Job ID = 10165942 SRX = SRX7879248 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:24 43398663 reads; of these: 43398663 (100.00%) were unpaired; of these: 699472 (1.61%) aligned 0 times 35755317 (82.39%) aligned exactly 1 time 6943874 (16.00%) aligned >1 times 98.39% overall alignment rate Time searching: 00:09:24 Overall time: 00:09:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 7319054 / 42699191 = 0.1714 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:32:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7879248/SRX7879248.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7879248/SRX7879248.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7879248/SRX7879248.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7879248/SRX7879248.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:32:07: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:32:07: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:32:12: 1000000 INFO @ Thu, 08 Oct 2020 20:32:17: 2000000 INFO @ Thu, 08 Oct 2020 20:32:22: 3000000 INFO @ Thu, 08 Oct 2020 20:32:27: 4000000 INFO @ Thu, 08 Oct 2020 20:32:32: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:32:37: 6000000 INFO @ Thu, 08 Oct 2020 20:32:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7879248/SRX7879248.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7879248/SRX7879248.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7879248/SRX7879248.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7879248/SRX7879248.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:32:38: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:32:38: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:32:42: 7000000 INFO @ Thu, 08 Oct 2020 20:32:44: 1000000 INFO @ Thu, 08 Oct 2020 20:32:48: 8000000 INFO @ Thu, 08 Oct 2020 20:32:49: 2000000 INFO @ Thu, 08 Oct 2020 20:32:53: 9000000 INFO @ Thu, 08 Oct 2020 20:32:54: 3000000 INFO @ Thu, 08 Oct 2020 20:32:58: 10000000 INFO @ Thu, 08 Oct 2020 20:32:59: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:33:03: 11000000 INFO @ Thu, 08 Oct 2020 20:33:05: 5000000 INFO @ Thu, 08 Oct 2020 20:33:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7879248/SRX7879248.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7879248/SRX7879248.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7879248/SRX7879248.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7879248/SRX7879248.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:33:05: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:33:05: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:33:08: 12000000 INFO @ Thu, 08 Oct 2020 20:33:10: 6000000 INFO @ Thu, 08 Oct 2020 20:33:11: 1000000 INFO @ Thu, 08 Oct 2020 20:33:13: 13000000 INFO @ Thu, 08 Oct 2020 20:33:15: 7000000 INFO @ Thu, 08 Oct 2020 20:33:16: 2000000 INFO @ Thu, 08 Oct 2020 20:33:19: 14000000 INFO @ Thu, 08 Oct 2020 20:33:21: 8000000 INFO @ Thu, 08 Oct 2020 20:33:21: 3000000 INFO @ Thu, 08 Oct 2020 20:33:24: 15000000 INFO @ Thu, 08 Oct 2020 20:33:26: 9000000 INFO @ Thu, 08 Oct 2020 20:33:26: 4000000 INFO @ Thu, 08 Oct 2020 20:33:29: 16000000 INFO @ Thu, 08 Oct 2020 20:33:31: 10000000 INFO @ Thu, 08 Oct 2020 20:33:32: 5000000 INFO @ Thu, 08 Oct 2020 20:33:34: 17000000 INFO @ Thu, 08 Oct 2020 20:33:36: 11000000 INFO @ Thu, 08 Oct 2020 20:33:37: 6000000 INFO @ Thu, 08 Oct 2020 20:33:40: 18000000 INFO @ Thu, 08 Oct 2020 20:33:42: 12000000 INFO @ Thu, 08 Oct 2020 20:33:42: 7000000 INFO @ Thu, 08 Oct 2020 20:33:45: 19000000 INFO @ Thu, 08 Oct 2020 20:33:47: 13000000 INFO @ Thu, 08 Oct 2020 20:33:48: 8000000 INFO @ Thu, 08 Oct 2020 20:33:50: 20000000 INFO @ Thu, 08 Oct 2020 20:33:52: 14000000 INFO @ Thu, 08 Oct 2020 20:33:53: 9000000 INFO @ Thu, 08 Oct 2020 20:33:55: 21000000 INFO @ Thu, 08 Oct 2020 20:33:57: 15000000 INFO @ Thu, 08 Oct 2020 20:33:58: 10000000 INFO @ Thu, 08 Oct 2020 20:34:01: 22000000 INFO @ Thu, 08 Oct 2020 20:34:03: 16000000 INFO @ Thu, 08 Oct 2020 20:34:03: 11000000 INFO @ Thu, 08 Oct 2020 20:34:06: 23000000 INFO @ Thu, 08 Oct 2020 20:34:08: 17000000 INFO @ Thu, 08 Oct 2020 20:34:08: 12000000 INFO @ Thu, 08 Oct 2020 20:34:11: 24000000 INFO @ Thu, 08 Oct 2020 20:34:13: 18000000 INFO @ Thu, 08 Oct 2020 20:34:14: 13000000 INFO @ Thu, 08 Oct 2020 20:34:16: 25000000 INFO @ Thu, 08 Oct 2020 20:34:18: 19000000 INFO @ Thu, 08 Oct 2020 20:34:19: 14000000 INFO @ Thu, 08 Oct 2020 20:34:22: 26000000 INFO @ Thu, 08 Oct 2020 20:34:24: 20000000 INFO @ Thu, 08 Oct 2020 20:34:24: 15000000 INFO @ Thu, 08 Oct 2020 20:34:27: 27000000 INFO @ Thu, 08 Oct 2020 20:34:29: 21000000 INFO @ Thu, 08 Oct 2020 20:34:29: 16000000 INFO @ Thu, 08 Oct 2020 20:34:32: 28000000 INFO @ Thu, 08 Oct 2020 20:34:34: 22000000 INFO @ Thu, 08 Oct 2020 20:34:35: 17000000 INFO @ Thu, 08 Oct 2020 20:34:37: 29000000 INFO @ Thu, 08 Oct 2020 20:34:40: 23000000 INFO @ Thu, 08 Oct 2020 20:34:40: 18000000 INFO @ Thu, 08 Oct 2020 20:34:42: 30000000 INFO @ Thu, 08 Oct 2020 20:34:45: 24000000 INFO @ Thu, 08 Oct 2020 20:34:45: 19000000 INFO @ Thu, 08 Oct 2020 20:34:48: 31000000 INFO @ Thu, 08 Oct 2020 20:34:50: 25000000 INFO @ Thu, 08 Oct 2020 20:34:51: 20000000 INFO @ Thu, 08 Oct 2020 20:34:53: 32000000 INFO @ Thu, 08 Oct 2020 20:34:56: 26000000 INFO @ Thu, 08 Oct 2020 20:34:56: 21000000 INFO @ Thu, 08 Oct 2020 20:34:58: 33000000 INFO @ Thu, 08 Oct 2020 20:35:01: 27000000 INFO @ Thu, 08 Oct 2020 20:35:01: 22000000 INFO @ Thu, 08 Oct 2020 20:35:03: 34000000 INFO @ Thu, 08 Oct 2020 20:35:06: 28000000 INFO @ Thu, 08 Oct 2020 20:35:07: 23000000 INFO @ Thu, 08 Oct 2020 20:35:09: 35000000 INFO @ Thu, 08 Oct 2020 20:35:11: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:35:11: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:35:11: #1 total tags in treatment: 35380137 INFO @ Thu, 08 Oct 2020 20:35:11: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:35:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:35:11: #1 tags after filtering in treatment: 35380137 INFO @ Thu, 08 Oct 2020 20:35:11: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:35:11: #1 finished! INFO @ Thu, 08 Oct 2020 20:35:11: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:35:11: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:35:12: 29000000 INFO @ Thu, 08 Oct 2020 20:35:12: 24000000 INFO @ Thu, 08 Oct 2020 20:35:13: #2 number of paired peaks: 37 WARNING @ Thu, 08 Oct 2020 20:35:13: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:35:13: Process for pairing-model is terminated! INFO @ Thu, 08 Oct 2020 20:35:17: 30000000 INFO @ Thu, 08 Oct 2020 20:35:17: 25000000 INFO @ Thu, 08 Oct 2020 20:35:22: 31000000 INFO @ Thu, 08 Oct 2020 20:35:22: 26000000 cut: /home/okishinya/chipatlas/results/ce11/SRX7879248/SRX7879248.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879248/SRX7879248.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879248/SRX7879248.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879248/SRX7879248.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 20:35:27: 32000000 INFO @ Thu, 08 Oct 2020 20:35:28: 27000000 INFO @ Thu, 08 Oct 2020 20:35:33: 33000000 INFO @ Thu, 08 Oct 2020 20:35:33: 28000000 INFO @ Thu, 08 Oct 2020 20:35:38: 34000000 INFO @ Thu, 08 Oct 2020 20:35:38: 29000000 INFO @ Thu, 08 Oct 2020 20:35:43: 35000000 INFO @ Thu, 08 Oct 2020 20:35:43: 30000000 INFO @ Thu, 08 Oct 2020 20:35:45: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:35:45: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:35:45: #1 total tags in treatment: 35380137 INFO @ Thu, 08 Oct 2020 20:35:45: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:35:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:35:46: #1 tags after filtering in treatment: 35380137 INFO @ Thu, 08 Oct 2020 20:35:46: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:35:46: #1 finished! INFO @ Thu, 08 Oct 2020 20:35:46: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:35:46: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:35:48: #2 number of paired peaks: 37 WARNING @ Thu, 08 Oct 2020 20:35:48: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:35:48: Process for pairing-model is terminated! INFO @ Thu, 08 Oct 2020 20:35:48: 31000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 08 Oct 2020 20:35:53: 32000000 INFO @ Thu, 08 Oct 2020 20:35:58: 33000000 cut: /home/okishinya/chipatlas/results/ce11/SRX7879248/SRX7879248.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879248/SRX7879248.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879248/SRX7879248.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879248/SRX7879248.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 20:36:03: 34000000 INFO @ Thu, 08 Oct 2020 20:36:08: 35000000 INFO @ Thu, 08 Oct 2020 20:36:10: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:36:10: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:36:10: #1 total tags in treatment: 35380137 INFO @ Thu, 08 Oct 2020 20:36:10: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:36:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:36:10: #1 tags after filtering in treatment: 35380137 INFO @ Thu, 08 Oct 2020 20:36:10: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:36:10: #1 finished! INFO @ Thu, 08 Oct 2020 20:36:10: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:36:10: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:36:12: #2 number of paired peaks: 37 WARNING @ Thu, 08 Oct 2020 20:36:12: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:36:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX7879248/SRX7879248.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879248/SRX7879248.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879248/SRX7879248.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879248/SRX7879248.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。