Job ID = 10223660 SRX = ERX2745135 Genome = rn6 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-10-15T20:15:55 prefetch.2.10.7: 1) Downloading 'ERR2731910'... 2020-10-15T20:15:55 prefetch.2.10.7: Downloading via HTTPS... 2020-10-15T20:18:14 prefetch.2.10.7: HTTPS download succeed 2020-10-15T20:18:14 prefetch.2.10.7: 1) 'ERR2731910' was downloaded successfully Read 97724732 spots for ERR2731910/ERR2731910.sra Written 97724732 spots for ERR2731910/ERR2731910.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:01 Time loading forward index: 00:00:04 Time loading mirror index: 00:00:01 Multiseed full-index search: 01:09:09 97724732 reads; of these: 97724732 (100.00%) were unpaired; of these: 2344590 (2.40%) aligned 0 times 66762899 (68.32%) aligned exactly 1 time 28617243 (29.28%) aligned >1 times 97.60% overall alignment rate Time searching: 01:09:16 Overall time: 01:09:16 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 953 sequences. [bam_sort_core] merging from 40 files... [bam_rmdupse_core] 21717172 / 95380142 = 0.2277 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 06:48:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/rn6/ERX2745135/ERX2745135.bam -f BAM -g 2.15e9 -n /home/okishinya/chipatlas/results/rn6/ERX2745135/ERX2745135.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/rn6/ERX2745135/ERX2745135.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/rn6/ERX2745135/ERX2745135.bam'] # control file = None # effective genome size = 2.15e+09 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 06:48:25: #1 read tag files... INFO @ Fri, 16 Oct 2020 06:48:25: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 06:48:31: 1000000 INFO @ Fri, 16 Oct 2020 06:48:37: 2000000 INFO @ Fri, 16 Oct 2020 06:48:44: 3000000 INFO @ Fri, 16 Oct 2020 06:48:50: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 06:48:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/rn6/ERX2745135/ERX2745135.bam -f BAM -g 2.15e9 -n /home/okishinya/chipatlas/results/rn6/ERX2745135/ERX2745135.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/rn6/ERX2745135/ERX2745135.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/rn6/ERX2745135/ERX2745135.bam'] # control file = None # effective genome size = 2.15e+09 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 06:48:54: #1 read tag files... INFO @ Fri, 16 Oct 2020 06:48:54: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 06:48:57: 5000000 INFO @ Fri, 16 Oct 2020 06:49:01: 1000000 INFO @ Fri, 16 Oct 2020 06:49:03: 6000000 INFO @ Fri, 16 Oct 2020 06:49:08: 2000000 INFO @ Fri, 16 Oct 2020 06:49:10: 7000000 INFO @ Fri, 16 Oct 2020 06:49:14: 3000000 INFO @ Fri, 16 Oct 2020 06:49:16: 8000000 INFO @ Fri, 16 Oct 2020 06:49:21: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 16 Oct 2020 06:49:23: 9000000 INFO @ Fri, 16 Oct 2020 06:49:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/rn6/ERX2745135/ERX2745135.bam -f BAM -g 2.15e9 -n /home/okishinya/chipatlas/results/rn6/ERX2745135/ERX2745135.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/rn6/ERX2745135/ERX2745135.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/rn6/ERX2745135/ERX2745135.bam'] # control file = None # effective genome size = 2.15e+09 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Oct 2020 06:49:25: #1 read tag files... INFO @ Fri, 16 Oct 2020 06:49:25: #1 read treatment tags... INFO @ Fri, 16 Oct 2020 06:49:28: 5000000 INFO @ Fri, 16 Oct 2020 06:49:30: 10000000 INFO @ Fri, 16 Oct 2020 06:49:32: 1000000 INFO @ Fri, 16 Oct 2020 06:49:35: 6000000 INFO @ Fri, 16 Oct 2020 06:49:37: 11000000 INFO @ Fri, 16 Oct 2020 06:49:39: 2000000 INFO @ Fri, 16 Oct 2020 06:49:42: 7000000 INFO @ Fri, 16 Oct 2020 06:49:44: 12000000 INFO @ Fri, 16 Oct 2020 06:49:46: 3000000 INFO @ Fri, 16 Oct 2020 06:49:49: 8000000 INFO @ Fri, 16 Oct 2020 06:49:51: 13000000 INFO @ Fri, 16 Oct 2020 06:49:54: 4000000 INFO @ Fri, 16 Oct 2020 06:49:57: 9000000 INFO @ Fri, 16 Oct 2020 06:49:58: 14000000 INFO @ Fri, 16 Oct 2020 06:50:01: 5000000 INFO @ Fri, 16 Oct 2020 06:50:04: 10000000 INFO @ Fri, 16 Oct 2020 06:50:05: 15000000 INFO @ Fri, 16 Oct 2020 06:50:08: 6000000 INFO @ Fri, 16 Oct 2020 06:50:11: 11000000 INFO @ Fri, 16 Oct 2020 06:50:12: 16000000 INFO @ Fri, 16 Oct 2020 06:50:15: 7000000 INFO @ Fri, 16 Oct 2020 06:50:18: 12000000 INFO @ Fri, 16 Oct 2020 06:50:19: 17000000 INFO @ Fri, 16 Oct 2020 06:50:22: 8000000 INFO @ Fri, 16 Oct 2020 06:50:25: 13000000 INFO @ Fri, 16 Oct 2020 06:50:26: 18000000 INFO @ Fri, 16 Oct 2020 06:50:29: 9000000 INFO @ Fri, 16 Oct 2020 06:50:33: 14000000 INFO @ Fri, 16 Oct 2020 06:50:33: 19000000 INFO @ Fri, 16 Oct 2020 06:50:37: 10000000 INFO @ Fri, 16 Oct 2020 06:50:40: 15000000 INFO @ Fri, 16 Oct 2020 06:50:40: 20000000 INFO @ Fri, 16 Oct 2020 06:50:44: 11000000 INFO @ Fri, 16 Oct 2020 06:50:47: 16000000 INFO @ Fri, 16 Oct 2020 06:50:47: 21000000 INFO @ Fri, 16 Oct 2020 06:50:51: 12000000 INFO @ Fri, 16 Oct 2020 06:50:54: 17000000 INFO @ Fri, 16 Oct 2020 06:50:54: 22000000 INFO @ Fri, 16 Oct 2020 06:50:58: 13000000 INFO @ Fri, 16 Oct 2020 06:51:01: 18000000 INFO @ Fri, 16 Oct 2020 06:51:01: 23000000 INFO @ Fri, 16 Oct 2020 06:51:05: 14000000 INFO @ Fri, 16 Oct 2020 06:51:07: 19000000 INFO @ Fri, 16 Oct 2020 06:51:08: 24000000 INFO @ Fri, 16 Oct 2020 06:51:12: 15000000 INFO @ Fri, 16 Oct 2020 06:51:15: 20000000 INFO @ Fri, 16 Oct 2020 06:51:15: 25000000 INFO @ Fri, 16 Oct 2020 06:51:19: 16000000 INFO @ Fri, 16 Oct 2020 06:51:22: 21000000 INFO @ Fri, 16 Oct 2020 06:51:22: 26000000 INFO @ Fri, 16 Oct 2020 06:51:26: 17000000 INFO @ Fri, 16 Oct 2020 06:51:29: 22000000 INFO @ Fri, 16 Oct 2020 06:51:30: 27000000 INFO @ Fri, 16 Oct 2020 06:51:34: 18000000 INFO @ Fri, 16 Oct 2020 06:51:36: 23000000 INFO @ Fri, 16 Oct 2020 06:51:37: 28000000 INFO @ Fri, 16 Oct 2020 06:51:41: 19000000 INFO @ Fri, 16 Oct 2020 06:51:43: 24000000 INFO @ Fri, 16 Oct 2020 06:51:44: 29000000 INFO @ Fri, 16 Oct 2020 06:51:48: 20000000 INFO @ Fri, 16 Oct 2020 06:51:51: 25000000 INFO @ Fri, 16 Oct 2020 06:51:52: 30000000 INFO @ Fri, 16 Oct 2020 06:51:55: 21000000 INFO @ Fri, 16 Oct 2020 06:51:58: 26000000 INFO @ Fri, 16 Oct 2020 06:51:59: 31000000 INFO @ Fri, 16 Oct 2020 06:52:03: 22000000 INFO @ Fri, 16 Oct 2020 06:52:05: 27000000 INFO @ Fri, 16 Oct 2020 06:52:06: 32000000 INFO @ Fri, 16 Oct 2020 06:52:10: 23000000 INFO @ Fri, 16 Oct 2020 06:52:12: 28000000 INFO @ Fri, 16 Oct 2020 06:52:13: 33000000 INFO @ Fri, 16 Oct 2020 06:52:17: 24000000 INFO @ Fri, 16 Oct 2020 06:52:20: 29000000 INFO @ Fri, 16 Oct 2020 06:52:20: 34000000 INFO @ Fri, 16 Oct 2020 06:52:24: 25000000 INFO @ Fri, 16 Oct 2020 06:52:27: 30000000 INFO @ Fri, 16 Oct 2020 06:52:28: 35000000 INFO @ Fri, 16 Oct 2020 06:52:32: 26000000 INFO @ Fri, 16 Oct 2020 06:52:34: 31000000 INFO @ Fri, 16 Oct 2020 06:52:35: 36000000 INFO @ Fri, 16 Oct 2020 06:52:39: 27000000 INFO @ Fri, 16 Oct 2020 06:52:41: 32000000 INFO @ Fri, 16 Oct 2020 06:52:42: 37000000 INFO @ Fri, 16 Oct 2020 06:52:46: 28000000 INFO @ Fri, 16 Oct 2020 06:52:48: 33000000 INFO @ Fri, 16 Oct 2020 06:52:49: 38000000 INFO @ Fri, 16 Oct 2020 06:52:53: 29000000 INFO @ Fri, 16 Oct 2020 06:52:55: 34000000 INFO @ Fri, 16 Oct 2020 06:52:56: 39000000 INFO @ Fri, 16 Oct 2020 06:53:01: 30000000 INFO @ Fri, 16 Oct 2020 06:53:02: 35000000 INFO @ Fri, 16 Oct 2020 06:53:04: 40000000 INFO @ Fri, 16 Oct 2020 06:53:08: 31000000 INFO @ Fri, 16 Oct 2020 06:53:09: 36000000 INFO @ Fri, 16 Oct 2020 06:53:11: 41000000 INFO @ Fri, 16 Oct 2020 06:53:15: 32000000 INFO @ Fri, 16 Oct 2020 06:53:16: 37000000 INFO @ Fri, 16 Oct 2020 06:53:18: 42000000 INFO @ Fri, 16 Oct 2020 06:53:22: 33000000 INFO @ Fri, 16 Oct 2020 06:53:23: 38000000 INFO @ Fri, 16 Oct 2020 06:53:26: 43000000 INFO @ Fri, 16 Oct 2020 06:53:29: 34000000 INFO @ Fri, 16 Oct 2020 06:53:31: 39000000 INFO @ Fri, 16 Oct 2020 06:53:33: 44000000 INFO @ Fri, 16 Oct 2020 06:53:36: 35000000 INFO @ Fri, 16 Oct 2020 06:53:38: 40000000 INFO @ Fri, 16 Oct 2020 06:53:40: 45000000 INFO @ Fri, 16 Oct 2020 06:53:43: 36000000 INFO @ Fri, 16 Oct 2020 06:53:45: 41000000 INFO @ Fri, 16 Oct 2020 06:53:47: 46000000 INFO @ Fri, 16 Oct 2020 06:53:50: 37000000 INFO @ Fri, 16 Oct 2020 06:53:52: 42000000 INFO @ Fri, 16 Oct 2020 06:53:54: 47000000 INFO @ Fri, 16 Oct 2020 06:53:57: 38000000 INFO @ Fri, 16 Oct 2020 06:53:59: 43000000 INFO @ Fri, 16 Oct 2020 06:54:01: 48000000 INFO @ Fri, 16 Oct 2020 06:54:05: 39000000 INFO @ Fri, 16 Oct 2020 06:54:06: 44000000 INFO @ Fri, 16 Oct 2020 06:54:08: 49000000 INFO @ Fri, 16 Oct 2020 06:54:12: 40000000 INFO @ Fri, 16 Oct 2020 06:54:13: 45000000 INFO @ Fri, 16 Oct 2020 06:54:15: 50000000 INFO @ Fri, 16 Oct 2020 06:54:19: 41000000 INFO @ Fri, 16 Oct 2020 06:54:20: 46000000 INFO @ Fri, 16 Oct 2020 06:54:22: 51000000 INFO @ Fri, 16 Oct 2020 06:54:26: 42000000 INFO @ Fri, 16 Oct 2020 06:54:27: 47000000 INFO @ Fri, 16 Oct 2020 06:54:29: 52000000 INFO @ Fri, 16 Oct 2020 06:54:33: 43000000 INFO @ Fri, 16 Oct 2020 06:54:34: 48000000 INFO @ Fri, 16 Oct 2020 06:54:37: 53000000 INFO @ Fri, 16 Oct 2020 06:54:40: 44000000 INFO @ Fri, 16 Oct 2020 06:54:41: 49000000 INFO @ Fri, 16 Oct 2020 06:54:44: 54000000 INFO @ Fri, 16 Oct 2020 06:54:47: 45000000 INFO @ Fri, 16 Oct 2020 06:54:48: 50000000 INFO @ Fri, 16 Oct 2020 06:54:51: 55000000 INFO @ Fri, 16 Oct 2020 06:54:54: 46000000 INFO @ Fri, 16 Oct 2020 06:54:55: 51000000 INFO @ Fri, 16 Oct 2020 06:54:58: 56000000 INFO @ Fri, 16 Oct 2020 06:55:01: 47000000 INFO @ Fri, 16 Oct 2020 06:55:02: 52000000 INFO @ Fri, 16 Oct 2020 06:55:05: 57000000 INFO @ Fri, 16 Oct 2020 06:55:08: 48000000 INFO @ Fri, 16 Oct 2020 06:55:09: 53000000 INFO @ Fri, 16 Oct 2020 06:55:12: 58000000 INFO @ Fri, 16 Oct 2020 06:55:15: 49000000 INFO @ Fri, 16 Oct 2020 06:55:16: 54000000 INFO @ Fri, 16 Oct 2020 06:55:20: 59000000 INFO @ Fri, 16 Oct 2020 06:55:22: 50000000 INFO @ Fri, 16 Oct 2020 06:55:23: 55000000 INFO @ Fri, 16 Oct 2020 06:55:27: 60000000 INFO @ Fri, 16 Oct 2020 06:55:30: 51000000 INFO @ Fri, 16 Oct 2020 06:55:30: 56000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 16 Oct 2020 06:55:34: 61000000 INFO @ Fri, 16 Oct 2020 06:55:37: 52000000 INFO @ Fri, 16 Oct 2020 06:55:37: 57000000 INFO @ Fri, 16 Oct 2020 06:55:41: 62000000 INFO @ Fri, 16 Oct 2020 06:55:44: 53000000 INFO @ Fri, 16 Oct 2020 06:55:44: 58000000 INFO @ Fri, 16 Oct 2020 06:55:48: 63000000 INFO @ Fri, 16 Oct 2020 06:55:51: 54000000 INFO @ Fri, 16 Oct 2020 06:55:51: 59000000 INFO @ Fri, 16 Oct 2020 06:55:55: 64000000 INFO @ Fri, 16 Oct 2020 06:55:58: 55000000 INFO @ Fri, 16 Oct 2020 06:55:58: 60000000 INFO @ Fri, 16 Oct 2020 06:56:03: 65000000 INFO @ Fri, 16 Oct 2020 06:56:05: 56000000 INFO @ Fri, 16 Oct 2020 06:56:05: 61000000 INFO @ Fri, 16 Oct 2020 06:56:10: 66000000 INFO @ Fri, 16 Oct 2020 06:56:12: 57000000 INFO @ Fri, 16 Oct 2020 06:56:13: 62000000 INFO @ Fri, 16 Oct 2020 06:56:17: 67000000 INFO @ Fri, 16 Oct 2020 06:56:20: 58000000 INFO @ Fri, 16 Oct 2020 06:56:20: 63000000 INFO @ Fri, 16 Oct 2020 06:56:24: 68000000 INFO @ Fri, 16 Oct 2020 06:56:27: 64000000 INFO @ Fri, 16 Oct 2020 06:56:27: 59000000 INFO @ Fri, 16 Oct 2020 06:56:31: 69000000 INFO @ Fri, 16 Oct 2020 06:56:34: 65000000 INFO @ Fri, 16 Oct 2020 06:56:34: 60000000 INFO @ Fri, 16 Oct 2020 06:56:38: 70000000 INFO @ Fri, 16 Oct 2020 06:56:41: 66000000 INFO @ Fri, 16 Oct 2020 06:56:41: 61000000 INFO @ Fri, 16 Oct 2020 06:56:45: 71000000 INFO @ Fri, 16 Oct 2020 06:56:48: 67000000 INFO @ Fri, 16 Oct 2020 06:56:48: 62000000 INFO @ Fri, 16 Oct 2020 06:56:52: 72000000 INFO @ Fri, 16 Oct 2020 06:56:55: 68000000 INFO @ Fri, 16 Oct 2020 06:56:55: 63000000 INFO @ Fri, 16 Oct 2020 06:57:00: 73000000 INFO @ Fri, 16 Oct 2020 06:57:02: 69000000 INFO @ Fri, 16 Oct 2020 06:57:02: 64000000 INFO @ Fri, 16 Oct 2020 06:57:05: #1 tag size is determined as 50 bps INFO @ Fri, 16 Oct 2020 06:57:05: #1 tag size = 50 INFO @ Fri, 16 Oct 2020 06:57:05: #1 total tags in treatment: 73662970 INFO @ Fri, 16 Oct 2020 06:57:05: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 06:57:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 06:57:06: #1 tags after filtering in treatment: 73662952 INFO @ Fri, 16 Oct 2020 06:57:06: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 16 Oct 2020 06:57:06: #1 finished! INFO @ Fri, 16 Oct 2020 06:57:06: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 06:57:06: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 06:57:09: 70000000 INFO @ Fri, 16 Oct 2020 06:57:09: 65000000 INFO @ Fri, 16 Oct 2020 06:57:12: #2 number of paired peaks: 6572 INFO @ Fri, 16 Oct 2020 06:57:12: start model_add_line... INFO @ Fri, 16 Oct 2020 06:57:13: start X-correlation... INFO @ Fri, 16 Oct 2020 06:57:13: end of X-cor INFO @ Fri, 16 Oct 2020 06:57:13: #2 finished! INFO @ Fri, 16 Oct 2020 06:57:13: #2 predicted fragment length is 50 bps INFO @ Fri, 16 Oct 2020 06:57:13: #2 alternative fragment length(s) may be 50 bps INFO @ Fri, 16 Oct 2020 06:57:13: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/rn6/ERX2745135/ERX2745135.05_model.r WARNING @ Fri, 16 Oct 2020 06:57:13: #2 Since the d (50) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 16 Oct 2020 06:57:13: #2 You may need to consider one of the other alternative d(s): 50 WARNING @ Fri, 16 Oct 2020 06:57:13: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 16 Oct 2020 06:57:13: #3 Call peaks... INFO @ Fri, 16 Oct 2020 06:57:13: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 16 Oct 2020 06:57:16: 71000000 INFO @ Fri, 16 Oct 2020 06:57:16: 66000000 INFO @ Fri, 16 Oct 2020 06:57:23: 72000000 INFO @ Fri, 16 Oct 2020 06:57:23: 67000000 INFO @ Fri, 16 Oct 2020 06:57:30: 73000000 INFO @ Fri, 16 Oct 2020 06:57:30: 68000000 INFO @ Fri, 16 Oct 2020 06:57:35: #1 tag size is determined as 50 bps INFO @ Fri, 16 Oct 2020 06:57:35: #1 tag size = 50 INFO @ Fri, 16 Oct 2020 06:57:35: #1 total tags in treatment: 73662970 INFO @ Fri, 16 Oct 2020 06:57:35: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 06:57:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 06:57:36: #1 tags after filtering in treatment: 73662952 INFO @ Fri, 16 Oct 2020 06:57:36: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 16 Oct 2020 06:57:36: #1 finished! INFO @ Fri, 16 Oct 2020 06:57:36: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 06:57:36: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 06:57:37: 69000000 INFO @ Fri, 16 Oct 2020 06:57:42: #2 number of paired peaks: 6572 INFO @ Fri, 16 Oct 2020 06:57:42: start model_add_line... INFO @ Fri, 16 Oct 2020 06:57:43: start X-correlation... INFO @ Fri, 16 Oct 2020 06:57:43: end of X-cor INFO @ Fri, 16 Oct 2020 06:57:43: #2 finished! INFO @ Fri, 16 Oct 2020 06:57:43: #2 predicted fragment length is 50 bps INFO @ Fri, 16 Oct 2020 06:57:43: #2 alternative fragment length(s) may be 50 bps INFO @ Fri, 16 Oct 2020 06:57:43: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/rn6/ERX2745135/ERX2745135.10_model.r WARNING @ Fri, 16 Oct 2020 06:57:43: #2 Since the d (50) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 16 Oct 2020 06:57:43: #2 You may need to consider one of the other alternative d(s): 50 WARNING @ Fri, 16 Oct 2020 06:57:43: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 16 Oct 2020 06:57:43: #3 Call peaks... INFO @ Fri, 16 Oct 2020 06:57:43: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 16 Oct 2020 06:57:44: 70000000 INFO @ Fri, 16 Oct 2020 06:57:50: 71000000 INFO @ Fri, 16 Oct 2020 06:57:57: 72000000 INFO @ Fri, 16 Oct 2020 06:58:03: 73000000 INFO @ Fri, 16 Oct 2020 06:58:08: #1 tag size is determined as 50 bps INFO @ Fri, 16 Oct 2020 06:58:08: #1 tag size = 50 INFO @ Fri, 16 Oct 2020 06:58:08: #1 total tags in treatment: 73662970 INFO @ Fri, 16 Oct 2020 06:58:08: #1 user defined the maximum tags... INFO @ Fri, 16 Oct 2020 06:58:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Oct 2020 06:58:09: #1 tags after filtering in treatment: 73662952 INFO @ Fri, 16 Oct 2020 06:58:09: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 16 Oct 2020 06:58:09: #1 finished! INFO @ Fri, 16 Oct 2020 06:58:09: #2 Build Peak Model... INFO @ Fri, 16 Oct 2020 06:58:09: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Oct 2020 06:58:15: #2 number of paired peaks: 6572 INFO @ Fri, 16 Oct 2020 06:58:15: start model_add_line... INFO @ Fri, 16 Oct 2020 06:58:16: start X-correlation... INFO @ Fri, 16 Oct 2020 06:58:16: end of X-cor INFO @ Fri, 16 Oct 2020 06:58:16: #2 finished! INFO @ Fri, 16 Oct 2020 06:58:16: #2 predicted fragment length is 50 bps INFO @ Fri, 16 Oct 2020 06:58:16: #2 alternative fragment length(s) may be 50 bps INFO @ Fri, 16 Oct 2020 06:58:16: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/rn6/ERX2745135/ERX2745135.20_model.r WARNING @ Fri, 16 Oct 2020 06:58:16: #2 Since the d (50) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 16 Oct 2020 06:58:16: #2 You may need to consider one of the other alternative d(s): 50 WARNING @ Fri, 16 Oct 2020 06:58:16: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 16 Oct 2020 06:58:16: #3 Call peaks... INFO @ Fri, 16 Oct 2020 06:58:16: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Fri, 16 Oct 2020 07:00:02: #3 Call peaks for each chromosome... INFO @ Fri, 16 Oct 2020 07:00:35: #3 Call peaks for each chromosome... INFO @ Fri, 16 Oct 2020 07:01:02: #3 Call peaks for each chromosome... INFO @ Fri, 16 Oct 2020 07:01:33: #4 Write output xls file... /home/okishinya/chipatlas/results/rn6/ERX2745135/ERX2745135.05_peaks.xls INFO @ Fri, 16 Oct 2020 07:01:33: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/rn6/ERX2745135/ERX2745135.05_peaks.narrowPeak INFO @ Fri, 16 Oct 2020 07:01:33: #4 Write summits bed file... /home/okishinya/chipatlas/results/rn6/ERX2745135/ERX2745135.05_summits.bed INFO @ Fri, 16 Oct 2020 07:01:33: Done! pass1 - making usageList (76 chroms): 1 millis pass2 - checking and writing primary data (3729 records, 4 fields): 8 millis CompletedMACS2peakCalling INFO @ Fri, 16 Oct 2020 07:02:04: #4 Write output xls file... /home/okishinya/chipatlas/results/rn6/ERX2745135/ERX2745135.10_peaks.xls INFO @ Fri, 16 Oct 2020 07:02:04: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/rn6/ERX2745135/ERX2745135.10_peaks.narrowPeak INFO @ Fri, 16 Oct 2020 07:02:04: #4 Write summits bed file... /home/okishinya/chipatlas/results/rn6/ERX2745135/ERX2745135.10_summits.bed INFO @ Fri, 16 Oct 2020 07:02:04: Done! pass1 - making usageList (60 chroms): 1 millis pass2 - checking and writing primary data (1942 records, 4 fields): 5 millis CompletedMACS2peakCalling INFO @ Fri, 16 Oct 2020 07:02:38: #4 Write output xls file... /home/okishinya/chipatlas/results/rn6/ERX2745135/ERX2745135.20_peaks.xls INFO @ Fri, 16 Oct 2020 07:02:38: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/rn6/ERX2745135/ERX2745135.20_peaks.narrowPeak INFO @ Fri, 16 Oct 2020 07:02:38: #4 Write summits bed file... /home/okishinya/chipatlas/results/rn6/ERX2745135/ERX2745135.20_summits.bed INFO @ Fri, 16 Oct 2020 07:02:38: Done! pass1 - making usageList (45 chroms): 1 millis pass2 - checking and writing primary data (826 records, 4 fields): 4 millis CompletedMACS2peakCalling