Job ID = 6368734 SRX = SRX5402758 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-16T00:38:01 prefetch.2.10.7: 1) Downloading 'SRR8602992'... 2020-06-16T00:38:01 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:43:41 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:43:41 prefetch.2.10.7: 1) 'SRR8602992' was downloaded successfully Read 45786124 spots for SRR8602992/SRR8602992.sra Written 45786124 spots for SRR8602992/SRR8602992.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:33 45786124 reads; of these: 45786124 (100.00%) were unpaired; of these: 6628678 (14.48%) aligned 0 times 32103216 (70.12%) aligned exactly 1 time 7054230 (15.41%) aligned >1 times 85.52% overall alignment rate Time searching: 00:09:33 Overall time: 00:09:33 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 6672825 / 39157446 = 0.1704 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:07:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX5402758/SRX5402758.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX5402758/SRX5402758.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX5402758/SRX5402758.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX5402758/SRX5402758.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:07:36: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:07:36: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:07:43: 1000000 INFO @ Tue, 16 Jun 2020 10:07:49: 2000000 INFO @ Tue, 16 Jun 2020 10:07:54: 3000000 INFO @ Tue, 16 Jun 2020 10:08:00: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:08:05: 5000000 INFO @ Tue, 16 Jun 2020 10:08:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX5402758/SRX5402758.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX5402758/SRX5402758.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX5402758/SRX5402758.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX5402758/SRX5402758.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:08:06: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:08:06: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:08:11: 6000000 INFO @ Tue, 16 Jun 2020 10:08:12: 1000000 INFO @ Tue, 16 Jun 2020 10:08:16: 7000000 INFO @ Tue, 16 Jun 2020 10:08:18: 2000000 INFO @ Tue, 16 Jun 2020 10:08:22: 8000000 INFO @ Tue, 16 Jun 2020 10:08:24: 3000000 INFO @ Tue, 16 Jun 2020 10:08:28: 9000000 INFO @ Tue, 16 Jun 2020 10:08:29: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:08:34: 10000000 INFO @ Tue, 16 Jun 2020 10:08:35: 5000000 INFO @ Tue, 16 Jun 2020 10:08:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX5402758/SRX5402758.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX5402758/SRX5402758.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX5402758/SRX5402758.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX5402758/SRX5402758.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:08:36: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:08:36: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:08:41: 11000000 INFO @ Tue, 16 Jun 2020 10:08:41: 6000000 INFO @ Tue, 16 Jun 2020 10:08:43: 1000000 INFO @ Tue, 16 Jun 2020 10:08:47: 12000000 INFO @ Tue, 16 Jun 2020 10:08:47: 7000000 INFO @ Tue, 16 Jun 2020 10:08:49: 2000000 INFO @ Tue, 16 Jun 2020 10:08:53: 13000000 INFO @ Tue, 16 Jun 2020 10:08:53: 8000000 INFO @ Tue, 16 Jun 2020 10:08:55: 3000000 INFO @ Tue, 16 Jun 2020 10:08:59: 14000000 INFO @ Tue, 16 Jun 2020 10:09:00: 9000000 INFO @ Tue, 16 Jun 2020 10:09:01: 4000000 INFO @ Tue, 16 Jun 2020 10:09:06: 10000000 INFO @ Tue, 16 Jun 2020 10:09:06: 15000000 INFO @ Tue, 16 Jun 2020 10:09:07: 5000000 INFO @ Tue, 16 Jun 2020 10:09:12: 11000000 INFO @ Tue, 16 Jun 2020 10:09:12: 16000000 INFO @ Tue, 16 Jun 2020 10:09:13: 6000000 INFO @ Tue, 16 Jun 2020 10:09:18: 12000000 INFO @ Tue, 16 Jun 2020 10:09:19: 17000000 INFO @ Tue, 16 Jun 2020 10:09:20: 7000000 INFO @ Tue, 16 Jun 2020 10:09:24: 13000000 INFO @ Tue, 16 Jun 2020 10:09:25: 18000000 INFO @ Tue, 16 Jun 2020 10:09:26: 8000000 INFO @ Tue, 16 Jun 2020 10:09:31: 14000000 INFO @ Tue, 16 Jun 2020 10:09:31: 19000000 INFO @ Tue, 16 Jun 2020 10:09:32: 9000000 INFO @ Tue, 16 Jun 2020 10:09:37: 15000000 INFO @ Tue, 16 Jun 2020 10:09:38: 20000000 INFO @ Tue, 16 Jun 2020 10:09:39: 10000000 INFO @ Tue, 16 Jun 2020 10:09:43: 16000000 INFO @ Tue, 16 Jun 2020 10:09:44: 21000000 INFO @ Tue, 16 Jun 2020 10:09:45: 11000000 INFO @ Tue, 16 Jun 2020 10:09:49: 17000000 INFO @ Tue, 16 Jun 2020 10:09:51: 22000000 INFO @ Tue, 16 Jun 2020 10:09:51: 12000000 INFO @ Tue, 16 Jun 2020 10:09:56: 18000000 INFO @ Tue, 16 Jun 2020 10:09:57: 23000000 INFO @ Tue, 16 Jun 2020 10:09:57: 13000000 INFO @ Tue, 16 Jun 2020 10:10:02: 19000000 INFO @ Tue, 16 Jun 2020 10:10:03: 24000000 INFO @ Tue, 16 Jun 2020 10:10:04: 14000000 INFO @ Tue, 16 Jun 2020 10:10:08: 20000000 INFO @ Tue, 16 Jun 2020 10:10:10: 25000000 INFO @ Tue, 16 Jun 2020 10:10:10: 15000000 INFO @ Tue, 16 Jun 2020 10:10:15: 21000000 INFO @ Tue, 16 Jun 2020 10:10:16: 26000000 INFO @ Tue, 16 Jun 2020 10:10:17: 16000000 INFO @ Tue, 16 Jun 2020 10:10:21: 22000000 INFO @ Tue, 16 Jun 2020 10:10:23: 17000000 INFO @ Tue, 16 Jun 2020 10:10:23: 27000000 INFO @ Tue, 16 Jun 2020 10:10:28: 23000000 INFO @ Tue, 16 Jun 2020 10:10:30: 18000000 INFO @ Tue, 16 Jun 2020 10:10:30: 28000000 INFO @ Tue, 16 Jun 2020 10:10:34: 24000000 INFO @ Tue, 16 Jun 2020 10:10:36: 19000000 INFO @ Tue, 16 Jun 2020 10:10:36: 29000000 INFO @ Tue, 16 Jun 2020 10:10:41: 25000000 INFO @ Tue, 16 Jun 2020 10:10:43: 30000000 INFO @ Tue, 16 Jun 2020 10:10:43: 20000000 INFO @ Tue, 16 Jun 2020 10:10:47: 26000000 INFO @ Tue, 16 Jun 2020 10:10:49: 31000000 INFO @ Tue, 16 Jun 2020 10:10:49: 21000000 INFO @ Tue, 16 Jun 2020 10:10:54: 27000000 INFO @ Tue, 16 Jun 2020 10:10:56: 32000000 INFO @ Tue, 16 Jun 2020 10:10:56: 22000000 INFO @ Tue, 16 Jun 2020 10:11:00: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 10:11:00: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 10:11:00: #1 total tags in treatment: 32484621 INFO @ Tue, 16 Jun 2020 10:11:00: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:11:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:11:00: 28000000 INFO @ Tue, 16 Jun 2020 10:11:00: #1 tags after filtering in treatment: 32484621 INFO @ Tue, 16 Jun 2020 10:11:00: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:11:00: #1 finished! INFO @ Tue, 16 Jun 2020 10:11:00: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:11:00: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:11:02: #2 number of paired peaks: 50 WARNING @ Tue, 16 Jun 2020 10:11:02: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:11:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX5402758/SRX5402758.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5402758/SRX5402758.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5402758/SRX5402758.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5402758/SRX5402758.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:11:02: 23000000 INFO @ Tue, 16 Jun 2020 10:11:06: 29000000 INFO @ Tue, 16 Jun 2020 10:11:09: 24000000 INFO @ Tue, 16 Jun 2020 10:11:12: 30000000 INFO @ Tue, 16 Jun 2020 10:11:15: 25000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 10:11:19: 31000000 INFO @ Tue, 16 Jun 2020 10:11:21: 26000000 INFO @ Tue, 16 Jun 2020 10:11:25: 32000000 INFO @ Tue, 16 Jun 2020 10:11:27: 27000000 INFO @ Tue, 16 Jun 2020 10:11:29: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 10:11:29: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 10:11:29: #1 total tags in treatment: 32484621 INFO @ Tue, 16 Jun 2020 10:11:29: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:11:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:11:29: #1 tags after filtering in treatment: 32484621 INFO @ Tue, 16 Jun 2020 10:11:29: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:11:29: #1 finished! INFO @ Tue, 16 Jun 2020 10:11:29: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:11:29: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:11:31: #2 number of paired peaks: 50 WARNING @ Tue, 16 Jun 2020 10:11:31: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:11:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX5402758/SRX5402758.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5402758/SRX5402758.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5402758/SRX5402758.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5402758/SRX5402758.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:11:33: 28000000 INFO @ Tue, 16 Jun 2020 10:11:39: 29000000 INFO @ Tue, 16 Jun 2020 10:11:45: 30000000 INFO @ Tue, 16 Jun 2020 10:11:51: 31000000 INFO @ Tue, 16 Jun 2020 10:11:57: 32000000 INFO @ Tue, 16 Jun 2020 10:12:01: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 10:12:01: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 10:12:01: #1 total tags in treatment: 32484621 INFO @ Tue, 16 Jun 2020 10:12:01: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:12:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:12:01: #1 tags after filtering in treatment: 32484621 INFO @ Tue, 16 Jun 2020 10:12:01: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 10:12:01: #1 finished! INFO @ Tue, 16 Jun 2020 10:12:01: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:12:01: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:12:03: #2 number of paired peaks: 50 WARNING @ Tue, 16 Jun 2020 10:12:03: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 10:12:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX5402758/SRX5402758.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5402758/SRX5402758.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5402758/SRX5402758.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX5402758/SRX5402758.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。