Job ID = 10165992 SRX = SRX7879252 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:55 60530886 reads; of these: 60530886 (100.00%) were unpaired; of these: 571521 (0.94%) aligned 0 times 48958660 (80.88%) aligned exactly 1 time 11000705 (18.17%) aligned >1 times 99.06% overall alignment rate Time searching: 00:13:55 Overall time: 00:13:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 13923612 / 59959365 = 0.2322 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:50:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7879252/SRX7879252.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7879252/SRX7879252.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7879252/SRX7879252.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7879252/SRX7879252.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:50:00: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:50:00: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:50:05: 1000000 INFO @ Thu, 08 Oct 2020 20:50:11: 2000000 INFO @ Thu, 08 Oct 2020 20:50:17: 3000000 INFO @ Thu, 08 Oct 2020 20:50:23: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:50:29: 5000000 INFO @ Thu, 08 Oct 2020 20:50:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7879252/SRX7879252.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7879252/SRX7879252.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7879252/SRX7879252.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7879252/SRX7879252.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:50:29: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:50:29: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:50:35: 6000000 INFO @ Thu, 08 Oct 2020 20:50:36: 1000000 INFO @ Thu, 08 Oct 2020 20:50:42: 7000000 INFO @ Thu, 08 Oct 2020 20:50:43: 2000000 INFO @ Thu, 08 Oct 2020 20:50:49: 8000000 INFO @ Thu, 08 Oct 2020 20:50:50: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:50:56: 9000000 INFO @ Thu, 08 Oct 2020 20:50:57: 4000000 INFO @ Thu, 08 Oct 2020 20:51:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7879252/SRX7879252.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7879252/SRX7879252.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7879252/SRX7879252.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7879252/SRX7879252.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:51:00: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:51:00: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:51:02: 10000000 INFO @ Thu, 08 Oct 2020 20:51:04: 5000000 INFO @ Thu, 08 Oct 2020 20:51:07: 1000000 INFO @ Thu, 08 Oct 2020 20:51:09: 11000000 INFO @ Thu, 08 Oct 2020 20:51:12: 6000000 INFO @ Thu, 08 Oct 2020 20:51:14: 2000000 INFO @ Thu, 08 Oct 2020 20:51:16: 12000000 INFO @ Thu, 08 Oct 2020 20:51:19: 7000000 INFO @ Thu, 08 Oct 2020 20:51:21: 3000000 INFO @ Thu, 08 Oct 2020 20:51:23: 13000000 INFO @ Thu, 08 Oct 2020 20:51:26: 8000000 INFO @ Thu, 08 Oct 2020 20:51:29: 4000000 INFO @ Thu, 08 Oct 2020 20:51:30: 14000000 INFO @ Thu, 08 Oct 2020 20:51:33: 9000000 INFO @ Thu, 08 Oct 2020 20:51:36: 5000000 INFO @ Thu, 08 Oct 2020 20:51:37: 15000000 INFO @ Thu, 08 Oct 2020 20:51:40: 10000000 INFO @ Thu, 08 Oct 2020 20:51:43: 6000000 INFO @ Thu, 08 Oct 2020 20:51:43: 16000000 INFO @ Thu, 08 Oct 2020 20:51:47: 11000000 INFO @ Thu, 08 Oct 2020 20:51:50: 7000000 INFO @ Thu, 08 Oct 2020 20:51:50: 17000000 INFO @ Thu, 08 Oct 2020 20:51:55: 12000000 INFO @ Thu, 08 Oct 2020 20:51:57: 18000000 INFO @ Thu, 08 Oct 2020 20:51:57: 8000000 INFO @ Thu, 08 Oct 2020 20:52:02: 13000000 INFO @ Thu, 08 Oct 2020 20:52:04: 19000000 INFO @ Thu, 08 Oct 2020 20:52:05: 9000000 INFO @ Thu, 08 Oct 2020 20:52:09: 14000000 INFO @ Thu, 08 Oct 2020 20:52:11: 20000000 INFO @ Thu, 08 Oct 2020 20:52:12: 10000000 INFO @ Thu, 08 Oct 2020 20:52:16: 15000000 INFO @ Thu, 08 Oct 2020 20:52:18: 21000000 INFO @ Thu, 08 Oct 2020 20:52:19: 11000000 INFO @ Thu, 08 Oct 2020 20:52:23: 16000000 INFO @ Thu, 08 Oct 2020 20:52:25: 22000000 INFO @ Thu, 08 Oct 2020 20:52:26: 12000000 INFO @ Thu, 08 Oct 2020 20:52:30: 17000000 INFO @ Thu, 08 Oct 2020 20:52:32: 23000000 INFO @ Thu, 08 Oct 2020 20:52:33: 13000000 INFO @ Thu, 08 Oct 2020 20:52:37: 18000000 INFO @ Thu, 08 Oct 2020 20:52:38: 24000000 INFO @ Thu, 08 Oct 2020 20:52:40: 14000000 INFO @ Thu, 08 Oct 2020 20:52:44: 19000000 INFO @ Thu, 08 Oct 2020 20:52:45: 25000000 INFO @ Thu, 08 Oct 2020 20:52:47: 15000000 INFO @ Thu, 08 Oct 2020 20:52:51: 20000000 INFO @ Thu, 08 Oct 2020 20:52:52: 26000000 INFO @ Thu, 08 Oct 2020 20:52:54: 16000000 INFO @ Thu, 08 Oct 2020 20:52:58: 21000000 INFO @ Thu, 08 Oct 2020 20:52:59: 27000000 INFO @ Thu, 08 Oct 2020 20:53:02: 17000000 INFO @ Thu, 08 Oct 2020 20:53:05: 22000000 INFO @ Thu, 08 Oct 2020 20:53:06: 28000000 INFO @ Thu, 08 Oct 2020 20:53:09: 18000000 INFO @ Thu, 08 Oct 2020 20:53:13: 23000000 INFO @ Thu, 08 Oct 2020 20:53:13: 29000000 INFO @ Thu, 08 Oct 2020 20:53:16: 19000000 INFO @ Thu, 08 Oct 2020 20:53:19: 30000000 INFO @ Thu, 08 Oct 2020 20:53:20: 24000000 INFO @ Thu, 08 Oct 2020 20:53:23: 20000000 INFO @ Thu, 08 Oct 2020 20:53:26: 31000000 INFO @ Thu, 08 Oct 2020 20:53:27: 25000000 INFO @ Thu, 08 Oct 2020 20:53:30: 21000000 INFO @ Thu, 08 Oct 2020 20:53:33: 32000000 INFO @ Thu, 08 Oct 2020 20:53:34: 26000000 INFO @ Thu, 08 Oct 2020 20:53:37: 22000000 INFO @ Thu, 08 Oct 2020 20:53:40: 33000000 INFO @ Thu, 08 Oct 2020 20:53:41: 27000000 INFO @ Thu, 08 Oct 2020 20:53:44: 23000000 INFO @ Thu, 08 Oct 2020 20:53:47: 34000000 INFO @ Thu, 08 Oct 2020 20:53:48: 28000000 INFO @ Thu, 08 Oct 2020 20:53:51: 24000000 INFO @ Thu, 08 Oct 2020 20:53:53: 35000000 INFO @ Thu, 08 Oct 2020 20:53:55: 29000000 INFO @ Thu, 08 Oct 2020 20:53:59: 25000000 INFO @ Thu, 08 Oct 2020 20:54:00: 36000000 INFO @ Thu, 08 Oct 2020 20:54:02: 30000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 08 Oct 2020 20:54:06: 26000000 INFO @ Thu, 08 Oct 2020 20:54:07: 37000000 INFO @ Thu, 08 Oct 2020 20:54:09: 31000000 INFO @ Thu, 08 Oct 2020 20:54:13: 27000000 INFO @ Thu, 08 Oct 2020 20:54:14: 38000000 INFO @ Thu, 08 Oct 2020 20:54:16: 32000000 INFO @ Thu, 08 Oct 2020 20:54:20: 28000000 INFO @ Thu, 08 Oct 2020 20:54:21: 39000000 INFO @ Thu, 08 Oct 2020 20:54:24: 33000000 INFO @ Thu, 08 Oct 2020 20:54:26: 29000000 INFO @ Thu, 08 Oct 2020 20:54:28: 40000000 INFO @ Thu, 08 Oct 2020 20:54:31: 34000000 INFO @ Thu, 08 Oct 2020 20:54:33: 30000000 INFO @ Thu, 08 Oct 2020 20:54:35: 41000000 INFO @ Thu, 08 Oct 2020 20:54:38: 35000000 INFO @ Thu, 08 Oct 2020 20:54:40: 31000000 INFO @ Thu, 08 Oct 2020 20:54:42: 42000000 INFO @ Thu, 08 Oct 2020 20:54:45: 36000000 INFO @ Thu, 08 Oct 2020 20:54:47: 32000000 INFO @ Thu, 08 Oct 2020 20:54:48: 43000000 INFO @ Thu, 08 Oct 2020 20:54:52: 37000000 INFO @ Thu, 08 Oct 2020 20:54:54: 33000000 INFO @ Thu, 08 Oct 2020 20:54:55: 44000000 INFO @ Thu, 08 Oct 2020 20:54:59: 38000000 INFO @ Thu, 08 Oct 2020 20:55:01: 34000000 INFO @ Thu, 08 Oct 2020 20:55:02: 45000000 INFO @ Thu, 08 Oct 2020 20:55:07: 39000000 INFO @ Thu, 08 Oct 2020 20:55:08: 35000000 INFO @ Thu, 08 Oct 2020 20:55:09: 46000000 INFO @ Thu, 08 Oct 2020 20:55:09: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:55:09: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:55:09: #1 total tags in treatment: 46035753 INFO @ Thu, 08 Oct 2020 20:55:09: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:55:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:55:10: #1 tags after filtering in treatment: 46035753 INFO @ Thu, 08 Oct 2020 20:55:10: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:55:10: #1 finished! INFO @ Thu, 08 Oct 2020 20:55:10: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:55:10: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:55:13: #2 number of paired peaks: 0 WARNING @ Thu, 08 Oct 2020 20:55:13: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:55:13: Process for pairing-model is terminated! INFO @ Thu, 08 Oct 2020 20:55:14: 40000000 INFO @ Thu, 08 Oct 2020 20:55:15: 36000000 BigWig に変換しました。 cut: /home/okishinya/chipatlas/results/ce11/SRX7879252/SRX7879252.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879252/SRX7879252.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879252/SRX7879252.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879252/SRX7879252.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 20:55:21: 41000000 INFO @ Thu, 08 Oct 2020 20:55:22: 37000000 INFO @ Thu, 08 Oct 2020 20:55:28: 42000000 INFO @ Thu, 08 Oct 2020 20:55:29: 38000000 INFO @ Thu, 08 Oct 2020 20:55:35: 43000000 INFO @ Thu, 08 Oct 2020 20:55:36: 39000000 INFO @ Thu, 08 Oct 2020 20:55:42: 44000000 INFO @ Thu, 08 Oct 2020 20:55:43: 40000000 INFO @ Thu, 08 Oct 2020 20:55:49: 45000000 INFO @ Thu, 08 Oct 2020 20:55:50: 41000000 INFO @ Thu, 08 Oct 2020 20:55:56: 46000000 INFO @ Thu, 08 Oct 2020 20:55:56: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:55:56: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:55:56: #1 total tags in treatment: 46035753 INFO @ Thu, 08 Oct 2020 20:55:56: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:55:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:55:57: 42000000 INFO @ Thu, 08 Oct 2020 20:55:57: #1 tags after filtering in treatment: 46035753 INFO @ Thu, 08 Oct 2020 20:55:57: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:55:57: #1 finished! INFO @ Thu, 08 Oct 2020 20:55:57: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:55:57: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:56:00: #2 number of paired peaks: 0 WARNING @ Thu, 08 Oct 2020 20:56:00: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:56:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7879252/SRX7879252.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879252/SRX7879252.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879252/SRX7879252.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879252/SRX7879252.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 20:56:03: 43000000 INFO @ Thu, 08 Oct 2020 20:56:10: 44000000 INFO @ Thu, 08 Oct 2020 20:56:17: 45000000 INFO @ Thu, 08 Oct 2020 20:56:23: 46000000 INFO @ Thu, 08 Oct 2020 20:56:23: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:56:23: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:56:23: #1 total tags in treatment: 46035753 INFO @ Thu, 08 Oct 2020 20:56:23: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:56:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:56:24: #1 tags after filtering in treatment: 46035753 INFO @ Thu, 08 Oct 2020 20:56:24: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:56:24: #1 finished! INFO @ Thu, 08 Oct 2020 20:56:24: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:56:24: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:56:27: #2 number of paired peaks: 0 WARNING @ Thu, 08 Oct 2020 20:56:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:56:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7879252/SRX7879252.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879252/SRX7879252.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879252/SRX7879252.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879252/SRX7879252.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling