Job ID = 6368758 SRX = SRX5545243 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-16T00:28:48 prefetch.2.10.7: 1) Downloading 'SRR8754487'... 2020-06-16T00:28:48 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:36:02 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:36:02 prefetch.2.10.7: 1) 'SRR8754487' was downloaded successfully 2020-06-16T00:36:02 prefetch.2.10.7: 'SRR8754487' has 0 unresolved dependencies Read 85576422 spots for SRR8754487/SRR8754487.sra Written 85576422 spots for SRR8754487/SRR8754487.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:18:22 85576422 reads; of these: 85576422 (100.00%) were unpaired; of these: 10958817 (12.81%) aligned 0 times 63413340 (74.10%) aligned exactly 1 time 11204265 (13.09%) aligned >1 times 87.19% overall alignment rate Time searching: 00:18:22 Overall time: 00:18:22 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 32 files... [bam_rmdupse_core] 27447715 / 74617605 = 0.3678 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:11:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX5545243/SRX5545243.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX5545243/SRX5545243.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX5545243/SRX5545243.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX5545243/SRX5545243.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:11:05: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:11:05: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:11:12: 1000000 INFO @ Tue, 16 Jun 2020 10:11:19: 2000000 INFO @ Tue, 16 Jun 2020 10:11:26: 3000000 INFO @ Tue, 16 Jun 2020 10:11:32: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:11:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX5545243/SRX5545243.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX5545243/SRX5545243.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX5545243/SRX5545243.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX5545243/SRX5545243.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:11:35: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:11:35: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:11:40: 5000000 INFO @ Tue, 16 Jun 2020 10:11:42: 1000000 INFO @ Tue, 16 Jun 2020 10:11:47: 6000000 INFO @ Tue, 16 Jun 2020 10:11:49: 2000000 INFO @ Tue, 16 Jun 2020 10:11:55: 7000000 INFO @ Tue, 16 Jun 2020 10:11:55: 3000000 INFO @ Tue, 16 Jun 2020 10:12:02: 4000000 INFO @ Tue, 16 Jun 2020 10:12:02: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:12:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX5545243/SRX5545243.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX5545243/SRX5545243.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX5545243/SRX5545243.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX5545243/SRX5545243.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:12:05: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:12:05: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:12:08: 5000000 INFO @ Tue, 16 Jun 2020 10:12:10: 9000000 INFO @ Tue, 16 Jun 2020 10:12:12: 1000000 INFO @ Tue, 16 Jun 2020 10:12:15: 6000000 INFO @ Tue, 16 Jun 2020 10:12:17: 10000000 INFO @ Tue, 16 Jun 2020 10:12:19: 2000000 INFO @ Tue, 16 Jun 2020 10:12:22: 7000000 INFO @ Tue, 16 Jun 2020 10:12:25: 11000000 INFO @ Tue, 16 Jun 2020 10:12:26: 3000000 INFO @ Tue, 16 Jun 2020 10:12:29: 8000000 INFO @ Tue, 16 Jun 2020 10:12:32: 12000000 INFO @ Tue, 16 Jun 2020 10:12:33: 4000000 INFO @ Tue, 16 Jun 2020 10:12:36: 9000000 INFO @ Tue, 16 Jun 2020 10:12:39: 5000000 INFO @ Tue, 16 Jun 2020 10:12:40: 13000000 INFO @ Tue, 16 Jun 2020 10:12:42: 10000000 INFO @ Tue, 16 Jun 2020 10:12:46: 6000000 INFO @ Tue, 16 Jun 2020 10:12:47: 14000000 INFO @ Tue, 16 Jun 2020 10:12:49: 11000000 INFO @ Tue, 16 Jun 2020 10:12:53: 7000000 INFO @ Tue, 16 Jun 2020 10:12:55: 15000000 INFO @ Tue, 16 Jun 2020 10:12:56: 12000000 INFO @ Tue, 16 Jun 2020 10:13:00: 8000000 INFO @ Tue, 16 Jun 2020 10:13:02: 16000000 INFO @ Tue, 16 Jun 2020 10:13:03: 13000000 INFO @ Tue, 16 Jun 2020 10:13:06: 9000000 INFO @ Tue, 16 Jun 2020 10:13:09: 14000000 INFO @ Tue, 16 Jun 2020 10:13:09: 17000000 INFO @ Tue, 16 Jun 2020 10:13:13: 10000000 INFO @ Tue, 16 Jun 2020 10:13:16: 15000000 INFO @ Tue, 16 Jun 2020 10:13:17: 18000000 INFO @ Tue, 16 Jun 2020 10:13:20: 11000000 INFO @ Tue, 16 Jun 2020 10:13:22: 16000000 INFO @ Tue, 16 Jun 2020 10:13:24: 19000000 INFO @ Tue, 16 Jun 2020 10:13:26: 12000000 INFO @ Tue, 16 Jun 2020 10:13:29: 17000000 INFO @ Tue, 16 Jun 2020 10:13:31: 20000000 INFO @ Tue, 16 Jun 2020 10:13:33: 13000000 INFO @ Tue, 16 Jun 2020 10:13:36: 18000000 INFO @ Tue, 16 Jun 2020 10:13:39: 21000000 INFO @ Tue, 16 Jun 2020 10:13:40: 14000000 INFO @ Tue, 16 Jun 2020 10:13:42: 19000000 INFO @ Tue, 16 Jun 2020 10:13:46: 22000000 INFO @ Tue, 16 Jun 2020 10:13:46: 15000000 INFO @ Tue, 16 Jun 2020 10:13:49: 20000000 INFO @ Tue, 16 Jun 2020 10:13:53: 16000000 INFO @ Tue, 16 Jun 2020 10:13:53: 23000000 INFO @ Tue, 16 Jun 2020 10:13:56: 21000000 INFO @ Tue, 16 Jun 2020 10:14:00: 17000000 INFO @ Tue, 16 Jun 2020 10:14:01: 24000000 INFO @ Tue, 16 Jun 2020 10:14:02: 22000000 INFO @ Tue, 16 Jun 2020 10:14:07: 18000000 INFO @ Tue, 16 Jun 2020 10:14:09: 25000000 INFO @ Tue, 16 Jun 2020 10:14:09: 23000000 INFO @ Tue, 16 Jun 2020 10:14:13: 19000000 INFO @ Tue, 16 Jun 2020 10:14:16: 24000000 INFO @ Tue, 16 Jun 2020 10:14:16: 26000000 INFO @ Tue, 16 Jun 2020 10:14:20: 20000000 INFO @ Tue, 16 Jun 2020 10:14:23: 25000000 INFO @ Tue, 16 Jun 2020 10:14:23: 27000000 INFO @ Tue, 16 Jun 2020 10:14:27: 21000000 INFO @ Tue, 16 Jun 2020 10:14:29: 26000000 INFO @ Tue, 16 Jun 2020 10:14:31: 28000000 INFO @ Tue, 16 Jun 2020 10:14:33: 22000000 INFO @ Tue, 16 Jun 2020 10:14:36: 27000000 INFO @ Tue, 16 Jun 2020 10:14:38: 29000000 INFO @ Tue, 16 Jun 2020 10:14:40: 23000000 INFO @ Tue, 16 Jun 2020 10:14:43: 28000000 INFO @ Tue, 16 Jun 2020 10:14:45: 30000000 INFO @ Tue, 16 Jun 2020 10:14:47: 24000000 INFO @ Tue, 16 Jun 2020 10:14:50: 29000000 INFO @ Tue, 16 Jun 2020 10:14:53: 31000000 INFO @ Tue, 16 Jun 2020 10:14:54: 25000000 INFO @ Tue, 16 Jun 2020 10:14:57: 30000000 INFO @ Tue, 16 Jun 2020 10:15:00: 32000000 INFO @ Tue, 16 Jun 2020 10:15:01: 26000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 10:15:04: 31000000 INFO @ Tue, 16 Jun 2020 10:15:08: 27000000 INFO @ Tue, 16 Jun 2020 10:15:08: 33000000 INFO @ Tue, 16 Jun 2020 10:15:11: 32000000 INFO @ Tue, 16 Jun 2020 10:15:15: 28000000 INFO @ Tue, 16 Jun 2020 10:15:15: 34000000 INFO @ Tue, 16 Jun 2020 10:15:18: 33000000 INFO @ Tue, 16 Jun 2020 10:15:21: 29000000 INFO @ Tue, 16 Jun 2020 10:15:23: 35000000 INFO @ Tue, 16 Jun 2020 10:15:24: 34000000 INFO @ Tue, 16 Jun 2020 10:15:28: 30000000 INFO @ Tue, 16 Jun 2020 10:15:30: 36000000 INFO @ Tue, 16 Jun 2020 10:15:31: 35000000 INFO @ Tue, 16 Jun 2020 10:15:35: 31000000 INFO @ Tue, 16 Jun 2020 10:15:38: 37000000 INFO @ Tue, 16 Jun 2020 10:15:38: 36000000 INFO @ Tue, 16 Jun 2020 10:15:42: 32000000 INFO @ Tue, 16 Jun 2020 10:15:45: 37000000 INFO @ Tue, 16 Jun 2020 10:15:45: 38000000 INFO @ Tue, 16 Jun 2020 10:15:49: 33000000 INFO @ Tue, 16 Jun 2020 10:15:52: 38000000 INFO @ Tue, 16 Jun 2020 10:15:52: 39000000 INFO @ Tue, 16 Jun 2020 10:15:56: 34000000 INFO @ Tue, 16 Jun 2020 10:15:59: 39000000 INFO @ Tue, 16 Jun 2020 10:16:00: 40000000 INFO @ Tue, 16 Jun 2020 10:16:03: 35000000 INFO @ Tue, 16 Jun 2020 10:16:05: 40000000 INFO @ Tue, 16 Jun 2020 10:16:07: 41000000 INFO @ Tue, 16 Jun 2020 10:16:10: 36000000 INFO @ Tue, 16 Jun 2020 10:16:12: 41000000 INFO @ Tue, 16 Jun 2020 10:16:14: 42000000 INFO @ Tue, 16 Jun 2020 10:16:17: 37000000 INFO @ Tue, 16 Jun 2020 10:16:19: 42000000 INFO @ Tue, 16 Jun 2020 10:16:22: 43000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 10:16:24: 38000000 INFO @ Tue, 16 Jun 2020 10:16:26: 43000000 INFO @ Tue, 16 Jun 2020 10:16:29: 44000000 INFO @ Tue, 16 Jun 2020 10:16:31: 39000000 INFO @ Tue, 16 Jun 2020 10:16:33: 44000000 INFO @ Tue, 16 Jun 2020 10:16:37: 45000000 INFO @ Tue, 16 Jun 2020 10:16:38: 40000000 INFO @ Tue, 16 Jun 2020 10:16:40: 45000000 INFO @ Tue, 16 Jun 2020 10:16:44: 46000000 INFO @ Tue, 16 Jun 2020 10:16:45: 41000000 INFO @ Tue, 16 Jun 2020 10:16:47: 46000000 INFO @ Tue, 16 Jun 2020 10:16:51: 47000000 INFO @ Tue, 16 Jun 2020 10:16:51: 42000000 INFO @ Tue, 16 Jun 2020 10:16:53: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 10:16:53: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 10:16:53: #1 total tags in treatment: 47169890 INFO @ Tue, 16 Jun 2020 10:16:53: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:16:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:16:53: 47000000 INFO @ Tue, 16 Jun 2020 10:16:54: #1 tags after filtering in treatment: 47169890 INFO @ Tue, 16 Jun 2020 10:16:54: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:16:54: #1 finished! INFO @ Tue, 16 Jun 2020 10:16:54: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:16:54: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:16:55: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 10:16:55: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 10:16:55: #1 total tags in treatment: 47169890 INFO @ Tue, 16 Jun 2020 10:16:55: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:16:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:16:56: #1 tags after filtering in treatment: 47169890 INFO @ Tue, 16 Jun 2020 10:16:56: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:16:56: #1 finished! INFO @ Tue, 16 Jun 2020 10:16:56: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:16:56: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:16:57: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 10:16:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:16:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX5545243/SRX5545243.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545243/SRX5545243.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545243/SRX5545243.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545243/SRX5545243.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:16:58: 43000000 INFO @ Tue, 16 Jun 2020 10:16:59: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 10:16:59: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:16:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX5545243/SRX5545243.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545243/SRX5545243.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545243/SRX5545243.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545243/SRX5545243.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:17:04: 44000000 INFO @ Tue, 16 Jun 2020 10:17:10: 45000000 INFO @ Tue, 16 Jun 2020 10:17:16: 46000000 INFO @ Tue, 16 Jun 2020 10:17:21: 47000000 INFO @ Tue, 16 Jun 2020 10:17:23: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 10:17:23: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 10:17:23: #1 total tags in treatment: 47169890 INFO @ Tue, 16 Jun 2020 10:17:23: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:17:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:17:23: #1 tags after filtering in treatment: 47169890 INFO @ Tue, 16 Jun 2020 10:17:23: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:17:23: #1 finished! INFO @ Tue, 16 Jun 2020 10:17:23: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:17:23: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:17:27: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 10:17:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:17:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX5545243/SRX5545243.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545243/SRX5545243.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545243/SRX5545243.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545243/SRX5545243.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling