Job ID = 6368915 SRX = SRX707299 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-16T00:32:27 prefetch.2.10.7: 1) Downloading 'SRR1582082'... 2020-06-16T00:32:27 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:37:26 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:37:26 prefetch.2.10.7: 1) 'SRR1582082' was downloaded successfully Read 54428125 spots for SRR1582082/SRR1582082.sra Written 54428125 spots for SRR1582082/SRR1582082.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:23 54428125 reads; of these: 54428125 (100.00%) were unpaired; of these: 1050429 (1.93%) aligned 0 times 44432639 (81.64%) aligned exactly 1 time 8945057 (16.43%) aligned >1 times 98.07% overall alignment rate Time searching: 00:13:23 Overall time: 00:13:23 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 13643647 / 53377696 = 0.2556 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:07:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX707299/SRX707299.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX707299/SRX707299.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX707299/SRX707299.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX707299/SRX707299.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:07:39: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:07:39: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:07:46: 1000000 INFO @ Tue, 16 Jun 2020 10:07:54: 2000000 INFO @ Tue, 16 Jun 2020 10:08:01: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:08:08: 4000000 INFO @ Tue, 16 Jun 2020 10:08:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX707299/SRX707299.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX707299/SRX707299.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX707299/SRX707299.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX707299/SRX707299.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:08:09: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:08:09: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:08:16: 5000000 INFO @ Tue, 16 Jun 2020 10:08:17: 1000000 INFO @ Tue, 16 Jun 2020 10:08:24: 6000000 INFO @ Tue, 16 Jun 2020 10:08:25: 2000000 INFO @ Tue, 16 Jun 2020 10:08:32: 7000000 INFO @ Tue, 16 Jun 2020 10:08:33: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:08:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX707299/SRX707299.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX707299/SRX707299.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX707299/SRX707299.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX707299/SRX707299.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:08:39: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:08:39: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:08:40: 8000000 INFO @ Tue, 16 Jun 2020 10:08:41: 4000000 INFO @ Tue, 16 Jun 2020 10:08:47: 1000000 INFO @ Tue, 16 Jun 2020 10:08:48: 9000000 INFO @ Tue, 16 Jun 2020 10:08:49: 5000000 INFO @ Tue, 16 Jun 2020 10:08:55: 2000000 INFO @ Tue, 16 Jun 2020 10:08:56: 10000000 INFO @ Tue, 16 Jun 2020 10:08:57: 6000000 INFO @ Tue, 16 Jun 2020 10:09:03: 3000000 INFO @ Tue, 16 Jun 2020 10:09:04: 11000000 INFO @ Tue, 16 Jun 2020 10:09:06: 7000000 INFO @ Tue, 16 Jun 2020 10:09:12: 4000000 INFO @ Tue, 16 Jun 2020 10:09:12: 12000000 INFO @ Tue, 16 Jun 2020 10:09:14: 8000000 INFO @ Tue, 16 Jun 2020 10:09:20: 13000000 INFO @ Tue, 16 Jun 2020 10:09:20: 5000000 INFO @ Tue, 16 Jun 2020 10:09:22: 9000000 INFO @ Tue, 16 Jun 2020 10:09:28: 14000000 INFO @ Tue, 16 Jun 2020 10:09:28: 6000000 INFO @ Tue, 16 Jun 2020 10:09:30: 10000000 INFO @ Tue, 16 Jun 2020 10:09:37: 15000000 INFO @ Tue, 16 Jun 2020 10:09:37: 7000000 INFO @ Tue, 16 Jun 2020 10:09:39: 11000000 INFO @ Tue, 16 Jun 2020 10:09:45: 16000000 INFO @ Tue, 16 Jun 2020 10:09:45: 8000000 INFO @ Tue, 16 Jun 2020 10:09:47: 12000000 INFO @ Tue, 16 Jun 2020 10:09:53: 17000000 INFO @ Tue, 16 Jun 2020 10:09:54: 9000000 INFO @ Tue, 16 Jun 2020 10:09:55: 13000000 INFO @ Tue, 16 Jun 2020 10:10:02: 18000000 INFO @ Tue, 16 Jun 2020 10:10:02: 10000000 INFO @ Tue, 16 Jun 2020 10:10:04: 14000000 INFO @ Tue, 16 Jun 2020 10:10:10: 19000000 INFO @ Tue, 16 Jun 2020 10:10:11: 11000000 INFO @ Tue, 16 Jun 2020 10:10:12: 15000000 INFO @ Tue, 16 Jun 2020 10:10:19: 20000000 INFO @ Tue, 16 Jun 2020 10:10:20: 12000000 INFO @ Tue, 16 Jun 2020 10:10:21: 16000000 INFO @ Tue, 16 Jun 2020 10:10:27: 21000000 INFO @ Tue, 16 Jun 2020 10:10:28: 13000000 INFO @ Tue, 16 Jun 2020 10:10:29: 17000000 INFO @ Tue, 16 Jun 2020 10:10:36: 22000000 INFO @ Tue, 16 Jun 2020 10:10:37: 14000000 INFO @ Tue, 16 Jun 2020 10:10:38: 18000000 INFO @ Tue, 16 Jun 2020 10:10:44: 23000000 INFO @ Tue, 16 Jun 2020 10:10:46: 15000000 INFO @ Tue, 16 Jun 2020 10:10:47: 19000000 INFO @ Tue, 16 Jun 2020 10:10:53: 24000000 INFO @ Tue, 16 Jun 2020 10:10:54: 16000000 INFO @ Tue, 16 Jun 2020 10:10:55: 20000000 INFO @ Tue, 16 Jun 2020 10:11:01: 25000000 INFO @ Tue, 16 Jun 2020 10:11:03: 17000000 INFO @ Tue, 16 Jun 2020 10:11:04: 21000000 INFO @ Tue, 16 Jun 2020 10:11:09: 26000000 INFO @ Tue, 16 Jun 2020 10:11:11: 18000000 INFO @ Tue, 16 Jun 2020 10:11:12: 22000000 INFO @ Tue, 16 Jun 2020 10:11:18: 27000000 INFO @ Tue, 16 Jun 2020 10:11:20: 19000000 INFO @ Tue, 16 Jun 2020 10:11:21: 23000000 INFO @ Tue, 16 Jun 2020 10:11:27: 28000000 INFO @ Tue, 16 Jun 2020 10:11:29: 20000000 INFO @ Tue, 16 Jun 2020 10:11:29: 24000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 10:11:35: 29000000 INFO @ Tue, 16 Jun 2020 10:11:38: 25000000 INFO @ Tue, 16 Jun 2020 10:11:38: 21000000 INFO @ Tue, 16 Jun 2020 10:11:44: 30000000 INFO @ Tue, 16 Jun 2020 10:11:46: 26000000 INFO @ Tue, 16 Jun 2020 10:11:47: 22000000 INFO @ Tue, 16 Jun 2020 10:11:53: 31000000 INFO @ Tue, 16 Jun 2020 10:11:55: 27000000 INFO @ Tue, 16 Jun 2020 10:11:55: 23000000 INFO @ Tue, 16 Jun 2020 10:12:01: 32000000 INFO @ Tue, 16 Jun 2020 10:12:03: 28000000 INFO @ Tue, 16 Jun 2020 10:12:04: 24000000 INFO @ Tue, 16 Jun 2020 10:12:10: 33000000 INFO @ Tue, 16 Jun 2020 10:12:12: 29000000 INFO @ Tue, 16 Jun 2020 10:12:12: 25000000 INFO @ Tue, 16 Jun 2020 10:12:19: 34000000 INFO @ Tue, 16 Jun 2020 10:12:21: 30000000 INFO @ Tue, 16 Jun 2020 10:12:21: 26000000 INFO @ Tue, 16 Jun 2020 10:12:28: 35000000 INFO @ Tue, 16 Jun 2020 10:12:29: 31000000 INFO @ Tue, 16 Jun 2020 10:12:29: 27000000 INFO @ Tue, 16 Jun 2020 10:12:37: 36000000 INFO @ Tue, 16 Jun 2020 10:12:38: 32000000 INFO @ Tue, 16 Jun 2020 10:12:38: 28000000 INFO @ Tue, 16 Jun 2020 10:12:46: 37000000 INFO @ Tue, 16 Jun 2020 10:12:46: 33000000 INFO @ Tue, 16 Jun 2020 10:12:47: 29000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 10:12:54: 38000000 INFO @ Tue, 16 Jun 2020 10:12:55: 34000000 INFO @ Tue, 16 Jun 2020 10:12:55: 30000000 INFO @ Tue, 16 Jun 2020 10:13:03: 39000000 INFO @ Tue, 16 Jun 2020 10:13:03: 35000000 INFO @ Tue, 16 Jun 2020 10:13:04: 31000000 INFO @ Tue, 16 Jun 2020 10:13:09: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 10:13:09: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 10:13:09: #1 total tags in treatment: 39734049 INFO @ Tue, 16 Jun 2020 10:13:09: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:13:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:13:10: #1 tags after filtering in treatment: 39734049 INFO @ Tue, 16 Jun 2020 10:13:10: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:13:10: #1 finished! INFO @ Tue, 16 Jun 2020 10:13:10: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:13:10: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:13:12: 36000000 INFO @ Tue, 16 Jun 2020 10:13:12: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 10:13:12: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:13:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX707299/SRX707299.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX707299/SRX707299.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX707299/SRX707299.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX707299/SRX707299.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:13:13: 32000000 INFO @ Tue, 16 Jun 2020 10:13:20: 37000000 INFO @ Tue, 16 Jun 2020 10:13:21: 33000000 INFO @ Tue, 16 Jun 2020 10:13:28: 38000000 INFO @ Tue, 16 Jun 2020 10:13:30: 34000000 INFO @ Tue, 16 Jun 2020 10:13:37: 39000000 INFO @ Tue, 16 Jun 2020 10:13:39: 35000000 INFO @ Tue, 16 Jun 2020 10:13:43: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 10:13:43: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 10:13:43: #1 total tags in treatment: 39734049 INFO @ Tue, 16 Jun 2020 10:13:43: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:13:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:13:44: #1 tags after filtering in treatment: 39734049 INFO @ Tue, 16 Jun 2020 10:13:44: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:13:44: #1 finished! INFO @ Tue, 16 Jun 2020 10:13:44: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:13:44: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:13:46: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 10:13:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:13:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX707299/SRX707299.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX707299/SRX707299.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX707299/SRX707299.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX707299/SRX707299.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:13:47: 36000000 INFO @ Tue, 16 Jun 2020 10:13:55: 37000000 INFO @ Tue, 16 Jun 2020 10:14:03: 38000000 INFO @ Tue, 16 Jun 2020 10:14:12: 39000000 INFO @ Tue, 16 Jun 2020 10:14:18: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 10:14:18: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 10:14:18: #1 total tags in treatment: 39734049 INFO @ Tue, 16 Jun 2020 10:14:18: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:14:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:14:18: #1 tags after filtering in treatment: 39734049 INFO @ Tue, 16 Jun 2020 10:14:18: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:14:18: #1 finished! INFO @ Tue, 16 Jun 2020 10:14:18: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:14:18: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:14:21: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 10:14:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:14:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX707299/SRX707299.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX707299/SRX707299.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX707299/SRX707299.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX707299/SRX707299.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling