Job ID = 6368822 SRX = SRX5985676 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-16T00:25:50 prefetch.2.10.7: 1) Downloading 'SRR9214981'... 2020-06-16T00:25:50 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:32:51 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:32:51 prefetch.2.10.7: 1) 'SRR9214981' was downloaded successfully Read 52868835 spots for SRR9214981/SRR9214981.sra Written 52868835 spots for SRR9214981/SRR9214981.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:32 52868835 reads; of these: 52868835 (100.00%) were unpaired; of these: 5363066 (10.14%) aligned 0 times 42981445 (81.30%) aligned exactly 1 time 4524324 (8.56%) aligned >1 times 89.86% overall alignment rate Time searching: 00:15:32 Overall time: 00:15:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 14009413 / 47505769 = 0.2949 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:11:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX5985676/SRX5985676.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX5985676/SRX5985676.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX5985676/SRX5985676.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX5985676/SRX5985676.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:11:01: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:11:01: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:11:07: 1000000 INFO @ Tue, 16 Jun 2020 10:11:13: 2000000 INFO @ Tue, 16 Jun 2020 10:11:18: 3000000 INFO @ Tue, 16 Jun 2020 10:11:24: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:11:29: 5000000 INFO @ Tue, 16 Jun 2020 10:11:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX5985676/SRX5985676.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX5985676/SRX5985676.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX5985676/SRX5985676.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX5985676/SRX5985676.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:11:31: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:11:31: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:11:35: 6000000 INFO @ Tue, 16 Jun 2020 10:11:38: 1000000 INFO @ Tue, 16 Jun 2020 10:11:42: 7000000 INFO @ Tue, 16 Jun 2020 10:11:44: 2000000 INFO @ Tue, 16 Jun 2020 10:11:48: 8000000 INFO @ Tue, 16 Jun 2020 10:11:50: 3000000 INFO @ Tue, 16 Jun 2020 10:11:54: 9000000 INFO @ Tue, 16 Jun 2020 10:11:57: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:12:00: 10000000 INFO @ Tue, 16 Jun 2020 10:12:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX5985676/SRX5985676.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX5985676/SRX5985676.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX5985676/SRX5985676.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX5985676/SRX5985676.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:12:01: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:12:01: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:12:03: 5000000 INFO @ Tue, 16 Jun 2020 10:12:06: 11000000 INFO @ Tue, 16 Jun 2020 10:12:07: 1000000 INFO @ Tue, 16 Jun 2020 10:12:09: 6000000 INFO @ Tue, 16 Jun 2020 10:12:12: 12000000 INFO @ Tue, 16 Jun 2020 10:12:13: 2000000 INFO @ Tue, 16 Jun 2020 10:12:16: 7000000 INFO @ Tue, 16 Jun 2020 10:12:18: 3000000 INFO @ Tue, 16 Jun 2020 10:12:19: 13000000 INFO @ Tue, 16 Jun 2020 10:12:22: 8000000 INFO @ Tue, 16 Jun 2020 10:12:24: 4000000 INFO @ Tue, 16 Jun 2020 10:12:25: 14000000 INFO @ Tue, 16 Jun 2020 10:12:28: 9000000 INFO @ Tue, 16 Jun 2020 10:12:30: 5000000 INFO @ Tue, 16 Jun 2020 10:12:31: 15000000 INFO @ Tue, 16 Jun 2020 10:12:34: 10000000 INFO @ Tue, 16 Jun 2020 10:12:36: 6000000 INFO @ Tue, 16 Jun 2020 10:12:37: 16000000 INFO @ Tue, 16 Jun 2020 10:12:41: 11000000 INFO @ Tue, 16 Jun 2020 10:12:42: 7000000 INFO @ Tue, 16 Jun 2020 10:12:43: 17000000 INFO @ Tue, 16 Jun 2020 10:12:47: 12000000 INFO @ Tue, 16 Jun 2020 10:12:47: 8000000 INFO @ Tue, 16 Jun 2020 10:12:50: 18000000 INFO @ Tue, 16 Jun 2020 10:12:53: 9000000 INFO @ Tue, 16 Jun 2020 10:12:53: 13000000 INFO @ Tue, 16 Jun 2020 10:12:56: 19000000 INFO @ Tue, 16 Jun 2020 10:12:59: 10000000 INFO @ Tue, 16 Jun 2020 10:12:59: 14000000 INFO @ Tue, 16 Jun 2020 10:13:02: 20000000 INFO @ Tue, 16 Jun 2020 10:13:05: 11000000 INFO @ Tue, 16 Jun 2020 10:13:06: 15000000 INFO @ Tue, 16 Jun 2020 10:13:08: 21000000 INFO @ Tue, 16 Jun 2020 10:13:11: 12000000 INFO @ Tue, 16 Jun 2020 10:13:12: 16000000 INFO @ Tue, 16 Jun 2020 10:13:14: 22000000 INFO @ Tue, 16 Jun 2020 10:13:16: 13000000 INFO @ Tue, 16 Jun 2020 10:13:18: 17000000 INFO @ Tue, 16 Jun 2020 10:13:21: 23000000 INFO @ Tue, 16 Jun 2020 10:13:22: 14000000 INFO @ Tue, 16 Jun 2020 10:13:24: 18000000 INFO @ Tue, 16 Jun 2020 10:13:27: 24000000 INFO @ Tue, 16 Jun 2020 10:13:28: 15000000 INFO @ Tue, 16 Jun 2020 10:13:31: 19000000 INFO @ Tue, 16 Jun 2020 10:13:33: 25000000 INFO @ Tue, 16 Jun 2020 10:13:34: 16000000 INFO @ Tue, 16 Jun 2020 10:13:37: 20000000 INFO @ Tue, 16 Jun 2020 10:13:39: 26000000 INFO @ Tue, 16 Jun 2020 10:13:39: 17000000 INFO @ Tue, 16 Jun 2020 10:13:43: 21000000 INFO @ Tue, 16 Jun 2020 10:13:45: 18000000 INFO @ Tue, 16 Jun 2020 10:13:46: 27000000 INFO @ Tue, 16 Jun 2020 10:13:49: 22000000 INFO @ Tue, 16 Jun 2020 10:13:51: 19000000 INFO @ Tue, 16 Jun 2020 10:13:52: 28000000 INFO @ Tue, 16 Jun 2020 10:13:55: 23000000 INFO @ Tue, 16 Jun 2020 10:13:57: 20000000 INFO @ Tue, 16 Jun 2020 10:13:58: 29000000 INFO @ Tue, 16 Jun 2020 10:14:02: 24000000 INFO @ Tue, 16 Jun 2020 10:14:02: 21000000 INFO @ Tue, 16 Jun 2020 10:14:05: 30000000 INFO @ Tue, 16 Jun 2020 10:14:08: 22000000 INFO @ Tue, 16 Jun 2020 10:14:08: 25000000 INFO @ Tue, 16 Jun 2020 10:14:11: 31000000 INFO @ Tue, 16 Jun 2020 10:14:14: 23000000 INFO @ Tue, 16 Jun 2020 10:14:15: 26000000 INFO @ Tue, 16 Jun 2020 10:14:18: 32000000 INFO @ Tue, 16 Jun 2020 10:14:20: 24000000 INFO @ Tue, 16 Jun 2020 10:14:21: 27000000 INFO @ Tue, 16 Jun 2020 10:14:25: 33000000 INFO @ Tue, 16 Jun 2020 10:14:26: 25000000 INFO @ Tue, 16 Jun 2020 10:14:28: 28000000 INFO @ Tue, 16 Jun 2020 10:14:29: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 10:14:29: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 10:14:29: #1 total tags in treatment: 33496356 INFO @ Tue, 16 Jun 2020 10:14:29: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:14:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:14:29: #1 tags after filtering in treatment: 33496356 INFO @ Tue, 16 Jun 2020 10:14:29: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:14:29: #1 finished! INFO @ Tue, 16 Jun 2020 10:14:29: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:14:29: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:14:31: 26000000 INFO @ Tue, 16 Jun 2020 10:14:31: #2 number of paired peaks: 21 WARNING @ Tue, 16 Jun 2020 10:14:31: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:14:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX5985676/SRX5985676.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5985676/SRX5985676.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5985676/SRX5985676.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5985676/SRX5985676.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:14:34: 29000000 INFO @ Tue, 16 Jun 2020 10:14:37: 27000000 INFO @ Tue, 16 Jun 2020 10:14:41: 30000000 INFO @ Tue, 16 Jun 2020 10:14:43: 28000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 10:14:48: 31000000 INFO @ Tue, 16 Jun 2020 10:14:49: 29000000 INFO @ Tue, 16 Jun 2020 10:14:55: 30000000 INFO @ Tue, 16 Jun 2020 10:14:55: 32000000 INFO @ Tue, 16 Jun 2020 10:15:00: 31000000 INFO @ Tue, 16 Jun 2020 10:15:01: 33000000 INFO @ Tue, 16 Jun 2020 10:15:04: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 10:15:04: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 10:15:04: #1 total tags in treatment: 33496356 INFO @ Tue, 16 Jun 2020 10:15:04: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:15:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:15:05: #1 tags after filtering in treatment: 33496356 INFO @ Tue, 16 Jun 2020 10:15:05: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:15:05: #1 finished! INFO @ Tue, 16 Jun 2020 10:15:05: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:15:05: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:15:06: 32000000 INFO @ Tue, 16 Jun 2020 10:15:07: #2 number of paired peaks: 21 WARNING @ Tue, 16 Jun 2020 10:15:07: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:15:07: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX5985676/SRX5985676.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5985676/SRX5985676.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5985676/SRX5985676.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5985676/SRX5985676.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:15:12: 33000000 INFO @ Tue, 16 Jun 2020 10:15:15: #1 tag size is determined as 76 bps INFO @ Tue, 16 Jun 2020 10:15:15: #1 tag size = 76 INFO @ Tue, 16 Jun 2020 10:15:15: #1 total tags in treatment: 33496356 INFO @ Tue, 16 Jun 2020 10:15:15: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:15:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:15:16: #1 tags after filtering in treatment: 33496356 INFO @ Tue, 16 Jun 2020 10:15:16: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:15:16: #1 finished! INFO @ Tue, 16 Jun 2020 10:15:16: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:15:16: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:15:18: #2 number of paired peaks: 21 WARNING @ Tue, 16 Jun 2020 10:15:18: Too few paired peaks (21) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:15:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX5985676/SRX5985676.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5985676/SRX5985676.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5985676/SRX5985676.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5985676/SRX5985676.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。