Job ID = 10223664 SRX = ERX2745138 Genome = rn6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-10-15T20:30:29 prefetch.2.10.7: 1) Downloading 'ERR2731913'... 2020-10-15T20:30:29 prefetch.2.10.7: Downloading via HTTPS... 2020-10-15T20:32:29 prefetch.2.10.7: HTTPS download succeed 2020-10-15T20:32:29 prefetch.2.10.7: 1) 'ERR2731913' was downloaded successfully Read 102392549 spots for ERR2731913/ERR2731913.sra Written 102392549 spots for ERR2731913/ERR2731913.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:02 Time loading forward index: 00:00:02 Time loading mirror index: 00:00:02 Multiseed full-index search: 01:13:58 102392549 reads; of these: 102392549 (100.00%) were unpaired; of these: 2587702 (2.53%) aligned 0 times 70292952 (68.65%) aligned exactly 1 time 29511895 (28.82%) aligned >1 times 97.47% overall alignment rate Time searching: 01:14:05 Overall time: 01:14:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 953 sequences. [bam_sort_core] merging from 44 files... [bam_rmdupse_core] 26424550 / 99804847 = 0.2648 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 07:06:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/rn6/ERX2745138/ERX2745138.bam -f BAM -g 2.15e9 -n /home/okishinya/chipatlas/results/rn6/ERX2745138/ERX2745138.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/rn6/ERX2745138/ERX2745138.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/rn6/ERX2745138/ERX2745138.bam'] # control file = None # effective genome size = 2.15e+09 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 07:06:49: #1 read tag files... INFO @ Fri, 16 Oct 2020 07:06:49: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 07:06:56: 1000000 INFO @ Fri, 16 Oct 2020 07:07:02: 2000000 INFO @ Fri, 16 Oct 2020 07:07:07: 3000000 INFO @ Fri, 16 Oct 2020 07:07:13: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 07:07:18: 5000000 INFO @ Fri, 16 Oct 2020 07:07:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/rn6/ERX2745138/ERX2745138.bam -f BAM -g 2.15e9 -n /home/okishinya/chipatlas/results/rn6/ERX2745138/ERX2745138.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/rn6/ERX2745138/ERX2745138.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/rn6/ERX2745138/ERX2745138.bam'] # control file = None # effective genome size = 2.15e+09 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 07:07:19: #1 read tag files... INFO @ Fri, 16 Oct 2020 07:07:19: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 07:07:25: 6000000 INFO @ Fri, 16 Oct 2020 07:07:26: 1000000 INFO @ Fri, 16 Oct 2020 07:07:31: 7000000 INFO @ Fri, 16 Oct 2020 07:07:32: 2000000 INFO @ Fri, 16 Oct 2020 07:07:37: 8000000 INFO @ Fri, 16 Oct 2020 07:07:38: 3000000 INFO @ Fri, 16 Oct 2020 07:07:44: 9000000 INFO @ Fri, 16 Oct 2020 07:07:44: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 07:07:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/rn6/ERX2745138/ERX2745138.bam -f BAM -g 2.15e9 -n /home/okishinya/chipatlas/results/rn6/ERX2745138/ERX2745138.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/rn6/ERX2745138/ERX2745138.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/rn6/ERX2745138/ERX2745138.bam'] # control file = None # effective genome size = 2.15e+09 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 07:07:49: #1 read tag files... INFO @ Fri, 16 Oct 2020 07:07:49: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 07:07:50: 10000000 INFO @ Fri, 16 Oct 2020 07:07:51: 5000000 INFO @ Fri, 16 Oct 2020 07:07:55: 1000000 INFO @ Fri, 16 Oct 2020 07:07:56: 11000000 INFO @ Fri, 16 Oct 2020 07:07:57: 6000000 INFO @ Fri, 16 Oct 2020 07:08:02: 2000000 INFO @ Fri, 16 Oct 2020 07:08:02: 12000000 INFO @ Fri, 16 Oct 2020 07:08:03: 7000000 INFO @ Fri, 16 Oct 2020 07:08:08: 3000000 INFO @ Fri, 16 Oct 2020 07:08:09: 13000000 INFO @ Fri, 16 Oct 2020 07:08:09: 8000000 INFO @ Fri, 16 Oct 2020 07:08:14: 4000000 INFO @ Fri, 16 Oct 2020 07:08:15: 14000000 INFO @ Fri, 16 Oct 2020 07:08:15: 9000000 INFO @ Fri, 16 Oct 2020 07:08:20: 5000000 INFO @ Fri, 16 Oct 2020 07:08:21: 10000000 INFO @ Fri, 16 Oct 2020 07:08:21: 15000000 INFO @ Fri, 16 Oct 2020 07:08:27: 6000000 INFO @ Fri, 16 Oct 2020 07:08:27: 11000000 INFO @ Fri, 16 Oct 2020 07:08:27: 16000000 INFO @ Fri, 16 Oct 2020 07:08:33: 7000000 INFO @ Fri, 16 Oct 2020 07:08:33: 12000000 INFO @ Fri, 16 Oct 2020 07:08:33: 17000000 INFO @ Fri, 16 Oct 2020 07:08:39: 8000000 INFO @ Fri, 16 Oct 2020 07:08:39: 13000000 INFO @ Fri, 16 Oct 2020 07:08:40: 18000000 INFO @ Fri, 16 Oct 2020 07:08:45: 14000000 INFO @ Fri, 16 Oct 2020 07:08:45: 9000000 INFO @ Fri, 16 Oct 2020 07:08:46: 19000000 INFO @ Fri, 16 Oct 2020 07:08:51: 15000000 INFO @ Fri, 16 Oct 2020 07:08:51: 10000000 INFO @ Fri, 16 Oct 2020 07:08:52: 20000000 INFO @ Fri, 16 Oct 2020 07:08:57: 16000000 INFO @ Fri, 16 Oct 2020 07:08:57: 11000000 INFO @ Fri, 16 Oct 2020 07:08:58: 21000000 INFO @ Fri, 16 Oct 2020 07:09:03: 17000000 INFO @ Fri, 16 Oct 2020 07:09:04: 12000000 INFO @ Fri, 16 Oct 2020 07:09:04: 22000000 INFO @ Fri, 16 Oct 2020 07:09:09: 18000000 INFO @ Fri, 16 Oct 2020 07:09:10: 13000000 INFO @ Fri, 16 Oct 2020 07:09:10: 23000000 INFO @ Fri, 16 Oct 2020 07:09:15: 19000000 INFO @ Fri, 16 Oct 2020 07:09:16: 14000000 INFO @ Fri, 16 Oct 2020 07:09:16: 24000000 INFO @ Fri, 16 Oct 2020 07:09:21: 20000000 INFO @ Fri, 16 Oct 2020 07:09:22: 15000000 INFO @ Fri, 16 Oct 2020 07:09:23: 25000000 INFO @ Fri, 16 Oct 2020 07:09:27: 21000000 INFO @ Fri, 16 Oct 2020 07:09:28: 16000000 INFO @ Fri, 16 Oct 2020 07:09:29: 26000000 INFO @ Fri, 16 Oct 2020 07:09:33: 22000000 INFO @ Fri, 16 Oct 2020 07:09:34: 17000000 INFO @ Fri, 16 Oct 2020 07:09:35: 27000000 INFO @ Fri, 16 Oct 2020 07:09:39: 23000000 INFO @ Fri, 16 Oct 2020 07:09:40: 18000000 INFO @ Fri, 16 Oct 2020 07:09:41: 28000000 INFO @ Fri, 16 Oct 2020 07:09:45: 24000000 INFO @ Fri, 16 Oct 2020 07:09:46: 19000000 INFO @ Fri, 16 Oct 2020 07:09:47: 29000000 INFO @ Fri, 16 Oct 2020 07:09:51: 25000000 INFO @ Fri, 16 Oct 2020 07:09:53: 20000000 INFO @ Fri, 16 Oct 2020 07:09:53: 30000000 INFO @ Fri, 16 Oct 2020 07:09:57: 26000000 INFO @ Fri, 16 Oct 2020 07:09:59: 21000000 INFO @ Fri, 16 Oct 2020 07:09:59: 31000000 INFO @ Fri, 16 Oct 2020 07:10:03: 27000000 INFO @ Fri, 16 Oct 2020 07:10:05: 22000000 INFO @ Fri, 16 Oct 2020 07:10:05: 32000000 INFO @ Fri, 16 Oct 2020 07:10:09: 28000000 INFO @ Fri, 16 Oct 2020 07:10:11: 23000000 INFO @ Fri, 16 Oct 2020 07:10:11: 33000000 INFO @ Fri, 16 Oct 2020 07:10:15: 29000000 INFO @ Fri, 16 Oct 2020 07:10:17: 24000000 INFO @ Fri, 16 Oct 2020 07:10:17: 34000000 INFO @ Fri, 16 Oct 2020 07:10:21: 30000000 INFO @ Fri, 16 Oct 2020 07:10:23: 25000000 INFO @ Fri, 16 Oct 2020 07:10:23: 35000000 INFO @ Fri, 16 Oct 2020 07:10:26: 31000000 INFO @ Fri, 16 Oct 2020 07:10:29: 26000000 INFO @ Fri, 16 Oct 2020 07:10:29: 36000000 INFO @ Fri, 16 Oct 2020 07:10:32: 32000000 INFO @ Fri, 16 Oct 2020 07:10:35: 27000000 INFO @ Fri, 16 Oct 2020 07:10:35: 37000000 INFO @ Fri, 16 Oct 2020 07:10:38: 33000000 INFO @ Fri, 16 Oct 2020 07:10:41: 28000000 INFO @ Fri, 16 Oct 2020 07:10:41: 38000000 INFO @ Fri, 16 Oct 2020 07:10:44: 34000000 INFO @ Fri, 16 Oct 2020 07:10:47: 29000000 INFO @ Fri, 16 Oct 2020 07:10:47: 39000000 INFO @ Fri, 16 Oct 2020 07:10:50: 35000000 INFO @ Fri, 16 Oct 2020 07:10:53: 30000000 INFO @ Fri, 16 Oct 2020 07:10:53: 40000000 INFO @ Fri, 16 Oct 2020 07:10:56: 36000000 INFO @ Fri, 16 Oct 2020 07:10:59: 31000000 INFO @ Fri, 16 Oct 2020 07:10:59: 41000000 INFO @ Fri, 16 Oct 2020 07:11:02: 37000000 INFO @ Fri, 16 Oct 2020 07:11:05: 32000000 INFO @ Fri, 16 Oct 2020 07:11:05: 42000000 INFO @ Fri, 16 Oct 2020 07:11:08: 38000000 INFO @ Fri, 16 Oct 2020 07:11:11: 33000000 INFO @ Fri, 16 Oct 2020 07:11:12: 43000000 INFO @ Fri, 16 Oct 2020 07:11:14: 39000000 INFO @ Fri, 16 Oct 2020 07:11:18: 34000000 INFO @ Fri, 16 Oct 2020 07:11:19: 44000000 INFO @ Fri, 16 Oct 2020 07:11:21: 40000000 INFO @ Fri, 16 Oct 2020 07:11:24: 35000000 INFO @ Fri, 16 Oct 2020 07:11:25: 45000000 INFO @ Fri, 16 Oct 2020 07:11:27: 41000000 INFO @ Fri, 16 Oct 2020 07:11:31: 36000000 INFO @ Fri, 16 Oct 2020 07:11:32: 46000000 INFO @ Fri, 16 Oct 2020 07:11:34: 42000000 INFO @ Fri, 16 Oct 2020 07:11:37: 37000000 INFO @ Fri, 16 Oct 2020 07:11:40: 47000000 INFO @ Fri, 16 Oct 2020 07:11:40: 43000000 INFO @ Fri, 16 Oct 2020 07:11:44: 38000000 INFO @ Fri, 16 Oct 2020 07:11:46: 44000000 INFO @ Fri, 16 Oct 2020 07:11:46: 48000000 INFO @ Fri, 16 Oct 2020 07:11:50: 39000000 INFO @ Fri, 16 Oct 2020 07:11:53: 45000000 INFO @ Fri, 16 Oct 2020 07:11:53: 49000000 INFO @ Fri, 16 Oct 2020 07:11:56: 40000000 INFO @ Fri, 16 Oct 2020 07:11:59: 46000000 INFO @ Fri, 16 Oct 2020 07:12:00: 50000000 INFO @ Fri, 16 Oct 2020 07:12:03: 41000000 INFO @ Fri, 16 Oct 2020 07:12:06: 47000000 INFO @ Fri, 16 Oct 2020 07:12:06: 51000000 INFO @ Fri, 16 Oct 2020 07:12:09: 42000000 INFO @ Fri, 16 Oct 2020 07:12:12: 48000000 INFO @ Fri, 16 Oct 2020 07:12:12: 52000000 INFO @ Fri, 16 Oct 2020 07:12:15: 43000000 INFO @ Fri, 16 Oct 2020 07:12:18: 49000000 INFO @ Fri, 16 Oct 2020 07:12:18: 53000000 INFO @ Fri, 16 Oct 2020 07:12:21: 44000000 INFO @ Fri, 16 Oct 2020 07:12:24: 50000000 INFO @ Fri, 16 Oct 2020 07:12:25: 54000000 INFO @ Fri, 16 Oct 2020 07:12:27: 45000000 INFO @ Fri, 16 Oct 2020 07:12:31: 51000000 INFO @ Fri, 16 Oct 2020 07:12:31: 55000000 INFO @ Fri, 16 Oct 2020 07:12:34: 46000000 INFO @ Fri, 16 Oct 2020 07:12:37: 52000000 INFO @ Fri, 16 Oct 2020 07:12:38: 56000000 INFO @ Fri, 16 Oct 2020 07:12:40: 47000000 INFO @ Fri, 16 Oct 2020 07:12:44: 53000000 INFO @ Fri, 16 Oct 2020 07:12:44: 57000000 INFO @ Fri, 16 Oct 2020 07:12:47: 48000000 INFO @ Fri, 16 Oct 2020 07:12:50: 54000000 INFO @ Fri, 16 Oct 2020 07:12:51: 58000000 INFO @ Fri, 16 Oct 2020 07:12:53: 49000000 INFO @ Fri, 16 Oct 2020 07:12:57: 55000000 INFO @ Fri, 16 Oct 2020 07:12:58: 59000000 INFO @ Fri, 16 Oct 2020 07:13:00: 50000000 INFO @ Fri, 16 Oct 2020 07:13:03: 56000000 INFO @ Fri, 16 Oct 2020 07:13:05: 60000000 INFO @ Fri, 16 Oct 2020 07:13:06: 51000000 INFO @ Fri, 16 Oct 2020 07:13:09: 57000000 INFO @ Fri, 16 Oct 2020 07:13:11: 61000000 INFO @ Fri, 16 Oct 2020 07:13:13: 52000000 INFO @ Fri, 16 Oct 2020 07:13:16: 58000000 INFO @ Fri, 16 Oct 2020 07:13:18: 62000000 INFO @ Fri, 16 Oct 2020 07:13:19: 53000000 INFO @ Fri, 16 Oct 2020 07:13:23: 59000000 INFO @ Fri, 16 Oct 2020 07:13:25: 63000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 16 Oct 2020 07:13:26: 54000000 INFO @ Fri, 16 Oct 2020 07:13:29: 60000000 INFO @ Fri, 16 Oct 2020 07:13:32: 64000000 INFO @ Fri, 16 Oct 2020 07:13:32: 55000000 INFO @ Fri, 16 Oct 2020 07:13:36: 61000000 INFO @ Fri, 16 Oct 2020 07:13:39: 65000000 INFO @ Fri, 16 Oct 2020 07:13:39: 56000000 INFO @ Fri, 16 Oct 2020 07:13:42: 62000000 INFO @ Fri, 16 Oct 2020 07:13:46: 57000000 INFO @ Fri, 16 Oct 2020 07:13:46: 66000000 INFO @ Fri, 16 Oct 2020 07:13:49: 63000000 INFO @ Fri, 16 Oct 2020 07:13:52: 58000000 INFO @ Fri, 16 Oct 2020 07:13:53: 67000000 INFO @ Fri, 16 Oct 2020 07:13:56: 64000000 INFO @ Fri, 16 Oct 2020 07:13:59: 59000000 INFO @ Fri, 16 Oct 2020 07:14:00: 68000000 INFO @ Fri, 16 Oct 2020 07:14:02: 65000000 INFO @ Fri, 16 Oct 2020 07:14:05: 60000000 INFO @ Fri, 16 Oct 2020 07:14:08: 69000000 INFO @ Fri, 16 Oct 2020 07:14:09: 66000000 INFO @ Fri, 16 Oct 2020 07:14:12: 61000000 INFO @ Fri, 16 Oct 2020 07:14:15: 70000000 INFO @ Fri, 16 Oct 2020 07:14:15: 67000000 INFO @ Fri, 16 Oct 2020 07:14:18: 62000000 INFO @ Fri, 16 Oct 2020 07:14:22: 71000000 INFO @ Fri, 16 Oct 2020 07:14:22: 68000000 INFO @ Fri, 16 Oct 2020 07:14:25: 63000000 INFO @ Fri, 16 Oct 2020 07:14:28: 69000000 INFO @ Fri, 16 Oct 2020 07:14:29: 72000000 INFO @ Fri, 16 Oct 2020 07:14:31: 64000000 INFO @ Fri, 16 Oct 2020 07:14:35: 70000000 INFO @ Fri, 16 Oct 2020 07:14:36: 73000000 INFO @ Fri, 16 Oct 2020 07:14:38: 65000000 INFO @ Fri, 16 Oct 2020 07:14:39: #1 tag size is determined as 50 bps INFO @ Fri, 16 Oct 2020 07:14:39: #1 tag size = 50 INFO @ Fri, 16 Oct 2020 07:14:39: #1 total tags in treatment: 73380297 INFO @ Fri, 16 Oct 2020 07:14:39: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 07:14:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 07:14:40: #1 tags after filtering in treatment: 73380276 INFO @ Fri, 16 Oct 2020 07:14:40: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 16 Oct 2020 07:14:40: #1 finished! INFO @ Fri, 16 Oct 2020 07:14:40: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 07:14:40: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 07:14:41: 71000000 INFO @ Fri, 16 Oct 2020 07:14:44: 66000000 INFO @ Fri, 16 Oct 2020 07:14:46: #2 number of paired peaks: 6295 INFO @ Fri, 16 Oct 2020 07:14:46: start model_add_line... INFO @ Fri, 16 Oct 2020 07:14:46: start X-correlation... INFO @ Fri, 16 Oct 2020 07:14:47: end of X-cor INFO @ Fri, 16 Oct 2020 07:14:47: #2 finished! INFO @ Fri, 16 Oct 2020 07:14:47: #2 predicted fragment length is 50 bps INFO @ Fri, 16 Oct 2020 07:14:47: #2 alternative fragment length(s) may be 50 bps INFO @ Fri, 16 Oct 2020 07:14:47: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/rn6/ERX2745138/ERX2745138.05_model.r WARNING @ Fri, 16 Oct 2020 07:14:47: #2 Since the d (50) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 16 Oct 2020 07:14:47: #2 You may need to consider one of the other alternative d(s): 50 WARNING @ Fri, 16 Oct 2020 07:14:47: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 16 Oct 2020 07:14:47: #3 Call peaks... INFO @ Fri, 16 Oct 2020 07:14:47: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 16 Oct 2020 07:14:48: 72000000 INFO @ Fri, 16 Oct 2020 07:14:50: 67000000 INFO @ Fri, 16 Oct 2020 07:14:54: 73000000 INFO @ Fri, 16 Oct 2020 07:14:56: 68000000 INFO @ Fri, 16 Oct 2020 07:14:57: #1 tag size is determined as 50 bps INFO @ Fri, 16 Oct 2020 07:14:57: #1 tag size = 50 INFO @ Fri, 16 Oct 2020 07:14:57: #1 total tags in treatment: 73380297 INFO @ Fri, 16 Oct 2020 07:14:57: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 07:14:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 07:14:58: #1 tags after filtering in treatment: 73380276 INFO @ Fri, 16 Oct 2020 07:14:58: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 16 Oct 2020 07:14:58: #1 finished! INFO @ Fri, 16 Oct 2020 07:14:58: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 07:14:58: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 07:15:02: 69000000 INFO @ Fri, 16 Oct 2020 07:15:03: #2 number of paired peaks: 6295 INFO @ Fri, 16 Oct 2020 07:15:03: start model_add_line... INFO @ Fri, 16 Oct 2020 07:15:04: start X-correlation... INFO @ Fri, 16 Oct 2020 07:15:04: end of X-cor INFO @ Fri, 16 Oct 2020 07:15:04: #2 finished! INFO @ Fri, 16 Oct 2020 07:15:04: #2 predicted fragment length is 50 bps INFO @ Fri, 16 Oct 2020 07:15:04: #2 alternative fragment length(s) may be 50 bps INFO @ Fri, 16 Oct 2020 07:15:04: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/rn6/ERX2745138/ERX2745138.10_model.r WARNING @ Fri, 16 Oct 2020 07:15:04: #2 Since the d (50) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 16 Oct 2020 07:15:04: #2 You may need to consider one of the other alternative d(s): 50 WARNING @ Fri, 16 Oct 2020 07:15:04: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 16 Oct 2020 07:15:04: #3 Call peaks... INFO @ Fri, 16 Oct 2020 07:15:04: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 16 Oct 2020 07:15:08: 70000000 INFO @ Fri, 16 Oct 2020 07:15:14: 71000000 INFO @ Fri, 16 Oct 2020 07:15:20: 72000000 INFO @ Fri, 16 Oct 2020 07:15:25: 73000000 INFO @ Fri, 16 Oct 2020 07:15:28: #1 tag size is determined as 50 bps INFO @ Fri, 16 Oct 2020 07:15:28: #1 tag size = 50 INFO @ Fri, 16 Oct 2020 07:15:28: #1 total tags in treatment: 73380297 INFO @ Fri, 16 Oct 2020 07:15:28: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 07:15:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 07:15:29: #1 tags after filtering in treatment: 73380276 INFO @ Fri, 16 Oct 2020 07:15:29: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 16 Oct 2020 07:15:29: #1 finished! INFO @ Fri, 16 Oct 2020 07:15:29: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 07:15:29: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 07:15:35: #2 number of paired peaks: 6295 INFO @ Fri, 16 Oct 2020 07:15:35: start model_add_line... INFO @ Fri, 16 Oct 2020 07:15:35: start X-correlation... INFO @ Fri, 16 Oct 2020 07:15:35: end of X-cor INFO @ Fri, 16 Oct 2020 07:15:35: #2 finished! INFO @ Fri, 16 Oct 2020 07:15:35: #2 predicted fragment length is 50 bps INFO @ Fri, 16 Oct 2020 07:15:35: #2 alternative fragment length(s) may be 50 bps INFO @ Fri, 16 Oct 2020 07:15:35: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/rn6/ERX2745138/ERX2745138.20_model.r WARNING @ Fri, 16 Oct 2020 07:15:35: #2 Since the d (50) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 16 Oct 2020 07:15:35: #2 You may need to consider one of the other alternative d(s): 50 WARNING @ Fri, 16 Oct 2020 07:15:35: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 16 Oct 2020 07:15:35: #3 Call peaks... INFO @ Fri, 16 Oct 2020 07:15:35: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Fri, 16 Oct 2020 07:17:21: #3 Call peaks for each chromosome... INFO @ Fri, 16 Oct 2020 07:17:49: #3 Call peaks for each chromosome... INFO @ Fri, 16 Oct 2020 07:18:19: #3 Call peaks for each chromosome... INFO @ Fri, 16 Oct 2020 07:18:45: #4 Write output xls file... /home/okishinya/chipatlas/results/rn6/ERX2745138/ERX2745138.05_peaks.xls INFO @ Fri, 16 Oct 2020 07:18:45: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/rn6/ERX2745138/ERX2745138.05_peaks.narrowPeak INFO @ Fri, 16 Oct 2020 07:18:45: #4 Write summits bed file... /home/okishinya/chipatlas/results/rn6/ERX2745138/ERX2745138.05_summits.bed INFO @ Fri, 16 Oct 2020 07:18:45: Done! pass1 - making usageList (75 chroms): 2 millis pass2 - checking and writing primary data (3659 records, 4 fields): 8 millis CompletedMACS2peakCalling INFO @ Fri, 16 Oct 2020 07:19:15: #4 Write output xls file... /home/okishinya/chipatlas/results/rn6/ERX2745138/ERX2745138.10_peaks.xls INFO @ Fri, 16 Oct 2020 07:19:15: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/rn6/ERX2745138/ERX2745138.10_peaks.narrowPeak INFO @ Fri, 16 Oct 2020 07:19:15: #4 Write summits bed file... /home/okishinya/chipatlas/results/rn6/ERX2745138/ERX2745138.10_summits.bed INFO @ Fri, 16 Oct 2020 07:19:15: Done! pass1 - making usageList (60 chroms): 1 millis pass2 - checking and writing primary data (1886 records, 4 fields): 6 millis CompletedMACS2peakCalling INFO @ Fri, 16 Oct 2020 07:19:47: #4 Write output xls file... /home/okishinya/chipatlas/results/rn6/ERX2745138/ERX2745138.20_peaks.xls INFO @ Fri, 16 Oct 2020 07:19:47: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/rn6/ERX2745138/ERX2745138.20_peaks.narrowPeak INFO @ Fri, 16 Oct 2020 07:19:47: #4 Write summits bed file... /home/okishinya/chipatlas/results/rn6/ERX2745138/ERX2745138.20_summits.bed INFO @ Fri, 16 Oct 2020 07:19:47: Done! pass1 - making usageList (42 chroms): 1 millis pass2 - checking and writing primary data (814 records, 4 fields): 3 millis CompletedMACS2peakCalling