Job ID = 2640446 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2019-08-24T07:51:01 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T07:51:01 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T07:51:01 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T07:51:39 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - mbedtls_ssl_handshake returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T07:51:39 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - ktls_handshake failed while accessing '130.14.250.26' from '172.19.7.65' 2019-08-24T07:51:39 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - Failed to create TLS stream for 'sra-download.ncbi.nlm.nih.gov' (130.14.250.26) from '172.19.7.65' 2019-08-24T07:52:44 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T07:53:02 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T07:53:02 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T07:53:02 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T07:53:59 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T07:56:12 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T07:57:06 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T07:57:06 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T07:57:49 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T07:58:14 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T07:58:14 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T07:59:18 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T07:59:18 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T08:01:38 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T08:01:39 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T08:04:47 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T08:07:47 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T08:07:48 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T08:08:11 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T08:08:51 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T08:09:23 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T08:10:04 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T08:10:24 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T08:10:24 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T08:12:10 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T08:12:10 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T08:12:34 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T08:13:40 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T08:14:21 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T08:14:21 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T08:15:58 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T08:15:58 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T08:21:09 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-08-24T08:22:32 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 41,175,953 reads read : 82,351,906 reads written : 41,175,953 reads 0-length : 41,175,953 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:01 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:39:25 41175953 reads; of these: 41175953 (100.00%) were unpaired; of these: 3208907 (7.79%) aligned 0 times 27811812 (67.54%) aligned exactly 1 time 10155234 (24.66%) aligned >1 times 92.21% overall alignment rate Time searching: 00:39:28 Overall time: 00:39:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 953 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 1339031 / 37967046 = 0.0353 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Sat, 24 Aug 2019 18:26:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/rn6/SRX4320112/SRX4320112.bam -f BAM -g 2.15e9 -n /home/okishinya/chipatlas/results/rn6/SRX4320112/SRX4320112.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/rn6/SRX4320112/SRX4320112.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/rn6/SRX4320112/SRX4320112.bam'] # control file = None # effective genome size = 2.15e+09 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 18:26:32: #1 read tag files... INFO @ Sat, 24 Aug 2019 18:26:32: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 18:26:40: 1000000 INFO @ Sat, 24 Aug 2019 18:26:49: 2000000 INFO @ Sat, 24 Aug 2019 18:26:57: 3000000 INFO @ Sat, 24 Aug 2019 18:27:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/rn6/SRX4320112/SRX4320112.bam -f BAM -g 2.15e9 -n /home/okishinya/chipatlas/results/rn6/SRX4320112/SRX4320112.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/rn6/SRX4320112/SRX4320112.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/rn6/SRX4320112/SRX4320112.bam'] # control file = None # effective genome size = 2.15e+09 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 18:27:01: #1 read tag files... INFO @ Sat, 24 Aug 2019 18:27:01: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 18:27:06: 4000000 INFO @ Sat, 24 Aug 2019 18:27:10: 1000000 INFO @ Sat, 24 Aug 2019 18:27:14: 5000000 INFO @ Sat, 24 Aug 2019 18:27:18: 2000000 INFO @ Sat, 24 Aug 2019 18:27:23: 6000000 INFO @ Sat, 24 Aug 2019 18:27:27: 3000000 BedGraph に変換中... INFO @ Sat, 24 Aug 2019 18:27:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/rn6/SRX4320112/SRX4320112.bam -f BAM -g 2.15e9 -n /home/okishinya/chipatlas/results/rn6/SRX4320112/SRX4320112.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/rn6/SRX4320112/SRX4320112.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/rn6/SRX4320112/SRX4320112.bam'] # control file = None # effective genome size = 2.15e+09 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 24 Aug 2019 18:27:31: #1 read tag files... INFO @ Sat, 24 Aug 2019 18:27:31: #1 read treatment tags... INFO @ Sat, 24 Aug 2019 18:27:31: 7000000 INFO @ Sat, 24 Aug 2019 18:27:35: 4000000 INFO @ Sat, 24 Aug 2019 18:27:39: 1000000 INFO @ Sat, 24 Aug 2019 18:27:40: 8000000 INFO @ Sat, 24 Aug 2019 18:27:44: 5000000 INFO @ Sat, 24 Aug 2019 18:27:47: 2000000 INFO @ Sat, 24 Aug 2019 18:27:48: 9000000 INFO @ Sat, 24 Aug 2019 18:27:52: 6000000 INFO @ Sat, 24 Aug 2019 18:27:55: 3000000 INFO @ Sat, 24 Aug 2019 18:27:57: 10000000 INFO @ Sat, 24 Aug 2019 18:28:01: 7000000 INFO @ Sat, 24 Aug 2019 18:28:02: 4000000 INFO @ Sat, 24 Aug 2019 18:28:05: 11000000 INFO @ Sat, 24 Aug 2019 18:28:09: 8000000 INFO @ Sat, 24 Aug 2019 18:28:10: 5000000 INFO @ Sat, 24 Aug 2019 18:28:14: 12000000 INFO @ Sat, 24 Aug 2019 18:28:18: 9000000 INFO @ Sat, 24 Aug 2019 18:28:18: 6000000 INFO @ Sat, 24 Aug 2019 18:28:22: 13000000 INFO @ Sat, 24 Aug 2019 18:28:26: 7000000 INFO @ Sat, 24 Aug 2019 18:28:26: 10000000 INFO @ Sat, 24 Aug 2019 18:28:31: 14000000 INFO @ Sat, 24 Aug 2019 18:28:34: 8000000 INFO @ Sat, 24 Aug 2019 18:28:35: 11000000 INFO @ Sat, 24 Aug 2019 18:28:39: 15000000 INFO @ Sat, 24 Aug 2019 18:28:41: 9000000 INFO @ Sat, 24 Aug 2019 18:28:43: 12000000 INFO @ Sat, 24 Aug 2019 18:28:48: 16000000 INFO @ Sat, 24 Aug 2019 18:28:49: 10000000 INFO @ Sat, 24 Aug 2019 18:28:52: 13000000 INFO @ Sat, 24 Aug 2019 18:28:56: 17000000 INFO @ Sat, 24 Aug 2019 18:28:57: 11000000 INFO @ Sat, 24 Aug 2019 18:29:00: 14000000 INFO @ Sat, 24 Aug 2019 18:29:04: 18000000 INFO @ Sat, 24 Aug 2019 18:29:05: 12000000 INFO @ Sat, 24 Aug 2019 18:29:08: 15000000 INFO @ Sat, 24 Aug 2019 18:29:12: 13000000 INFO @ Sat, 24 Aug 2019 18:29:13: 19000000 INFO @ Sat, 24 Aug 2019 18:29:17: 16000000 INFO @ Sat, 24 Aug 2019 18:29:20: 14000000 INFO @ Sat, 24 Aug 2019 18:29:21: 20000000 INFO @ Sat, 24 Aug 2019 18:29:25: 17000000 INFO @ Sat, 24 Aug 2019 18:29:28: 15000000 INFO @ Sat, 24 Aug 2019 18:29:30: 21000000 INFO @ Sat, 24 Aug 2019 18:29:34: 18000000 INFO @ Sat, 24 Aug 2019 18:29:35: 16000000 INFO @ Sat, 24 Aug 2019 18:29:38: 22000000 INFO @ Sat, 24 Aug 2019 18:29:42: 19000000 INFO @ Sat, 24 Aug 2019 18:29:43: 17000000 INFO @ Sat, 24 Aug 2019 18:29:47: 23000000 INFO @ Sat, 24 Aug 2019 18:29:51: 20000000 INFO @ Sat, 24 Aug 2019 18:29:51: 18000000 INFO @ Sat, 24 Aug 2019 18:29:55: 24000000 INFO @ Sat, 24 Aug 2019 18:29:59: 19000000 INFO @ Sat, 24 Aug 2019 18:29:59: 21000000 INFO @ Sat, 24 Aug 2019 18:30:04: 25000000 INFO @ Sat, 24 Aug 2019 18:30:06: 20000000 INFO @ Sat, 24 Aug 2019 18:30:07: 22000000 INFO @ Sat, 24 Aug 2019 18:30:12: 26000000 INFO @ Sat, 24 Aug 2019 18:30:14: 21000000 INFO @ Sat, 24 Aug 2019 18:30:16: 23000000 INFO @ Sat, 24 Aug 2019 18:30:20: 27000000 INFO @ Sat, 24 Aug 2019 18:30:22: 22000000 INFO @ Sat, 24 Aug 2019 18:30:24: 