Job ID = 2007761 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... spots read : 32,628 reads read : 65,256 reads written : 65,256 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:02 32628 reads; of these: 32628 (100.00%) were paired; of these: 2775 (8.50%) aligned concordantly 0 times 27566 (84.49%) aligned concordantly exactly 1 time 2287 (7.01%) aligned concordantly >1 times ---- 2775 pairs aligned concordantly 0 times; of these: 1254 (45.19%) aligned discordantly 1 time ---- 1521 pairs aligned 0 times concordantly or discordantly; of these: 3042 mates make up the pairs; of these: 2140 (70.35%) aligned 0 times 645 (21.20%) aligned exactly 1 time 257 (8.45%) aligned >1 times 96.72% overall alignment rate Time searching: 00:00:02 Overall time: 00:00:02 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 43 / 30792 = 0.0014 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 16:26:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX433699/ERX433699.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX433699/ERX433699.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX433699/ERX433699.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX433699/ERX433699.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 16:26:36: #1 read tag files... INFO @ Fri, 05 Jul 2019 16:26:36: #1 read treatment tags... BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 05 Jul 2019 16:26:36: #1 tag size is determined as 25 bps INFO @ Fri, 05 Jul 2019 16:26:36: #1 tag size = 25 INFO @ Fri, 05 Jul 2019 16:26:36: #1 total tags in treatment: 29810 INFO @ Fri, 05 Jul 2019 16:26:36: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 16:26:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 16:26:36: #1 tags after filtering in treatment: 29761 INFO @ Fri, 05 Jul 2019 16:26:36: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 16:26:36: #1 finished! INFO @ Fri, 05 Jul 2019 16:26:36: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 16:26:36: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 16:26:36: #2 number of paired peaks: 315 WARNING @ Fri, 05 Jul 2019 16:26:36: Fewer paired peaks (315) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 315 pairs to build model! INFO @ Fri, 05 Jul 2019 16:26:36: start model_add_line... INFO @ Fri, 05 Jul 2019 16:26:36: start X-correlation... INFO @ Fri, 05 Jul 2019 16:26:36: end of X-cor INFO @ Fri, 05 Jul 2019 16:26:36: #2 finished! INFO @ Fri, 05 Jul 2019 16:26:36: #2 predicted fragment length is 229 bps INFO @ Fri, 05 Jul 2019 16:26:36: #2 alternative fragment length(s) may be 48,102,162,194,229,253,293,324,428,489,549,579 bps INFO @ Fri, 05 Jul 2019 16:26:36: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX433699/ERX433699.05_model.r INFO @ Fri, 05 Jul 2019 16:26:36: #3 Call peaks... INFO @ Fri, 05 Jul 2019 16:26:36: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 05 Jul 2019 16:26:36: #3 Call peaks for each chromosome... INFO @ Fri, 05 Jul 2019 16:26:36: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX433699/ERX433699.05_peaks.xls INFO @ Fri, 05 Jul 2019 16:26:36: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX433699/ERX433699.05_peaks.narrowPeak INFO @ Fri, 05 Jul 2019 16:26:36: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX433699/ERX433699.05_summits.bed INFO @ Fri, 05 Jul 2019 16:26:36: Done! pass1 - making usageList (3 chroms): 3 millis pass2 - checking and writing primary data (5 records, 4 fields): 2 millis INFO @ Fri, 05 Jul 2019 16:26:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX433699/ERX433699.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX433699/ERX433699.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX433699/ERX433699.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX433699/ERX433699.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 16:26:37: #1 read tag files... INFO @ Fri, 05 Jul 2019 16:26:37: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 16:26:37: #1 tag size is determined as 25 bps INFO @ Fri, 05 Jul 2019 16:26:37: #1 tag size = 25 INFO @ Fri, 05 Jul 2019 16:26:37: #1 total tags in treatment: 29810 INFO @ Fri, 05 Jul 2019 16:26:37: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 16:26:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 16:26:37: #1 tags after filtering in treatment: 29761 INFO @ Fri, 05 Jul 2019 16:26:37: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 16:26:37: #1 finished! INFO @ Fri, 05 Jul 2019 16:26:37: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 16:26:37: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 16:26:37: #2 number of paired peaks: 315 WARNING @ Fri, 05 Jul 2019 16:26:37: Fewer paired peaks (315) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 315 pairs to build model! INFO @ Fri, 05 Jul 2019 16:26:37: start model_add_line... INFO @ Fri, 05 Jul 2019 16:26:37: start X-correlation... INFO @ Fri, 05 Jul 2019 16:26:37: end of X-cor INFO @ Fri, 05 Jul 2019 16:26:37: #2 finished! INFO @ Fri, 05 Jul 2019 16:26:37: #2 predicted fragment length is 229 bps INFO @ Fri, 05 Jul 2019 16:26:37: #2 alternative fragment length(s) may be 48,102,162,194,229,253,293,324,428,489,549,579 bps INFO @ Fri, 05 Jul 2019 16:26:37: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX433699/ERX433699.10_model.r INFO @ Fri, 05 Jul 2019 16:26:37: #3 Call peaks... INFO @ Fri, 05 Jul 2019 16:26:37: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 05 Jul 2019 16:26:37: #3 Call peaks for each chromosome... INFO @ Fri, 05 Jul 2019 16:26:37: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX433699/ERX433699.10_peaks.xls INFO @ Fri, 05 Jul 2019 16:26:37: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX433699/ERX433699.10_peaks.narrowPeak INFO @ Fri, 05 Jul 2019 16:26:37: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX433699/ERX433699.10_summits.bed INFO @ Fri, 05 Jul 2019 16:26:37: Done! pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 16:26:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/ERX433699/ERX433699.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/ERX433699/ERX433699.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/ERX433699/ERX433699.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/ERX433699/ERX433699.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 16:26:38: #1 read tag files... INFO @ Fri, 05 Jul 2019 16:26:38: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 16:26:38: #1 tag size is determined as 25 bps INFO @ Fri, 05 Jul 2019 16:26:38: #1 tag size = 25 INFO @ Fri, 05 Jul 2019 16:26:38: #1 total tags in treatment: 29810 INFO @ Fri, 05 Jul 2019 16:26:38: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 16:26:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 16:26:38: #1 tags after filtering in treatment: 29761 INFO @ Fri, 05 Jul 2019 16:26:38: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 16:26:38: #1 finished! INFO @ Fri, 05 Jul 2019 16:26:38: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 16:26:38: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 16:26:38: #2 number of paired peaks: 315 WARNING @ Fri, 05 Jul 2019 16:26:38: Fewer paired peaks (315) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 315 pairs to build model! INFO @ Fri, 05 Jul 2019 16:26:38: start model_add_line... INFO @ Fri, 05 Jul 2019 16:26:38: start X-correlation... INFO @ Fri, 05 Jul 2019 16:26:38: end of X-cor INFO @ Fri, 05 Jul 2019 16:26:38: #2 finished! INFO @ Fri, 05 Jul 2019 16:26:38: #2 predicted fragment length is 229 bps INFO @ Fri, 05 Jul 2019 16:26:38: #2 alternative fragment length(s) may be 48,102,162,194,229,253,293,324,428,489,549,579 bps INFO @ Fri, 05 Jul 2019 16:26:38: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/sacCer3/ERX433699/ERX433699.20_model.r INFO @ Fri, 05 Jul 2019 16:26:38: #3 Call peaks... INFO @ Fri, 05 Jul 2019 16:26:38: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 05 Jul 2019 16:26:38: #3 Call peaks for each chromosome... INFO @ Fri, 05 Jul 2019 16:26:38: #4 Write output xls file... /home/okishinya/chipatlas/results/sacCer3/ERX433699/ERX433699.20_peaks.xls INFO @ Fri, 05 Jul 2019 16:26:38: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/sacCer3/ERX433699/ERX433699.20_peaks.narrowPeak INFO @ Fri, 05 Jul 2019 16:26:38: #4 Write summits bed file... /home/okishinya/chipatlas/results/sacCer3/ERX433699/ERX433699.20_summits.bed INFO @ Fri, 05 Jul 2019 16:26:38: Done! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) BigWig に変換しました。 CompletedMACS2peakCalling CompletedMACS2peakCalling