Job ID = 10165945 SRX = SRX7879247 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:20 46495373 reads; of these: 46495373 (100.00%) were unpaired; of these: 7328192 (15.76%) aligned 0 times 32701101 (70.33%) aligned exactly 1 time 6466080 (13.91%) aligned >1 times 84.24% overall alignment rate Time searching: 00:09:20 Overall time: 00:09:20 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 6571521 / 39167181 = 0.1678 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:31:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7879247/SRX7879247.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7879247/SRX7879247.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7879247/SRX7879247.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7879247/SRX7879247.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:31:47: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:31:47: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:31:52: 1000000 INFO @ Thu, 08 Oct 2020 20:31:58: 2000000 INFO @ Thu, 08 Oct 2020 20:32:03: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:32:09: 4000000 INFO @ Thu, 08 Oct 2020 20:32:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7879247/SRX7879247.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7879247/SRX7879247.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7879247/SRX7879247.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7879247/SRX7879247.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:32:13: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:32:13: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:32:15: 5000000 INFO @ Thu, 08 Oct 2020 20:32:19: 1000000 INFO @ Thu, 08 Oct 2020 20:32:21: 6000000 INFO @ Thu, 08 Oct 2020 20:32:25: 2000000 INFO @ Thu, 08 Oct 2020 20:32:28: 7000000 INFO @ Thu, 08 Oct 2020 20:32:32: 3000000 INFO @ Thu, 08 Oct 2020 20:32:34: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:32:38: 4000000 INFO @ Thu, 08 Oct 2020 20:32:40: 9000000 INFO @ Thu, 08 Oct 2020 20:32:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX7879247/SRX7879247.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX7879247/SRX7879247.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX7879247/SRX7879247.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX7879247/SRX7879247.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:32:44: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:32:44: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:32:44: 5000000 INFO @ Thu, 08 Oct 2020 20:32:47: 10000000 INFO @ Thu, 08 Oct 2020 20:32:50: 1000000 INFO @ Thu, 08 Oct 2020 20:32:50: 6000000 INFO @ Thu, 08 Oct 2020 20:32:53: 11000000 INFO @ Thu, 08 Oct 2020 20:32:57: 2000000 INFO @ Thu, 08 Oct 2020 20:32:57: 7000000 INFO @ Thu, 08 Oct 2020 20:32:59: 12000000 INFO @ Thu, 08 Oct 2020 20:33:03: 3000000 INFO @ Thu, 08 Oct 2020 20:33:03: 8000000 INFO @ Thu, 08 Oct 2020 20:33:06: 13000000 INFO @ Thu, 08 Oct 2020 20:33:09: 4000000 INFO @ Thu, 08 Oct 2020 20:33:10: 9000000 INFO @ Thu, 08 Oct 2020 20:33:12: 14000000 INFO @ Thu, 08 Oct 2020 20:33:16: 5000000 INFO @ Thu, 08 Oct 2020 20:33:16: 10000000 INFO @ Thu, 08 Oct 2020 20:33:18: 15000000 INFO @ Thu, 08 Oct 2020 20:33:22: 6000000 INFO @ Thu, 08 Oct 2020 20:33:23: 11000000 INFO @ Thu, 08 Oct 2020 20:33:25: 16000000 INFO @ Thu, 08 Oct 2020 20:33:29: 7000000 INFO @ Thu, 08 Oct 2020 20:33:29: 12000000 INFO @ Thu, 08 Oct 2020 20:33:31: 17000000 INFO @ Thu, 08 Oct 2020 20:33:35: 8000000 INFO @ Thu, 08 Oct 2020 20:33:35: 13000000 INFO @ Thu, 08 Oct 2020 20:33:38: 18000000 INFO @ Thu, 08 Oct 2020 20:33:42: 9000000 INFO @ Thu, 08 Oct 2020 20:33:42: 14000000 INFO @ Thu, 08 Oct 2020 20:33:44: 19000000 INFO @ Thu, 08 Oct 2020 20:33:48: 10000000 INFO @ Thu, 08 Oct 2020 20:33:48: 15000000 INFO @ Thu, 08 Oct 2020 20:33:51: 20000000 INFO @ Thu, 08 Oct 2020 20:33:54: 11000000 INFO @ Thu, 08 Oct 2020 20:33:55: 16000000 INFO @ Thu, 08 Oct 2020 20:33:57: 21000000 INFO @ Thu, 08 Oct 2020 20:34:01: 12000000 INFO @ Thu, 08 Oct 2020 20:34:01: 17000000 INFO @ Thu, 08 Oct 2020 20:34:04: 22000000 INFO @ Thu, 08 Oct 2020 20:34:07: 13000000 INFO @ Thu, 08 Oct 2020 20:34:07: 18000000 INFO @ Thu, 08 Oct 2020 20:34:10: 23000000 INFO @ Thu, 08 Oct 2020 20:34:14: 14000000 INFO @ Thu, 08 Oct 2020 20:34:14: 19000000 INFO @ Thu, 08 Oct 2020 20:34:17: 24000000 INFO @ Thu, 08 Oct 2020 20:34:20: 20000000 INFO @ Thu, 08 Oct 2020 20:34:20: 15000000 INFO @ Thu, 08 Oct 2020 20:34:23: 25000000 INFO @ Thu, 08 Oct 2020 20:34:26: 21000000 INFO @ Thu, 08 Oct 2020 20:34:27: 16000000 INFO @ Thu, 08 Oct 2020 20:34:30: 26000000 INFO @ Thu, 08 Oct 2020 20:34:33: 22000000 INFO @ Thu, 08 Oct 2020 20:34:33: 17000000 INFO @ Thu, 08 Oct 2020 20:34:36: 27000000 INFO @ Thu, 08 Oct 2020 20:34:39: 23000000 INFO @ Thu, 08 Oct 2020 20:34:40: 18000000 INFO @ Thu, 08 Oct 2020 20:34:42: 28000000 INFO @ Thu, 08 Oct 2020 20:34:45: 24000000 INFO @ Thu, 08 Oct 2020 20:34:46: 19000000 INFO @ Thu, 08 Oct 2020 20:34:49: 29000000 INFO @ Thu, 08 Oct 2020 20:34:52: 25000000 INFO @ Thu, 08 Oct 2020 20:34:52: 20000000 INFO @ Thu, 08 Oct 2020 20:34:55: 30000000 INFO @ Thu, 08 Oct 2020 20:34:58: 26000000 INFO @ Thu, 08 Oct 2020 20:34:59: 21000000 INFO @ Thu, 08 Oct 2020 20:35:01: 31000000 INFO @ Thu, 08 Oct 2020 20:35:04: 27000000 INFO @ Thu, 08 Oct 2020 20:35:05: 22000000 INFO @ Thu, 08 Oct 2020 20:35:08: 32000000 INFO @ Thu, 08 Oct 2020 20:35:10: 28000000 INFO @ Thu, 08 Oct 2020 20:35:11: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:35:11: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:35:11: #1 total tags in treatment: 32595660 INFO @ Thu, 08 Oct 2020 20:35:11: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:35:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:35:11: 23000000 INFO @ Thu, 08 Oct 2020 20:35:12: #1 tags after filtering in treatment: 32595660 INFO @ Thu, 08 Oct 2020 20:35:12: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:35:12: #1 finished! INFO @ Thu, 08 Oct 2020 20:35:12: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:35:12: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 08 Oct 2020 20:35:14: #2 number of paired peaks: 70 WARNING @ Thu, 08 Oct 2020 20:35:14: Too few paired peaks (70) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:35:14: Process for pairing-model is terminated! INFO @ Thu, 08 Oct 2020 20:35:17: 29000000 INFO @ Thu, 08 Oct 2020 20:35:18: 24000000 cut: /home/okishinya/chipatlas/results/ce11/SRX7879247/SRX7879247.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879247/SRX7879247.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879247/SRX7879247.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879247/SRX7879247.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 20:35:23: 30000000 INFO @ Thu, 08 Oct 2020 20:35:24: 25000000 INFO @ Thu, 08 Oct 2020 20:35:29: 31000000 INFO @ Thu, 08 Oct 2020 20:35:30: 26000000 INFO @ Thu, 08 Oct 2020 20:35:35: 32000000 INFO @ Thu, 08 Oct 2020 20:35:37: 27000000 INFO @ Thu, 08 Oct 2020 20:35:39: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:35:39: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:35:39: #1 total tags in treatment: 32595660 INFO @ Thu, 08 Oct 2020 20:35:39: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:35:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:35:40: #1 tags after filtering in treatment: 32595660 INFO @ Thu, 08 Oct 2020 20:35:40: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:35:40: #1 finished! INFO @ Thu, 08 Oct 2020 20:35:40: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:35:40: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:35:42: #2 number of paired peaks: 70 WARNING @ Thu, 08 Oct 2020 20:35:42: Too few paired peaks (70) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:35:42: Process for pairing-model is terminated! INFO @ Thu, 08 Oct 2020 20:35:43: 28000000 INFO @ Thu, 08 Oct 2020 20:35:49: 29000000 cut: /home/okishinya/chipatlas/results/ce11/SRX7879247/SRX7879247.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879247/SRX7879247.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879247/SRX7879247.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879247/SRX7879247.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 20:35:54: 30000000 INFO @ Thu, 08 Oct 2020 20:36:00: 31000000 INFO @ Thu, 08 Oct 2020 20:36:06: 32000000 INFO @ Thu, 08 Oct 2020 20:36:10: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:36:10: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:36:10: #1 total tags in treatment: 32595660 INFO @ Thu, 08 Oct 2020 20:36:10: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:36:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:36:10: #1 tags after filtering in treatment: 32595660 INFO @ Thu, 08 Oct 2020 20:36:10: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:36:10: #1 finished! INFO @ Thu, 08 Oct 2020 20:36:10: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:36:10: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:36:12: #2 number of paired peaks: 70 WARNING @ Thu, 08 Oct 2020 20:36:12: Too few paired peaks (70) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:36:12: Process for pairing-model is terminated! BigWig に変換しました。 cut: /home/okishinya/chipatlas/results/ce11/SRX7879247/SRX7879247.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879247/SRX7879247.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879247/SRX7879247.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX7879247/SRX7879247.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling