Job ID = 9162214 sra ファイルのダウンロード中... Completed: 880688K bytes transferred in 10 seconds (714473K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 30359839 spots for /home/okishinya/chipatlas/results/sacCer3/SRX1738686/SRR3471217.sra Written 30359839 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:23 30359839 reads; of these: 30359839 (100.00%) were unpaired; of these: 1072498 (3.53%) aligned 0 times 24998265 (82.34%) aligned exactly 1 time 4289076 (14.13%) aligned >1 times 96.47% overall alignment rate Time searching: 00:05:23 Overall time: 00:05:23 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 15450740 / 29287341 = 0.5276 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Wed, 28 Jun 2017 07:03:59: # Command line: callpeak -t SRX1738686.bam -f BAM -g 12100000 -n SRX1738686.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1738686.05 # format = BAM # ChIP-seq file = ['SRX1738686.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 07:03:59: #1 read tag files... INFO @ Wed, 28 Jun 2017 07:03:59: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 07:03:59: # Command line: callpeak -t SRX1738686.bam -f BAM -g 12100000 -n SRX1738686.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1738686.10 # format = BAM # ChIP-seq file = ['SRX1738686.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 07:03:59: #1 read tag files... INFO @ Wed, 28 Jun 2017 07:03:59: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 07:03:59: # Command line: callpeak -t SRX1738686.bam -f BAM -g 12100000 -n SRX1738686.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1738686.20 # format = BAM # ChIP-seq file = ['SRX1738686.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 28 Jun 2017 07:03:59: #1 read tag files... INFO @ Wed, 28 Jun 2017 07:03:59: #1 read treatment tags... INFO @ Wed, 28 Jun 2017 07:04:05: 1000000 INFO @ Wed, 28 Jun 2017 07:04:06: 1000000 INFO @ Wed, 28 Jun 2017 07:04:06: 1000000 INFO @ Wed, 28 Jun 2017 07:04:12: 2000000 INFO @ Wed, 28 Jun 2017 07:04:13: 2000000 INFO @ Wed, 28 Jun 2017 07:04:13: 2000000 INFO @ Wed, 28 Jun 2017 07:04:19: 3000000 INFO @ Wed, 28 Jun 2017 07:04:20: 3000000 INFO @ Wed, 28 Jun 2017 07:04:20: 3000000 INFO @ Wed, 28 Jun 2017 07:04:25: 4000000 INFO @ Wed, 28 Jun 2017 07:04:28: 4000000 INFO @ Wed, 28 Jun 2017 07:04:28: 4000000 INFO @ Wed, 28 Jun 2017 07:04:32: 5000000 INFO @ Wed, 28 Jun 2017 07:04:35: 5000000 INFO @ Wed, 28 Jun 2017 07:04:35: 5000000 INFO @ Wed, 28 Jun 2017 07:04:39: 6000000 INFO @ Wed, 28 Jun 2017 07:04:42: 6000000 INFO @ Wed, 28 Jun 2017 07:04:42: 6000000 INFO @ Wed, 28 Jun 2017 07:04:45: 7000000 INFO @ Wed, 28 Jun 2017 07:04:50: 7000000 INFO @ Wed, 28 Jun 2017 07:04:50: 7000000 INFO @ Wed, 28 Jun 2017 07:04:52: 8000000 INFO @ Wed, 28 Jun 2017 07:04:57: 8000000 INFO @ Wed, 28 Jun 2017 07:04:57: 8000000 INFO @ Wed, 28 Jun 2017 07:04:59: 9000000 INFO @ Wed, 28 Jun 2017 07:05:04: 9000000 INFO @ Wed, 28 Jun 2017 07:05:04: 9000000 INFO @ Wed, 28 Jun 2017 07:05:05: 10000000 INFO @ Wed, 28 Jun 2017 07:05:11: 10000000 INFO @ Wed, 28 Jun 2017 07:05:11: 10000000 INFO @ Wed, 28 Jun 2017 07:05:12: 11000000 INFO @ Wed, 28 Jun 2017 07:05:19: 11000000 INFO @ Wed, 28 Jun 2017 07:05:19: 11000000 INFO @ Wed, 28 Jun 2017 07:05:19: 12000000 INFO @ Wed, 28 Jun 2017 07:05:25: 13000000 INFO @ Wed, 28 Jun 2017 07:05:26: 12000000 INFO @ Wed, 28 Jun 2017 07:05:26: 12000000 INFO @ Wed, 28 Jun 2017 07:05:31: #1 tag size is determined as 49 bps INFO @ Wed, 28 Jun 2017 07:05:31: #1 tag size = 49 INFO @ Wed, 28 Jun 2017 07:05:31: #1 total tags in treatment: 13836601 INFO @ Wed, 28 Jun 2017 07:05:31: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 07:05:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 07:05:31: #1 tags after filtering in treatment: 13836601 INFO @ Wed, 28 Jun 2017 07:05:31: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 07:05:31: #1 finished! INFO @ Wed, 28 Jun 2017 07:05:31: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 07:05:31: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 07:05:32: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 07:05:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 07:05:32: Process for pairing-model is terminated! cat: SRX1738686.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1738686.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1738686.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1738686.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Wed, 28 Jun 2017 07:05:33: 13000000 INFO @ Wed, 28 Jun 2017 07:05:33: 13000000 INFO @ Wed, 28 Jun 2017 07:05:39: #1 tag size is determined as 49 bps INFO @ Wed, 28 Jun 2017 07:05:39: #1 tag size is determined as 49 bps INFO @ Wed, 28 Jun 2017 07:05:39: #1 tag size = 49 INFO @ Wed, 28 Jun 2017 07:05:39: #1 tag size = 49 INFO @ Wed, 28 Jun 2017 07:05:39: #1 total tags in treatment: 13836601 INFO @ Wed, 28 Jun 2017 07:05:39: #1 total tags in treatment: 13836601 INFO @ Wed, 28 Jun 2017 07:05:39: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 07:05:39: #1 user defined the maximum tags... INFO @ Wed, 28 Jun 2017 07:05:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 07:05:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 28 Jun 2017 07:05:39: #1 tags after filtering in treatment: 13836601 INFO @ Wed, 28 Jun 2017 07:05:39: #1 tags after filtering in treatment: 13836601 INFO @ Wed, 28 Jun 2017 07:05:39: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 07:05:39: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 28 Jun 2017 07:05:39: #1 finished! INFO @ Wed, 28 Jun 2017 07:05:39: #1 finished! INFO @ Wed, 28 Jun 2017 07:05:39: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 07:05:39: #2 Build Peak Model... INFO @ Wed, 28 Jun 2017 07:05:39: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 07:05:39: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 28 Jun 2017 07:05:40: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 07:05:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 07:05:40: Process for pairing-model is terminated! INFO @ Wed, 28 Jun 2017 07:05:40: #2 number of paired peaks: 0 WARNING @ Wed, 28 Jun 2017 07:05:40: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 28 Jun 2017 07:05:40: Process for pairing-model is terminated! cat: SRX1738686.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません cat: SRX1738686.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1738686.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1738686.20_*.xls': そのようなファイルやディレクトリはありませんrm: cannot remove `SRX1738686.10_model.r': そのようなファイルやディレクトリはありませんrm: cannot remove `SRX1738686.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1738686.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1738686.10_peaks.narrowPeak': そのようなファイルやディレクトリはありませんCompletedMACS2peakCalling CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。