Job ID = 6368980 SRX = SRX7971805 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-16T00:39:04 prefetch.2.10.7: 1) Downloading 'SRR11392691'... 2020-06-16T00:39:04 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:47:39 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:47:39 prefetch.2.10.7: 1) 'SRR11392691' was downloaded successfully Read 32524259 spots for SRR11392691/SRR11392691.sra Written 32524259 spots for SRR11392691/SRR11392691.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:17 32524259 reads; of these: 32524259 (100.00%) were unpaired; of these: 521999 (1.60%) aligned 0 times 28796632 (88.54%) aligned exactly 1 time 3205628 (9.86%) aligned >1 times 98.40% overall alignment rate Time searching: 00:10:18 Overall time: 00:10:18 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 6496576 / 32002260 = 0.2030 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:08:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7971805/SRX7971805.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7971805/SRX7971805.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7971805/SRX7971805.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7971805/SRX7971805.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:08:44: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:08:44: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:08:52: 1000000 INFO @ Tue, 16 Jun 2020 10:08:59: 2000000 INFO @ Tue, 16 Jun 2020 10:09:06: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:09:13: 4000000 INFO @ Tue, 16 Jun 2020 10:09:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7971805/SRX7971805.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7971805/SRX7971805.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7971805/SRX7971805.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7971805/SRX7971805.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:09:14: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:09:14: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:09:21: 5000000 INFO @ Tue, 16 Jun 2020 10:09:22: 1000000 INFO @ Tue, 16 Jun 2020 10:09:29: 6000000 INFO @ Tue, 16 Jun 2020 10:09:30: 2000000 INFO @ Tue, 16 Jun 2020 10:09:37: 7000000 INFO @ Tue, 16 Jun 2020 10:09:38: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:09:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7971805/SRX7971805.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7971805/SRX7971805.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7971805/SRX7971805.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7971805/SRX7971805.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:09:44: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:09:44: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:09:45: 8000000 INFO @ Tue, 16 Jun 2020 10:09:46: 4000000 INFO @ Tue, 16 Jun 2020 10:09:52: 1000000 INFO @ Tue, 16 Jun 2020 10:09:53: 9000000 INFO @ Tue, 16 Jun 2020 10:09:54: 5000000 INFO @ Tue, 16 Jun 2020 10:10:00: 2000000 INFO @ Tue, 16 Jun 2020 10:10:01: 10000000 INFO @ Tue, 16 Jun 2020 10:10:02: 6000000 INFO @ Tue, 16 Jun 2020 10:10:08: 3000000 INFO @ Tue, 16 Jun 2020 10:10:09: 11000000 INFO @ Tue, 16 Jun 2020 10:10:09: 7000000 INFO @ Tue, 16 Jun 2020 10:10:16: 4000000 INFO @ Tue, 16 Jun 2020 10:10:17: 12000000 INFO @ Tue, 16 Jun 2020 10:10:17: 8000000 INFO @ Tue, 16 Jun 2020 10:10:23: 5000000 INFO @ Tue, 16 Jun 2020 10:10:25: 13000000 INFO @ Tue, 16 Jun 2020 10:10:25: 9000000 INFO @ Tue, 16 Jun 2020 10:10:31: 6000000 INFO @ Tue, 16 Jun 2020 10:10:33: 14000000 INFO @ Tue, 16 Jun 2020 10:10:33: 10000000 INFO @ Tue, 16 Jun 2020 10:10:39: 7000000 INFO @ Tue, 16 Jun 2020 10:10:41: 11000000 INFO @ Tue, 16 Jun 2020 10:10:41: 15000000 INFO @ Tue, 16 Jun 2020 10:10:47: 8000000 INFO @ Tue, 16 Jun 2020 10:10:48: 12000000 INFO @ Tue, 16 Jun 2020 10:10:48: 16000000 INFO @ Tue, 16 Jun 2020 10:10:54: 9000000 INFO @ Tue, 16 Jun 2020 10:10:56: 13000000 INFO @ Tue, 16 Jun 2020 10:10:56: 17000000 INFO @ Tue, 16 Jun 2020 10:11:02: 10000000 INFO @ Tue, 16 Jun 2020 10:11:04: 14000000 INFO @ Tue, 16 Jun 2020 10:11:04: 18000000 INFO @ Tue, 16 Jun 2020 10:11:10: 11000000 INFO @ Tue, 16 Jun 2020 10:11:12: 15000000 INFO @ Tue, 16 Jun 2020 10:11:12: 19000000 INFO @ Tue, 16 Jun 2020 10:11:17: 12000000 INFO @ Tue, 16 Jun 2020 10:11:20: 16000000 INFO @ Tue, 16 Jun 2020 10:11:20: 20000000 INFO @ Tue, 16 Jun 2020 10:11:26: 13000000 INFO @ Tue, 16 Jun 2020 10:11:28: 17000000 INFO @ Tue, 16 Jun 2020 10:11:28: 21000000 INFO @ Tue, 16 Jun 2020 10:11:35: 14000000 INFO @ Tue, 16 Jun 2020 10:11:36: 18000000 INFO @ Tue, 16 Jun 2020 10:11:36: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 10:11:43: 15000000 INFO @ Tue, 16 Jun 2020 10:11:45: 19000000 INFO @ Tue, 16 Jun 2020 10:11:45: 23000000 INFO @ Tue, 16 Jun 2020 10:11:51: 16000000 INFO @ Tue, 16 Jun 2020 10:11:53: 20000000 INFO @ Tue, 16 Jun 2020 10:11:53: 24000000 INFO @ Tue, 16 Jun 2020 10:12:00: 17000000 INFO @ Tue, 16 Jun 2020 10:12:01: 21000000 INFO @ Tue, 16 Jun 2020 10:12:01: 25000000 INFO @ Tue, 16 Jun 2020 10:12:05: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 10:12:05: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 10:12:05: #1 total tags in treatment: 25505684 INFO @ Tue, 16 Jun 2020 10:12:05: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:12:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:12:06: #1 tags after filtering in treatment: 25505684 INFO @ Tue, 16 Jun 2020 10:12:06: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:12:06: #1 finished! INFO @ Tue, 16 Jun 2020 10:12:06: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:12:06: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:12:07: #2 number of paired peaks: 59 WARNING @ Tue, 16 Jun 2020 10:12:07: Too few paired peaks (59) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:12:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7971805/SRX7971805.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971805/SRX7971805.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971805/SRX7971805.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971805/SRX7971805.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:12:08: 18000000 INFO @ Tue, 16 Jun 2020 10:12:09: 22000000 INFO @ Tue, 16 Jun 2020 10:12:16: 19000000 INFO @ Tue, 16 Jun 2020 10:12:17: 23000000 INFO @ Tue, 16 Jun 2020 10:12:23: 20000000 INFO @ Tue, 16 Jun 2020 10:12:24: 24000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 10:12:31: 21000000 INFO @ Tue, 16 Jun 2020 10:12:32: 25000000 INFO @ Tue, 16 Jun 2020 10:12:36: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 10:12:36: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 10:12:36: #1 total tags in treatment: 25505684 INFO @ Tue, 16 Jun 2020 10:12:36: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:12:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:12:37: #1 tags after filtering in treatment: 25505684 INFO @ Tue, 16 Jun 2020 10:12:37: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:12:37: #1 finished! INFO @ Tue, 16 Jun 2020 10:12:37: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:12:37: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:12:38: #2 number of paired peaks: 59 WARNING @ Tue, 16 Jun 2020 10:12:38: Too few paired peaks (59) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:12:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7971805/SRX7971805.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971805/SRX7971805.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971805/SRX7971805.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971805/SRX7971805.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:12:39: 22000000 INFO @ Tue, 16 Jun 2020 10:12:46: 23000000 INFO @ Tue, 16 Jun 2020 10:12:53: 24000000 INFO @ Tue, 16 Jun 2020 10:13:00: 25000000 INFO @ Tue, 16 Jun 2020 10:13:03: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 10:13:03: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 10:13:03: #1 total tags in treatment: 25505684 INFO @ Tue, 16 Jun 2020 10:13:03: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:13:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:13:04: #1 tags after filtering in treatment: 25505684 INFO @ Tue, 16 Jun 2020 10:13:04: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:13:04: #1 finished! INFO @ Tue, 16 Jun 2020 10:13:04: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:13:04: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:13:05: #2 number of paired peaks: 59 WARNING @ Tue, 16 Jun 2020 10:13:05: Too few paired peaks (59) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:13:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7971805/SRX7971805.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971805/SRX7971805.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971805/SRX7971805.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7971805/SRX7971805.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling