Job ID = 10223815 SRX = ERX4439575 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-10-15T23:12:15 prefetch.2.10.7: 1) Downloading 'ERR4501504'... 2020-10-15T23:12:15 prefetch.2.10.7: Downloading via HTTPS... 2020-10-15T23:12:48 prefetch.2.10.7: HTTPS download succeed 2020-10-15T23:12:48 prefetch.2.10.7: 'ERR4501504' is valid 2020-10-15T23:12:48 prefetch.2.10.7: 1) 'ERR4501504' was downloaded successfully 2020-10-15T23:13:16 prefetch.2.10.7: 'ERR4501504' has 5 unresolved dependencies 2020-10-15T23:13:16 prefetch.2.10.7: 2) Downloading 'ncbi-acc:NC_001136.8?vdb-ctx=refseq'... 2020-10-15T23:13:16 prefetch.2.10.7: Downloading via HTTPS... 2020-10-15T23:13:29 prefetch.2.10.7: HTTPS download succeed 2020-10-15T23:13:29 prefetch.2.10.7: 2) 'ncbi-acc:NC_001136.8?vdb-ctx=refseq' was downloaded successfully 2020-10-15T23:13:29 prefetch.2.10.7: 3) Downloading 'ncbi-acc:NC_001139.7?vdb-ctx=refseq'... 2020-10-15T23:13:29 prefetch.2.10.7: Downloading via HTTPS... 2020-10-15T23:13:41 prefetch.2.10.7: HTTPS download succeed 2020-10-15T23:13:41 prefetch.2.10.7: 3) 'ncbi-acc:NC_001139.7?vdb-ctx=refseq' was downloaded successfully 2020-10-15T23:13:41 prefetch.2.10.7: 4) Downloading 'ncbi-acc:NC_001144.4?vdb-ctx=refseq'... 2020-10-15T23:13:41 prefetch.2.10.7: Downloading via HTTPS... 2020-10-15T23:13:53 prefetch.2.10.7: HTTPS download succeed 2020-10-15T23:13:53 prefetch.2.10.7: 4) 'ncbi-acc:NC_001144.4?vdb-ctx=refseq' was downloaded successfully 2020-10-15T23:13:53 prefetch.2.10.7: 5) Downloading 'ncbi-acc:NC_001147.5?vdb-ctx=refseq'... 2020-10-15T23:13:53 prefetch.2.10.7: Downloading via HTTPS... 2020-10-15T23:14:04 prefetch.2.10.7: HTTPS download succeed 2020-10-15T23:14:04 prefetch.2.10.7: 5) 'ncbi-acc:NC_001147.5?vdb-ctx=refseq' was downloaded successfully 2020-10-15T23:14:04 prefetch.2.10.7: 6) Downloading 'ncbi-acc:NC_001224.1?vdb-ctx=refseq'... 2020-10-15T23:14:05 prefetch.2.10.7: Downloading via HTTPS... 2020-10-15T23:14:15 prefetch.2.10.7: HTTPS download succeed 2020-10-15T23:14:15 prefetch.2.10.7: 6) 'ncbi-acc:NC_001224.1?vdb-ctx=refseq' was downloaded successfully Read 3240339 spots for ERR4501504/ERR4501504.sra Written 3240339 spots for ERR4501504/ERR4501504.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:26 3240339 reads; of these: 3240339 (100.00%) were paired; of these: 902198 (27.84%) aligned concordantly 0 times 2052823 (63.35%) aligned concordantly exactly 1 time 285318 (8.81%) aligned concordantly >1 times ---- 902198 pairs aligned concordantly 0 times; of these: 416800 (46.20%) aligned discordantly 1 time ---- 485398 pairs aligned 0 times concordantly or discordantly; of these: 970796 mates make up the pairs; of these: 784530 (80.81%) aligned 0 times 57435 (5.92%) aligned exactly 1 time 128831 (13.27%) aligned >1 times 87.89% overall alignment rate Time searching: 00:02:26 Overall time: 00:02:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 46579 / 2727046 = 0.0171 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 08:20:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX4439575/ERX4439575.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX4439575/ERX4439575.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX4439575/ERX4439575.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX4439575/ERX4439575.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 08:20:08: #1 read tag files... INFO @ Fri, 16 Oct 2020 08:20:08: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 08:20:14: 1000000 INFO @ Fri, 16 Oct 2020 08:20:19: 2000000 INFO @ Fri, 16 Oct 2020 08:20:24: 3000000 INFO @ Fri, 16 Oct 2020 08:20:29: 4000000 INFO @ Fri, 16 Oct 2020 08:20:35: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 08:20:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX4439575/ERX4439575.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX4439575/ERX4439575.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX4439575/ERX4439575.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX4439575/ERX4439575.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 08:20:38: #1 read tag files... INFO @ Fri, 16 Oct 2020 08:20:38: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 08:20:39: #1 tag size is determined as 100 bps INFO @ Fri, 16 Oct 2020 08:20:39: #1 tag size = 100 INFO @ Fri, 16 Oct 2020 08:20:39: #1 total tags in treatment: 2302497 INFO @ Fri, 16 Oct 2020 08:20:39: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 08:20:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 08:20:39: #1 tags after filtering in treatment: 1899863 INFO @ Fri, 16 Oct 2020 08:20:39: #1 Redundant rate of treatment: 0.17 INFO @ Fri, 16 Oct 2020 08:20:39: #1 finished! INFO @ Fri, 16 Oct 2020 08:20:39: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 08:20:39: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 08:20:39: #2 number of paired peaks: 124 WARNING @ Fri, 16 Oct 2020 08:20:39: Fewer paired peaks (124) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 124 pairs to build model! INFO @ Fri, 16 Oct 2020 08:20:39: start model_add_line... INFO @ Fri, 16 Oct 2020 08:20:39: start X-correlation... INFO @ Fri, 16 Oct 2020 08:20:39: end of X-cor INFO @ Fri, 16 Oct 2020 08:20:39: #2 finished! INFO @ Fri, 16 Oct 2020 08:20:39: #2 predicted fragment length is 183 bps INFO @ Fri, 16 Oct 2020 08:20:39: #2 alternative fragment length(s) may be 183 bps INFO @ Fri, 16 Oct 2020 08:20:39: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX4439575/ERX4439575.05_model.r WARNING @ Fri, 16 Oct 2020 08:20:39: #2 Since the d (183) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 16 Oct 2020 08:20:39: #2 You may need to consider one of the other alternative d(s): 183 WARNING @ Fri, 16 Oct 2020 08:20:39: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 16 Oct 2020 08:20:39: #3 Call peaks... INFO @ Fri, 16 Oct 2020 08:20:39: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 16 Oct 2020 08:20:44: #3 Call peaks for each chromosome... INFO @ Fri, 16 Oct 2020 08:20:45: 1000000 INFO @ Fri, 16 Oct 2020 08:20:45: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX4439575/ERX4439575.05_peaks.xls INFO @ Fri, 16 Oct 2020 08:20:45: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX4439575/ERX4439575.05_peaks.narrowPeak INFO @ Fri, 16 Oct 2020 08:20:45: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX4439575/ERX4439575.05_summits.bed INFO @ Fri, 16 Oct 2020 08:20:45: Done! pass1 - making usageList (17 chroms): 1 millis pass2 - checking and writing primary data (936 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Fri, 16 Oct 2020 08:20:51: 2000000 INFO @ Fri, 16 Oct 2020 08:20:56: 3000000 INFO @ Fri, 16 Oct 2020 08:21:01: 4000000 INFO @ Fri, 16 Oct 2020 08:21:07: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 08:21:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX4439575/ERX4439575.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX4439575/ERX4439575.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX4439575/ERX4439575.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX4439575/ERX4439575.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 08:21:08: #1 read tag files... INFO @ Fri, 16 Oct 2020 08:21:08: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 08:21:10: #1 tag size is determined as 100 bps INFO @ Fri, 16 Oct 2020 08:21:10: #1 tag size = 100 INFO @ Fri, 16 Oct 2020 08:21:10: #1 total tags in treatment: 2302497 INFO @ Fri, 16 Oct 2020 08:21:10: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 08:21:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 08:21:10: #1 tags after filtering in treatment: 1899863 INFO @ Fri, 16 Oct 2020 08:21:10: #1 Redundant rate of treatment: 0.17 INFO @ Fri, 16 Oct 2020 08:21:10: #1 finished! INFO @ Fri, 16 Oct 2020 08:21:10: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 08:21:10: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 08:21:11: #2 number of paired peaks: 124 WARNING @ Fri, 16 Oct 2020 08:21:11: Fewer paired peaks (124) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 124 pairs to build model! INFO @ Fri, 16 Oct 2020 08:21:11: start model_add_line... INFO @ Fri, 16 Oct 2020 08:21:11: start X-correlation... INFO @ Fri, 16 Oct 2020 08:21:11: end of X-cor INFO @ Fri, 16 Oct 2020 08:21:11: #2 finished! INFO @ Fri, 16 Oct 2020 08:21:11: #2 predicted fragment length is 183 bps INFO @ Fri, 16 Oct 2020 08:21:11: #2 alternative fragment length(s) may be 183 bps INFO @ Fri, 16 Oct 2020 08:21:11: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX4439575/ERX4439575.10_model.r WARNING @ Fri, 16 Oct 2020 08:21:11: #2 Since the d (183) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 16 Oct 2020 08:21:11: #2 You may need to consider one of the other alternative d(s): 183 WARNING @ Fri, 16 Oct 2020 08:21:11: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 16 Oct 2020 08:21:11: #3 Call peaks... INFO @ Fri, 16 Oct 2020 08:21:11: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 16 Oct 2020 08:21:14: 1000000 INFO @ Fri, 16 Oct 2020 08:21:15: #3 Call peaks for each chromosome... INFO @ Fri, 16 Oct 2020 08:21:17: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX4439575/ERX4439575.10_peaks.xls INFO @ Fri, 16 Oct 2020 08:21:17: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX4439575/ERX4439575.10_peaks.narrowPeak INFO @ Fri, 16 Oct 2020 08:21:17: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX4439575/ERX4439575.10_summits.bed INFO @ Fri, 16 Oct 2020 08:21:17: Done! pass1 - making usageList (17 chroms): 1 millis pass2 - checking and writing primary data (760 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Fri, 16 Oct 2020 08:21:19: 2000000 INFO @ Fri, 16 Oct 2020 08:21:24: 3000000 INFO @ Fri, 16 Oct 2020 08:21:30: 4000000 INFO @ Fri, 16 Oct 2020 08:21:35: 5000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 16 Oct 2020 08:21:39: #1 tag size is determined as 100 bps INFO @ Fri, 16 Oct 2020 08:21:39: #1 tag size = 100 INFO @ Fri, 16 Oct 2020 08:21:39: #1 total tags in treatment: 2302497 INFO @ Fri, 16 Oct 2020 08:21:39: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 08:21:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 08:21:39: #1 tags after filtering in treatment: 1899863 INFO @ Fri, 16 Oct 2020 08:21:39: #1 Redundant rate of treatment: 0.17 INFO @ Fri, 16 Oct 2020 08:21:39: #1 finished! INFO @ Fri, 16 Oct 2020 08:21:39: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 08:21:39: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 08:21:39: #2 number of paired peaks: 124 WARNING @ Fri, 16 Oct 2020 08:21:39: Fewer paired peaks (124) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 124 pairs to build model! INFO @ Fri, 16 Oct 2020 08:21:39: start model_add_line... INFO @ Fri, 16 Oct 2020 08:21:39: start X-correlation... INFO @ Fri, 16 Oct 2020 08:21:39: end of X-cor INFO @ Fri, 16 Oct 2020 08:21:39: #2 finished! INFO @ Fri, 16 Oct 2020 08:21:39: #2 predicted fragment length is 183 bps INFO @ Fri, 16 Oct 2020 08:21:39: #2 alternative fragment length(s) may be 183 bps INFO @ Fri, 16 Oct 2020 08:21:39: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX4439575/ERX4439575.20_model.r WARNING @ Fri, 16 Oct 2020 08:21:39: #2 Since the d (183) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 16 Oct 2020 08:21:39: #2 You may need to consider one of the other alternative d(s): 183 WARNING @ Fri, 16 Oct 2020 08:21:39: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 16 Oct 2020 08:21:39: #3 Call peaks... INFO @ Fri, 16 Oct 2020 08:21:39: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Fri, 16 Oct 2020 08:21:44: #3 Call peaks for each chromosome... INFO @ Fri, 16 Oct 2020 08:21:46: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX4439575/ERX4439575.20_peaks.xls INFO @ Fri, 16 Oct 2020 08:21:46: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX4439575/ERX4439575.20_peaks.narrowPeak INFO @ Fri, 16 Oct 2020 08:21:46: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX4439575/ERX4439575.20_summits.bed INFO @ Fri, 16 Oct 2020 08:21:46: Done! pass1 - making usageList (17 chroms): 0 millis pass2 - checking and writing primary data (591 records, 4 fields): 3 millis CompletedMACS2peakCalling