Job ID = 2007861 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... spots read : 198,133 reads read : 396,266 reads written : 396,266 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:06 198133 reads; of these: 198133 (100.00%) were paired; of these: 35422 (17.88%) aligned concordantly 0 times 151201 (76.31%) aligned concordantly exactly 1 time 11510 (5.81%) aligned concordantly >1 times ---- 35422 pairs aligned concordantly 0 times; of these: 19188 (54.17%) aligned discordantly 1 time ---- 16234 pairs aligned 0 times concordantly or discordantly; of these: 32468 mates make up the pairs; of these: 26269 (80.91%) aligned 0 times 2920 (8.99%) aligned exactly 1 time 3279 (10.10%) aligned >1 times 93.37% overall alignment rate Time searching: 00:00:06 Overall time: 00:00:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 1077 / 180457 = 0.0060 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 16:35:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX462402/ERX462402.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX462402/ERX462402.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX462402/ERX462402.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX462402/ERX462402.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 16:35:43: #1 read tag files... INFO @ Fri, 05 Jul 2019 16:35:43: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 16:35:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX462402/ERX462402.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX462402/ERX462402.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX462402/ERX462402.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX462402/ERX462402.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 16:35:44: #1 read tag files... INFO @ Fri, 05 Jul 2019 16:35:44: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 16:35:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX462402/ERX462402.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX462402/ERX462402.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX462402/ERX462402.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX462402/ERX462402.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 16:35:45: #1 read tag files... INFO @ Fri, 05 Jul 2019 16:35:45: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 16:35:45: #1 tag size is determined as 25 bps INFO @ Fri, 05 Jul 2019 16:35:45: #1 tag size = 25 INFO @ Fri, 05 Jul 2019 16:35:45: #1 total tags in treatment: 161728 INFO @ Fri, 05 Jul 2019 16:35:45: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 16:35:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 16:35:45: #1 tags after filtering in treatment: 160286 INFO @ Fri, 05 Jul 2019 16:35:45: #1 Redundant rate of treatment: 0.01 INFO @ Fri, 05 Jul 2019 16:35:45: #1 finished! INFO @ Fri, 05 Jul 2019 16:35:45: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 16:35:45: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 16:35:45: #2 number of paired peaks: 131 WARNING @ Fri, 05 Jul 2019 16:35:45: Fewer paired peaks (131) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 131 pairs to build model! INFO @ Fri, 05 Jul 2019 16:35:45: start model_add_line... INFO @ Fri, 05 Jul 2019 16:35:45: start X-correlation... INFO @ Fri, 05 Jul 2019 16:35:45: end of X-cor INFO @ Fri, 05 Jul 2019 16:35:45: #2 finished! INFO @ Fri, 05 Jul 2019 16:35:45: #2 predicted fragment length is 232 bps INFO @ Fri, 05 Jul 2019 16:35:45: #2 alternative fragment length(s) may be 232 bps INFO @ Fri, 05 Jul 2019 16:35:45: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX462402/ERX462402.05_model.r INFO @ Fri, 05 Jul 2019 16:35:45: #3 Call peaks... INFO @ Fri, 05 Jul 2019 16:35:45: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 05 Jul 2019 16:35:45: #3 Call peaks for each chromosome... INFO @ Fri, 05 Jul 2019 16:35:46: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX462402/ERX462402.05_peaks.xls INFO @ Fri, 05 Jul 2019 16:35:46: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX462402/ERX462402.05_peaks.narrowPeak INFO @ Fri, 05 Jul 2019 16:35:46: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX462402/ERX462402.05_summits.bed INFO @ Fri, 05 Jul 2019 16:35:46: Done! pass1 - making usageList (16 chroms): 1 millis pass2 - checking and writing primary data (230 records, 4 fields): 3 millis INFO @ Fri, 05 Jul 2019 16:35:46: #1 tag size is determined as 25 bps INFO @ Fri, 05 Jul 2019 16:35:46: #1 tag size = 25 INFO @ Fri, 05 Jul 2019 16:35:46: #1 total tags in treatment: 161728 INFO @ Fri, 05 Jul 2019 16:35:46: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 16:35:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 16:35:46: #1 tags after filtering in treatment: 160286 INFO @ Fri, 05 Jul 2019 16:35:46: #1 Redundant rate of treatment: 0.01 INFO @ Fri, 05 Jul 2019 16:35:46: #1 finished! INFO @ Fri, 05 Jul 2019 16:35:46: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 16:35:46: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 16:35:46: #2 number of paired peaks: 131 WARNING @ Fri, 05 Jul 2019 16:35:46: Fewer paired peaks (131) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 131 pairs to build model! INFO @ Fri, 05 Jul 2019 16:35:46: start model_add_line... INFO @ Fri, 05 Jul 2019 16:35:46: start X-correlation... INFO @ Fri, 05 Jul 2019 16:35:46: end of X-cor INFO @ Fri, 05 Jul 2019 16:35:46: #2 finished! INFO @ Fri, 05 Jul 2019 16:35:46: #2 predicted fragment length is 232 bps INFO @ Fri, 05 Jul 2019 16:35:46: #2 alternative fragment length(s) may be 232 bps INFO @ Fri, 05 Jul 2019 16:35:46: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX462402/ERX462402.10_model.r INFO @ Fri, 05 Jul 2019 16:35:46: #3 Call peaks... INFO @ Fri, 05 Jul 2019 16:35:46: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 05 Jul 2019 16:35:47: #3 Call peaks for each chromosome... INFO @ Fri, 05 Jul 2019 16:35:47: #1 tag size is determined as 25 bps INFO @ Fri, 05 Jul 2019 16:35:47: #1 tag size = 25 INFO @ Fri, 05 Jul 2019 16:35:47: #1 total tags in treatment: 161728 INFO @ Fri, 05 Jul 2019 16:35:47: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 16:35:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 16:35:47: #1 tags after filtering in treatment: 160286 INFO @ Fri, 05 Jul 2019 16:35:47: #1 Redundant rate of treatment: 0.01 INFO @ Fri, 05 Jul 2019 16:35:47: #1 finished! INFO @ Fri, 05 Jul 2019 16:35:47: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 16:35:47: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 16:35:47: #2 number of paired peaks: 131 WARNING @ Fri, 05 Jul 2019 16:35:47: Fewer paired peaks (131) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 131 pairs to build model! INFO @ Fri, 05 Jul 2019 16:35:47: start model_add_line... INFO @ Fri, 05 Jul 2019 16:35:47: start X-correlation... INFO @ Fri, 05 Jul 2019 16:35:47: end of X-cor INFO @ Fri, 05 Jul 2019 16:35:47: #2 finished! INFO @ Fri, 05 Jul 2019 16:35:47: #2 predicted fragment length is 232 bps INFO @ Fri, 05 Jul 2019 16:35:47: #2 alternative fragment length(s) may be 232 bps INFO @ Fri, 05 Jul 2019 16:35:47: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX462402/ERX462402.20_model.r INFO @ Fri, 05 Jul 2019 16:35:47: #3 Call peaks... INFO @ Fri, 05 Jul 2019 16:35:47: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 05 Jul 2019 16:35:47: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX462402/ERX462402.10_peaks.xls INFO @ Fri, 05 Jul 2019 16:35:47: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX462402/ERX462402.10_peaks.narrowPeak INFO @ Fri, 05 Jul 2019 16:35:47: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX462402/ERX462402.10_summits.bed INFO @ Fri, 05 Jul 2019 16:35:47: Done! pass1 - making usageList (12 chroms): 1 millis pass2 - checking and writing primary data (26 records, 4 fields): 2 millis INFO @ Fri, 05 Jul 2019 16:35:47: #3 Call peaks for each chromosome... INFO @ Fri, 05 Jul 2019 16:35:48: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX462402/ERX462402.20_peaks.xls INFO @ Fri, 05 Jul 2019 16:35:48: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX462402/ERX462402.20_peaks.narrowPeak INFO @ Fri, 05 Jul 2019 16:35:48: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX462402/ERX462402.20_summits.bed INFO @ Fri, 05 Jul 2019 16:35:48: Done! pass1 - making usageList (2 chroms): 0 millis pass2 - checking and writing primary data (2 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 CompletedMACS2peakCalling CompletedMACS2peakCalling