Job ID = 6368750 SRX = SRX5402773 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-16T00:33:44 prefetch.2.10.7: 1) Downloading 'SRR8603007'... 2020-06-16T00:33:44 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:37:50 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:37:50 prefetch.2.10.7: 1) 'SRR8603007' was downloaded successfully Read 34727185 spots for SRR8603007/SRR8603007.sra Written 34727185 spots for SRR8603007/SRR8603007.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:32 34727185 reads; of these: 34727185 (100.00%) were unpaired; of these: 2264096 (6.52%) aligned 0 times 26996025 (77.74%) aligned exactly 1 time 5467064 (15.74%) aligned >1 times 93.48% overall alignment rate Time searching: 00:07:32 Overall time: 00:07:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 4737127 / 32463089 = 0.1459 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:54:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX5402773/SRX5402773.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX5402773/SRX5402773.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX5402773/SRX5402773.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX5402773/SRX5402773.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:54:40: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:54:40: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:54:44: 1000000 INFO @ Tue, 16 Jun 2020 09:54:49: 2000000 INFO @ Tue, 16 Jun 2020 09:54:54: 3000000 INFO @ Tue, 16 Jun 2020 09:54:59: 4000000 INFO @ Tue, 16 Jun 2020 09:55:04: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:55:09: 6000000 INFO @ Tue, 16 Jun 2020 09:55:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX5402773/SRX5402773.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX5402773/SRX5402773.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX5402773/SRX5402773.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX5402773/SRX5402773.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:55:10: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:55:10: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:55:14: 7000000 INFO @ Tue, 16 Jun 2020 09:55:15: 1000000 INFO @ Tue, 16 Jun 2020 09:55:19: 8000000 INFO @ Tue, 16 Jun 2020 09:55:20: 2000000 INFO @ Tue, 16 Jun 2020 09:55:25: 9000000 INFO @ Tue, 16 Jun 2020 09:55:25: 3000000 INFO @ Tue, 16 Jun 2020 09:55:30: 10000000 INFO @ Tue, 16 Jun 2020 09:55:30: 4000000 INFO @ Tue, 16 Jun 2020 09:55:35: 11000000 INFO @ Tue, 16 Jun 2020 09:55:36: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:55:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX5402773/SRX5402773.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX5402773/SRX5402773.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX5402773/SRX5402773.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX5402773/SRX5402773.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:55:40: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:55:40: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:55:40: 12000000 INFO @ Tue, 16 Jun 2020 09:55:41: 6000000 INFO @ Tue, 16 Jun 2020 09:55:46: 1000000 INFO @ Tue, 16 Jun 2020 09:55:46: 13000000 INFO @ Tue, 16 Jun 2020 09:55:47: 7000000 INFO @ Tue, 16 Jun 2020 09:55:51: 14000000 INFO @ Tue, 16 Jun 2020 09:55:52: 8000000 INFO @ Tue, 16 Jun 2020 09:55:52: 2000000 INFO @ Tue, 16 Jun 2020 09:55:57: 15000000 INFO @ Tue, 16 Jun 2020 09:55:58: 9000000 INFO @ Tue, 16 Jun 2020 09:55:58: 3000000 INFO @ Tue, 16 Jun 2020 09:56:02: 16000000 INFO @ Tue, 16 Jun 2020 09:56:03: 10000000 INFO @ Tue, 16 Jun 2020 09:56:05: 4000000 INFO @ Tue, 16 Jun 2020 09:56:08: 17000000 INFO @ Tue, 16 Jun 2020 09:56:09: 11000000 INFO @ Tue, 16 Jun 2020 09:56:11: 5000000 INFO @ Tue, 16 Jun 2020 09:56:13: 18000000 INFO @ Tue, 16 Jun 2020 09:56:14: 12000000 INFO @ Tue, 16 Jun 2020 09:56:17: 6000000 INFO @ Tue, 16 Jun 2020 09:56:19: 19000000 INFO @ Tue, 16 Jun 2020 09:56:20: 13000000 INFO @ Tue, 16 Jun 2020 09:56:24: 7000000 INFO @ Tue, 16 Jun 2020 09:56:24: 20000000 INFO @ Tue, 16 Jun 2020 09:56:26: 14000000 INFO @ Tue, 16 Jun 2020 09:56:29: 21000000 INFO @ Tue, 16 Jun 2020 09:56:30: 8000000 INFO @ Tue, 16 Jun 2020 09:56:31: 15000000 INFO @ Tue, 16 Jun 2020 09:56:35: 22000000 INFO @ Tue, 16 Jun 2020 09:56:37: 9000000 INFO @ Tue, 16 Jun 2020 09:56:37: 16000000 INFO @ Tue, 16 Jun 2020 09:56:41: 23000000 INFO @ Tue, 16 Jun 2020 09:56:42: 17000000 INFO @ Tue, 16 Jun 2020 09:56:43: 10000000 INFO @ Tue, 16 Jun 2020 09:56:46: 24000000 INFO @ Tue, 16 Jun 2020 09:56:48: 18000000 INFO @ Tue, 16 Jun 2020 09:56:49: 11000000 INFO @ Tue, 16 Jun 2020 09:56:52: 25000000 INFO @ Tue, 16 Jun 2020 09:56:54: 19000000 INFO @ Tue, 16 Jun 2020 09:56:55: 12000000 INFO @ Tue, 16 Jun 2020 09:56:57: 26000000 INFO @ Tue, 16 Jun 2020 09:56:59: 20000000 INFO @ Tue, 16 Jun 2020 09:57:02: 13000000 INFO @ Tue, 16 Jun 2020 09:57:03: 27000000 INFO @ Tue, 16 Jun 2020 09:57:05: 21000000 INFO @ Tue, 16 Jun 2020 09:57:07: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:57:07: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:57:07: #1 total tags in treatment: 27725962 INFO @ Tue, 16 Jun 2020 09:57:07: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:57:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:57:08: #1 tags after filtering in treatment: 27725962 INFO @ Tue, 16 Jun 2020 09:57:08: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:57:08: #1 finished! INFO @ Tue, 16 Jun 2020 09:57:08: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:57:08: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:57:08: 14000000 INFO @ Tue, 16 Jun 2020 09:57:09: #2 number of paired peaks: 87 WARNING @ Tue, 16 Jun 2020 09:57:09: Too few paired peaks (87) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:57:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX5402773/SRX5402773.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5402773/SRX5402773.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5402773/SRX5402773.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5402773/SRX5402773.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:57:11: 22000000 INFO @ Tue, 16 Jun 2020 09:57:14: 15000000 INFO @ Tue, 16 Jun 2020 09:57:16: 23000000 INFO @ Tue, 16 Jun 2020 09:57:20: 16000000 INFO @ Tue, 16 Jun 2020 09:57:22: 24000000 INFO @ Tue, 16 Jun 2020 09:57:26: 17000000 INFO @ Tue, 16 Jun 2020 09:57:28: 25000000 INFO @ Tue, 16 Jun 2020 09:57:32: 18000000 INFO @ Tue, 16 Jun 2020 09:57:33: 26000000 INFO @ Tue, 16 Jun 2020 09:57:39: 19000000 INFO @ Tue, 16 Jun 2020 09:57:39: 27000000 INFO @ Tue, 16 Jun 2020 09:57:43: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:57:43: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:57:43: #1 total tags in treatment: 27725962 INFO @ Tue, 16 Jun 2020 09:57:43: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:57:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:57:44: #1 tags after filtering in treatment: 27725962 INFO @ Tue, 16 Jun 2020 09:57:44: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:57:44: #1 finished! INFO @ Tue, 16 Jun 2020 09:57:44: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:57:44: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:57:45: 20000000 INFO @ Tue, 16 Jun 2020 09:57:46: #2 number of paired peaks: 87 WARNING @ Tue, 16 Jun 2020 09:57:46: Too few paired peaks (87) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:57:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX5402773/SRX5402773.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5402773/SRX5402773.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5402773/SRX5402773.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5402773/SRX5402773.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 09:57:51: 21000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:57:57: 22000000 INFO @ Tue, 16 Jun 2020 09:58:03: 23000000 INFO @ Tue, 16 Jun 2020 09:58:09: 24000000 INFO @ Tue, 16 Jun 2020 09:58:15: 25000000 INFO @ Tue, 16 Jun 2020 09:58:21: 26000000 INFO @ Tue, 16 Jun 2020 09:58:27: 27000000 INFO @ Tue, 16 Jun 2020 09:58:32: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 09:58:32: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 09:58:32: #1 total tags in treatment: 27725962 INFO @ Tue, 16 Jun 2020 09:58:32: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:58:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:58:32: #1 tags after filtering in treatment: 27725962 INFO @ Tue, 16 Jun 2020 09:58:32: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:58:32: #1 finished! INFO @ Tue, 16 Jun 2020 09:58:32: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:58:32: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:58:34: #2 number of paired peaks: 87 WARNING @ Tue, 16 Jun 2020 09:58:34: Too few paired peaks (87) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 09:58:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX5402773/SRX5402773.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 4 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5402773/SRX5402773.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5402773/SRX5402773.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5402773/SRX5402773.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。