Job ID = 10165940 SRX = SRX7879251 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:25 41940504 reads; of these: 41940504 (100.00%) were unpaired; of these: 611416 (1.46%) aligned 0 times 33843770 (80.69%) aligned exactly 1 time 7485318 (17.85%) aligned >1 times 98.54% overall alignment rate Time searching: 00:10:25 Overall time: 00:10:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 7442167 / 41329088 = 0.1801 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:31:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7879251/SRX7879251.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7879251/SRX7879251.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7879251/SRX7879251.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7879251/SRX7879251.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:31:57: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:31:57: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:32:02: 1000000 INFO @ Thu, 08 Oct 2020 20:32:07: 2000000 INFO @ Thu, 08 Oct 2020 20:32:13: 3000000 INFO @ Thu, 08 Oct 2020 20:32:18: 4000000 INFO @ Thu, 08 Oct 2020 20:32:24: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:32:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7879251/SRX7879251.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7879251/SRX7879251.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7879251/SRX7879251.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7879251/SRX7879251.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:32:26: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:32:26: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:32:30: 6000000 INFO @ Thu, 08 Oct 2020 20:32:33: 1000000 INFO @ Thu, 08 Oct 2020 20:32:36: 7000000 INFO @ Thu, 08 Oct 2020 20:32:39: 2000000 INFO @ Thu, 08 Oct 2020 20:32:42: 8000000 INFO @ Thu, 08 Oct 2020 20:32:45: 3000000 INFO @ Thu, 08 Oct 2020 20:32:48: 9000000 INFO @ Thu, 08 Oct 2020 20:32:51: 4000000 INFO @ Thu, 08 Oct 2020 20:32:54: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:32:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7879251/SRX7879251.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7879251/SRX7879251.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7879251/SRX7879251.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7879251/SRX7879251.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:32:57: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:32:57: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:32:57: 5000000 INFO @ Thu, 08 Oct 2020 20:33:00: 11000000 INFO @ Thu, 08 Oct 2020 20:33:03: 1000000 INFO @ Thu, 08 Oct 2020 20:33:03: 6000000 INFO @ Thu, 08 Oct 2020 20:33:06: 12000000 INFO @ Thu, 08 Oct 2020 20:33:09: 2000000 INFO @ Thu, 08 Oct 2020 20:33:09: 7000000 INFO @ Thu, 08 Oct 2020 20:33:12: 13000000 INFO @ Thu, 08 Oct 2020 20:33:15: 3000000 INFO @ Thu, 08 Oct 2020 20:33:15: 8000000 INFO @ Thu, 08 Oct 2020 20:33:18: 14000000 INFO @ Thu, 08 Oct 2020 20:33:21: 9000000 INFO @ Thu, 08 Oct 2020 20:33:21: 4000000 INFO @ Thu, 08 Oct 2020 20:33:24: 15000000 INFO @ Thu, 08 Oct 2020 20:33:28: 10000000 INFO @ Thu, 08 Oct 2020 20:33:28: 5000000 INFO @ Thu, 08 Oct 2020 20:33:30: 16000000 INFO @ Thu, 08 Oct 2020 20:33:34: 11000000 INFO @ Thu, 08 Oct 2020 20:33:34: 6000000 INFO @ Thu, 08 Oct 2020 20:33:35: 17000000 INFO @ Thu, 08 Oct 2020 20:33:40: 7000000 INFO @ Thu, 08 Oct 2020 20:33:40: 12000000 INFO @ Thu, 08 Oct 2020 20:33:41: 18000000 INFO @ Thu, 08 Oct 2020 20:33:46: 8000000 INFO @ Thu, 08 Oct 2020 20:33:46: 13000000 INFO @ Thu, 08 Oct 2020 20:33:47: 19000000 INFO @ Thu, 08 Oct 2020 20:33:52: 9000000 INFO @ Thu, 08 Oct 2020 20:33:52: 14000000 INFO @ Thu, 08 Oct 2020 20:33:53: 20000000 INFO @ Thu, 08 Oct 2020 20:33:58: 10000000 INFO @ Thu, 08 Oct 2020 20:33:58: 15000000 INFO @ Thu, 08 Oct 2020 20:33:59: 21000000 INFO @ Thu, 08 Oct 2020 20:34:04: 11000000 INFO @ Thu, 08 Oct 2020 20:34:04: 16000000 INFO @ Thu, 08 Oct 2020 20:34:05: 22000000 INFO @ Thu, 08 Oct 2020 20:34:10: 12000000 INFO @ Thu, 08 Oct 2020 20:34:10: 17000000 INFO @ Thu, 08 Oct 2020 20:34:11: 23000000 INFO @ Thu, 08 Oct 2020 20:34:16: 13000000 INFO @ Thu, 08 Oct 2020 20:34:16: 18000000 INFO @ Thu, 08 Oct 2020 20:34:17: 24000000 INFO @ Thu, 08 Oct 2020 20:34:22: 14000000 INFO @ Thu, 08 Oct 2020 20:34:22: 19000000 INFO @ Thu, 08 Oct 2020 20:34:23: 25000000 INFO @ Thu, 08 Oct 2020 20:34:28: 15000000 INFO @ Thu, 08 Oct 2020 20:34:28: 20000000 INFO @ Thu, 08 Oct 2020 20:34:29: 26000000 INFO @ Thu, 08 Oct 2020 20:34:34: 16000000 INFO @ Thu, 08 Oct 2020 20:34:35: 21000000 INFO @ Thu, 08 Oct 2020 20:34:35: 27000000 INFO @ Thu, 08 Oct 2020 20:34:40: 17000000 INFO @ Thu, 08 Oct 2020 20:34:41: 22000000 INFO @ Thu, 08 Oct 2020 20:34:41: 28000000 INFO @ Thu, 08 Oct 2020 20:34:46: 29000000 INFO @ Thu, 08 Oct 2020 20:34:47: 18000000 INFO @ Thu, 08 Oct 2020 20:34:47: 23000000 INFO @ Thu, 08 Oct 2020 20:34:52: 30000000 INFO @ Thu, 08 Oct 2020 20:34:53: 19000000 INFO @ Thu, 08 Oct 2020 20:34:53: 24000000 INFO @ Thu, 08 Oct 2020 20:34:58: 31000000 INFO @ Thu, 08 Oct 2020 20:34:59: 20000000 INFO @ Thu, 08 Oct 2020 20:34:59: 25000000 INFO @ Thu, 08 Oct 2020 20:35:04: 32000000 INFO @ Thu, 08 Oct 2020 20:35:05: 21000000 INFO @ Thu, 08 Oct 2020 20:35:05: 26000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 08 Oct 2020 20:35:10: 33000000 INFO @ Thu, 08 Oct 2020 20:35:11: 22000000 INFO @ Thu, 08 Oct 2020 20:35:11: 27000000 INFO @ Thu, 08 Oct 2020 20:35:15: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:35:15: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:35:15: #1 total tags in treatment: 33886921 INFO @ Thu, 08 Oct 2020 20:35:15: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:35:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:35:16: #1 tags after filtering in treatment: 33886921 INFO @ Thu, 08 Oct 2020 20:35:16: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:35:16: #1 finished! INFO @ Thu, 08 Oct 2020 20:35:16: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:35:16: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:35:17: 28000000 INFO @ Thu, 08 Oct 2020 20:35:17: 23000000 INFO @ Thu, 08 Oct 2020 20:35:18: #2 number of paired peaks: 33 WARNING @ Thu, 08 Oct 2020 20:35:18: Too few paired peaks (33) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:35:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7879251/SRX7879251.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879251/SRX7879251.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879251/SRX7879251.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879251/SRX7879251.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 20:35:22: 29000000 INFO @ Thu, 08 Oct 2020 20:35:23: 24000000 INFO @ Thu, 08 Oct 2020 20:35:28: 30000000 INFO @ Thu, 08 Oct 2020 20:35:29: 25000000 INFO @ Thu, 08 Oct 2020 20:35:34: 31000000 INFO @ Thu, 08 Oct 2020 20:35:35: 26000000 INFO @ Thu, 08 Oct 2020 20:35:40: 32000000 INFO @ Thu, 08 Oct 2020 20:35:41: 27000000 INFO @ Thu, 08 Oct 2020 20:35:46: 33000000 INFO @ Thu, 08 Oct 2020 20:35:46: 28000000 INFO @ Thu, 08 Oct 2020 20:35:52: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:35:52: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:35:52: #1 total tags in treatment: 33886921 INFO @ Thu, 08 Oct 2020 20:35:52: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:35:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:35:52: #1 tags after filtering in treatment: 33886921 INFO @ Thu, 08 Oct 2020 20:35:52: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:35:52: #1 finished! INFO @ Thu, 08 Oct 2020 20:35:52: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:35:52: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:35:52: 29000000 INFO @ Thu, 08 Oct 2020 20:35:54: #2 number of paired peaks: 33 WARNING @ Thu, 08 Oct 2020 20:35:54: Too few paired peaks (33) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:35:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7879251/SRX7879251.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879251/SRX7879251.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879251/SRX7879251.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879251/SRX7879251.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 20:35:58: 30000000 INFO @ Thu, 08 Oct 2020 20:36:04: 31000000 INFO @ Thu, 08 Oct 2020 20:36:09: 32000000 BigWig に変換しました。 INFO @ Thu, 08 Oct 2020 20:36:15: 33000000 INFO @ Thu, 08 Oct 2020 20:36:20: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:36:20: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:36:20: #1 total tags in treatment: 33886921 INFO @ Thu, 08 Oct 2020 20:36:20: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:36:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:36:20: #1 tags after filtering in treatment: 33886921 INFO @ Thu, 08 Oct 2020 20:36:20: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:36:20: #1 finished! INFO @ Thu, 08 Oct 2020 20:36:20: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:36:20: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:36:22: #2 number of paired peaks: 33 WARNING @ Thu, 08 Oct 2020 20:36:22: Too few paired peaks (33) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:36:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7879251/SRX7879251.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879251/SRX7879251.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879251/SRX7879251.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879251/SRX7879251.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling