Job ID = 10165930 SRX = SRX7879254 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:57 42721018 reads; of these: 42721018 (100.00%) were unpaired; of these: 521426 (1.22%) aligned 0 times 34419975 (80.57%) aligned exactly 1 time 7779617 (18.21%) aligned >1 times 98.78% overall alignment rate Time searching: 00:08:57 Overall time: 00:08:57 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 7899811 / 42199592 = 0.1872 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:29:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7879254/SRX7879254.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7879254/SRX7879254.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7879254/SRX7879254.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7879254/SRX7879254.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:29:15: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:29:15: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:29:22: 1000000 INFO @ Thu, 08 Oct 2020 20:29:27: 2000000 INFO @ Thu, 08 Oct 2020 20:29:33: 3000000 INFO @ Thu, 08 Oct 2020 20:29:39: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:29:45: 5000000 INFO @ Thu, 08 Oct 2020 20:29:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7879254/SRX7879254.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7879254/SRX7879254.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7879254/SRX7879254.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7879254/SRX7879254.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:29:45: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:29:45: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:29:51: 6000000 INFO @ Thu, 08 Oct 2020 20:29:52: 1000000 INFO @ Thu, 08 Oct 2020 20:29:58: 7000000 INFO @ Thu, 08 Oct 2020 20:29:59: 2000000 INFO @ Thu, 08 Oct 2020 20:30:04: 8000000 INFO @ Thu, 08 Oct 2020 20:30:05: 3000000 INFO @ Thu, 08 Oct 2020 20:30:10: 9000000 INFO @ Thu, 08 Oct 2020 20:30:12: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:30:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7879254/SRX7879254.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7879254/SRX7879254.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7879254/SRX7879254.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7879254/SRX7879254.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:30:15: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:30:15: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:30:17: 10000000 INFO @ Thu, 08 Oct 2020 20:30:18: 5000000 INFO @ Thu, 08 Oct 2020 20:30:23: 1000000 INFO @ Thu, 08 Oct 2020 20:30:24: 11000000 INFO @ Thu, 08 Oct 2020 20:30:26: 6000000 INFO @ Thu, 08 Oct 2020 20:30:31: 2000000 INFO @ Thu, 08 Oct 2020 20:30:32: 12000000 INFO @ Thu, 08 Oct 2020 20:30:33: 7000000 INFO @ Thu, 08 Oct 2020 20:30:39: 3000000 INFO @ Thu, 08 Oct 2020 20:30:39: 13000000 INFO @ Thu, 08 Oct 2020 20:30:41: 8000000 INFO @ Thu, 08 Oct 2020 20:30:46: 4000000 INFO @ Thu, 08 Oct 2020 20:30:47: 14000000 INFO @ Thu, 08 Oct 2020 20:30:48: 9000000 INFO @ Thu, 08 Oct 2020 20:30:54: 15000000 INFO @ Thu, 08 Oct 2020 20:30:54: 5000000 INFO @ Thu, 08 Oct 2020 20:30:56: 10000000 INFO @ Thu, 08 Oct 2020 20:31:01: 16000000 INFO @ Thu, 08 Oct 2020 20:31:02: 6000000 INFO @ Thu, 08 Oct 2020 20:31:03: 11000000 INFO @ Thu, 08 Oct 2020 20:31:09: 17000000 INFO @ Thu, 08 Oct 2020 20:31:10: 7000000 INFO @ Thu, 08 Oct 2020 20:31:10: 12000000 INFO @ Thu, 08 Oct 2020 20:31:16: 18000000 INFO @ Thu, 08 Oct 2020 20:31:18: 13000000 INFO @ Thu, 08 Oct 2020 20:31:18: 8000000 INFO @ Thu, 08 Oct 2020 20:31:24: 19000000 INFO @ Thu, 08 Oct 2020 20:31:25: 14000000 INFO @ Thu, 08 Oct 2020 20:31:26: 9000000 INFO @ Thu, 08 Oct 2020 20:31:31: 20000000 INFO @ Thu, 08 Oct 2020 20:31:32: 15000000 INFO @ Thu, 08 Oct 2020 20:31:34: 10000000 INFO @ Thu, 08 Oct 2020 20:31:38: 21000000 INFO @ Thu, 08 Oct 2020 20:31:40: 16000000 INFO @ Thu, 08 Oct 2020 20:31:41: 11000000 INFO @ Thu, 08 Oct 2020 20:31:46: 22000000 INFO @ Thu, 08 Oct 2020 20:31:47: 17000000 INFO @ Thu, 08 Oct 2020 20:31:49: 12000000 INFO @ Thu, 08 Oct 2020 20:31:53: 23000000 INFO @ Thu, 08 Oct 2020 20:31:54: 18000000 INFO @ Thu, 08 Oct 2020 20:31:57: 13000000 INFO @ Thu, 08 Oct 2020 20:32:00: 24000000 INFO @ Thu, 08 Oct 2020 20:32:02: 19000000 INFO @ Thu, 08 Oct 2020 20:32:05: 14000000 INFO @ Thu, 08 Oct 2020 20:32:08: 25000000 INFO @ Thu, 08 Oct 2020 20:32:09: 20000000 INFO @ Thu, 08 Oct 2020 20:32:13: 15000000 INFO @ Thu, 08 Oct 2020 20:32:15: 26000000 INFO @ Thu, 08 Oct 2020 20:32:16: 21000000 INFO @ Thu, 08 Oct 2020 20:32:20: 16000000 INFO @ Thu, 08 Oct 2020 20:32:22: 27000000 INFO @ Thu, 08 Oct 2020 20:32:24: 22000000 INFO @ Thu, 08 Oct 2020 20:32:28: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 08 Oct 2020 20:32:30: 28000000 INFO @ Thu, 08 Oct 2020 20:32:31: 23000000 INFO @ Thu, 08 Oct 2020 20:32:36: 18000000 INFO @ Thu, 08 Oct 2020 20:32:37: 29000000 INFO @ Thu, 08 Oct 2020 20:32:39: 24000000 INFO @ Thu, 08 Oct 2020 20:32:43: 19000000 INFO @ Thu, 08 Oct 2020 20:32:45: 30000000 INFO @ Thu, 08 Oct 2020 20:32:46: 25000000 INFO @ Thu, 08 Oct 2020 20:32:51: 20000000 INFO @ Thu, 08 Oct 2020 20:32:52: 31000000 INFO @ Thu, 08 Oct 2020 20:32:54: 26000000 INFO @ Thu, 08 Oct 2020 20:32:59: 21000000 INFO @ Thu, 08 Oct 2020 20:33:00: 32000000 INFO @ Thu, 08 Oct 2020 20:33:01: 27000000 INFO @ Thu, 08 Oct 2020 20:33:06: 22000000 INFO @ Thu, 08 Oct 2020 20:33:07: 33000000 INFO @ Thu, 08 Oct 2020 20:33:08: 28000000 INFO @ Thu, 08 Oct 2020 20:33:14: 23000000 INFO @ Thu, 08 Oct 2020 20:33:15: 34000000 INFO @ Thu, 08 Oct 2020 20:33:16: 29000000 INFO @ Thu, 08 Oct 2020 20:33:17: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:33:17: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:33:17: #1 total tags in treatment: 34299781 INFO @ Thu, 08 Oct 2020 20:33:17: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:33:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:33:17: #1 tags after filtering in treatment: 34299781 INFO @ Thu, 08 Oct 2020 20:33:17: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:33:17: #1 finished! INFO @ Thu, 08 Oct 2020 20:33:17: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:33:17: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:33:19: #2 number of paired peaks: 24 WARNING @ Thu, 08 Oct 2020 20:33:19: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:33:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7879254/SRX7879254.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879254/SRX7879254.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879254/SRX7879254.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879254/SRX7879254.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 20:33:22: 24000000 INFO @ Thu, 08 Oct 2020 20:33:23: 30000000 INFO @ Thu, 08 Oct 2020 20:33:29: 25000000 INFO @ Thu, 08 Oct 2020 20:33:30: 31000000 BigWig に変換しました。 INFO @ Thu, 08 Oct 2020 20:33:37: 26000000 INFO @ Thu, 08 Oct 2020 20:33:37: 32000000 INFO @ Thu, 08 Oct 2020 20:33:44: 33000000 INFO @ Thu, 08 Oct 2020 20:33:45: 27000000 INFO @ Thu, 08 Oct 2020 20:33:51: 34000000 INFO @ Thu, 08 Oct 2020 20:33:52: 28000000 INFO @ Thu, 08 Oct 2020 20:33:54: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:33:54: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:33:54: #1 total tags in treatment: 34299781 INFO @ Thu, 08 Oct 2020 20:33:54: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:33:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:33:54: #1 tags after filtering in treatment: 34299781 INFO @ Thu, 08 Oct 2020 20:33:54: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:33:54: #1 finished! INFO @ Thu, 08 Oct 2020 20:33:54: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:33:54: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:33:56: #2 number of paired peaks: 24 WARNING @ Thu, 08 Oct 2020 20:33:56: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:33:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7879254/SRX7879254.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879254/SRX7879254.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879254/SRX7879254.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879254/SRX7879254.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 20:33:59: 29000000 INFO @ Thu, 08 Oct 2020 20:34:06: 30000000 INFO @ Thu, 08 Oct 2020 20:34:13: 31000000 INFO @ Thu, 08 Oct 2020 20:34:20: 32000000 INFO @ Thu, 08 Oct 2020 20:34:27: 33000000 INFO @ Thu, 08 Oct 2020 20:34:34: 34000000 INFO @ Thu, 08 Oct 2020 20:34:36: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:34:36: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:34:36: #1 total tags in treatment: 34299781 INFO @ Thu, 08 Oct 2020 20:34:36: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:34:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:34:37: #1 tags after filtering in treatment: 34299781 INFO @ Thu, 08 Oct 2020 20:34:37: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:34:37: #1 finished! INFO @ Thu, 08 Oct 2020 20:34:37: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:34:37: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:34:39: #2 number of paired peaks: 24 WARNING @ Thu, 08 Oct 2020 20:34:39: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:34:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7879254/SRX7879254.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879254/SRX7879254.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879254/SRX7879254.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879254/SRX7879254.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling