Job ID = 10165938 SRX = SRX7879255 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:03 42913454 reads; of these: 42913454 (100.00%) were unpaired; of these: 529224 (1.23%) aligned 0 times 35925910 (83.72%) aligned exactly 1 time 6458320 (15.05%) aligned >1 times 98.77% overall alignment rate Time searching: 00:10:03 Overall time: 00:10:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 7522313 / 42384230 = 0.1775 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:33:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7879255/SRX7879255.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7879255/SRX7879255.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7879255/SRX7879255.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7879255/SRX7879255.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:33:53: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:33:53: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:34:00: 1000000 INFO @ Thu, 08 Oct 2020 20:34:07: 2000000 INFO @ Thu, 08 Oct 2020 20:34:13: 3000000 INFO @ Thu, 08 Oct 2020 20:34:20: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:34:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7879255/SRX7879255.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7879255/SRX7879255.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7879255/SRX7879255.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7879255/SRX7879255.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:34:23: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:34:23: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:34:27: 5000000 INFO @ Thu, 08 Oct 2020 20:34:30: 1000000 INFO @ Thu, 08 Oct 2020 20:34:34: 6000000 INFO @ Thu, 08 Oct 2020 20:34:37: 2000000 INFO @ Thu, 08 Oct 2020 20:34:40: 7000000 INFO @ Thu, 08 Oct 2020 20:34:44: 3000000 INFO @ Thu, 08 Oct 2020 20:34:47: 8000000 INFO @ Thu, 08 Oct 2020 20:34:50: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:34:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7879255/SRX7879255.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7879255/SRX7879255.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7879255/SRX7879255.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7879255/SRX7879255.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:34:53: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:34:53: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:34:54: 9000000 INFO @ Thu, 08 Oct 2020 20:34:57: 5000000 INFO @ Thu, 08 Oct 2020 20:35:00: 1000000 INFO @ Thu, 08 Oct 2020 20:35:00: 10000000 INFO @ Thu, 08 Oct 2020 20:35:04: 6000000 INFO @ Thu, 08 Oct 2020 20:35:07: 11000000 INFO @ Thu, 08 Oct 2020 20:35:07: 2000000 INFO @ Thu, 08 Oct 2020 20:35:11: 7000000 INFO @ Thu, 08 Oct 2020 20:35:14: 12000000 INFO @ Thu, 08 Oct 2020 20:35:14: 3000000 INFO @ Thu, 08 Oct 2020 20:35:17: 8000000 INFO @ Thu, 08 Oct 2020 20:35:20: 13000000 INFO @ Thu, 08 Oct 2020 20:35:21: 4000000 INFO @ Thu, 08 Oct 2020 20:35:24: 9000000 INFO @ Thu, 08 Oct 2020 20:35:27: 14000000 INFO @ Thu, 08 Oct 2020 20:35:27: 5000000 INFO @ Thu, 08 Oct 2020 20:35:30: 10000000 INFO @ Thu, 08 Oct 2020 20:35:34: 15000000 INFO @ Thu, 08 Oct 2020 20:35:34: 6000000 INFO @ Thu, 08 Oct 2020 20:35:37: 11000000 INFO @ Thu, 08 Oct 2020 20:35:40: 16000000 INFO @ Thu, 08 Oct 2020 20:35:41: 7000000 INFO @ Thu, 08 Oct 2020 20:35:44: 12000000 INFO @ Thu, 08 Oct 2020 20:35:47: 17000000 INFO @ Thu, 08 Oct 2020 20:35:47: 8000000 INFO @ Thu, 08 Oct 2020 20:35:50: 13000000 INFO @ Thu, 08 Oct 2020 20:35:54: 18000000 INFO @ Thu, 08 Oct 2020 20:35:54: 9000000 INFO @ Thu, 08 Oct 2020 20:35:57: 14000000 INFO @ Thu, 08 Oct 2020 20:36:00: 19000000 INFO @ Thu, 08 Oct 2020 20:36:01: 10000000 INFO @ Thu, 08 Oct 2020 20:36:04: 15000000 INFO @ Thu, 08 Oct 2020 20:36:07: 20000000 INFO @ Thu, 08 Oct 2020 20:36:07: 11000000 INFO @ Thu, 08 Oct 2020 20:36:10: 16000000 INFO @ Thu, 08 Oct 2020 20:36:14: 21000000 INFO @ Thu, 08 Oct 2020 20:36:14: 12000000 INFO @ Thu, 08 Oct 2020 20:36:17: 17000000 INFO @ Thu, 08 Oct 2020 20:36:20: 22000000 INFO @ Thu, 08 Oct 2020 20:36:21: 13000000 INFO @ Thu, 08 Oct 2020 20:36:24: 18000000 INFO @ Thu, 08 Oct 2020 20:36:27: 23000000 INFO @ Thu, 08 Oct 2020 20:36:27: 14000000 INFO @ Thu, 08 Oct 2020 20:36:30: 19000000 INFO @ Thu, 08 Oct 2020 20:36:34: 24000000 INFO @ Thu, 08 Oct 2020 20:36:34: 15000000 INFO @ Thu, 08 Oct 2020 20:36:37: 20000000 INFO @ Thu, 08 Oct 2020 20:36:40: 25000000 INFO @ Thu, 08 Oct 2020 20:36:41: 16000000 INFO @ Thu, 08 Oct 2020 20:36:44: 21000000 INFO @ Thu, 08 Oct 2020 20:36:47: 26000000 INFO @ Thu, 08 Oct 2020 20:36:47: 17000000 INFO @ Thu, 08 Oct 2020 20:36:50: 22000000 INFO @ Thu, 08 Oct 2020 20:36:54: 27000000 INFO @ Thu, 08 Oct 2020 20:36:54: 18000000 INFO @ Thu, 08 Oct 2020 20:36:57: 23000000 INFO @ Thu, 08 Oct 2020 20:37:00: 28000000 INFO @ Thu, 08 Oct 2020 20:37:01: 19000000 INFO @ Thu, 08 Oct 2020 20:37:04: 24000000 INFO @ Thu, 08 Oct 2020 20:37:07: 29000000 INFO @ Thu, 08 Oct 2020 20:37:07: 20000000 INFO @ Thu, 08 Oct 2020 20:37:10: 25000000 INFO @ Thu, 08 Oct 2020 20:37:13: 30000000 INFO @ Thu, 08 Oct 2020 20:37:14: 21000000 INFO @ Thu, 08 Oct 2020 20:37:17: 26000000 INFO @ Thu, 08 Oct 2020 20:37:20: 31000000 INFO @ Thu, 08 Oct 2020 20:37:21: 22000000 INFO @ Thu, 08 Oct 2020 20:37:23: 27000000 INFO @ Thu, 08 Oct 2020 20:37:27: 32000000 INFO @ Thu, 08 Oct 2020 20:37:27: 23000000 INFO @ Thu, 08 Oct 2020 20:37:30: 28000000 INFO @ Thu, 08 Oct 2020 20:37:33: 33000000 INFO @ Thu, 08 Oct 2020 20:37:34: 24000000 INFO @ Thu, 08 Oct 2020 20:37:36: 29000000 INFO @ Thu, 08 Oct 2020 20:37:40: 34000000 INFO @ Thu, 08 Oct 2020 20:37:41: 25000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 08 Oct 2020 20:37:43: 30000000 INFO @ Thu, 08 Oct 2020 20:37:46: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:37:46: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:37:46: #1 total tags in treatment: 34861917 INFO @ Thu, 08 Oct 2020 20:37:46: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:37:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:37:46: #1 tags after filtering in treatment: 34861917 INFO @ Thu, 08 Oct 2020 20:37:46: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:37:46: #1 finished! INFO @ Thu, 08 Oct 2020 20:37:46: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:37:46: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:37:47: 26000000 INFO @ Thu, 08 Oct 2020 20:37:49: #2 number of paired peaks: 16 WARNING @ Thu, 08 Oct 2020 20:37:49: Too few paired peaks (16) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:37:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7879255/SRX7879255.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879255/SRX7879255.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879255/SRX7879255.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879255/SRX7879255.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 20:37:50: 31000000 INFO @ Thu, 08 Oct 2020 20:37:54: 27000000 INFO @ Thu, 08 Oct 2020 20:37:56: 32000000 INFO @ Thu, 08 Oct 2020 20:38:01: 28000000 INFO @ Thu, 08 Oct 2020 20:38:03: 33000000 INFO @ Thu, 08 Oct 2020 20:38:07: 29000000 INFO @ Thu, 08 Oct 2020 20:38:10: 34000000 INFO @ Thu, 08 Oct 2020 20:38:14: 30000000 INFO @ Thu, 08 Oct 2020 20:38:16: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:38:16: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:38:16: #1 total tags in treatment: 34861917 INFO @ Thu, 08 Oct 2020 20:38:16: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:38:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:38:17: #1 tags after filtering in treatment: 34861917 INFO @ Thu, 08 Oct 2020 20:38:17: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:38:17: #1 finished! INFO @ Thu, 08 Oct 2020 20:38:17: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:38:17: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:38:19: #2 number of paired peaks: 16 WARNING @ Thu, 08 Oct 2020 20:38:19: Too few paired peaks (16) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:38:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7879255/SRX7879255.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879255/SRX7879255.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879255/SRX7879255.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879255/SRX7879255.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 20:38:21: 31000000 INFO @ Thu, 08 Oct 2020 20:38:28: 32000000 INFO @ Thu, 08 Oct 2020 20:38:34: 33000000 INFO @ Thu, 08 Oct 2020 20:38:41: 34000000 INFO @ Thu, 08 Oct 2020 20:38:47: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:38:47: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:38:47: #1 total tags in treatment: 34861917 INFO @ Thu, 08 Oct 2020 20:38:47: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:38:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:38:48: #1 tags after filtering in treatment: 34861917 INFO @ Thu, 08 Oct 2020 20:38:48: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:38:48: #1 finished! INFO @ Thu, 08 Oct 2020 20:38:48: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:38:48: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:38:50: #2 number of paired peaks: 16 WARNING @ Thu, 08 Oct 2020 20:38:50: Too few paired peaks (16) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:38:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7879255/SRX7879255.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879255/SRX7879255.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879255/SRX7879255.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879255/SRX7879255.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。