06:02: 6000000 INFO @ Wed, 28 Jun 2017 07:06:04: 6000000 INFO @ Wed, 28 Jun 2017 07:06:09: 7000000 INFO @ Wed, 28 Jun 2017 07:06:09: 7000000 INFO @ Wed, 28 Jun 2017 07:06:11: 7000000 INFO @ Wed, 28 Jun 2017 07:06:15: 8000000 INFO @ Wed, 28 Jun 2017 07:06:16: 8000000 INFO @ Wed, 28 Jun 2017 07:06:18: 8000000 INFO @ Wed, 28 Jun 2017 07:06:22: 9000000 INFO @ Wed, 28 Jun 2017 07:06:23: 9000000 INFO @ Wed, 28 Jun 2017 07:06:25: 9000000 INFO @ Wed, 28 Jun 2017 07:06:29: 10000000 INFO @ Wed, 28 Jun 2017 07:06:30: 10000000 INFO @ Wed, 28 Jun 2017 07:06:33: 10000000 INFO @ Wed, 28 Jun 2017 07:06:36: 11000000 INFO @ Wed, 28 Jun 2017 07:06:37: 11000000 INFO @ Wed, 28 Jun 2017 07:06:40: 11000000 INFO @ Wed, 28 Jun 2017 07:06:43: 12000000 INFO @ Wed, 28 Jun 2017 07:06:44: 12000000 INFO @ Wed, 28 Jun 2017 07:06:47: 12000000 INFO @ Wed, 28 Jun 2017 07:06:50: 13000000 INFO @ Wed, 28 Jun 2017 07:06:52: 13000000 INFO @ Wed, 28 Jun 2017 07:06:55: 13000000 INFO @ Wed, 28 Jun 2017 07:06:57: #1 tag size is determined as 49 bps INFO @ Wed, 28 Jun 2017 07:06:57: #1 tag size = 49 INFO @ Wed, 28 Jun 2017 07:06:57: #1 total tags in treatment: 13918779 INFO @ Wed, 28 Jun 2017 07:06:57: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 07:06:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 07:06:57: #1 tags after filtering in treatment: 13918779 INFO @ Wed, 28 Jun 2017 07:06:57: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 07:06:57: #1 finished! INFO @ Wed, 28 Jun 2017 07:06:57: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 07:06:57: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 07:06:58: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 07:06:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 07:06:58: Process for pairing-model is terminated! cat: SRX1738695.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1738695.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1738695.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1738695.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 07:06:58: #1 tag size is determined as 49 bps INFO @ Wed, 28 Jun 2017 07:06:58: #1 tag size = 49 INFO @ Wed, 28 Jun 2017 07:06:58: #1 total tags in treatment: 13918779 INFO @ Wed, 28 Jun 2017 07:06:58: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 07:06:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 07:06:58: #1 tags after filtering in treatment: 13918779 INFO @ Wed, 28 Jun 2017 07:06:58: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 07:06:58: #1 finished! INFO @ Wed, 28 Jun 2017 07:06:58: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 07:06:58: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 07:06:59: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 07:06:59: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 07:06:59: Process for pairing-model is terminated! cat: SRX1738695.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1738695.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1738695.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1738695.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 07:07:01: #1 tag size is determined as 49 bps INFO @ Wed, 28 Jun 2017 07:07:01: #1 tag size = 49 INFO @ Wed, 28 Jun 2017 07:07:01: #1 total tags in treatment: 13918779 INFO @ Wed, 28 Jun 2017 07:07:01: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 07:07:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 07:07:01: #1 tags after filtering in treatment: 13918779 INFO @ Wed, 28 Jun 2017 07:07:01: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 07:07:01: #1 finished! INFO @ Wed, 28 Jun 2017 07:07:01: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 07:07:01: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 07:07:02: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 07:07:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 07:07:02: Process for pairing-model is terminated! cat: SRX1738695.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1738695.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1738695.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1738695.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。