Job ID = 2009695 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 22,857,086 reads read : 22,857,086 reads written : 22,857,086 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR364348.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:49 22857086 reads; of these: 22857086 (100.00%) were unpaired; of these: 10762438 (47.09%) aligned 0 times 10045709 (43.95%) aligned exactly 1 time 2048939 (8.96%) aligned >1 times 52.91% overall alignment rate Time searching: 00:01:49 Overall time: 00:01:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 5385294 / 12094648 = 0.4453 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Fri, 05 Jul 2019 19:37:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX105562/SRX105562.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX105562/SRX105562.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX105562/SRX105562.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX105562/SRX105562.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:37:15: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:37:15: #1 read treatment tags... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 19:37:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX105562/SRX105562.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX105562/SRX105562.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX105562/SRX105562.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX105562/SRX105562.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:37:16: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:37:16: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:37:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX105562/SRX105562.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX105562/SRX105562.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX105562/SRX105562.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX105562/SRX105562.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:37:17: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:37:17: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:37:20: 1000000 INFO @ Fri, 05 Jul 2019 19:37:22: 1000000 INFO @ Fri, 05 Jul 2019 19:37:23: 1000000 INFO @ Fri, 05 Jul 2019 19:37:26: 2000000 INFO @ Fri, 05 Jul 2019 19:37:28: 2000000 INFO @ Fri, 05 Jul 2019 19:37:29: 2000000 INFO @ Fri, 05 Jul 2019 19:37:31: 3000000 INFO @ Fri, 05 Jul 2019 19:37:34: 3000000 INFO @ Fri, 05 Jul 2019 19:37:35: 3000000 INFO @ Fri, 05 Jul 2019 19:37:36: 4000000 INFO @ Fri, 05 Jul 2019 19:37:40: 4000000 INFO @ Fri, 05 Jul 2019 19:37:41: 4000000 INFO @ Fri, 05 Jul 2019 19:37:41: 5000000 INFO @ Fri, 05 Jul 2019 19:37:46: 5000000 INFO @ Fri, 05 Jul 2019 19:37:46: 6000000 INFO @ Fri, 05 Jul 2019 19:37:47: 5000000 INFO @ Fri, 05 Jul 2019 19:37:50: #1 tag size is determined as 40 bps INFO @ Fri, 05 Jul 2019 19:37:50: #1 tag size = 40 INFO @ Fri, 05 Jul 2019 19:37:50: #1 total tags in treatment: 6709354 INFO @ Fri, 05 Jul 2019 19:37:50: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:37:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:37:50: #1 tags after filtering in treatment: 6709354 INFO @ Fri, 05 Jul 2019 19:37:50: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:37:50: #1 finished! INFO @ Fri, 05 Jul 2019 19:37:50: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:37:50: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:37:50: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:37:50: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:37:50: Process for pairing-model is terminated! INFO @ Fri, 05 Jul 2019 19:37:52: 6000000 INFO @ Fri, 05 Jul 2019 19:37:52: 6000000 cut: /home/okishinya/chipatlas/results/sacCer3/SRX105562/SRX105562.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX105562/SRX105562.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX105562/SRX105562.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX105562/SRX105562.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 19:37:55: #1 tag size is determined as 40 bps INFO @ Fri, 05 Jul 2019 19:37:55: #1 tag size = 40 INFO @ Fri, 05 Jul 2019 19:37:55: #1 total tags in treatment: 6709354 INFO @ Fri, 05 Jul 2019 19:37:55: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:37:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:37:56: #1 tags after filtering in treatment: 6709354 INFO @ Fri, 05 Jul 2019 19:37:56: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:37:56: #1 finished! INFO @ Fri, 05 Jul 2019 19:37:56: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:37:56: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:37:56: #1 tag size is determined as 40 bps INFO @ Fri, 05 Jul 2019 19:37:56: #1 tag size = 40 INFO @ Fri, 05 Jul 2019 19:37:56: #1 total tags in treatment: 6709354 INFO @ Fri, 05 Jul 2019 19:37:56: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:37:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:37:56: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:37:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:37:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX105562/SRX105562.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX105562/SRX105562.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX105562/SRX105562.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX105562/SRX105562.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 19:37:56: #1 tags after filtering in treatment: 6709354 INFO @ Fri, 05 Jul 2019 19:37:56: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:37:56: #1 finished! INFO @ Fri, 05 Jul 2019 19:37:56: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:37:56: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:37:56: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:37:56: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:37:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX105562/SRX105562.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX105562/SRX105562.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX105562/SRX105562.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX105562/SRX105562.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。