Job ID = 2009689 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 16,021,536 reads read : 16,021,536 reads written : 16,021,536 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR364345.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:49 16021536 reads; of these: 16021536 (100.00%) were unpaired; of these: 1302882 (8.13%) aligned 0 times 12380166 (77.27%) aligned exactly 1 time 2338488 (14.60%) aligned >1 times 91.87% overall alignment rate Time searching: 00:07:49 Overall time: 00:07:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 5339225 / 14718654 = 0.3628 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 19:40:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX105559/SRX105559.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX105559/SRX105559.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX105559/SRX105559.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX105559/SRX105559.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:40:51: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:40:51: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:40:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX105559/SRX105559.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX105559/SRX105559.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX105559/SRX105559.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX105559/SRX105559.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:40:52: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:40:52: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:40:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX105559/SRX105559.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX105559/SRX105559.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX105559/SRX105559.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX105559/SRX105559.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:40:53: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:40:53: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:40:58: 1000000 INFO @ Fri, 05 Jul 2019 19:41:01: 1000000 INFO @ Fri, 05 Jul 2019 19:41:02: 1000000 INFO @ Fri, 05 Jul 2019 19:41:05: 2000000 INFO @ Fri, 05 Jul 2019 19:41:09: 2000000 INFO @ Fri, 05 Jul 2019 19:41:10: 2000000 INFO @ Fri, 05 Jul 2019 19:41:13: 3000000 INFO @ Fri, 05 Jul 2019 19:41:17: 3000000 INFO @ Fri, 05 Jul 2019 19:41:18: 3000000 INFO @ Fri, 05 Jul 2019 19:41:20: 4000000 INFO @ Fri, 05 Jul 2019 19:41:25: 4000000 INFO @ Fri, 05 Jul 2019 19:41:26: 4000000 INFO @ Fri, 05 Jul 2019 19:41:27: 5000000 INFO @ Fri, 05 Jul 2019 19:41:33: 5000000 INFO @ Fri, 05 Jul 2019 19:41:34: 6000000 INFO @ Fri, 05 Jul 2019 19:41:34: 5000000 INFO @ Fri, 05 Jul 2019 19:41:41: 6000000 INFO @ Fri, 05 Jul 2019 19:41:41: 7000000 INFO @ Fri, 05 Jul 2019 19:41:43: 6000000 INFO @ Fri, 05 Jul 2019 19:41:48: 8000000 INFO @ Fri, 05 Jul 2019 19:41:49: 7000000 INFO @ Fri, 05 Jul 2019 19:41:51: 7000000 INFO @ Fri, 05 Jul 2019 19:41:55: 9000000 INFO @ Fri, 05 Jul 2019 19:41:56: 8000000 INFO @ Fri, 05 Jul 2019 19:41:57: #1 tag size is determined as 40 bps INFO @ Fri, 05 Jul 2019 19:41:57: #1 tag size = 40 INFO @ Fri, 05 Jul 2019 19:41:57: #1 total tags in treatment: 9379429 INFO @ Fri, 05 Jul 2019 19:41:57: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:41:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:41:58: #1 tags after filtering in treatment: 9379429 INFO @ Fri, 05 Jul 2019 19:41:58: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:41:58: #1 finished! INFO @ Fri, 05 Jul 2019 19:41:58: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:41:58: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:41:58: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:41:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:41:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX105559/SRX105559.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX105559/SRX105559.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX105559/SRX105559.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX105559/SRX105559.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 19:41:59: 8000000 INFO @ Fri, 05 Jul 2019 19:42:04: 9000000 INFO @ Fri, 05 Jul 2019 19:42:07: 9000000 INFO @ Fri, 05 Jul 2019 19:42:07: #1 tag size is determined as 40 bps INFO @ Fri, 05 Jul 2019 19:42:07: #1 tag size = 40 INFO @ Fri, 05 Jul 2019 19:42:07: #1 total tags in treatment: 9379429 INFO @ Fri, 05 Jul 2019 19:42:07: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:42:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:42:07: #1 tags after filtering in treatment: 9379429 INFO @ Fri, 05 Jul 2019 19:42:07: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:42:07: #1 finished! INFO @ Fri, 05 Jul 2019 19:42:07: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:42:07: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:42:08: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:42:08: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:42:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX105559/SRX105559.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX105559/SRX105559.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX105559/SRX105559.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX105559/SRX105559.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 19:42:10: #1 tag size is determined as 40 bps INFO @ Fri, 05 Jul 2019 19:42:10: #1 tag size = 40 INFO @ Fri, 05 Jul 2019 19:42:10: #1 total tags in treatment: 9379429 INFO @ Fri, 05 Jul 2019 19:42:10: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:42:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:42:10: #1 tags after filtering in treatment: 9379429 INFO @ Fri, 05 Jul 2019 19:42:10: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:42:10: #1 finished! INFO @ Fri, 05 Jul 2019 19:42:10: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:42:10: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:42:11: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 19:42:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:42:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX105559/SRX105559.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX105559/SRX105559.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX105559/SRX105559.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX105559/SRX105559.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。