Job ID = 6507972 SRX = SRX514728 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-26T13:07:06 prefetch.2.10.7: 1) Downloading 'SRR1231449'... 2020-06-26T13:07:06 prefetch.2.10.7: Downloading via HTTPS... 2020-06-26T13:14:54 prefetch.2.10.7: HTTPS download succeed 2020-06-26T13:14:54 prefetch.2.10.7: 1) 'SRR1231449' was downloaded successfully Read 37371616 spots for SRR1231449/SRR1231449.sra Written 37371616 spots for SRR1231449/SRR1231449.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:54 37371616 reads; of these: 37371616 (100.00%) were unpaired; of these: 193918 (0.52%) aligned 0 times 30718037 (82.20%) aligned exactly 1 time 6459661 (17.28%) aligned >1 times 99.48% overall alignment rate Time searching: 00:06:54 Overall time: 00:06:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 7091128 / 37177698 = 0.1907 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 22:30:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX514728/SRX514728.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX514728/SRX514728.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX514728/SRX514728.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX514728/SRX514728.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 22:30:15: #1 read tag files... INFO @ Fri, 26 Jun 2020 22:30:15: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 22:30:21: 1000000 INFO @ Fri, 26 Jun 2020 22:30:27: 2000000 INFO @ Fri, 26 Jun 2020 22:30:32: 3000000 INFO @ Fri, 26 Jun 2020 22:30:38: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 22:30:43: 5000000 INFO @ Fri, 26 Jun 2020 22:30:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX514728/SRX514728.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX514728/SRX514728.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX514728/SRX514728.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX514728/SRX514728.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 22:30:45: #1 read tag files... INFO @ Fri, 26 Jun 2020 22:30:45: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 22:30:49: 6000000 INFO @ Fri, 26 Jun 2020 22:30:52: 1000000 INFO @ Fri, 26 Jun 2020 22:30:55: 7000000 INFO @ Fri, 26 Jun 2020 22:30:58: 2000000 INFO @ Fri, 26 Jun 2020 22:31:02: 8000000 INFO @ Fri, 26 Jun 2020 22:31:05: 3000000 INFO @ Fri, 26 Jun 2020 22:31:08: 9000000 INFO @ Fri, 26 Jun 2020 22:31:12: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 26 Jun 2020 22:31:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX514728/SRX514728.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX514728/SRX514728.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX514728/SRX514728.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX514728/SRX514728.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 26 Jun 2020 22:31:15: #1 read tag files... INFO @ Fri, 26 Jun 2020 22:31:15: #1 read treatment tags... INFO @ Fri, 26 Jun 2020 22:31:15: 10000000 INFO @ Fri, 26 Jun 2020 22:31:19: 5000000 INFO @ Fri, 26 Jun 2020 22:31:21: 11000000 INFO @ Fri, 26 Jun 2020 22:31:22: 1000000 INFO @ Fri, 26 Jun 2020 22:31:25: 6000000 INFO @ Fri, 26 Jun 2020 22:31:28: 12000000 INFO @ Fri, 26 Jun 2020 22:31:29: 2000000 INFO @ Fri, 26 Jun 2020 22:31:32: 7000000 INFO @ Fri, 26 Jun 2020 22:31:35: 13000000 INFO @ Fri, 26 Jun 2020 22:31:36: 3000000 INFO @ Fri, 26 Jun 2020 22:31:39: 8000000 INFO @ Fri, 26 Jun 2020 22:31:41: 14000000 INFO @ Fri, 26 Jun 2020 22:31:43: 4000000 INFO @ Fri, 26 Jun 2020 22:31:47: 9000000 INFO @ Fri, 26 Jun 2020 22:31:48: 15000000 INFO @ Fri, 26 Jun 2020 22:31:50: 5000000 INFO @ Fri, 26 Jun 2020 22:31:53: 10000000 INFO @ Fri, 26 Jun 2020 22:31:54: 16000000 INFO @ Fri, 26 Jun 2020 22:31:57: 6000000 INFO @ Fri, 26 Jun 2020 22:32:01: 11000000 INFO @ Fri, 26 Jun 2020 22:32:01: 17000000 INFO @ Fri, 26 Jun 2020 22:32:04: 7000000 INFO @ Fri, 26 Jun 2020 22:32:07: 18000000 INFO @ Fri, 26 Jun 2020 22:32:08: 12000000 INFO @ Fri, 26 Jun 2020 22:32:11: 8000000 INFO @ Fri, 26 Jun 2020 22:32:14: 19000000 INFO @ Fri, 26 Jun 2020 22:32:15: 13000000 INFO @ Fri, 26 Jun 2020 22:32:18: 9000000 INFO @ Fri, 26 Jun 2020 22:32:21: 20000000 INFO @ Fri, 26 Jun 2020 22:32:22: 14000000 INFO @ Fri, 26 Jun 2020 22:32:25: 10000000 INFO @ Fri, 26 Jun 2020 22:32:27: 21000000 INFO @ Fri, 26 Jun 2020 22:32:29: 15000000 INFO @ Fri, 26 Jun 2020 22:32:32: 11000000 INFO @ Fri, 26 Jun 2020 22:32:34: 22000000 INFO @ Fri, 26 Jun 2020 22:32:36: 16000000 INFO @ Fri, 26 Jun 2020 22:32:39: 12000000 INFO @ Fri, 26 Jun 2020 22:32:40: 23000000 INFO @ Fri, 26 Jun 2020 22:32:43: 17000000 INFO @ Fri, 26 Jun 2020 22:32:47: 13000000 INFO @ Fri, 26 Jun 2020 22:32:47: 24000000 INFO @ Fri, 26 Jun 2020 22:32:50: 18000000 INFO @ Fri, 26 Jun 2020 22:32:54: 25000000 INFO @ Fri, 26 Jun 2020 22:32:54: 14000000 INFO @ Fri, 26 Jun 2020 22:32:57: 19000000 INFO @ Fri, 26 Jun 2020 22:33:00: 26000000 INFO @ Fri, 26 Jun 2020 22:33:01: 15000000 INFO @ Fri, 26 Jun 2020 22:33:04: 20000000 INFO @ Fri, 26 Jun 2020 22:33:06: 27000000 INFO @ Fri, 26 Jun 2020 22:33:08: 16000000 INFO @ Fri, 26 Jun 2020 22:33:11: 21000000 INFO @ Fri, 26 Jun 2020 22:33:13: 28000000 INFO @ Fri, 26 Jun 2020 22:33:15: 17000000 INFO @ Fri, 26 Jun 2020 22:33:18: 22000000 INFO @ Fri, 26 Jun 2020 22:33:20: 29000000 INFO @ Fri, 26 Jun 2020 22:33:22: 18000000 INFO @ Fri, 26 Jun 2020 22:33:25: 23000000 INFO @ Fri, 26 Jun 2020 22:33:26: 30000000 INFO @ Fri, 26 Jun 2020 22:33:27: #1 tag size is determined as 42 bps INFO @ Fri, 26 Jun 2020 22:33:27: #1 tag size = 42 INFO @ Fri, 26 Jun 2020 22:33:27: #1 total tags in treatment: 30086570 INFO @ Fri, 26 Jun 2020 22:33:27: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 22:33:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 22:33:28: #1 tags after filtering in treatment: 30086570 INFO @ Fri, 26 Jun 2020 22:33:28: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 22:33:28: #1 finished! INFO @ Fri, 26 Jun 2020 22:33:28: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 22:33:28: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 22:33:30: 19000000 INFO @ Fri, 26 Jun 2020 22:33:30: #2 number of paired peaks: 82 WARNING @ Fri, 26 Jun 2020 22:33:30: Too few paired peaks (82) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 22:33:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX514728/SRX514728.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX514728/SRX514728.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX514728/SRX514728.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX514728/SRX514728.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 26 Jun 2020 22:33:33: 24000000 INFO @ Fri, 26 Jun 2020 22:33:37: 20000000 INFO @ Fri, 26 Jun 2020 22:33:40: 25000000 INFO @ Fri, 26 Jun 2020 22:33:44: 21000000 INFO @ Fri, 26 Jun 2020 22:33:47: 26000000 INFO @ Fri, 26 Jun 2020 22:33:51: 22000000 INFO @ Fri, 26 Jun 2020 22:33:53: 27000000 INFO @ Fri, 26 Jun 2020 22:33:58: 23000000 INFO @ Fri, 26 Jun 2020 22:34:01: 28000000 INFO @ Fri, 26 Jun 2020 22:34:05: 24000000 INFO @ Fri, 26 Jun 2020 22:34:08: 29000000 INFO @ Fri, 26 Jun 2020 22:34:12: 25000000 INFO @ Fri, 26 Jun 2020 22:34:15: 30000000 INFO @ Fri, 26 Jun 2020 22:34:16: #1 tag size is determined as 42 bps INFO @ Fri, 26 Jun 2020 22:34:16: #1 tag size = 42 INFO @ Fri, 26 Jun 2020 22:34:16: #1 total tags in treatment: 30086570 INFO @ Fri, 26 Jun 2020 22:34:16: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 22:34:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 22:34:16: #1 tags after filtering in treatment: 30086570 INFO @ Fri, 26 Jun 2020 22:34:16: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 22:34:16: #1 finished! INFO @ Fri, 26 Jun 2020 22:34:16: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 22:34:16: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 22:34:18: #2 number of paired peaks: 82 WARNING @ Fri, 26 Jun 2020 22:34:18: Too few paired peaks (82) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 22:34:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX514728/SRX514728.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX514728/SRX514728.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX514728/SRX514728.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX514728/SRX514728.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 26 Jun 2020 22:34:19: 26000000 INFO @ Fri, 26 Jun 2020 22:34:25: 27000000 INFO @ Fri, 26 Jun 2020 22:34:32: 28000000 BigWig に変換しました。 INFO @ Fri, 26 Jun 2020 22:34:38: 29000000 INFO @ Fri, 26 Jun 2020 22:34:45: 30000000 INFO @ Fri, 26 Jun 2020 22:34:45: #1 tag size is determined as 42 bps INFO @ Fri, 26 Jun 2020 22:34:45: #1 tag size = 42 INFO @ Fri, 26 Jun 2020 22:34:45: #1 total tags in treatment: 30086570 INFO @ Fri, 26 Jun 2020 22:34:45: #1 user defined the maximum tags... INFO @ Fri, 26 Jun 2020 22:34:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 26 Jun 2020 22:34:46: #1 tags after filtering in treatment: 30086570 INFO @ Fri, 26 Jun 2020 22:34:46: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 26 Jun 2020 22:34:46: #1 finished! INFO @ Fri, 26 Jun 2020 22:34:46: #2 Build Peak Model... INFO @ Fri, 26 Jun 2020 22:34:46: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 26 Jun 2020 22:34:48: #2 number of paired peaks: 82 WARNING @ Fri, 26 Jun 2020 22:34:48: Too few paired peaks (82) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 26 Jun 2020 22:34:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX514728/SRX514728.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX514728/SRX514728.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX514728/SRX514728.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX514728/SRX514728.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling