Job ID = 6368986 SRX = SRX7971811 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-16T00:42:16 prefetch.2.10.7: 1) Downloading 'SRR11392697'... 2020-06-16T00:42:16 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:51:49 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:51:49 prefetch.2.10.7: 1) 'SRR11392697' was downloaded successfully Read 65033650 spots for SRR11392697/SRR11392697.sra Written 65033650 spots for SRR11392697/SRR11392697.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:24:18 65033650 reads; of these: 65033650 (100.00%) were unpaired; of these: 1650618 (2.54%) aligned 0 times 48058181 (73.90%) aligned exactly 1 time 15324851 (23.56%) aligned >1 times 97.46% overall alignment rate Time searching: 00:24:18 Overall time: 00:24:18 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 20364230 / 63383032 = 0.3213 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:36:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7971811/SRX7971811.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7971811/SRX7971811.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7971811/SRX7971811.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7971811/SRX7971811.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:36:05: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:36:05: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:36:14: 1000000 INFO @ Tue, 16 Jun 2020 10:36:23: 2000000 INFO @ Tue, 16 Jun 2020 10:36:32: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:36:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7971811/SRX7971811.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7971811/SRX7971811.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7971811/SRX7971811.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7971811/SRX7971811.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:36:35: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:36:35: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:36:42: 4000000 INFO @ Tue, 16 Jun 2020 10:36:44: 1000000 INFO @ Tue, 16 Jun 2020 10:36:51: 5000000 INFO @ Tue, 16 Jun 2020 10:36:53: 2000000 INFO @ Tue, 16 Jun 2020 10:37:01: 6000000 INFO @ Tue, 16 Jun 2020 10:37:02: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:37:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7971811/SRX7971811.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7971811/SRX7971811.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7971811/SRX7971811.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7971811/SRX7971811.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:37:05: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:37:05: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:37:11: 7000000 INFO @ Tue, 16 Jun 2020 10:37:11: 4000000 INFO @ Tue, 16 Jun 2020 10:37:14: 1000000 INFO @ Tue, 16 Jun 2020 10:37:21: 5000000 INFO @ Tue, 16 Jun 2020 10:37:22: 8000000 INFO @ Tue, 16 Jun 2020 10:37:24: 2000000 INFO @ Tue, 16 Jun 2020 10:37:30: 6000000 INFO @ Tue, 16 Jun 2020 10:37:32: 9000000 INFO @ Tue, 16 Jun 2020 10:37:33: 3000000 INFO @ Tue, 16 Jun 2020 10:37:39: 7000000 INFO @ Tue, 16 Jun 2020 10:37:42: 10000000 INFO @ Tue, 16 Jun 2020 10:37:42: 4000000 INFO @ Tue, 16 Jun 2020 10:37:49: 8000000 INFO @ Tue, 16 Jun 2020 10:37:51: 5000000 INFO @ Tue, 16 Jun 2020 10:37:52: 11000000 INFO @ Tue, 16 Jun 2020 10:37:58: 9000000 INFO @ Tue, 16 Jun 2020 10:38:01: 6000000 INFO @ Tue, 16 Jun 2020 10:38:02: 12000000 INFO @ Tue, 16 Jun 2020 10:38:07: 10000000 INFO @ Tue, 16 Jun 2020 10:38:10: 7000000 INFO @ Tue, 16 Jun 2020 10:38:12: 13000000 INFO @ Tue, 16 Jun 2020 10:38:16: 11000000 INFO @ Tue, 16 Jun 2020 10:38:19: 8000000 INFO @ Tue, 16 Jun 2020 10:38:22: 14000000 INFO @ Tue, 16 Jun 2020 10:38:25: 12000000 INFO @ Tue, 16 Jun 2020 10:38:28: 9000000 INFO @ Tue, 16 Jun 2020 10:38:32: 15000000 INFO @ Tue, 16 Jun 2020 10:38:34: 13000000 INFO @ Tue, 16 Jun 2020 10:38:37: 10000000 INFO @ Tue, 16 Jun 2020 10:38:42: 16000000 INFO @ Tue, 16 Jun 2020 10:38:43: 14000000 INFO @ Tue, 16 Jun 2020 10:38:46: 11000000 INFO @ Tue, 16 Jun 2020 10:38:52: 17000000 INFO @ Tue, 16 Jun 2020 10:38:52: 15000000 INFO @ Tue, 16 Jun 2020 10:38:55: 12000000 INFO @ Tue, 16 Jun 2020 10:39:01: 16000000 INFO @ Tue, 16 Jun 2020 10:39:02: 18000000 INFO @ Tue, 16 Jun 2020 10:39:04: 13000000 INFO @ Tue, 16 Jun 2020 10:39:10: 17000000 INFO @ Tue, 16 Jun 2020 10:39:12: 19000000 INFO @ Tue, 16 Jun 2020 10:39:13: 14000000 INFO @ Tue, 16 Jun 2020 10:39:19: 18000000 INFO @ Tue, 16 Jun 2020 10:39:22: 15000000 INFO @ Tue, 16 Jun 2020 10:39:22: 20000000 INFO @ Tue, 16 Jun 2020 10:39:28: 19000000 INFO @ Tue, 16 Jun 2020 10:39:31: 16000000 INFO @ Tue, 16 Jun 2020 10:39:32: 21000000 INFO @ Tue, 16 Jun 2020 10:39:37: 20000000 INFO @ Tue, 16 Jun 2020 10:39:40: 17000000 INFO @ Tue, 16 Jun 2020 10:39:42: 22000000 INFO @ Tue, 16 Jun 2020 10:39:46: 21000000 INFO @ Tue, 16 Jun 2020 10:39:48: 18000000 INFO @ Tue, 16 Jun 2020 10:39:52: 23000000 INFO @ Tue, 16 Jun 2020 10:39:55: 22000000 INFO @ Tue, 16 Jun 2020 10:39:57: 19000000 INFO @ Tue, 16 Jun 2020 10:40:02: 24000000 INFO @ Tue, 16 Jun 2020 10:40:04: 23000000 INFO @ Tue, 16 Jun 2020 10:40:06: 20000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 10:40:12: 25000000 INFO @ Tue, 16 Jun 2020 10:40:13: 24000000 INFO @ Tue, 16 Jun 2020 10:40:15: 21000000 INFO @ Tue, 16 Jun 2020 10:40:21: 26000000 INFO @ Tue, 16 Jun 2020 10:40:22: 25000000 INFO @ Tue, 16 Jun 2020 10:40:24: 22000000 INFO @ Tue, 16 Jun 2020 10:40:31: 26000000 INFO @ Tue, 16 Jun 2020 10:40:31: 27000000 INFO @ Tue, 16 Jun 2020 10:40:33: 23000000 INFO @ Tue, 16 Jun 2020 10:40:40: 27000000 INFO @ Tue, 16 Jun 2020 10:40:41: 28000000 INFO @ Tue, 16 Jun 2020 10:40:42: 24000000 INFO @ Tue, 16 Jun 2020 10:40:49: 28000000 INFO @ Tue, 16 Jun 2020 10:40:50: 29000000 INFO @ Tue, 16 Jun 2020 10:40:51: 25000000 INFO @ Tue, 16 Jun 2020 10:40:58: 29000000 INFO @ Tue, 16 Jun 2020 10:41:00: 30000000 INFO @ Tue, 16 Jun 2020 10:41:00: 26000000 INFO @ Tue, 16 Jun 2020 10:41:07: 30000000 INFO @ Tue, 16 Jun 2020 10:41:09: 27000000 INFO @ Tue, 16 Jun 2020 10:41:10: 31000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 10:41:16: 31000000 INFO @ Tue, 16 Jun 2020 10:41:19: 28000000 INFO @ Tue, 16 Jun 2020 10:41:20: 32000000 INFO @ Tue, 16 Jun 2020 10:41:25: 32000000 INFO @ Tue, 16 Jun 2020 10:41:28: 29000000 INFO @ Tue, 16 Jun 2020 10:41:29: 33000000 INFO @ Tue, 16 Jun 2020 10:41:34: 33000000 INFO @ Tue, 16 Jun 2020 10:41:37: 30000000 INFO @ Tue, 16 Jun 2020 10:41:39: 34000000 INFO @ Tue, 16 Jun 2020 10:41:43: 34000000 INFO @ Tue, 16 Jun 2020 10:41:46: 31000000 INFO @ Tue, 16 Jun 2020 10:41:49: 35000000 INFO @ Tue, 16 Jun 2020 10:41:52: 35000000 INFO @ Tue, 16 Jun 2020 10:41:55: 32000000 INFO @ Tue, 16 Jun 2020 10:41:59: 36000000 INFO @ Tue, 16 Jun 2020 10:42:01: 36000000 INFO @ Tue, 16 Jun 2020 10:42:04: 33000000 INFO @ Tue, 16 Jun 2020 10:42:09: 37000000 INFO @ Tue, 16 Jun 2020 10:42:10: 37000000 INFO @ Tue, 16 Jun 2020 10:42:12: 34000000 INFO @ Tue, 16 Jun 2020 10:42:19: 38000000 INFO @ Tue, 16 Jun 2020 10:42:20: 38000000 INFO @ Tue, 16 Jun 2020 10:42:21: 35000000 INFO @ Tue, 16 Jun 2020 10:42:30: 39000000 INFO @ Tue, 16 Jun 2020 10:42:30: 39000000 INFO @ Tue, 16 Jun 2020 10:42:30: 36000000 INFO @ Tue, 16 Jun 2020 10:42:39: 37000000 INFO @ Tue, 16 Jun 2020 10:42:40: 40000000 INFO @ Tue, 16 Jun 2020 10:42:41: 40000000 INFO @ Tue, 16 Jun 2020 10:42:48: 38000000 INFO @ Tue, 16 Jun 2020 10:42:51: 41000000 INFO @ Tue, 16 Jun 2020 10:42:52: 41000000 INFO @ Tue, 16 Jun 2020 10:42:57: 39000000 INFO @ Tue, 16 Jun 2020 10:43:02: 42000000 INFO @ Tue, 16 Jun 2020 10:43:02: 42000000 INFO @ Tue, 16 Jun 2020 10:43:06: 40000000 INFO @ Tue, 16 Jun 2020 10:43:11: 43000000 INFO @ Tue, 16 Jun 2020 10:43:11: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 10:43:11: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 10:43:11: #1 total tags in treatment: 43018802 INFO @ Tue, 16 Jun 2020 10:43:11: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:43:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:43:12: #1 tags after filtering in treatment: 43018802 INFO @ Tue, 16 Jun 2020 10:43:12: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:43:12: #1 finished! INFO @ Tue, 16 Jun 2020 10:43:12: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:43:12: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:43:12: 43000000 INFO @ Tue, 16 Jun 2020 10:43:13: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 10:43:13: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 10:43:13: #1 total tags in treatment: 43018802 INFO @ Tue, 16 Jun 2020 10:43:13: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:43:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:43:13: #1 tags after filtering in treatment: 43018802 INFO @ Tue, 16 Jun 2020 10:43:13: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:43:13: #1 finished! INFO @ Tue, 16 Jun 2020 10:43:13: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:43:13: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:43:15: 41000000 INFO @ Tue, 16 Jun 2020 10:43:15: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 10:43:15: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:43:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7971811/SRX7971811.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971811/SRX7971811.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971811/SRX7971811.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971811/SRX7971811.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:43:16: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 10:43:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:43:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7971811/SRX7971811.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971811/SRX7971811.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971811/SRX7971811.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971811/SRX7971811.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:43:22: 42000000 INFO @ Tue, 16 Jun 2020 10:43:29: 43000000 INFO @ Tue, 16 Jun 2020 10:43:30: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 10:43:30: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 10:43:30: #1 total tags in treatment: 43018802 INFO @ Tue, 16 Jun 2020 10:43:30: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:43:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:43:30: #1 tags after filtering in treatment: 43018802 INFO @ Tue, 16 Jun 2020 10:43:30: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:43:30: #1 finished! INFO @ Tue, 16 Jun 2020 10:43:30: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:43:30: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:43:33: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 10:43:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:43:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7971811/SRX7971811.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971811/SRX7971811.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971811/SRX7971811.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971811/SRX7971811.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling