Job ID = 10165931 SRX = SRX7879256 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:52 40235808 reads; of these: 40235808 (100.00%) were unpaired; of these: 401098 (1.00%) aligned 0 times 33826014 (84.07%) aligned exactly 1 time 6008696 (14.93%) aligned >1 times 99.00% overall alignment rate Time searching: 00:08:52 Overall time: 00:08:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 6784474 / 39834710 = 0.1703 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:29:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7879256/SRX7879256.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7879256/SRX7879256.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7879256/SRX7879256.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7879256/SRX7879256.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:29:35: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:29:35: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:29:40: 1000000 INFO @ Thu, 08 Oct 2020 20:29:46: 2000000 INFO @ Thu, 08 Oct 2020 20:29:51: 3000000 INFO @ Thu, 08 Oct 2020 20:29:57: 4000000 INFO @ Thu, 08 Oct 2020 20:30:02: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:30:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7879256/SRX7879256.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7879256/SRX7879256.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7879256/SRX7879256.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7879256/SRX7879256.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:30:06: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:30:06: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:30:08: 6000000 INFO @ Thu, 08 Oct 2020 20:30:13: 1000000 INFO @ Thu, 08 Oct 2020 20:30:15: 7000000 INFO @ Thu, 08 Oct 2020 20:30:19: 2000000 INFO @ Thu, 08 Oct 2020 20:30:21: 8000000 INFO @ Thu, 08 Oct 2020 20:30:25: 3000000 INFO @ Thu, 08 Oct 2020 20:30:27: 9000000 INFO @ Thu, 08 Oct 2020 20:30:31: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:30:33: 10000000 INFO @ Thu, 08 Oct 2020 20:30:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7879256/SRX7879256.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7879256/SRX7879256.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7879256/SRX7879256.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7879256/SRX7879256.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:30:36: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:30:36: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:30:38: 5000000 INFO @ Thu, 08 Oct 2020 20:30:39: 11000000 INFO @ Thu, 08 Oct 2020 20:30:42: 1000000 INFO @ Thu, 08 Oct 2020 20:30:44: 6000000 INFO @ Thu, 08 Oct 2020 20:30:46: 12000000 INFO @ Thu, 08 Oct 2020 20:30:48: 2000000 INFO @ Thu, 08 Oct 2020 20:30:51: 7000000 INFO @ Thu, 08 Oct 2020 20:30:52: 13000000 INFO @ Thu, 08 Oct 2020 20:30:55: 3000000 INFO @ Thu, 08 Oct 2020 20:30:57: 8000000 INFO @ Thu, 08 Oct 2020 20:30:58: 14000000 INFO @ Thu, 08 Oct 2020 20:31:01: 4000000 INFO @ Thu, 08 Oct 2020 20:31:03: 9000000 INFO @ Thu, 08 Oct 2020 20:31:04: 15000000 INFO @ Thu, 08 Oct 2020 20:31:07: 5000000 INFO @ Thu, 08 Oct 2020 20:31:09: 10000000 INFO @ Thu, 08 Oct 2020 20:31:10: 16000000 INFO @ Thu, 08 Oct 2020 20:31:13: 6000000 INFO @ Thu, 08 Oct 2020 20:31:15: 11000000 INFO @ Thu, 08 Oct 2020 20:31:16: 17000000 INFO @ Thu, 08 Oct 2020 20:31:19: 7000000 INFO @ Thu, 08 Oct 2020 20:31:21: 12000000 INFO @ Thu, 08 Oct 2020 20:31:22: 18000000 INFO @ Thu, 08 Oct 2020 20:31:25: 8000000 INFO @ Thu, 08 Oct 2020 20:31:27: 13000000 INFO @ Thu, 08 Oct 2020 20:31:28: 19000000 INFO @ Thu, 08 Oct 2020 20:31:32: 9000000 INFO @ Thu, 08 Oct 2020 20:31:33: 14000000 INFO @ Thu, 08 Oct 2020 20:31:34: 20000000 INFO @ Thu, 08 Oct 2020 20:31:38: 10000000 INFO @ Thu, 08 Oct 2020 20:31:39: 15000000 INFO @ Thu, 08 Oct 2020 20:31:40: 21000000 INFO @ Thu, 08 Oct 2020 20:31:44: 11000000 INFO @ Thu, 08 Oct 2020 20:31:45: 16000000 INFO @ Thu, 08 Oct 2020 20:31:46: 22000000 INFO @ Thu, 08 Oct 2020 20:31:50: 12000000 INFO @ Thu, 08 Oct 2020 20:31:51: 17000000 INFO @ Thu, 08 Oct 2020 20:31:52: 23000000 INFO @ Thu, 08 Oct 2020 20:31:56: 13000000 INFO @ Thu, 08 Oct 2020 20:31:57: 18000000 INFO @ Thu, 08 Oct 2020 20:31:58: 24000000 INFO @ Thu, 08 Oct 2020 20:32:02: 14000000 INFO @ Thu, 08 Oct 2020 20:32:03: 19000000 INFO @ Thu, 08 Oct 2020 20:32:04: 25000000 INFO @ Thu, 08 Oct 2020 20:32:08: 15000000 INFO @ Thu, 08 Oct 2020 20:32:09: 20000000 INFO @ Thu, 08 Oct 2020 20:32:10: 26000000 INFO @ Thu, 08 Oct 2020 20:32:14: 16000000 INFO @ Thu, 08 Oct 2020 20:32:16: 21000000 INFO @ Thu, 08 Oct 2020 20:32:16: 27000000 INFO @ Thu, 08 Oct 2020 20:32:20: 17000000 INFO @ Thu, 08 Oct 2020 20:32:22: 22000000 INFO @ Thu, 08 Oct 2020 20:32:23: 28000000 INFO @ Thu, 08 Oct 2020 20:32:27: 18000000 INFO @ Thu, 08 Oct 2020 20:32:28: 23000000 INFO @ Thu, 08 Oct 2020 20:32:29: 29000000 INFO @ Thu, 08 Oct 2020 20:32:33: 19000000 INFO @ Thu, 08 Oct 2020 20:32:34: 24000000 INFO @ Thu, 08 Oct 2020 20:32:35: 30000000 INFO @ Thu, 08 Oct 2020 20:32:39: 20000000 INFO @ Thu, 08 Oct 2020 20:32:40: 25000000 INFO @ Thu, 08 Oct 2020 20:32:41: 31000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 08 Oct 2020 20:32:45: 21000000 INFO @ Thu, 08 Oct 2020 20:32:46: 26000000 INFO @ Thu, 08 Oct 2020 20:32:47: 32000000 INFO @ Thu, 08 Oct 2020 20:32:51: 22000000 INFO @ Thu, 08 Oct 2020 20:32:52: 27000000 INFO @ Thu, 08 Oct 2020 20:32:53: 33000000 INFO @ Thu, 08 Oct 2020 20:32:54: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:32:54: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:32:54: #1 total tags in treatment: 33050236 INFO @ Thu, 08 Oct 2020 20:32:54: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:32:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:32:54: #1 tags after filtering in treatment: 33050236 INFO @ Thu, 08 Oct 2020 20:32:54: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:32:54: #1 finished! INFO @ Thu, 08 Oct 2020 20:32:54: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:32:54: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:32:56: #2 number of paired peaks: 19 WARNING @ Thu, 08 Oct 2020 20:32:56: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:32:56: Process for pairing-model is terminated! INFO @ Thu, 08 Oct 2020 20:32:57: 23000000 cut: /home/okishinya/chipatlas/results/ce11/SRX7879256/SRX7879256.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879256/SRX7879256.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879256/SRX7879256.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879256/SRX7879256.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 20:32:58: 28000000 INFO @ Thu, 08 Oct 2020 20:33:04: 24000000 INFO @ Thu, 08 Oct 2020 20:33:04: 29000000 INFO @ Thu, 08 Oct 2020 20:33:10: 25000000 INFO @ Thu, 08 Oct 2020 20:33:10: 30000000 INFO @ Thu, 08 Oct 2020 20:33:16: 26000000 INFO @ Thu, 08 Oct 2020 20:33:16: 31000000 INFO @ Thu, 08 Oct 2020 20:33:22: 27000000 INFO @ Thu, 08 Oct 2020 20:33:22: 32000000 INFO @ Thu, 08 Oct 2020 20:33:28: 33000000 INFO @ Thu, 08 Oct 2020 20:33:28: 28000000 INFO @ Thu, 08 Oct 2020 20:33:29: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:33:29: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:33:29: #1 total tags in treatment: 33050236 INFO @ Thu, 08 Oct 2020 20:33:29: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:33:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:33:29: #1 tags after filtering in treatment: 33050236 INFO @ Thu, 08 Oct 2020 20:33:29: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:33:29: #1 finished! INFO @ Thu, 08 Oct 2020 20:33:29: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:33:29: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:33:31: #2 number of paired peaks: 19 WARNING @ Thu, 08 Oct 2020 20:33:31: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:33:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7879256/SRX7879256.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879256/SRX7879256.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879256/SRX7879256.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879256/SRX7879256.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 20:33:34: 29000000 INFO @ Thu, 08 Oct 2020 20:33:40: 30000000 BigWig に変換しました。 INFO @ Thu, 08 Oct 2020 20:33:45: 31000000 INFO @ Thu, 08 Oct 2020 20:33:51: 32000000 INFO @ Thu, 08 Oct 2020 20:33:56: 33000000 INFO @ Thu, 08 Oct 2020 20:33:57: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:33:57: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:33:57: #1 total tags in treatment: 33050236 INFO @ Thu, 08 Oct 2020 20:33:57: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:33:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:33:57: #1 tags after filtering in treatment: 33050236 INFO @ Thu, 08 Oct 2020 20:33:57: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:33:57: #1 finished! INFO @ Thu, 08 Oct 2020 20:33:57: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:33:57: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:33:59: #2 number of paired peaks: 19 WARNING @ Thu, 08 Oct 2020 20:33:59: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:33:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7879256/SRX7879256.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879256/SRX7879256.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879256/SRX7879256.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879256/SRX7879256.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling