Job ID = 6368752 SRX = SRX5545237 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-16T00:32:55 prefetch.2.10.7: 1) Downloading 'SRR8754475'... 2020-06-16T00:32:55 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:35:19 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:35:19 prefetch.2.10.7: 1) 'SRR8754475' was downloaded successfully 2020-06-16T00:35:19 prefetch.2.10.7: 'SRR8754475' has 0 unresolved dependencies Read 39096439 spots for SRR8754475/SRR8754475.sra Written 39096439 spots for SRR8754475/SRR8754475.sra 2020-06-16T00:37:37 prefetch.2.10.7: 1) Downloading 'SRR8754476'... 2020-06-16T00:37:37 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:41:30 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:41:30 prefetch.2.10.7: 1) 'SRR8754476' was downloaded successfully 2020-06-16T00:41:30 prefetch.2.10.7: 'SRR8754476' has 0 unresolved dependencies Read 50323522 spots for SRR8754476/SRR8754476.sra Written 50323522 spots for SRR8754476/SRR8754476.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:16:38 89419961 reads; of these: 89419961 (100.00%) were unpaired; of these: 28304925 (31.65%) aligned 0 times 50405498 (56.37%) aligned exactly 1 time 10709538 (11.98%) aligned >1 times 68.35% overall alignment rate Time searching: 00:16:39 Overall time: 00:16:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 25759645 / 61115036 = 0.4215 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:11:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX5545237/SRX5545237.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX5545237/SRX5545237.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX5545237/SRX5545237.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX5545237/SRX5545237.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:11:31: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:11:31: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:11:36: 1000000 INFO @ Tue, 16 Jun 2020 10:11:41: 2000000 INFO @ Tue, 16 Jun 2020 10:11:46: 3000000 INFO @ Tue, 16 Jun 2020 10:11:51: 4000000 INFO @ Tue, 16 Jun 2020 10:11:57: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:12:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX5545237/SRX5545237.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX5545237/SRX5545237.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX5545237/SRX5545237.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX5545237/SRX5545237.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:12:00: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:12:00: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:12:02: 6000000 INFO @ Tue, 16 Jun 2020 10:12:06: 1000000 INFO @ Tue, 16 Jun 2020 10:12:07: 7000000 INFO @ Tue, 16 Jun 2020 10:12:11: 2000000 INFO @ Tue, 16 Jun 2020 10:12:12: 8000000 INFO @ Tue, 16 Jun 2020 10:12:17: 3000000 INFO @ Tue, 16 Jun 2020 10:12:18: 9000000 INFO @ Tue, 16 Jun 2020 10:12:22: 4000000 INFO @ Tue, 16 Jun 2020 10:12:23: 10000000 INFO @ Tue, 16 Jun 2020 10:12:28: 5000000 INFO @ Tue, 16 Jun 2020 10:12:28: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:12:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX5545237/SRX5545237.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX5545237/SRX5545237.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX5545237/SRX5545237.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX5545237/SRX5545237.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:12:30: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:12:30: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:12:33: 6000000 INFO @ Tue, 16 Jun 2020 10:12:34: 12000000 INFO @ Tue, 16 Jun 2020 10:12:36: 1000000 INFO @ Tue, 16 Jun 2020 10:12:38: 7000000 INFO @ Tue, 16 Jun 2020 10:12:39: 13000000 INFO @ Tue, 16 Jun 2020 10:12:42: 2000000 INFO @ Tue, 16 Jun 2020 10:12:44: 8000000 INFO @ Tue, 16 Jun 2020 10:12:45: 14000000 INFO @ Tue, 16 Jun 2020 10:12:47: 3000000 INFO @ Tue, 16 Jun 2020 10:12:49: 9000000 INFO @ Tue, 16 Jun 2020 10:12:50: 15000000 INFO @ Tue, 16 Jun 2020 10:12:53: 4000000 INFO @ Tue, 16 Jun 2020 10:12:55: 10000000 INFO @ Tue, 16 Jun 2020 10:12:56: 16000000 INFO @ Tue, 16 Jun 2020 10:12:58: 5000000 INFO @ Tue, 16 Jun 2020 10:13:00: 11000000 INFO @ Tue, 16 Jun 2020 10:13:01: 17000000 INFO @ Tue, 16 Jun 2020 10:13:04: 6000000 INFO @ Tue, 16 Jun 2020 10:13:06: 12000000 INFO @ Tue, 16 Jun 2020 10:13:07: 18000000 INFO @ Tue, 16 Jun 2020 10:13:09: 7000000 INFO @ Tue, 16 Jun 2020 10:13:11: 13000000 INFO @ Tue, 16 Jun 2020 10:13:12: 19000000 INFO @ Tue, 16 Jun 2020 10:13:14: 8000000 INFO @ Tue, 16 Jun 2020 10:13:17: 14000000 INFO @ Tue, 16 Jun 2020 10:13:18: 20000000 INFO @ Tue, 16 Jun 2020 10:13:20: 9000000 INFO @ Tue, 16 Jun 2020 10:13:22: 15000000 INFO @ Tue, 16 Jun 2020 10:13:23: 21000000 INFO @ Tue, 16 Jun 2020 10:13:25: 10000000 INFO @ Tue, 16 Jun 2020 10:13:28: 16000000 INFO @ Tue, 16 Jun 2020 10:13:29: 22000000 INFO @ Tue, 16 Jun 2020 10:13:31: 11000000 INFO @ Tue, 16 Jun 2020 10:13:33: 17000000 INFO @ Tue, 16 Jun 2020 10:13:34: 23000000 INFO @ Tue, 16 Jun 2020 10:13:36: 12000000 INFO @ Tue, 16 Jun 2020 10:13:38: 18000000 INFO @ Tue, 16 Jun 2020 10:13:39: 24000000 INFO @ Tue, 16 Jun 2020 10:13:42: 13000000 INFO @ Tue, 16 Jun 2020 10:13:44: 19000000 INFO @ Tue, 16 Jun 2020 10:13:45: 25000000 INFO @ Tue, 16 Jun 2020 10:13:48: 14000000 INFO @ Tue, 16 Jun 2020 10:13:49: 20000000 INFO @ Tue, 16 Jun 2020 10:13:50: 26000000 INFO @ Tue, 16 Jun 2020 10:13:54: 15000000 INFO @ Tue, 16 Jun 2020 10:13:55: 21000000 INFO @ Tue, 16 Jun 2020 10:13:56: 27000000 INFO @ Tue, 16 Jun 2020 10:13:59: 16000000 INFO @ Tue, 16 Jun 2020 10:14:00: 22000000 INFO @ Tue, 16 Jun 2020 10:14:01: 28000000 INFO @ Tue, 16 Jun 2020 10:14:05: 17000000 INFO @ Tue, 16 Jun 2020 10:14:06: 23000000 INFO @ Tue, 16 Jun 2020 10:14:07: 29000000 INFO @ Tue, 16 Jun 2020 10:14:10: 18000000 INFO @ Tue, 16 Jun 2020 10:14:11: 24000000 INFO @ Tue, 16 Jun 2020 10:14:12: 30000000 INFO @ Tue, 16 Jun 2020 10:14:16: 19000000 INFO @ Tue, 16 Jun 2020 10:14:17: 25000000 INFO @ Tue, 16 Jun 2020 10:14:18: 31000000 INFO @ Tue, 16 Jun 2020 10:14:21: 20000000 INFO @ Tue, 16 Jun 2020 10:14:22: 26000000 INFO @ Tue, 16 Jun 2020 10:14:24: 32000000 INFO @ Tue, 16 Jun 2020 10:14:27: 21000000 INFO @ Tue, 16 Jun 2020 10:14:28: 27000000 INFO @ Tue, 16 Jun 2020 10:14:29: 33000000 INFO @ Tue, 16 Jun 2020 10:14:33: 22000000 INFO @ Tue, 16 Jun 2020 10:14:33: 28000000 INFO @ Tue, 16 Jun 2020 10:14:34: 34000000 INFO @ Tue, 16 Jun 2020 10:14:38: 23000000 INFO @ Tue, 16 Jun 2020 10:14:39: 29000000 INFO @ Tue, 16 Jun 2020 10:14:40: 35000000 INFO @ Tue, 16 Jun 2020 10:14:42: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 10:14:42: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 10:14:42: #1 total tags in treatment: 35355391 INFO @ Tue, 16 Jun 2020 10:14:42: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:14:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:14:43: #1 tags after filtering in treatment: 35355391 INFO @ Tue, 16 Jun 2020 10:14:43: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:14:43: #1 finished! INFO @ Tue, 16 Jun 2020 10:14:43: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:14:43: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:14:44: 24000000 INFO @ Tue, 16 Jun 2020 10:14:44: 30000000 INFO @ Tue, 16 Jun 2020 10:14:45: #2 number of paired peaks: 48 WARNING @ Tue, 16 Jun 2020 10:14:45: Too few paired peaks (48) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:14:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX5545237/SRX5545237.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545237/SRX5545237.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545237/SRX5545237.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545237/SRX5545237.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:14:50: 25000000 INFO @ Tue, 16 Jun 2020 10:14:50: 31000000 INFO @ Tue, 16 Jun 2020 10:14:55: 26000000 INFO @ Tue, 16 Jun 2020 10:14:55: 32000000 INFO @ Tue, 16 Jun 2020 10:15:01: 27000000 INFO @ Tue, 16 Jun 2020 10:15:01: 33000000 INFO @ Tue, 16 Jun 2020 10:15:06: 34000000 INFO @ Tue, 16 Jun 2020 10:15:06: 28000000 INFO @ Tue, 16 Jun 2020 10:15:12: 35000000 INFO @ Tue, 16 Jun 2020 10:15:12: 29000000 INFO @ Tue, 16 Jun 2020 10:15:14: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 10:15:14: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 10:15:14: #1 total tags in treatment: 35355391 INFO @ Tue, 16 Jun 2020 10:15:14: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:15:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:15:14: #1 tags after filtering in treatment: 35355391 INFO @ Tue, 16 Jun 2020 10:15:14: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:15:14: #1 finished! INFO @ Tue, 16 Jun 2020 10:15:14: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:15:14: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:15:17: #2 number of paired peaks: 48 WARNING @ Tue, 16 Jun 2020 10:15:17: Too few paired peaks (48) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:15:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX5545237/SRX5545237.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545237/SRX5545237.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545237/SRX5545237.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545237/SRX5545237.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:15:17: 30000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 10:15:23: 31000000 INFO @ Tue, 16 Jun 2020 10:15:28: 32000000 INFO @ Tue, 16 Jun 2020 10:15:34: 33000000 INFO @ Tue, 16 Jun 2020 10:15:39: 34000000 INFO @ Tue, 16 Jun 2020 10:15:44: 35000000 INFO @ Tue, 16 Jun 2020 10:15:46: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 10:15:46: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 10:15:46: #1 total tags in treatment: 35355391 INFO @ Tue, 16 Jun 2020 10:15:46: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:15:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:15:46: #1 tags after filtering in treatment: 35355391 INFO @ Tue, 16 Jun 2020 10:15:46: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:15:46: #1 finished! INFO @ Tue, 16 Jun 2020 10:15:46: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:15:46: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:15:49: #2 number of paired peaks: 48 WARNING @ Tue, 16 Jun 2020 10:15:49: Too few paired peaks (48) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:15:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX5545237/SRX5545237.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545237/SRX5545237.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545237/SRX5545237.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5545237/SRX5545237.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。