Job ID = 2009000 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 5,067,211 reads read : 5,067,211 reads written : 5,067,211 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/ERR637591.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:46 5067211 reads; of these: 5067211 (100.00%) were unpaired; of these: 123688 (2.44%) aligned 0 times 4192247 (82.73%) aligned exactly 1 time 751276 (14.83%) aligned >1 times 97.56% overall alignment rate Time searching: 00:00:46 Overall time: 00:00:46 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 891304 / 4943523 = 0.1803 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 19:06:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX594019/ERX594019.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX594019/ERX594019.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX594019/ERX594019.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX594019/ERX594019.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:06:27: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:06:27: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:06:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX594019/ERX594019.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX594019/ERX594019.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX594019/ERX594019.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX594019/ERX594019.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:06:28: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:06:28: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:06:34: 1000000 INFO @ Fri, 05 Jul 2019 19:06:34: 1000000 INFO @ Fri, 05 Jul 2019 19:06:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX594019/ERX594019.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX594019/ERX594019.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX594019/ERX594019.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX594019/ERX594019.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 19:06:37: #1 read tag files... INFO @ Fri, 05 Jul 2019 19:06:37: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 19:06:41: 2000000 INFO @ Fri, 05 Jul 2019 19:06:41: 2000000 INFO @ Fri, 05 Jul 2019 19:06:43: 1000000 INFO @ Fri, 05 Jul 2019 19:06:47: 3000000 INFO @ Fri, 05 Jul 2019 19:06:49: 3000000 INFO @ Fri, 05 Jul 2019 19:06:50: 2000000 INFO @ Fri, 05 Jul 2019 19:06:53: 4000000 INFO @ Fri, 05 Jul 2019 19:06:53: #1 tag size is determined as 40 bps INFO @ Fri, 05 Jul 2019 19:06:53: #1 tag size = 40 INFO @ Fri, 05 Jul 2019 19:06:53: #1 total tags in treatment: 4052219 INFO @ Fri, 05 Jul 2019 19:06:53: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:06:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:06:53: #1 tags after filtering in treatment: 4052219 INFO @ Fri, 05 Jul 2019 19:06:53: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:06:53: #1 finished! INFO @ Fri, 05 Jul 2019 19:06:53: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:06:53: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:06:54: #2 number of paired peaks: 28 WARNING @ Fri, 05 Jul 2019 19:06:54: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:06:54: Process for pairing-model is terminated! INFO @ Fri, 05 Jul 2019 19:06:56: 3000000 INFO @ Fri, 05 Jul 2019 19:06:57: 4000000 INFO @ Fri, 05 Jul 2019 19:06:58: #1 tag size is determined as 40 bps INFO @ Fri, 05 Jul 2019 19:06:58: #1 tag size = 40 INFO @ Fri, 05 Jul 2019 19:06:58: #1 total tags in treatment: 4052219 INFO @ Fri, 05 Jul 2019 19:06:58: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:06:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:06:58: #1 tags after filtering in treatment: 4052219 INFO @ Fri, 05 Jul 2019 19:06:58: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:06:58: #1 finished! INFO @ Fri, 05 Jul 2019 19:06:58: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:06:58: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:06:58: #2 number of paired peaks: 28 WARNING @ Fri, 05 Jul 2019 19:06:58: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:06:58: Process for pairing-model is terminated! INFO @ Fri, 05 Jul 2019 19:07:02: 4000000 INFO @ Fri, 05 Jul 2019 19:07:03: #1 tag size is determined as 40 bps INFO @ Fri, 05 Jul 2019 19:07:03: #1 tag size = 40 INFO @ Fri, 05 Jul 2019 19:07:03: #1 total tags in treatment: 4052219 INFO @ Fri, 05 Jul 2019 19:07:03: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 19:07:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 19:07:03: #1 tags after filtering in treatment: 4052219 INFO @ Fri, 05 Jul 2019 19:07:03: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 19:07:03: #1 finished! INFO @ Fri, 05 Jul 2019 19:07:03: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 19:07:03: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 19:07:03: #2 number of paired peaks: 28 WARNING @ Fri, 05 Jul 2019 19:07:03: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 19:07:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/ERX594019/ERX594019.10_peaks.narrowPeak: No such file or directory cut: /home/okishinya/chipatlas/results/sacCer3/ERX594019/ERX594019.20_peaks.narrowPeakcut: : No such file or directory/home/okishinya/chipatlas/results/sacCer3/ERX594019/ERX594019.05_peaks.narrowPeak : No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX594019/ERX594019.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX594019/ERX594019.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX594019/ERX594019.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX594019/ERX594019.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX594019/ERX594019.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX594019/ERX594019.20_peaks.narrowPeak’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX594019/ERX594019.10_model.r’: No such file or directory CompletedMACS2peakCalling rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX594019/ERX594019.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/ERX594019/ERX594019.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。