Job ID = 10196052 sra ファイルのダウンロード中... Completed: 2088109K bytes transferred in 21 seconds (803348K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 64000000 spots for /home/okishinya/chipatlas/results/rn6/SRX1776226/SRR3547946.sra Written 64000000 spots total rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:01 Time loading forward index: 00:00:02 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:09:39 64000000 reads; of these: 64000000 (100.00%) were unpaired; of these: 63967339 (99.95%) aligned 0 times 23106 (0.04%) aligned exactly 1 time 9555 (0.01%) aligned >1 times 0.05% overall alignment rate Time searching: 00:09:43 Overall time: 00:09:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 953 sequences. [bam_rmdupse_core] 2305 / 32661 = 0.0706 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 11 Nov 2017 08:51:08: # Command line: callpeak -t SRX1776226.bam -f BAM -g 2.15e9 -n SRX1776226.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1776226.10 # format = BAM # ChIP-seq file = ['SRX1776226.bam'] # control file = None # effective genome size = 2.15e+09 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Nov 2017 08:51:08: #1 read tag files... INFO @ Sat, 11 Nov 2017 08:51:08: #1 read treatment tags... INFO @ Sat, 11 Nov 2017 08:51:08: # Command line: callpeak -t SRX1776226.bam -f BAM -g 2.15e9 -n SRX1776226.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1776226.20 # format = BAM # ChIP-seq file = ['SRX1776226.bam'] # control file = None # effective genome size = 2.15e+09 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Nov 2017 08:51:08: #1 read tag files... INFO @ Sat, 11 Nov 2017 08:51:08: #1 read treatment tags... INFO @ Sat, 11 Nov 2017 08:51:08: # Command line: callpeak -t SRX1776226.bam -f BAM -g 2.15e9 -n SRX1776226.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1776226.05 # format = BAM # ChIP-seq file = ['SRX1776226.bam'] # control file = None # effective genome size = 2.15e+09 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Nov 2017 08:51:08: #1 read tag files... INFO @ Sat, 11 Nov 2017 08:51:08: #1 read treatment tags... INFO @ Sat, 11 Nov 2017 08:51:08: #1 tag size is determined as 51 bps INFO @ Sat, 11 Nov 2017 08:51:08: #1 tag size = 51 INFO @ Sat, 11 Nov 2017 08:51:08: #1 total tags in treatment: 30356 INFO @ Sat, 11 Nov 2017 08:51:08: #1 user defined the maximum tags... INFO @ Sat, 11 Nov 2017 08:51:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Nov 2017 08:51:08: #1 tag size is determined as 51 bps INFO @ Sat, 11 Nov 2017 08:51:08: #1 tag size = 51 INFO @ Sat, 11 Nov 2017 08:51:08: #1 total tags in treatment: 30356 INFO @ Sat, 11 Nov 2017 08:51:08: #1 user defined the maximum tags... INFO @ Sat, 11 Nov 2017 08:51:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Nov 2017 08:51:08: #1 tag size is determined as 51 bps INFO @ Sat, 11 Nov 2017 08:51:08: #1 tag size = 51 INFO @ Sat, 11 Nov 2017 08:51:08: #1 total tags in treatment: 30356 INFO @ Sat, 11 Nov 2017 08:51:08: #1 user defined the maximum tags... INFO @ Sat, 11 Nov 2017 08:51:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Nov 2017 08:51:08: #1 tags after filtering in treatment: 30270 INFO @ Sat, 11 Nov 2017 08:51:08: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Nov 2017 08:51:08: #1 finished! INFO @ Sat, 11 Nov 2017 08:51:08: #2 Build Peak Model... INFO @ Sat, 11 Nov 2017 08:51:08: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Nov 2017 08:51:08: #1 tags after filtering in treatment: 30270 INFO @ Sat, 11 Nov 2017 08:51:08: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Nov 2017 08:51:08: #1 finished! INFO @ Sat, 11 Nov 2017 08:51:08: #2 Build Peak Model... INFO @ Sat, 11 Nov 2017 08:51:08: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Nov 2017 08:51:08: #2 number of paired peaks: 0 WARNING @ Sat, 11 Nov 2017 08:51:08: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Nov 2017 08:51:08: Process for pairing-model is terminated! INFO @ Sat, 11 Nov 2017 08:51:08: #1 tags after filtering in treatment: 30270 INFO @ Sat, 11 Nov 2017 08:51:08: #2 number of paired peaks: 0 WARNING @ Sat, 11 Nov 2017 08:51:08: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. INFO @ Sat, 11 Nov 2017 08:51:08: #1 Redundant rate of treatment: 0.00 WARNING @ Sat, 11 Nov 2017 08:51:08: Process for pairing-model is terminated! INFO @ Sat, 11 Nov 2017 08:51:08: #1 finished! INFO @ Sat, 11 Nov 2017 08:51:08: #2 Build Peak Model... INFO @ Sat, 11 Nov 2017 08:51:08: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Nov 2017 08:51:08: #2 number of paired peaks: 0 WARNING @ Sat, 11 Nov 2017 08:51:08: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Nov 2017 08:51:08: Process for pairing-model is terminated! cat: cat: SRX1776226.10_peaks.narrowPeakSRX1776226.05_peaks.narrowPeakcat: SRX1776226.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません: そのようなファイルやディレクトリはありません : そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis pass1 - making usageList (0 chroms): 2 millis pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1776226.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1776226.20_*.xls': そのようなファイルやディレクトリはありませんrm: cannot remove `SRX1776226.10_model.r'rm: cannot remove `SRX1776226.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません: そのようなファイルやディレクトリはありません rm: cannot remove `SRX1776226.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1776226.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling CompletedMACS2peakCalling needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1776226.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1776226.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1776226.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。