24000000 INFO @ Sat, 24 Aug 2019 18:30:29: 28000000 INFO @ Sat, 24 Aug 2019 18:30:30: 23000000 INFO @ Sat, 24 Aug 2019 18:30:33: 25000000 INFO @ Sat, 24 Aug 2019 18:30:37: 29000000 INFO @ Sat, 24 Aug 2019 18:30:37: 24000000 INFO @ Sat, 24 Aug 2019 18:30:41: 26000000 INFO @ Sat, 24 Aug 2019 18:30:45: 25000000 INFO @ Sat, 24 Aug 2019 18:30:46: 30000000 INFO @ Sat, 24 Aug 2019 18:30:49: 27000000 INFO @ Sat, 24 Aug 2019 18:30:53: 26000000 INFO @ Sat, 24 Aug 2019 18:30:54: 31000000 INFO @ Sat, 24 Aug 2019 18:30:58: 28000000 INFO @ Sat, 24 Aug 2019 18:31:00: 27000000 INFO @ Sat, 24 Aug 2019 18:31:03: 32000000 INFO @ Sat, 24 Aug 2019 18:31:06: 29000000 INFO @ Sat, 24 Aug 2019 18:31:08: 28000000 INFO @ Sat, 24 Aug 2019 18:31:11: 33000000 INFO @ Sat, 24 Aug 2019 18:31:15: 30000000 INFO @ Sat, 24 Aug 2019 18:31:16: 29000000 INFO @ Sat, 24 Aug 2019 18:31:19: 34000000 INFO @ Sat, 24 Aug 2019 18:31:23: 31000000 INFO @ Sat, 24 Aug 2019 18:31:24: 30000000 INFO @ Sat, 24 Aug 2019 18:31:28: 35000000 INFO @ Sat, 24 Aug 2019 18:31:31: 31000000 INFO @ Sat, 24 Aug 2019 18:31:31: 32000000 INFO @ Sat, 24 Aug 2019 18:31:37: 36000000 INFO @ Sat, 24 Aug 2019 18:31:39: 32000000 INFO @ Sat, 24 Aug 2019 18:31:40: 33000000 INFO @ Sat, 24 Aug 2019 18:31:42: #1 tag size is determined as 75 bps INFO @ Sat, 24 Aug 2019 18:31:42: #1 tag size = 75 INFO @ Sat, 24 Aug 2019 18:31:42: #1 total tags in treatment: 36628015 INFO @ Sat, 24 Aug 2019 18:31:42: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 18:31:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 18:31:43: #1 tags after filtering in treatment: 36627949 INFO @ Sat, 24 Aug 2019 18:31:43: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 18:31:43: #1 finished! INFO @ Sat, 24 Aug 2019 18:31:43: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 18:31:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 18:31:47: 33000000 INFO @ Sat, 24 Aug 2019 18:31:47: #2 number of paired peaks: 5341 INFO @ Sat, 24 Aug 2019 18:31:47: start model_add_line... INFO @ Sat, 24 Aug 2019 18:31:48: start X-correlation... INFO @ Sat, 24 Aug 2019 18:31:48: end of X-cor INFO @ Sat, 24 Aug 2019 18:31:48: #2 finished! INFO @ Sat, 24 Aug 2019 18:31:48: #2 predicted fragment length is 76 bps INFO @ Sat, 24 Aug 2019 18:31:48: #2 alternative fragment length(s) may be 76,548 bps INFO @ Sat, 24 Aug 2019 18:31:48: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/rn6/SRX4320112/SRX4320112.05_model.r WARNING @ Sat, 24 Aug 2019 18:31:48: #2 Since the d (76) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 24 Aug 2019 18:31:48: #2 You may need to consider one of the other alternative d(s): 76,548 WARNING @ Sat, 24 Aug 2019 18:31:48: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 24 Aug 2019 18:31:48: #3 Call peaks... INFO @ Sat, 24 Aug 2019 18:31:48: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 24 Aug 2019 18:31:48: 34000000 INFO @ Sat, 24 Aug 2019 18:31:55: 34000000 INFO @ Sat, 24 Aug 2019 18:31:57: 35000000 INFO @ Sat, 24 Aug 2019 18:32:02: 35000000 INFO @ Sat, 24 Aug 2019 18:32:05: 36000000 INFO @ Sat, 24 Aug 2019 18:32:10: 36000000 INFO @ Sat, 24 Aug 2019 18:32:11: #1 tag size is determined as 75 bps INFO @ Sat, 24 Aug 2019 18:32:11: #1 tag size = 75 INFO @ Sat, 24 Aug 2019 18:32:11: #1 total tags in treatment: 36628015 INFO @ Sat, 24 Aug 2019 18:32:11: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 18:32:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 18:32:12: #1 tags after filtering in treatment: 36627949 INFO @ Sat, 24 Aug 2019 18:32:12: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 18:32:12: #1 finished! INFO @ Sat, 24 Aug 2019 18:32:12: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 18:32:12: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 18:32:15: #1 tag size is determined as 75 bps INFO @ Sat, 24 Aug 2019 18:32:15: #1 tag size = 75 INFO @ Sat, 24 Aug 2019 18:32:15: #1 total tags in treatment: 36628015 INFO @ Sat, 24 Aug 2019 18:32:15: #1 user defined the maximum tags... INFO @ Sat, 24 Aug 2019 18:32:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 24 Aug 2019 18:32:16: #2 number of paired peaks: 5341 INFO @ Sat, 24 Aug 2019 18:32:16: start model_add_line... INFO @ Sat, 24 Aug 2019 18:32:16: start X-correlation... INFO @ Sat, 24 Aug 2019 18:32:16: end of X-cor INFO @ Sat, 24 Aug 2019 18:32:16: #2 finished! INFO @ Sat, 24 Aug 2019 18:32:16: #2 predicted fragment length is 76 bps INFO @ Sat, 24 Aug 2019 18:32:16: #2 alternative fragment length(s) may be 76,548 bps INFO @ Sat, 24 Aug 2019 18:32:16: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/rn6/SRX4320112/SRX4320112.10_model.r WARNING @ Sat, 24 Aug 2019 18:32:16: #2 Since the d (76) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 24 Aug 2019 18:32:16: #2 You may need to consider one of the other alternative d(s): 76,548 WARNING @ Sat, 24 Aug 2019 18:32:16: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 24 Aug 2019 18:32:16: #3 Call peaks... INFO @ Sat, 24 Aug 2019 18:32:16: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 24 Aug 2019 18:32:16: #1 tags after filtering in treatment: 36627949 INFO @ Sat, 24 Aug 2019 18:32:16: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 24 Aug 2019 18:32:16: #1 finished! INFO @ Sat, 24 Aug 2019 18:32:16: #2 Build Peak Model... INFO @ Sat, 24 Aug 2019 18:32:16: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 24 Aug 2019 18:32:20: #2 number of paired peaks: 5341 INFO @ Sat, 24 Aug 2019 18:32:20: start model_add_line... INFO @ Sat, 24 Aug 2019 18:32:21: start X-correlation... INFO @ Sat, 24 Aug 2019 18:32:21: end of X-cor INFO @ Sat, 24 Aug 2019 18:32:21: #2 finished! INFO @ Sat, 24 Aug 2019 18:32:21: #2 predicted fragment length is 76 bps INFO @ Sat, 24 Aug 2019 18:32:21: #2 alternative fragment length(s) may be 76,548 bps INFO @ Sat, 24 Aug 2019 18:32:21: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/rn6/SRX4320112/SRX4320112.20_model.r WARNING @ Sat, 24 Aug 2019 18:32:21: #2 Since the d (76) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 24 Aug 2019 18:32:21: #2 You may need to consider one of the other alternative d(s): 76,548 WARNING @ Sat, 24 Aug 2019 18:32:21: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 24 Aug 2019 18:32:21: #3 Call peaks... INFO @ Sat, 24 Aug 2019 18:32:21: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 24 Aug 2019 18:33:42: #3 Call peaks for each chromosome... INFO @ Sat, 24 Aug 2019 18:34:10: #3 Call peaks for each chromosome... INFO @ Sat, 24 Aug 2019 18:34:15: #3 Call peaks for each chromosome... INFO @ Sat, 24 Aug 2019 18:34:38: #4 Write output xls file... /home/okishinya/chipatlas/results/rn6/SRX4320112/SRX4320112.05_peaks.xls INFO @ Sat, 24 Aug 2019 18:34:38: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/rn6/SRX4320112/SRX4320112.05_peaks.narrowPeak INFO @ Sat, 24 Aug 2019 18:34:38: #4 Write summits bed file... /home/okishinya/chipatlas/results/rn6/SRX4320112/SRX4320112.05_summits.bed INFO @ Sat, 24 Aug 2019 18:34:38: Done! pass1 - making usageList (83 chroms): 3 millis pass2 - checking and writing primary data (4149 records, 4 fields): 16 millis CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 18:35:07: #4 Write output xls file... /home/okishinya/chipatlas/results/rn6/SRX4320112/SRX4320112.10_peaks.xls INFO @ Sat, 24 Aug 2019 18:35:07: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/rn6/SRX4320112/SRX4320112.10_peaks.narrowPeak INFO @ Sat, 24 Aug 2019 18:35:07: #4 Write summits bed file... /home/okishinya/chipatlas/results/rn6/SRX4320112/SRX4320112.10_summits.bed INFO @ Sat, 24 Aug 2019 18:35:07: Done! pass1 - making usageList (63 chroms): 2 millis pass2 - checking and writing primary data (1607 records, 4 fields): 8 millis CompletedMACS2peakCalling INFO @ Sat, 24 Aug 2019 18:35:12: #4 Write output xls file... /home/okishinya/chipatlas/results/rn6/SRX4320112/SRX4320112.20_peaks.xls INFO @ Sat, 24 Aug 2019 18:35:12: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/rn6/SRX4320112/SRX4320112.20_peaks.narrowPeak INFO @ Sat, 24 Aug 2019 18:35:12: #4 Write summits bed file... /home/okishinya/chipatlas/results/rn6/SRX4320112/SRX4320112.20_summits.bed INFO @ Sat, 24 Aug 2019 18:35:12: Done! pass1 - making usageList (42 chroms): 2 millis pass2 - checking and writing primary data (626 records, 4 fields): 6 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。