Job ID = 6368987 SRX = SRX7971812 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-16T00:37:19 prefetch.2.10.7: 1) Downloading 'SRR11392698'... 2020-06-16T00:37:19 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:50:07 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:50:07 prefetch.2.10.7: 1) 'SRR11392698' was downloaded successfully Read 71206836 spots for SRR11392698/SRR11392698.sra Written 71206836 spots for SRR11392698/SRR11392698.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:24:11 71206836 reads; of these: 71206836 (100.00%) were unpaired; of these: 645879 (0.91%) aligned 0 times 59276626 (83.25%) aligned exactly 1 time 11284331 (15.85%) aligned >1 times 99.09% overall alignment rate Time searching: 00:24:11 Overall time: 00:24:11 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 32 files... [bam_rmdupse_core] 16943596 / 70560957 = 0.2401 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:37:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7971812/SRX7971812.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7971812/SRX7971812.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7971812/SRX7971812.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7971812/SRX7971812.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:37:13: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:37:13: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:37:19: 1000000 INFO @ Tue, 16 Jun 2020 10:37:25: 2000000 INFO @ Tue, 16 Jun 2020 10:37:31: 3000000 INFO @ Tue, 16 Jun 2020 10:37:36: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:37:43: 5000000 INFO @ Tue, 16 Jun 2020 10:37:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7971812/SRX7971812.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7971812/SRX7971812.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7971812/SRX7971812.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7971812/SRX7971812.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:37:43: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:37:43: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:37:49: 1000000 INFO @ Tue, 16 Jun 2020 10:37:50: 6000000 INFO @ Tue, 16 Jun 2020 10:37:55: 2000000 INFO @ Tue, 16 Jun 2020 10:37:56: 7000000 INFO @ Tue, 16 Jun 2020 10:38:01: 3000000 INFO @ Tue, 16 Jun 2020 10:38:02: 8000000 INFO @ Tue, 16 Jun 2020 10:38:07: 4000000 INFO @ Tue, 16 Jun 2020 10:38:08: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:38:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7971812/SRX7971812.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7971812/SRX7971812.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7971812/SRX7971812.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7971812/SRX7971812.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:38:13: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:38:13: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:38:13: 5000000 INFO @ Tue, 16 Jun 2020 10:38:14: 10000000 INFO @ Tue, 16 Jun 2020 10:38:19: 1000000 INFO @ Tue, 16 Jun 2020 10:38:20: 6000000 INFO @ Tue, 16 Jun 2020 10:38:20: 11000000 INFO @ Tue, 16 Jun 2020 10:38:25: 2000000 INFO @ Tue, 16 Jun 2020 10:38:26: 12000000 INFO @ Tue, 16 Jun 2020 10:38:26: 7000000 INFO @ Tue, 16 Jun 2020 10:38:31: 3000000 INFO @ Tue, 16 Jun 2020 10:38:32: 13000000 INFO @ Tue, 16 Jun 2020 10:38:32: 8000000 INFO @ Tue, 16 Jun 2020 10:38:37: 4000000 INFO @ Tue, 16 Jun 2020 10:38:38: 14000000 INFO @ Tue, 16 Jun 2020 10:38:39: 9000000 INFO @ Tue, 16 Jun 2020 10:38:43: 5000000 INFO @ Tue, 16 Jun 2020 10:38:45: 15000000 INFO @ Tue, 16 Jun 2020 10:38:45: 10000000 INFO @ Tue, 16 Jun 2020 10:38:50: 6000000 INFO @ Tue, 16 Jun 2020 10:38:51: 11000000 INFO @ Tue, 16 Jun 2020 10:38:51: 16000000 INFO @ Tue, 16 Jun 2020 10:38:56: 7000000 INFO @ Tue, 16 Jun 2020 10:38:57: 12000000 INFO @ Tue, 16 Jun 2020 10:38:57: 17000000 INFO @ Tue, 16 Jun 2020 10:39:02: 8000000 INFO @ Tue, 16 Jun 2020 10:39:03: 13000000 INFO @ Tue, 16 Jun 2020 10:39:03: 18000000 INFO @ Tue, 16 Jun 2020 10:39:09: 9000000 INFO @ Tue, 16 Jun 2020 10:39:09: 14000000 INFO @ Tue, 16 Jun 2020 10:39:09: 19000000 INFO @ Tue, 16 Jun 2020 10:39:15: 10000000 INFO @ Tue, 16 Jun 2020 10:39:15: 15000000 INFO @ Tue, 16 Jun 2020 10:39:15: 20000000 INFO @ Tue, 16 Jun 2020 10:39:21: 11000000 INFO @ Tue, 16 Jun 2020 10:39:21: 16000000 INFO @ Tue, 16 Jun 2020 10:39:21: 21000000 INFO @ Tue, 16 Jun 2020 10:39:27: 12000000 INFO @ Tue, 16 Jun 2020 10:39:27: 17000000 INFO @ Tue, 16 Jun 2020 10:39:27: 22000000 INFO @ Tue, 16 Jun 2020 10:39:33: 13000000 INFO @ Tue, 16 Jun 2020 10:39:33: 18000000 INFO @ Tue, 16 Jun 2020 10:39:33: 23000000 INFO @ Tue, 16 Jun 2020 10:39:39: 14000000 INFO @ Tue, 16 Jun 2020 10:39:39: 19000000 INFO @ Tue, 16 Jun 2020 10:39:39: 24000000 INFO @ Tue, 16 Jun 2020 10:39:45: 15000000 INFO @ Tue, 16 Jun 2020 10:39:45: 20000000 INFO @ Tue, 16 Jun 2020 10:39:46: 25000000 INFO @ Tue, 16 Jun 2020 10:39:51: 16000000 INFO @ Tue, 16 Jun 2020 10:39:51: 21000000 INFO @ Tue, 16 Jun 2020 10:39:52: 26000000 INFO @ Tue, 16 Jun 2020 10:39:57: 17000000 INFO @ Tue, 16 Jun 2020 10:39:58: 22000000 INFO @ Tue, 16 Jun 2020 10:39:58: 27000000 INFO @ Tue, 16 Jun 2020 10:40:03: 18000000 INFO @ Tue, 16 Jun 2020 10:40:04: 23000000 INFO @ Tue, 16 Jun 2020 10:40:04: 28000000 INFO @ Tue, 16 Jun 2020 10:40:09: 19000000 INFO @ Tue, 16 Jun 2020 10:40:10: 24000000 INFO @ Tue, 16 Jun 2020 10:40:10: 29000000 INFO @ Tue, 16 Jun 2020 10:40:15: 20000000 INFO @ Tue, 16 Jun 2020 10:40:16: 25000000 INFO @ Tue, 16 Jun 2020 10:40:16: 30000000 INFO @ Tue, 16 Jun 2020 10:40:22: 21000000 INFO @ Tue, 16 Jun 2020 10:40:22: 26000000 INFO @ Tue, 16 Jun 2020 10:40:22: 31000000 INFO @ Tue, 16 Jun 2020 10:40:28: 22000000 INFO @ Tue, 16 Jun 2020 10:40:28: 27000000 INFO @ Tue, 16 Jun 2020 10:40:29: 32000000 INFO @ Tue, 16 Jun 2020 10:40:34: 23000000 INFO @ Tue, 16 Jun 2020 10:40:34: 28000000 INFO @ Tue, 16 Jun 2020 10:40:35: 33000000 INFO @ Tue, 16 Jun 2020 10:40:40: 24000000 INFO @ Tue, 16 Jun 2020 10:40:41: 29000000 INFO @ Tue, 16 Jun 2020 10:40:41: 34000000 INFO @ Tue, 16 Jun 2020 10:40:46: 25000000 INFO @ Tue, 16 Jun 2020 10:40:47: 30000000 INFO @ Tue, 16 Jun 2020 10:40:47: 35000000 INFO @ Tue, 16 Jun 2020 10:40:52: 26000000 INFO @ Tue, 16 Jun 2020 10:40:53: 31000000 INFO @ Tue, 16 Jun 2020 10:40:53: 36000000 INFO @ Tue, 16 Jun 2020 10:40:58: 27000000 INFO @ Tue, 16 Jun 2020 10:40:59: 32000000 INFO @ Tue, 16 Jun 2020 10:40:59: 37000000 INFO @ Tue, 16 Jun 2020 10:41:04: 28000000 INFO @ Tue, 16 Jun 2020 10:41:05: 33000000 INFO @ Tue, 16 Jun 2020 10:41:05: 38000000 INFO @ Tue, 16 Jun 2020 10:41:10: 29000000 INFO @ Tue, 16 Jun 2020 10:41:11: 34000000 INFO @ Tue, 16 Jun 2020 10:41:11: 39000000 INFO @ Tue, 16 Jun 2020 10:41:16: 30000000 INFO @ Tue, 16 Jun 2020 10:41:17: 35000000 INFO @ Tue, 16 Jun 2020 10:41:17: 40000000 INFO @ Tue, 16 Jun 2020 10:41:22: 31000000 INFO @ Tue, 16 Jun 2020 10:41:23: 36000000 INFO @ Tue, 16 Jun 2020 10:41:23: 41000000 INFO @ Tue, 16 Jun 2020 10:41:28: 32000000 INFO @ Tue, 16 Jun 2020 10:41:29: 37000000 INFO @ Tue, 16 Jun 2020 10:41:30: 42000000 INFO @ Tue, 16 Jun 2020 10:41:34: 33000000 INFO @ Tue, 16 Jun 2020 10:41:35: 38000000 INFO @ Tue, 16 Jun 2020 10:41:36: 43000000 INFO @ Tue, 16 Jun 2020 10:41:40: 34000000 INFO @ Tue, 16 Jun 2020 10:41:41: 39000000 INFO @ Tue, 16 Jun 2020 10:41:42: 44000000 INFO @ Tue, 16 Jun 2020 10:41:46: 35000000 INFO @ Tue, 16 Jun 2020 10:41:47: 40000000 INFO @ Tue, 16 Jun 2020 10:41:48: 45000000 INFO @ Tue, 16 Jun 2020 10:41:52: 36000000 INFO @ Tue, 16 Jun 2020 10:41:54: 41000000 INFO @ Tue, 16 Jun 2020 10:41:54: 46000000 INFO @ Tue, 16 Jun 2020 10:41:59: 37000000 INFO @ Tue, 16 Jun 2020 10:42:00: 42000000 INFO @ Tue, 16 Jun 2020 10:42:00: 47000000 INFO @ Tue, 16 Jun 2020 10:42:05: 38000000 INFO @ Tue, 16 Jun 2020 10:42:06: 43000000 INFO @ Tue, 16 Jun 2020 10:42:06: 48000000 INFO @ Tue, 16 Jun 2020 10:42:11: 39000000 INFO @ Tue, 16 Jun 2020 10:42:12: 44000000 INFO @ Tue, 16 Jun 2020 10:42:13: 49000000 INFO @ Tue, 16 Jun 2020 10:42:18: 40000000 INFO @ Tue, 16 Jun 2020 10:42:19: 45000000 INFO @ Tue, 16 Jun 2020 10:42:19: 50000000 INFO @ Tue, 16 Jun 2020 10:42:24: 41000000 INFO @ Tue, 16 Jun 2020 10:42:25: 46000000 INFO @ Tue, 16 Jun 2020 10:42:26: 51000000 INFO @ Tue, 16 Jun 2020 10:42:31: 42000000 INFO @ Tue, 16 Jun 2020 10:42:31: 47000000 INFO @ Tue, 16 Jun 2020 10:42:32: 52000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 10:42:37: 43000000 INFO @ Tue, 16 Jun 2020 10:42:37: 48000000 INFO @ Tue, 16 Jun 2020 10:42:39: 53000000 INFO @ Tue, 16 Jun 2020 10:42:43: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 10:42:43: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 10:42:43: #1 total tags in treatment: 53617361 INFO @ Tue, 16 Jun 2020 10:42:43: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:42:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:42:44: 49000000 INFO @ Tue, 16 Jun 2020 10:42:44: #1 tags after filtering in treatment: 53617361 INFO @ Tue, 16 Jun 2020 10:42:44: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:42:44: #1 finished! INFO @ Tue, 16 Jun 2020 10:42:44: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:42:44: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:42:44: 44000000 INFO @ Tue, 16 Jun 2020 10:42:47: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 10:42:47: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:42:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7971812/SRX7971812.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971812/SRX7971812.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971812/SRX7971812.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971812/SRX7971812.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:42:50: 50000000 INFO @ Tue, 16 Jun 2020 10:42:51: 45000000 INFO @ Tue, 16 Jun 2020 10:42:56: 51000000 INFO @ Tue, 16 Jun 2020 10:42:57: 46000000 INFO @ Tue, 16 Jun 2020 10:43:02: 52000000 INFO @ Tue, 16 Jun 2020 10:43:03: 47000000 INFO @ Tue, 16 Jun 2020 10:43:09: 53000000 INFO @ Tue, 16 Jun 2020 10:43:10: 48000000 INFO @ Tue, 16 Jun 2020 10:43:13: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 10:43:13: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 10:43:13: #1 total tags in treatment: 53617361 INFO @ Tue, 16 Jun 2020 10:43:13: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:43:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:43:14: #1 tags after filtering in treatment: 53617361 INFO @ Tue, 16 Jun 2020 10:43:14: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:43:14: #1 finished! INFO @ Tue, 16 Jun 2020 10:43:14: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:43:14: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:43:16: 49000000 INFO @ Tue, 16 Jun 2020 10:43:17: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 10:43:17: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:43:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7971812/SRX7971812.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971812/SRX7971812.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971812/SRX7971812.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971812/SRX7971812.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:43:22: 50000000 INFO @ Tue, 16 Jun 2020 10:43:28: 51000000 INFO @ Tue, 16 Jun 2020 10:43:34: 52000000 INFO @ Tue, 16 Jun 2020 10:43:40: 53000000 INFO @ Tue, 16 Jun 2020 10:43:44: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 10:43:44: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 10:43:44: #1 total tags in treatment: 53617361 INFO @ Tue, 16 Jun 2020 10:43:44: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:43:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:43:45: #1 tags after filtering in treatment: 53617361 INFO @ Tue, 16 Jun 2020 10:43:45: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:43:45: #1 finished! INFO @ Tue, 16 Jun 2020 10:43:45: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:43:45: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:43:48: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 10:43:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:43:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7971812/SRX7971812.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971812/SRX7971812.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971812/SRX7971812.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971812/SRX7971812.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。