Job ID = 6368846 SRX = SRX6619545 Genome = ce11 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-06-16T00:33:09 prefetch.2.10.7: 1) Downloading 'SRR9866064'... 2020-06-16T00:33:09 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:45:47 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:45:47 prefetch.2.10.7: 1) 'SRR9866064' was downloaded successfully 2020-06-16T00:45:47 prefetch.2.10.7: 'SRR9866064' has 0 unresolved dependencies Read 37516093 spots for SRR9866064/SRR9866064.sra Written 37516093 spots for SRR9866064/SRR9866064.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:21:37 37516093 reads; of these: 37516093 (100.00%) were paired; of these: 12024060 (32.05%) aligned concordantly 0 times 21531974 (57.39%) aligned concordantly exactly 1 time 3960059 (10.56%) aligned concordantly >1 times ---- 12024060 pairs aligned concordantly 0 times; of these: 8019450 (66.70%) aligned discordantly 1 time ---- 4004610 pairs aligned 0 times concordantly or discordantly; of these: 8009220 mates make up the pairs; of these: 4383373 (54.73%) aligned 0 times 1422958 (17.77%) aligned exactly 1 time 2202889 (27.50%) aligned >1 times 94.16% overall alignment rate Time searching: 01:21:37 Overall time: 01:21:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 52 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 4972846 / 33297924 = 0.1493 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 11:44:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX6619545/SRX6619545.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX6619545/SRX6619545.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX6619545/SRX6619545.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX6619545/SRX6619545.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 11:44:46: #1 read tag files... INFO @ Tue, 16 Jun 2020 11:44:46: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 11:44:54: 1000000 INFO @ Tue, 16 Jun 2020 11:45:01: 2000000 INFO @ Tue, 16 Jun 2020 11:45:08: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 11:45:15: 4000000 INFO @ Tue, 16 Jun 2020 11:45:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX6619545/SRX6619545.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX6619545/SRX6619545.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX6619545/SRX6619545.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX6619545/SRX6619545.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 11:45:16: #1 read tag files... INFO @ Tue, 16 Jun 2020 11:45:16: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 11:45:22: 5000000 INFO @ Tue, 16 Jun 2020 11:45:24: 1000000 INFO @ Tue, 16 Jun 2020 11:45:30: 6000000 INFO @ Tue, 16 Jun 2020 11:45:32: 2000000 INFO @ Tue, 16 Jun 2020 11:45:38: 7000000 INFO @ Tue, 16 Jun 2020 11:45:40: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 11:45:45: 8000000 INFO @ Tue, 16 Jun 2020 11:45:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX6619545/SRX6619545.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX6619545/SRX6619545.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX6619545/SRX6619545.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX6619545/SRX6619545.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 11:45:46: #1 read tag files... INFO @ Tue, 16 Jun 2020 11:45:46: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 11:45:47: 4000000 INFO @ Tue, 16 Jun 2020 11:45:53: 9000000 INFO @ Tue, 16 Jun 2020 11:45:54: 1000000 INFO @ Tue, 16 Jun 2020 11:45:55: 5000000 INFO @ Tue, 16 Jun 2020 11:46:01: 10000000 INFO @ Tue, 16 Jun 2020 11:46:02: 2000000 INFO @ Tue, 16 Jun 2020 11:46:03: 6000000 INFO @ Tue, 16 Jun 2020 11:46:08: 11000000 INFO @ Tue, 16 Jun 2020 11:46:09: 3000000 INFO @ Tue, 16 Jun 2020 11:46:11: 7000000 INFO @ Tue, 16 Jun 2020 11:46:16: 12000000 INFO @ Tue, 16 Jun 2020 11:46:17: 4000000 INFO @ Tue, 16 Jun 2020 11:46:18: 8000000 INFO @ Tue, 16 Jun 2020 11:46:23: 13000000 INFO @ Tue, 16 Jun 2020 11:46:24: 5000000 INFO @ Tue, 16 Jun 2020 11:46:26: 9000000 INFO @ Tue, 16 Jun 2020 11:46:31: 14000000 INFO @ Tue, 16 Jun 2020 11:46:32: 6000000 INFO @ Tue, 16 Jun 2020 11:46:34: 10000000 INFO @ Tue, 16 Jun 2020 11:46:38: 15000000 INFO @ Tue, 16 Jun 2020 11:46:39: 7000000 INFO @ Tue, 16 Jun 2020 11:46:42: 11000000 INFO @ Tue, 16 Jun 2020 11:46:46: 16000000 INFO @ Tue, 16 Jun 2020 11:46:47: 8000000 INFO @ Tue, 16 Jun 2020 11:46:49: 12000000 INFO @ Tue, 16 Jun 2020 11:46:53: 17000000 INFO @ Tue, 16 Jun 2020 11:46:54: 9000000 INFO @ Tue, 16 Jun 2020 11:46:57: 13000000 INFO @ Tue, 16 Jun 2020 11:47:01: 18000000 INFO @ Tue, 16 Jun 2020 11:47:02: 10000000 INFO @ Tue, 16 Jun 2020 11:47:05: 14000000 INFO @ Tue, 16 Jun 2020 11:47:09: 19000000 INFO @ Tue, 16 Jun 2020 11:47:09: 11000000 INFO @ Tue, 16 Jun 2020 11:47:13: 15000000 INFO @ Tue, 16 Jun 2020 11:47:17: 20000000 INFO @ Tue, 16 Jun 2020 11:47:17: 12000000 INFO @ Tue, 16 Jun 2020 11:47:20: 16000000 INFO @ Tue, 16 Jun 2020 11:47:25: 21000000 INFO @ Tue, 16 Jun 2020 11:47:25: 13000000 INFO @ Tue, 16 Jun 2020 11:47:28: 17000000 INFO @ Tue, 16 Jun 2020 11:47:32: 14000000 INFO @ Tue, 16 Jun 2020 11:47:33: 22000000 INFO @ Tue, 16 Jun 2020 11:47:36: 18000000 INFO @ Tue, 16 Jun 2020 11:47:40: 15000000 INFO @ Tue, 16 Jun 2020 11:47:41: 23000000 INFO @ Tue, 16 Jun 2020 11:47:44: 19000000 INFO @ Tue, 16 Jun 2020 11:47:48: 16000000 INFO @ Tue, 16 Jun 2020 11:47:49: 24000000 INFO @ Tue, 16 Jun 2020 11:47:52: 20000000 INFO @ Tue, 16 Jun 2020 11:47:56: 17000000 INFO @ Tue, 16 Jun 2020 11:47:57: 25000000 INFO @ Tue, 16 Jun 2020 11:48:00: 21000000 INFO @ Tue, 16 Jun 2020 11:48:04: 18000000 INFO @ Tue, 16 Jun 2020 11:48:06: 26000000 INFO @ Tue, 16 Jun 2020 11:48:08: 22000000 INFO @ Tue, 16 Jun 2020 11:48:12: 19000000 INFO @ Tue, 16 Jun 2020 11:48:14: 27000000 INFO @ Tue, 16 Jun 2020 11:48:16: 23000000 INFO @ Tue, 16 Jun 2020 11:48:19: 20000000 INFO @ Tue, 16 Jun 2020 11:48:22: 28000000 INFO @ Tue, 16 Jun 2020 11:48:24: 24000000 INFO @ Tue, 16 Jun 2020 11:48:27: 21000000 INFO @ Tue, 16 Jun 2020 11:48:30: 29000000 INFO @ Tue, 16 Jun 2020 11:48:32: 25000000 INFO @ Tue, 16 Jun 2020 11:48:35: 22000000 INFO @ Tue, 16 Jun 2020 11:48:38: 30000000 INFO @ Tue, 16 Jun 2020 11:48:40: 26000000 INFO @ Tue, 16 Jun 2020 11:48:43: 23000000 INFO @ Tue, 16 Jun 2020 11:48:47: 31000000 INFO @ Tue, 16 Jun 2020 11:48:48: 27000000 INFO @ Tue, 16 Jun 2020 11:48:51: 24000000 INFO @ Tue, 16 Jun 2020 11:48:55: 32000000 INFO @ Tue, 16 Jun 2020 11:48:57: 28000000 INFO @ Tue, 16 Jun 2020 11:48:59: 25000000 INFO @ Tue, 16 Jun 2020 11:49:03: 33000000 INFO @ Tue, 16 Jun 2020 11:49:05: 29000000 INFO @ Tue, 16 Jun 2020 11:49:07: 26000000 INFO @ Tue, 16 Jun 2020 11:49:11: 34000000 INFO @ Tue, 16 Jun 2020 11:49:13: 30000000 INFO @ Tue, 16 Jun 2020 11:49:15: 27000000 INFO @ Tue, 16 Jun 2020 11:49:19: 35000000 INFO @ Tue, 16 Jun 2020 11:49:21: 31000000 INFO @ Tue, 16 Jun 2020 11:49:23: 28000000 INFO @ Tue, 16 Jun 2020 11:49:27: 36000000 INFO @ Tue, 16 Jun 2020 11:49:29: 32000000 INFO @ Tue, 16 Jun 2020 11:49:31: 29000000 INFO @ Tue, 16 Jun 2020 11:49:35: 37000000 INFO @ Tue, 16 Jun 2020 11:49:37: 33000000 INFO @ Tue, 16 Jun 2020 11:49:39: 30000000 INFO @ Tue, 16 Jun 2020 11:49:43: 38000000 INFO @ Tue, 16 Jun 2020 11:49:45: 34000000 INFO @ Tue, 16 Jun 2020 11:49:46: 31000000 INFO @ Tue, 16 Jun 2020 11:49:51: 39000000 INFO @ Tue, 16 Jun 2020 11:49:53: 35000000 INFO @ Tue, 16 Jun 2020 11:49:54: 32000000 INFO @ Tue, 16 Jun 2020 11:49:59: 40000000 INFO @ Tue, 16 Jun 2020 11:50:00: 36000000 INFO @ Tue, 16 Jun 2020 11:50:02: 33000000 INFO @ Tue, 16 Jun 2020 11:50:06: 41000000 INFO @ Tue, 16 Jun 2020 11:50:08: 37000000 INFO @ Tue, 16 Jun 2020 11:50:10: 34000000 INFO @ Tue, 16 Jun 2020 11:50:14: 42000000 INFO @ Tue, 16 Jun 2020 11:50:16: 38000000 INFO @ Tue, 16 Jun 2020 11:50:18: 35000000 INFO @ Tue, 16 Jun 2020 11:50:21: 43000000 INFO @ Tue, 16 Jun 2020 11:50:24: 39000000 INFO @ Tue, 16 Jun 2020 11:50:25: 36000000 INFO @ Tue, 16 Jun 2020 11:50:29: 44000000 INFO @ Tue, 16 Jun 2020 11:50:31: 40000000 INFO @ Tue, 16 Jun 2020 11:50:33: 37000000 INFO @ Tue, 16 Jun 2020 11:50:37: 45000000 INFO @ Tue, 16 Jun 2020 11:50:39: 41000000 INFO @ Tue, 16 Jun 2020 11:50:41: 38000000 INFO @ Tue, 16 Jun 2020 11:50:44: 46000000 INFO @ Tue, 16 Jun 2020 11:50:47: 42000000 INFO @ Tue, 16 Jun 2020 11:50:49: 39000000 INFO @ Tue, 16 Jun 2020 11:50:52: 47000000 INFO @ Tue, 16 Jun 2020 11:50:54: 43000000 INFO @ Tue, 16 Jun 2020 11:50:56: 40000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 11:51:00: 48000000 INFO @ Tue, 16 Jun 2020 11:51:02: 44000000 INFO @ Tue, 16 Jun 2020 11:51:04: 41000000 INFO @ Tue, 16 Jun 2020 11:51:07: 49000000 INFO @ Tue, 16 Jun 2020 11:51:10: 45000000 INFO @ Tue, 16 Jun 2020 11:51:11: 42000000 INFO @ Tue, 16 Jun 2020 11:51:15: 50000000 INFO @ Tue, 16 Jun 2020 11:51:17: 46000000 INFO @ Tue, 16 Jun 2020 11:51:19: 43000000 INFO @ Tue, 16 Jun 2020 11:51:23: 51000000 INFO @ Tue, 16 Jun 2020 11:51:25: 47000000 INFO @ Tue, 16 Jun 2020 11:51:27: 44000000 INFO @ Tue, 16 Jun 2020 11:51:30: 52000000 INFO @ Tue, 16 Jun 2020 11:51:33: 48000000 INFO @ Tue, 16 Jun 2020 11:51:34: 45000000 INFO @ Tue, 16 Jun 2020 11:51:38: 53000000 INFO @ Tue, 16 Jun 2020 11:51:41: 49000000 INFO @ Tue, 16 Jun 2020 11:51:42: 46000000 INFO @ Tue, 16 Jun 2020 11:51:45: 54000000 INFO @ Tue, 16 Jun 2020 11:51:49: 50000000 INFO @ Tue, 16 Jun 2020 11:51:49: 47000000 INFO @ Tue, 16 Jun 2020 11:51:53: 55000000 INFO @ Tue, 16 Jun 2020 11:51:57: 51000000 INFO @ Tue, 16 Jun 2020 11:51:57: 48000000 INFO @ Tue, 16 Jun 2020 11:52:01: 56000000 INFO @ Tue, 16 Jun 2020 11:52:05: 49000000 INFO @ Tue, 16 Jun 2020 11:52:05: 52000000 INFO @ Tue, 16 Jun 2020 11:52:08: 57000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 11:52:12: 53000000 INFO @ Tue, 16 Jun 2020 11:52:12: 50000000 INFO @ Tue, 16 Jun 2020 11:52:16: 58000000 INFO @ Tue, 16 Jun 2020 11:52:20: 54000000 INFO @ Tue, 16 Jun 2020 11:52:20: 51000000 INFO @ Tue, 16 Jun 2020 11:52:23: 59000000 INFO @ Tue, 16 Jun 2020 11:52:28: 55000000 INFO @ Tue, 16 Jun 2020 11:52:28: 52000000 INFO @ Tue, 16 Jun 2020 11:52:31: 60000000 INFO @ Tue, 16 Jun 2020 11:52:35: 56000000 INFO @ Tue, 16 Jun 2020 11:52:36: 53000000 INFO @ Tue, 16 Jun 2020 11:52:37: #1 tag size is determined as 150 bps INFO @ Tue, 16 Jun 2020 11:52:37: #1 tag size = 150 INFO @ Tue, 16 Jun 2020 11:52:37: #1 total tags in treatment: 21667315 INFO @ Tue, 16 Jun 2020 11:52:37: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 11:52:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 11:52:37: #1 tags after filtering in treatment: 17867308 INFO @ Tue, 16 Jun 2020 11:52:37: #1 Redundant rate of treatment: 0.18 INFO @ Tue, 16 Jun 2020 11:52:37: #1 finished! INFO @ Tue, 16 Jun 2020 11:52:37: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 11:52:37: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 11:52:38: #2 number of paired peaks: 244 WARNING @ Tue, 16 Jun 2020 11:52:38: Fewer paired peaks (244) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 244 pairs to build model! INFO @ Tue, 16 Jun 2020 11:52:38: start model_add_line... INFO @ Tue, 16 Jun 2020 11:52:39: start X-correlation... INFO @ Tue, 16 Jun 2020 11:52:39: end of X-cor INFO @ Tue, 16 Jun 2020 11:52:39: #2 finished! INFO @ Tue, 16 Jun 2020 11:52:39: #2 predicted fragment length is 212 bps INFO @ Tue, 16 Jun 2020 11:52:39: #2 alternative fragment length(s) may be 4,212 bps INFO @ Tue, 16 Jun 2020 11:52:39: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX6619545/SRX6619545.05_model.r WARNING @ Tue, 16 Jun 2020 11:52:39: #2 Since the d (212) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 11:52:39: #2 You may need to consider one of the other alternative d(s): 4,212 WARNING @ Tue, 16 Jun 2020 11:52:39: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 11:52:39: #3 Call peaks... INFO @ Tue, 16 Jun 2020 11:52:39: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 11:52:43: 57000000 INFO @ Tue, 16 Jun 2020 11:52:43: 54000000 INFO @ Tue, 16 Jun 2020 11:52:50: 58000000 INFO @ Tue, 16 Jun 2020 11:52:51: 55000000 INFO @ Tue, 16 Jun 2020 11:52:59: 59000000 INFO @ Tue, 16 Jun 2020 11:52:59: 56000000 INFO @ Tue, 16 Jun 2020 11:53:06: 57000000 INFO @ Tue, 16 Jun 2020 11:53:07: 60000000 INFO @ Tue, 16 Jun 2020 11:53:12: #1 tag size is determined as 150 bps INFO @ Tue, 16 Jun 2020 11:53:12: #1 tag size = 150 INFO @ Tue, 16 Jun 2020 11:53:12: #1 total tags in treatment: 21667315 INFO @ Tue, 16 Jun 2020 11:53:12: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 11:53:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 11:53:12: #1 tags after filtering in treatment: 17867308 INFO @ Tue, 16 Jun 2020 11:53:12: #1 Redundant rate of treatment: 0.18 INFO @ Tue, 16 Jun 2020 11:53:12: #1 finished! INFO @ Tue, 16 Jun 2020 11:53:12: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 11:53:12: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 11:53:14: #2 number of paired peaks: 244 WARNING @ Tue, 16 Jun 2020 11:53:14: Fewer paired peaks (244) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 244 pairs to build model! INFO @ Tue, 16 Jun 2020 11:53:14: start model_add_line... INFO @ Tue, 16 Jun 2020 11:53:14: 58000000 INFO @ Tue, 16 Jun 2020 11:53:14: start X-correlation... INFO @ Tue, 16 Jun 2020 11:53:14: end of X-cor INFO @ Tue, 16 Jun 2020 11:53:14: #2 finished! INFO @ Tue, 16 Jun 2020 11:53:14: #2 predicted fragment length is 212 bps INFO @ Tue, 16 Jun 2020 11:53:14: #2 alternative fragment length(s) may be 4,212 bps INFO @ Tue, 16 Jun 2020 11:53:14: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX6619545/SRX6619545.10_model.r WARNING @ Tue, 16 Jun 2020 11:53:14: #2 Since the d (212) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 11:53:14: #2 You may need to consider one of the other alternative d(s): 4,212 WARNING @ Tue, 16 Jun 2020 11:53:14: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 11:53:14: #3 Call peaks... INFO @ Tue, 16 Jun 2020 11:53:14: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 11:53:17: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 11:53:21: 59000000 INFO @ Tue, 16 Jun 2020 11:53:29: 60000000 INFO @ Tue, 16 Jun 2020 11:53:34: #1 tag size is determined as 150 bps INFO @ Tue, 16 Jun 2020 11:53:34: #1 tag size = 150 INFO @ Tue, 16 Jun 2020 11:53:34: #1 total tags in treatment: 21667315 INFO @ Tue, 16 Jun 2020 11:53:34: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 11:53:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 11:53:35: #1 tags after filtering in treatment: 17867308 INFO @ Tue, 16 Jun 2020 11:53:35: #1 Redundant rate of treatment: 0.18 INFO @ Tue, 16 Jun 2020 11:53:35: #1 finished! INFO @ Tue, 16 Jun 2020 11:53:35: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 11:53:35: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 11:53:36: #2 number of paired peaks: 244 WARNING @ Tue, 16 Jun 2020 11:53:36: Fewer paired peaks (244) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 244 pairs to build model! INFO @ Tue, 16 Jun 2020 11:53:36: start model_add_line... INFO @ Tue, 16 Jun 2020 11:53:36: start X-correlation... INFO @ Tue, 16 Jun 2020 11:53:36: end of X-cor INFO @ Tue, 16 Jun 2020 11:53:36: #2 finished! INFO @ Tue, 16 Jun 2020 11:53:36: #2 predicted fragment length is 212 bps INFO @ Tue, 16 Jun 2020 11:53:36: #2 alternative fragment length(s) may be 4,212 bps INFO @ Tue, 16 Jun 2020 11:53:36: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX6619545/SRX6619545.20_model.r WARNING @ Tue, 16 Jun 2020 11:53:36: #2 Since the d (212) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 11:53:36: #2 You may need to consider one of the other alternative d(s): 4,212 WARNING @ Tue, 16 Jun 2020 11:53:36: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 11:53:36: #3 Call peaks... INFO @ Tue, 16 Jun 2020 11:53:36: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 11:53:37: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX6619545/SRX6619545.05_peaks.xls INFO @ Tue, 16 Jun 2020 11:53:38: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX6619545/SRX6619545.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 11:53:38: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX6619545/SRX6619545.05_summits.bed INFO @ Tue, 16 Jun 2020 11:53:38: Done! pass1 - making usageList (7 chroms): 4 millis pass2 - checking and writing primary data (28441 records, 4 fields): 27 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 11:53:53: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 11:54:13: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX6619545/SRX6619545.10_peaks.xls INFO @ Tue, 16 Jun 2020 11:54:13: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX6619545/SRX6619545.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 11:54:13: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX6619545/SRX6619545.10_summits.bed INFO @ Tue, 16 Jun 2020 11:54:13: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (14652 records, 4 fields): 15 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 11:54:15: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 11:54:33: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX6619545/SRX6619545.20_peaks.xls INFO @ Tue, 16 Jun 2020 11:54:33: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX6619545/SRX6619545.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 11:54:33: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX6619545/SRX6619545.20_summits.bed INFO @ Tue, 16 Jun 2020 11:54:33: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (2389 records, 4 fields): 3 millis CompletedMACS2peakCalling