Job ID = 6368754 SRX = SRX5545239 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-16T00:39:16 prefetch.2.10.7: 1) Downloading 'SRR8754479'... 2020-06-16T00:39:16 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:47:10 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:47:10 prefetch.2.10.7: 1) 'SRR8754479' was downloaded successfully 2020-06-16T00:47:10 prefetch.2.10.7: 'SRR8754479' has 0 unresolved dependencies Read 90296341 spots for SRR8754479/SRR8754479.sra Written 90296341 spots for SRR8754479/SRR8754479.sra 2020-06-16T00:53:05 prefetch.2.10.7: 1) Downloading 'SRR8754480'... 2020-06-16T00:53:05 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:55:53 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:55:53 prefetch.2.10.7: 1) 'SRR8754480' was downloaded successfully 2020-06-16T00:55:53 prefetch.2.10.7: 'SRR8754480' has 0 unresolved dependencies Read 54134320 spots for SRR8754480/SRR8754480.sra Written 54134320 spots for SRR8754480/SRR8754480.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:27:39 144430661 reads; of these: 144430661 (100.00%) were unpaired; of these: 41324911 (28.61%) aligned 0 times 85166123 (58.97%) aligned exactly 1 time 17939627 (12.42%) aligned >1 times 71.39% overall alignment rate Time searching: 00:27:39 Overall time: 00:27:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 44 files... [bam_rmdupse_core] 45144052 / 103105750 = 0.4378 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:48:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX5545239/SRX5545239.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX5545239/SRX5545239.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX5545239/SRX5545239.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX5545239/SRX5545239.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:48:12: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:48:12: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:48:18: 1000000 INFO @ Tue, 16 Jun 2020 10:48:24: 2000000 INFO @ Tue, 16 Jun 2020 10:48:29: 3000000 INFO @ Tue, 16 Jun 2020 10:48:35: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:48:41: 5000000 INFO @ Tue, 16 Jun 2020 10:48:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX5545239/SRX5545239.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX5545239/SRX5545239.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX5545239/SRX5545239.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX5545239/SRX5545239.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:48:42: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:48:42: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:48:47: 6000000 INFO @ Tue, 16 Jun 2020 10:48:48: 1000000 INFO @ Tue, 16 Jun 2020 10:48:54: 7000000 INFO @ Tue, 16 Jun 2020 10:48:55: 2000000 INFO @ Tue, 16 Jun 2020 10:49:00: 8000000 INFO @ Tue, 16 Jun 2020 10:49:02: 3000000 INFO @ Tue, 16 Jun 2020 10:49:06: 9000000 INFO @ Tue, 16 Jun 2020 10:49:08: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:49:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX5545239/SRX5545239.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX5545239/SRX5545239.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX5545239/SRX5545239.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX5545239/SRX5545239.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:49:12: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:49:12: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:49:13: 10000000 INFO @ Tue, 16 Jun 2020 10:49:15: 5000000 INFO @ Tue, 16 Jun 2020 10:49:19: 1000000 INFO @ Tue, 16 Jun 2020 10:49:19: 11000000 INFO @ Tue, 16 Jun 2020 10:49:22: 6000000 INFO @ Tue, 16 Jun 2020 10:49:26: 12000000 INFO @ Tue, 16 Jun 2020 10:49:26: 2000000 INFO @ Tue, 16 Jun 2020 10:49:29: 7000000 INFO @ Tue, 16 Jun 2020 10:49:33: 13000000 INFO @ Tue, 16 Jun 2020 10:49:33: 3000000 INFO @ Tue, 16 Jun 2020 10:49:35: 8000000 INFO @ Tue, 16 Jun 2020 10:49:39: 14000000 INFO @ Tue, 16 Jun 2020 10:49:41: 4000000 INFO @ Tue, 16 Jun 2020 10:49:42: 9000000 INFO @ Tue, 16 Jun 2020 10:49:46: 15000000 INFO @ Tue, 16 Jun 2020 10:49:48: 5000000 INFO @ Tue, 16 Jun 2020 10:49:49: 10000000 INFO @ Tue, 16 Jun 2020 10:49:53: 16000000 INFO @ Tue, 16 Jun 2020 10:49:55: 6000000 INFO @ Tue, 16 Jun 2020 10:49:56: 11000000 INFO @ Tue, 16 Jun 2020 10:50:00: 17000000 INFO @ Tue, 16 Jun 2020 10:50:02: 7000000 INFO @ Tue, 16 Jun 2020 10:50:02: 12000000 INFO @ Tue, 16 Jun 2020 10:50:06: 18000000 INFO @ Tue, 16 Jun 2020 10:50:09: 13000000 INFO @ Tue, 16 Jun 2020 10:50:09: 8000000 INFO @ Tue, 16 Jun 2020 10:50:13: 19000000 INFO @ Tue, 16 Jun 2020 10:50:16: 14000000 INFO @ Tue, 16 Jun 2020 10:50:16: 9000000 INFO @ Tue, 16 Jun 2020 10:50:20: 20000000 INFO @ Tue, 16 Jun 2020 10:50:23: 15000000 INFO @ Tue, 16 Jun 2020 10:50:24: 10000000 INFO @ Tue, 16 Jun 2020 10:50:26: 21000000 INFO @ Tue, 16 Jun 2020 10:50:29: 16000000 INFO @ Tue, 16 Jun 2020 10:50:31: 11000000 INFO @ Tue, 16 Jun 2020 10:50:33: 22000000 INFO @ Tue, 16 Jun 2020 10:50:36: 17000000 INFO @ Tue, 16 Jun 2020 10:50:38: 12000000 INFO @ Tue, 16 Jun 2020 10:50:40: 23000000 INFO @ Tue, 16 Jun 2020 10:50:43: 18000000 INFO @ Tue, 16 Jun 2020 10:50:45: 13000000 INFO @ Tue, 16 Jun 2020 10:50:46: 24000000 INFO @ Tue, 16 Jun 2020 10:50:50: 19000000 INFO @ Tue, 16 Jun 2020 10:50:52: 14000000 INFO @ Tue, 16 Jun 2020 10:50:53: 25000000 INFO @ Tue, 16 Jun 2020 10:50:56: 20000000 INFO @ Tue, 16 Jun 2020 10:50:59: 15000000 INFO @ Tue, 16 Jun 2020 10:51:00: 26000000 INFO @ Tue, 16 Jun 2020 10:51:03: 21000000 INFO @ Tue, 16 Jun 2020 10:51:06: 16000000 INFO @ Tue, 16 Jun 2020 10:51:07: 27000000 INFO @ Tue, 16 Jun 2020 10:51:10: 22000000 INFO @ Tue, 16 Jun 2020 10:51:13: 17000000 INFO @ Tue, 16 Jun 2020 10:51:13: 28000000 INFO @ Tue, 16 Jun 2020 10:51:17: 23000000 INFO @ Tue, 16 Jun 2020 10:51:20: 29000000 INFO @ Tue, 16 Jun 2020 10:51:20: 18000000 INFO @ Tue, 16 Jun 2020 10:51:23: 24000000 INFO @ Tue, 16 Jun 2020 10:51:27: 30000000 INFO @ Tue, 16 Jun 2020 10:51:28: 19000000 INFO @ Tue, 16 Jun 2020 10:51:30: 25000000 INFO @ Tue, 16 Jun 2020 10:51:33: 31000000 INFO @ Tue, 16 Jun 2020 10:51:35: 20000000 INFO @ Tue, 16 Jun 2020 10:51:37: 26000000 INFO @ Tue, 16 Jun 2020 10:51:40: 32000000 INFO @ Tue, 16 Jun 2020 10:51:42: 21000000 INFO @ Tue, 16 Jun 2020 10:51:44: 27000000 INFO @ Tue, 16 Jun 2020 10:51:47: 33000000 INFO @ Tue, 16 Jun 2020 10:51:49: 22000000 INFO @ Tue, 16 Jun 2020 10:51:50: 28000000 INFO @ Tue, 16 Jun 2020 10:51:53: 34000000 INFO @ Tue, 16 Jun 2020 10:51:56: 23000000 INFO @ Tue, 16 Jun 2020 10:51:57: 29000000 INFO @ Tue, 16 Jun 2020 10:52:00: 35000000 INFO @ Tue, 16 Jun 2020 10:52:03: 24000000 INFO @ Tue, 16 Jun 2020 10:52:04: 30000000 INFO @ Tue, 16 Jun 2020 10:52:07: 36000000 INFO @ Tue, 16 Jun 2020 10:52:10: 25000000 INFO @ Tue, 16 Jun 2020 10:52:10: 31000000 INFO @ Tue, 16 Jun 2020 10:52:13: 37000000 INFO @ Tue, 16 Jun 2020 10:52:17: 32000000 INFO @ Tue, 16 Jun 2020 10:52:17: 26000000 INFO @ Tue, 16 Jun 2020 10:52:20: 38000000 INFO @ Tue, 16 Jun 2020 10:52:24: 33000000 INFO @ Tue, 16 Jun 2020 10:52:24: 27000000 INFO @ Tue, 16 Jun 2020 10:52:27: 39000000 INFO @ Tue, 16 Jun 2020 10:52:31: 34000000 INFO @ Tue, 16 Jun 2020 10:52:32: 28000000 INFO @ Tue, 16 Jun 2020 10:52:33: 40000000 INFO @ Tue, 16 Jun 2020 10:52:37: 35000000 INFO @ Tue, 16 Jun 2020 10:52:39: 29000000 INFO @ Tue, 16 Jun 2020 10:52:40: 41000000 INFO @ Tue, 16 Jun 2020 10:52:44: 36000000 INFO @ Tue, 16 Jun 2020 10:52:46: 30000000 INFO @ Tue, 16 Jun 2020 10:52:47: 42000000 INFO @ Tue, 16 Jun 2020 10:52:51: 37000000 INFO @ Tue, 16 Jun 2020 10:52:53: 31000000 INFO @ Tue, 16 Jun 2020 10:52:53: 43000000 INFO @ Tue, 16 Jun 2020 10:52:58: 38000000 INFO @ Tue, 16 Jun 2020 10:53:00: 32000000 INFO @ Tue, 16 Jun 2020 10:53:00: 44000000 INFO @ Tue, 16 Jun 2020 10:53:04: 39000000 INFO @ Tue, 16 Jun 2020 10:53:07: 45000000 INFO @ Tue, 16 Jun 2020 10:53:07: 33000000 INFO @ Tue, 16 Jun 2020 10:53:11: 40000000 INFO @ Tue, 16 Jun 2020 10:53:14: 46000000 INFO @ Tue, 16 Jun 2020 10:53:14: 34000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 10:53:18: 41000000 INFO @ Tue, 16 Jun 2020 10:53:21: 47000000 INFO @ Tue, 16 Jun 2020 10:53:22: 35000000 INFO @ Tue, 16 Jun 2020 10:53:25: 42000000 INFO @ Tue, 16 Jun 2020 10:53:28: 48000000 INFO @ Tue, 16 Jun 2020 10:53:29: 36000000 INFO @ Tue, 16 Jun 2020 10:53:32: 43000000 INFO @ Tue, 16 Jun 2020 10:53:34: 49000000 INFO @ Tue, 16 Jun 2020 10:53:36: 37000000 INFO @ Tue, 16 Jun 2020 10:53:38: 44000000 INFO @ Tue, 16 Jun 2020 10:53:41: 50000000 INFO @ Tue, 16 Jun 2020 10:53:43: 38000000 INFO @ Tue, 16 Jun 2020 10:53:45: 45000000 INFO @ Tue, 16 Jun 2020 10:53:48: 51000000 INFO @ Tue, 16 Jun 2020 10:53:50: 39000000 INFO @ Tue, 16 Jun 2020 10:53:52: 46000000 INFO @ Tue, 16 Jun 2020 10:53:54: 52000000 INFO @ Tue, 16 Jun 2020 10:53:57: 40000000 INFO @ Tue, 16 Jun 2020 10:53:59: 47000000 INFO @ Tue, 16 Jun 2020 10:54:01: 53000000 INFO @ Tue, 16 Jun 2020 10:54:04: 41000000 INFO @ Tue, 16 Jun 2020 10:54:05: 48000000 INFO @ Tue, 16 Jun 2020 10:54:08: 54000000 INFO @ Tue, 16 Jun 2020 10:54:11: 42000000 INFO @ Tue, 16 Jun 2020 10:54:12: 49000000 INFO @ Tue, 16 Jun 2020 10:54:14: 55000000 INFO @ Tue, 16 Jun 2020 10:54:18: 43000000 INFO @ Tue, 16 Jun 2020 10:54:19: 50000000 INFO @ Tue, 16 Jun 2020 10:54:21: 56000000 INFO @ Tue, 16 Jun 2020 10:54:26: 44000000 INFO @ Tue, 16 Jun 2020 10:54:26: 51000000 INFO @ Tue, 16 Jun 2020 10:54:28: 57000000 INFO @ Tue, 16 Jun 2020 10:54:33: 52000000 INFO @ Tue, 16 Jun 2020 10:54:33: 45000000 INFO @ Tue, 16 Jun 2020 10:54:35: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 10:54:35: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 10:54:35: #1 total tags in treatment: 57961698 INFO @ Tue, 16 Jun 2020 10:54:35: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:54:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:54:36: #1 tags after filtering in treatment: 57961698 INFO @ Tue, 16 Jun 2020 10:54:36: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:54:36: #1 finished! INFO @ Tue, 16 Jun 2020 10:54:36: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:54:36: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:54:39: 53000000 INFO @ Tue, 16 Jun 2020 10:54:39: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 10:54:39: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:54:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX5545239/SRX5545239.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545239/SRX5545239.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545239/SRX5545239.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545239/SRX5545239.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:54:40: 46000000 INFO @ Tue, 16 Jun 2020 10:54:45: 54000000 INFO @ Tue, 16 Jun 2020 10:54:47: 47000000 INFO @ Tue, 16 Jun 2020 10:54:51: 55000000 INFO @ Tue, 16 Jun 2020 10:54:54: 48000000 INFO @ Tue, 16 Jun 2020 10:54:57: 56000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 10:55:01: 49000000 INFO @ Tue, 16 Jun 2020 10:55:03: 57000000 INFO @ Tue, 16 Jun 2020 10:55:09: 50000000 INFO @ Tue, 16 Jun 2020 10:55:10: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 10:55:10: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 10:55:10: #1 total tags in treatment: 57961698 INFO @ Tue, 16 Jun 2020 10:55:10: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:55:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:55:11: #1 tags after filtering in treatment: 57961698 INFO @ Tue, 16 Jun 2020 10:55:11: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:55:11: #1 finished! INFO @ Tue, 16 Jun 2020 10:55:11: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:55:11: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:55:15: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 10:55:15: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:55:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX5545239/SRX5545239.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545239/SRX5545239.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545239/SRX5545239.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545239/SRX5545239.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:55:16: 51000000 INFO @ Tue, 16 Jun 2020 10:55:23: 52000000 INFO @ Tue, 16 Jun 2020 10:55:30: 53000000 INFO @ Tue, 16 Jun 2020 10:55:36: 54000000 INFO @ Tue, 16 Jun 2020 10:55:43: 55000000 INFO @ Tue, 16 Jun 2020 10:55:50: 56000000 INFO @ Tue, 16 Jun 2020 10:55:57: 57000000 INFO @ Tue, 16 Jun 2020 10:56:04: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 10:56:04: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 10:56:04: #1 total tags in treatment: 57961698 INFO @ Tue, 16 Jun 2020 10:56:04: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:56:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:56:06: #1 tags after filtering in treatment: 57961698 INFO @ Tue, 16 Jun 2020 10:56:06: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:56:06: #1 finished! INFO @ Tue, 16 Jun 2020 10:56:06: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:56:06: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:56:09: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 10:56:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:56:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX5545239/SRX5545239.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545239/SRX5545239.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545239/SRX5545239.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545239/SRX5545239.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling