Job ID = 6368892 SRX = SRX6619594 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-16T00:37:35 prefetch.2.10.7: 1) Downloading 'SRR9866014'... 2020-06-16T00:37:35 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:43:02 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:43:02 prefetch.2.10.7: 1) 'SRR9866014' was downloaded successfully 2020-06-16T00:43:02 prefetch.2.10.7: 'SRR9866014' has 0 unresolved dependencies Read 60566309 spots for SRR9866014/SRR9866014.sra Written 60566309 spots for SRR9866014/SRR9866014.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:26 60566309 reads; of these: 60566309 (100.00%) were unpaired; of these: 2547423 (4.21%) aligned 0 times 49309705 (81.41%) aligned exactly 1 time 8709181 (14.38%) aligned >1 times 95.79% overall alignment rate Time searching: 00:13:26 Overall time: 00:13:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 15511167 / 58018886 = 0.2673 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:12:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX6619594/SRX6619594.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX6619594/SRX6619594.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX6619594/SRX6619594.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX6619594/SRX6619594.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:12:25: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:12:25: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:12:31: 1000000 INFO @ Tue, 16 Jun 2020 10:12:37: 2000000 INFO @ Tue, 16 Jun 2020 10:12:43: 3000000 INFO @ Tue, 16 Jun 2020 10:12:50: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:12:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX6619594/SRX6619594.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX6619594/SRX6619594.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX6619594/SRX6619594.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX6619594/SRX6619594.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:12:55: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:12:55: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:12:56: 5000000 INFO @ Tue, 16 Jun 2020 10:13:02: 1000000 INFO @ Tue, 16 Jun 2020 10:13:03: 6000000 INFO @ Tue, 16 Jun 2020 10:13:10: 2000000 INFO @ Tue, 16 Jun 2020 10:13:10: 7000000 INFO @ Tue, 16 Jun 2020 10:13:17: 8000000 INFO @ Tue, 16 Jun 2020 10:13:18: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:13:24: 9000000 INFO @ Tue, 16 Jun 2020 10:13:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX6619594/SRX6619594.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX6619594/SRX6619594.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX6619594/SRX6619594.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX6619594/SRX6619594.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:13:25: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:13:25: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:13:25: 4000000 INFO @ Tue, 16 Jun 2020 10:13:32: 10000000 INFO @ Tue, 16 Jun 2020 10:13:32: 1000000 INFO @ Tue, 16 Jun 2020 10:13:33: 5000000 INFO @ Tue, 16 Jun 2020 10:13:39: 11000000 INFO @ Tue, 16 Jun 2020 10:13:40: 2000000 INFO @ Tue, 16 Jun 2020 10:13:41: 6000000 INFO @ Tue, 16 Jun 2020 10:13:47: 12000000 INFO @ Tue, 16 Jun 2020 10:13:47: 3000000 INFO @ Tue, 16 Jun 2020 10:13:48: 7000000 INFO @ Tue, 16 Jun 2020 10:13:54: 13000000 INFO @ Tue, 16 Jun 2020 10:13:54: 4000000 INFO @ Tue, 16 Jun 2020 10:13:56: 8000000 INFO @ Tue, 16 Jun 2020 10:14:01: 14000000 INFO @ Tue, 16 Jun 2020 10:14:02: 5000000 INFO @ Tue, 16 Jun 2020 10:14:04: 9000000 INFO @ Tue, 16 Jun 2020 10:14:09: 15000000 INFO @ Tue, 16 Jun 2020 10:14:09: 6000000 INFO @ Tue, 16 Jun 2020 10:14:11: 10000000 INFO @ Tue, 16 Jun 2020 10:14:16: 16000000 INFO @ Tue, 16 Jun 2020 10:14:16: 7000000 INFO @ Tue, 16 Jun 2020 10:14:19: 11000000 INFO @ Tue, 16 Jun 2020 10:14:23: 17000000 INFO @ Tue, 16 Jun 2020 10:14:24: 8000000 INFO @ Tue, 16 Jun 2020 10:14:27: 12000000 INFO @ Tue, 16 Jun 2020 10:14:31: 18000000 INFO @ Tue, 16 Jun 2020 10:14:31: 9000000 INFO @ Tue, 16 Jun 2020 10:14:34: 13000000 INFO @ Tue, 16 Jun 2020 10:14:38: 19000000 INFO @ Tue, 16 Jun 2020 10:14:39: 10000000 INFO @ Tue, 16 Jun 2020 10:14:42: 14000000 INFO @ Tue, 16 Jun 2020 10:14:46: 20000000 INFO @ Tue, 16 Jun 2020 10:14:46: 11000000 INFO @ Tue, 16 Jun 2020 10:14:50: 15000000 INFO @ Tue, 16 Jun 2020 10:14:53: 21000000 INFO @ Tue, 16 Jun 2020 10:14:53: 12000000 INFO @ Tue, 16 Jun 2020 10:14:57: 16000000 INFO @ Tue, 16 Jun 2020 10:15:01: 22000000 INFO @ Tue, 16 Jun 2020 10:15:01: 13000000 INFO @ Tue, 16 Jun 2020 10:15:05: 17000000 INFO @ Tue, 16 Jun 2020 10:15:08: 23000000 INFO @ Tue, 16 Jun 2020 10:15:08: 14000000 INFO @ Tue, 16 Jun 2020 10:15:12: 18000000 INFO @ Tue, 16 Jun 2020 10:15:16: 24000000 INFO @ Tue, 16 Jun 2020 10:15:16: 15000000 INFO @ Tue, 16 Jun 2020 10:15:20: 19000000 INFO @ Tue, 16 Jun 2020 10:15:23: 25000000 INFO @ Tue, 16 Jun 2020 10:15:24: 16000000 INFO @ Tue, 16 Jun 2020 10:15:28: 20000000 INFO @ Tue, 16 Jun 2020 10:15:31: 26000000 INFO @ Tue, 16 Jun 2020 10:15:31: 17000000 INFO @ Tue, 16 Jun 2020 10:15:36: 21000000 INFO @ Tue, 16 Jun 2020 10:15:38: 27000000 INFO @ Tue, 16 Jun 2020 10:15:39: 18000000 INFO @ Tue, 16 Jun 2020 10:15:44: 22000000 INFO @ Tue, 16 Jun 2020 10:15:46: 28000000 INFO @ Tue, 16 Jun 2020 10:15:46: 19000000 INFO @ Tue, 16 Jun 2020 10:15:52: 23000000 INFO @ Tue, 16 Jun 2020 10:15:54: 29000000 INFO @ Tue, 16 Jun 2020 10:15:54: 20000000 INFO @ Tue, 16 Jun 2020 10:16:00: 24000000 INFO @ Tue, 16 Jun 2020 10:16:01: 30000000 INFO @ Tue, 16 Jun 2020 10:16:02: 21000000 INFO @ Tue, 16 Jun 2020 10:16:09: 25000000 INFO @ Tue, 16 Jun 2020 10:16:09: 31000000 INFO @ Tue, 16 Jun 2020 10:16:09: 22000000 INFO @ Tue, 16 Jun 2020 10:16:17: 32000000 INFO @ Tue, 16 Jun 2020 10:16:17: 26000000 INFO @ Tue, 16 Jun 2020 10:16:17: 23000000 INFO @ Tue, 16 Jun 2020 10:16:25: 33000000 INFO @ Tue, 16 Jun 2020 10:16:25: 24000000 INFO @ Tue, 16 Jun 2020 10:16:25: 27000000 INFO @ Tue, 16 Jun 2020 10:16:32: 34000000 INFO @ Tue, 16 Jun 2020 10:16:32: 25000000 INFO @ Tue, 16 Jun 2020 10:16:33: 28000000 INFO @ Tue, 16 Jun 2020 10:16:40: 26000000 INFO @ Tue, 16 Jun 2020 10:16:40: 35000000 INFO @ Tue, 16 Jun 2020 10:16:41: 29000000 INFO @ Tue, 16 Jun 2020 10:16:47: 27000000 INFO @ Tue, 16 Jun 2020 10:16:48: 36000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 10:16:50: 30000000 INFO @ Tue, 16 Jun 2020 10:16:55: 28000000 INFO @ Tue, 16 Jun 2020 10:16:56: 37000000 INFO @ Tue, 16 Jun 2020 10:16:58: 31000000 INFO @ Tue, 16 Jun 2020 10:17:03: 29000000 INFO @ Tue, 16 Jun 2020 10:17:03: 38000000 INFO @ Tue, 16 Jun 2020 10:17:06: 32000000 INFO @ Tue, 16 Jun 2020 10:17:10: 30000000 INFO @ Tue, 16 Jun 2020 10:17:11: 39000000 INFO @ Tue, 16 Jun 2020 10:17:15: 33000000 INFO @ Tue, 16 Jun 2020 10:17:18: 31000000 INFO @ Tue, 16 Jun 2020 10:17:19: 40000000 INFO @ Tue, 16 Jun 2020 10:17:24: 34000000 INFO @ Tue, 16 Jun 2020 10:17:25: 32000000 INFO @ Tue, 16 Jun 2020 10:17:27: 41000000 INFO @ Tue, 16 Jun 2020 10:17:33: 33000000 INFO @ Tue, 16 Jun 2020 10:17:33: 35000000 INFO @ Tue, 16 Jun 2020 10:17:35: 42000000 INFO @ Tue, 16 Jun 2020 10:17:39: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 10:17:39: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 10:17:39: #1 total tags in treatment: 42507719 INFO @ Tue, 16 Jun 2020 10:17:39: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:17:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:17:40: #1 tags after filtering in treatment: 42507719 INFO @ Tue, 16 Jun 2020 10:17:40: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:17:40: #1 finished! INFO @ Tue, 16 Jun 2020 10:17:40: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:17:40: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:17:40: 34000000 INFO @ Tue, 16 Jun 2020 10:17:42: 36000000 INFO @ Tue, 16 Jun 2020 10:17:43: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 10:17:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:17:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX6619594/SRX6619594.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX6619594/SRX6619594.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX6619594/SRX6619594.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX6619594/SRX6619594.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:17:48: 35000000 INFO @ Tue, 16 Jun 2020 10:17:52: 37000000 INFO @ Tue, 16 Jun 2020 10:17:55: 36000000 INFO @ Tue, 16 Jun 2020 10:18:00: 38000000 INFO @ Tue, 16 Jun 2020 10:18:02: 37000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 10:18:09: 39000000 INFO @ Tue, 16 Jun 2020 10:18:10: 38000000 INFO @ Tue, 16 Jun 2020 10:18:17: 39000000 INFO @ Tue, 16 Jun 2020 10:18:18: 40000000 INFO @ Tue, 16 Jun 2020 10:18:25: 40000000 INFO @ Tue, 16 Jun 2020 10:18:27: 41000000 INFO @ Tue, 16 Jun 2020 10:18:32: 41000000 INFO @ Tue, 16 Jun 2020 10:18:36: 42000000 INFO @ Tue, 16 Jun 2020 10:18:40: 42000000 INFO @ Tue, 16 Jun 2020 10:18:41: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 10:18:41: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 10:18:41: #1 total tags in treatment: 42507719 INFO @ Tue, 16 Jun 2020 10:18:41: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:18:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:18:42: #1 tags after filtering in treatment: 42507719 INFO @ Tue, 16 Jun 2020 10:18:42: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:18:42: #1 finished! INFO @ Tue, 16 Jun 2020 10:18:42: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:18:42: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:18:43: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 10:18:43: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 10:18:43: #1 total tags in treatment: 42507719 INFO @ Tue, 16 Jun 2020 10:18:43: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:18:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:18:44: #1 tags after filtering in treatment: 42507719 INFO @ Tue, 16 Jun 2020 10:18:44: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:18:44: #1 finished! INFO @ Tue, 16 Jun 2020 10:18:44: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:18:44: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:18:45: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 10:18:45: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:18:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX6619594/SRX6619594.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX6619594/SRX6619594.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX6619594/SRX6619594.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX6619594/SRX6619594.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:18:47: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 10:18:47: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:18:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX6619594/SRX6619594.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX6619594/SRX6619594.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX6619594/SRX6619594.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX6619594/SRX6619594.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling