Job ID = 6368459 SRX = SRX4996816 Genome = ce11 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-06-16T00:16:21 prefetch.2.10.7: 1) Downloading 'SRR8176701'... 2020-06-16T00:16:21 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:23:31 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:23:31 prefetch.2.10.7: 1) 'SRR8176701' was downloaded successfully 2020-06-16T00:23:31 prefetch.2.10.7: 'SRR8176701' has 0 unresolved dependencies Read 32287963 spots for SRR8176701/SRR8176701.sra Written 32287963 spots for SRR8176701/SRR8176701.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:28:32 32287963 reads; of these: 32287963 (100.00%) were paired; of these: 1773681 (5.49%) aligned concordantly 0 times 26028291 (80.61%) aligned concordantly exactly 1 time 4485991 (13.89%) aligned concordantly >1 times ---- 1773681 pairs aligned concordantly 0 times; of these: 1053439 (59.39%) aligned discordantly 1 time ---- 720242 pairs aligned 0 times concordantly or discordantly; of these: 1440484 mates make up the pairs; of these: 727040 (50.47%) aligned 0 times 421536 (29.26%) aligned exactly 1 time 291908 (20.26%) aligned >1 times 98.87% overall alignment rate Time searching: 00:28:32 Overall time: 00:28:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 28 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 587886 / 31546009 = 0.0186 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:10:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX4996816/SRX4996816.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX4996816/SRX4996816.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX4996816/SRX4996816.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX4996816/SRX4996816.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:10:10: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:10:10: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:10:15: 1000000 INFO @ Tue, 16 Jun 2020 10:10:19: 2000000 INFO @ Tue, 16 Jun 2020 10:10:24: 3000000 INFO @ Tue, 16 Jun 2020 10:10:29: 4000000 INFO @ Tue, 16 Jun 2020 10:10:33: 5000000 INFO @ Tue, 16 Jun 2020 10:10:38: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:10:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX4996816/SRX4996816.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX4996816/SRX4996816.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX4996816/SRX4996816.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX4996816/SRX4996816.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:10:40: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:10:40: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:10:42: 7000000 INFO @ Tue, 16 Jun 2020 10:10:45: 1000000 INFO @ Tue, 16 Jun 2020 10:10:47: 8000000 INFO @ Tue, 16 Jun 2020 10:10:50: 2000000 INFO @ Tue, 16 Jun 2020 10:10:52: 9000000 INFO @ Tue, 16 Jun 2020 10:10:55: 3000000 INFO @ Tue, 16 Jun 2020 10:10:58: 10000000 INFO @ Tue, 16 Jun 2020 10:11:00: 4000000 INFO @ Tue, 16 Jun 2020 10:11:03: 11000000 INFO @ Tue, 16 Jun 2020 10:11:05: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 10:11:08: 12000000 INFO @ Tue, 16 Jun 2020 10:11:10: 6000000 INFO @ Tue, 16 Jun 2020 10:11:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX4996816/SRX4996816.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX4996816/SRX4996816.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX4996816/SRX4996816.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX4996816/SRX4996816.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 10:11:10: #1 read tag files... INFO @ Tue, 16 Jun 2020 10:11:10: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 10:11:13: 13000000 INFO @ Tue, 16 Jun 2020 10:11:15: 1000000 INFO @ Tue, 16 Jun 2020 10:11:15: 7000000 INFO @ Tue, 16 Jun 2020 10:11:18: 14000000 INFO @ Tue, 16 Jun 2020 10:11:20: 2000000 INFO @ Tue, 16 Jun 2020 10:11:20: 8000000 INFO @ Tue, 16 Jun 2020 10:11:23: 15000000 INFO @ Tue, 16 Jun 2020 10:11:25: 3000000 INFO @ Tue, 16 Jun 2020 10:11:26: 9000000 INFO @ Tue, 16 Jun 2020 10:11:28: 16000000 INFO @ Tue, 16 Jun 2020 10:11:30: 4000000 INFO @ Tue, 16 Jun 2020 10:11:31: 10000000 INFO @ Tue, 16 Jun 2020 10:11:34: 17000000 INFO @ Tue, 16 Jun 2020 10:11:35: 5000000 INFO @ Tue, 16 Jun 2020 10:11:36: 11000000 INFO @ Tue, 16 Jun 2020 10:11:39: 18000000 INFO @ Tue, 16 Jun 2020 10:11:40: 6000000 INFO @ Tue, 16 Jun 2020 10:11:41: 12000000 INFO @ Tue, 16 Jun 2020 10:11:44: 19000000 INFO @ Tue, 16 Jun 2020 10:11:46: 7000000 INFO @ Tue, 16 Jun 2020 10:11:46: 13000000 INFO @ Tue, 16 Jun 2020 10:11:49: 20000000 INFO @ Tue, 16 Jun 2020 10:11:51: 8000000 INFO @ Tue, 16 Jun 2020 10:11:52: 14000000 INFO @ Tue, 16 Jun 2020 10:11:54: 21000000 INFO @ Tue, 16 Jun 2020 10:11:56: 9000000 INFO @ Tue, 16 Jun 2020 10:11:57: 15000000 INFO @ Tue, 16 Jun 2020 10:11:59: 22000000 INFO @ Tue, 16 Jun 2020 10:12:01: 10000000 INFO @ Tue, 16 Jun 2020 10:12:02: 16000000 INFO @ Tue, 16 Jun 2020 10:12:04: 23000000 INFO @ Tue, 16 Jun 2020 10:12:07: 11000000 INFO @ Tue, 16 Jun 2020 10:12:08: 17000000 INFO @ Tue, 16 Jun 2020 10:12:10: 24000000 INFO @ Tue, 16 Jun 2020 10:12:13: 12000000 INFO @ Tue, 16 Jun 2020 10:12:13: 18000000 INFO @ Tue, 16 Jun 2020 10:12:15: 25000000 INFO @ Tue, 16 Jun 2020 10:12:19: 13000000 INFO @ Tue, 16 Jun 2020 10:12:19: 19000000 INFO @ Tue, 16 Jun 2020 10:12:20: 26000000 INFO @ Tue, 16 Jun 2020 10:12:24: 20000000 INFO @ Tue, 16 Jun 2020 10:12:24: 14000000 INFO @ Tue, 16 Jun 2020 10:12:26: 27000000 INFO @ Tue, 16 Jun 2020 10:12:30: 21000000 INFO @ Tue, 16 Jun 2020 10:12:30: 15000000 INFO @ Tue, 16 Jun 2020 10:12:31: 28000000 INFO @ Tue, 16 Jun 2020 10:12:35: 22000000 INFO @ Tue, 16 Jun 2020 10:12:36: 16000000 INFO @ Tue, 16 Jun 2020 10:12:36: 29000000 INFO @ Tue, 16 Jun 2020 10:12:40: 23000000 INFO @ Tue, 16 Jun 2020 10:12:41: 17000000 INFO @ Tue, 16 Jun 2020 10:12:41: 30000000 INFO @ Tue, 16 Jun 2020 10:12:46: 24000000 INFO @ Tue, 16 Jun 2020 10:12:47: 18000000 INFO @ Tue, 16 Jun 2020 10:12:47: 31000000 INFO @ Tue, 16 Jun 2020 10:12:51: 25000000 INFO @ Tue, 16 Jun 2020 10:12:52: 32000000 INFO @ Tue, 16 Jun 2020 10:12:52: 19000000 INFO @ Tue, 16 Jun 2020 10:12:57: 26000000 INFO @ Tue, 16 Jun 2020 10:12:57: 33000000 INFO @ Tue, 16 Jun 2020 10:12:58: 20000000 INFO @ Tue, 16 Jun 2020 10:13:02: 27000000 INFO @ Tue, 16 Jun 2020 10:13:03: 34000000 INFO @ Tue, 16 Jun 2020 10:13:03: 21000000 INFO @ Tue, 16 Jun 2020 10:13:07: 28000000 INFO @ Tue, 16 Jun 2020 10:13:08: 35000000 INFO @ Tue, 16 Jun 2020 10:13:09: 22000000 INFO @ Tue, 16 Jun 2020 10:13:13: 29000000 INFO @ Tue, 16 Jun 2020 10:13:13: 36000000 INFO @ Tue, 16 Jun 2020 10:13:14: 23000000 INFO @ Tue, 16 Jun 2020 10:13:18: 30000000 INFO @ Tue, 16 Jun 2020 10:13:19: 37000000 INFO @ Tue, 16 Jun 2020 10:13:20: 24000000 INFO @ Tue, 16 Jun 2020 10:13:23: 31000000 INFO @ Tue, 16 Jun 2020 10:13:25: 38000000 INFO @ Tue, 16 Jun 2020 10:13:25: 25000000 INFO @ Tue, 16 Jun 2020 10:13:29: 32000000 INFO @ Tue, 16 Jun 2020 10:13:30: 39000000 INFO @ Tue, 16 Jun 2020 10:13:31: 26000000 INFO @ Tue, 16 Jun 2020 10:13:34: 33000000 INFO @ Tue, 16 Jun 2020 10:13:36: 40000000 INFO @ Tue, 16 Jun 2020 10:13:37: 27000000 INFO @ Tue, 16 Jun 2020 10:13:39: 34000000 INFO @ Tue, 16 Jun 2020 10:13:41: 41000000 INFO @ Tue, 16 Jun 2020 10:13:42: 28000000 INFO @ Tue, 16 Jun 2020 10:13:45: 35000000 INFO @ Tue, 16 Jun 2020 10:13:47: 42000000 INFO @ Tue, 16 Jun 2020 10:13:47: 29000000 INFO @ Tue, 16 Jun 2020 10:13:50: 36000000 INFO @ Tue, 16 Jun 2020 10:13:53: 30000000 INFO @ Tue, 16 Jun 2020 10:13:53: 43000000 INFO @ Tue, 16 Jun 2020 10:13:55: 37000000 INFO @ Tue, 16 Jun 2020 10:13:58: 31000000 INFO @ Tue, 16 Jun 2020 10:13:59: 44000000 INFO @ Tue, 16 Jun 2020 10:14:02: 38000000 INFO @ Tue, 16 Jun 2020 10:14:04: 32000000 INFO @ Tue, 16 Jun 2020 10:14:05: 45000000 INFO @ Tue, 16 Jun 2020 10:14:07: 39000000 INFO @ Tue, 16 Jun 2020 10:14:10: 33000000 INFO @ Tue, 16 Jun 2020 10:14:11: 46000000 INFO @ Tue, 16 Jun 2020 10:14:12: 40000000 INFO @ Tue, 16 Jun 2020 10:14:15: 34000000 INFO @ Tue, 16 Jun 2020 10:14:17: 47000000 INFO @ Tue, 16 Jun 2020 10:14:18: 41000000 INFO @ Tue, 16 Jun 2020 10:14:20: 35000000 INFO @ Tue, 16 Jun 2020 10:14:23: 48000000 INFO @ Tue, 16 Jun 2020 10:14:24: 42000000 INFO @ Tue, 16 Jun 2020 10:14:26: 36000000 INFO @ Tue, 16 Jun 2020 10:14:29: 49000000 INFO @ Tue, 16 Jun 2020 10:14:30: 43000000 INFO @ Tue, 16 Jun 2020 10:14:32: 37000000 INFO @ Tue, 16 Jun 2020 10:14:35: 50000000 INFO @ Tue, 16 Jun 2020 10:14:36: 44000000 INFO @ Tue, 16 Jun 2020 10:14:38: 38000000 INFO @ Tue, 16 Jun 2020 10:14:40: 51000000 INFO @ Tue, 16 Jun 2020 10:14:42: 45000000 INFO @ Tue, 16 Jun 2020 10:14:44: 39000000 INFO @ Tue, 16 Jun 2020 10:14:46: 52000000 INFO @ Tue, 16 Jun 2020 10:14:48: 46000000 INFO @ Tue, 16 Jun 2020 10:14:49: 40000000 INFO @ Tue, 16 Jun 2020 10:14:53: 53000000 INFO @ Tue, 16 Jun 2020 10:14:54: 47000000 INFO @ Tue, 16 Jun 2020 10:14:55: 41000000 INFO @ Tue, 16 Jun 2020 10:14:59: 54000000 INFO @ Tue, 16 Jun 2020 10:15:00: 48000000 INFO @ Tue, 16 Jun 2020 10:15:01: 42000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 10:15:05: 55000000 INFO @ Tue, 16 Jun 2020 10:15:06: 49000000 INFO @ Tue, 16 Jun 2020 10:15:08: 43000000 INFO @ Tue, 16 Jun 2020 10:15:11: 56000000 INFO @ Tue, 16 Jun 2020 10:15:13: 50000000 INFO @ Tue, 16 Jun 2020 10:15:14: 44000000 INFO @ Tue, 16 Jun 2020 10:15:17: 57000000 INFO @ Tue, 16 Jun 2020 10:15:19: 51000000 INFO @ Tue, 16 Jun 2020 10:15:20: 45000000 INFO @ Tue, 16 Jun 2020 10:15:23: 58000000 INFO @ Tue, 16 Jun 2020 10:15:26: 52000000 INFO @ Tue, 16 Jun 2020 10:15:27: 46000000 INFO @ Tue, 16 Jun 2020 10:15:29: 59000000 INFO @ Tue, 16 Jun 2020 10:15:32: 53000000 INFO @ Tue, 16 Jun 2020 10:15:33: 47000000 INFO @ Tue, 16 Jun 2020 10:15:35: 60000000 INFO @ Tue, 16 Jun 2020 10:15:39: 54000000 INFO @ Tue, 16 Jun 2020 10:15:40: 48000000 INFO @ Tue, 16 Jun 2020 10:15:41: 61000000 INFO @ Tue, 16 Jun 2020 10:15:46: 55000000 INFO @ Tue, 16 Jun 2020 10:15:46: 49000000 INFO @ Tue, 16 Jun 2020 10:15:47: 62000000 INFO @ Tue, 16 Jun 2020 10:15:51: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 10:15:51: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 10:15:51: #1 total tags in treatment: 29933136 INFO @ Tue, 16 Jun 2020 10:15:51: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:15:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:15:51: #1 tags after filtering in treatment: 26061949 INFO @ Tue, 16 Jun 2020 10:15:51: #1 Redundant rate of treatment: 0.13 INFO @ Tue, 16 Jun 2020 10:15:51: #1 finished! INFO @ Tue, 16 Jun 2020 10:15:51: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:15:51: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:15:52: 50000000 INFO @ Tue, 16 Jun 2020 10:15:52: 56000000 INFO @ Tue, 16 Jun 2020 10:15:53: #2 number of paired peaks: 174 WARNING @ Tue, 16 Jun 2020 10:15:53: Fewer paired peaks (174) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 174 pairs to build model! INFO @ Tue, 16 Jun 2020 10:15:53: start model_add_line... INFO @ Tue, 16 Jun 2020 10:15:53: start X-correlation... INFO @ Tue, 16 Jun 2020 10:15:53: end of X-cor INFO @ Tue, 16 Jun 2020 10:15:53: #2 finished! INFO @ Tue, 16 Jun 2020 10:15:53: #2 predicted fragment length is 51 bps INFO @ Tue, 16 Jun 2020 10:15:53: #2 alternative fragment length(s) may be 2,51,68,108 bps INFO @ Tue, 16 Jun 2020 10:15:53: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX4996816/SRX4996816.05_model.r WARNING @ Tue, 16 Jun 2020 10:15:53: #2 Since the d (51) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 10:15:53: #2 You may need to consider one of the other alternative d(s): 2,51,68,108 WARNING @ Tue, 16 Jun 2020 10:15:53: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 10:15:53: #3 Call peaks... INFO @ Tue, 16 Jun 2020 10:15:53: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 10:15:58: 51000000 INFO @ Tue, 16 Jun 2020 10:15:58: 57000000 INFO @ Tue, 16 Jun 2020 10:16:04: 52000000 INFO @ Tue, 16 Jun 2020 10:16:04: 58000000 INFO @ Tue, 16 Jun 2020 10:16:10: 59000000 INFO @ Tue, 16 Jun 2020 10:16:10: 53000000 INFO @ Tue, 16 Jun 2020 10:16:16: 60000000 INFO @ Tue, 16 Jun 2020 10:16:17: 54000000 INFO @ Tue, 16 Jun 2020 10:16:22: 61000000 INFO @ Tue, 16 Jun 2020 10:16:23: 55000000 INFO @ Tue, 16 Jun 2020 10:16:27: 62000000 INFO @ Tue, 16 Jun 2020 10:16:29: 56000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 10:16:31: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 10:16:31: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 10:16:31: #1 total tags in treatment: 29933136 INFO @ Tue, 16 Jun 2020 10:16:31: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:16:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:16:32: #1 tags after filtering in treatment: 26061949 INFO @ Tue, 16 Jun 2020 10:16:32: #1 Redundant rate of treatment: 0.13 INFO @ Tue, 16 Jun 2020 10:16:32: #1 finished! INFO @ Tue, 16 Jun 2020 10:16:32: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:16:32: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:16:34: #2 number of paired peaks: 174 WARNING @ Tue, 16 Jun 2020 10:16:34: Fewer paired peaks (174) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 174 pairs to build model! INFO @ Tue, 16 Jun 2020 10:16:34: start model_add_line... INFO @ Tue, 16 Jun 2020 10:16:34: start X-correlation... INFO @ Tue, 16 Jun 2020 10:16:34: end of X-cor INFO @ Tue, 16 Jun 2020 10:16:34: #2 finished! INFO @ Tue, 16 Jun 2020 10:16:34: #2 predicted fragment length is 51 bps INFO @ Tue, 16 Jun 2020 10:16:34: #2 alternative fragment length(s) may be 2,51,68,108 bps INFO @ Tue, 16 Jun 2020 10:16:34: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX4996816/SRX4996816.10_model.r WARNING @ Tue, 16 Jun 2020 10:16:34: #2 Since the d (51) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 10:16:34: #2 You may need to consider one of the other alternative d(s): 2,51,68,108 WARNING @ Tue, 16 Jun 2020 10:16:34: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 10:16:34: #3 Call peaks... INFO @ Tue, 16 Jun 2020 10:16:34: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 10:16:35: 57000000 INFO @ Tue, 16 Jun 2020 10:16:36: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 10:16:40: 58000000 INFO @ Tue, 16 Jun 2020 10:16:45: 59000000 INFO @ Tue, 16 Jun 2020 10:16:51: 60000000 INFO @ Tue, 16 Jun 2020 10:16:56: 61000000 INFO @ Tue, 16 Jun 2020 10:16:57: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX4996816/SRX4996816.05_peaks.xls INFO @ Tue, 16 Jun 2020 10:16:57: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX4996816/SRX4996816.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 10:16:57: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX4996816/SRX4996816.05_summits.bed INFO @ Tue, 16 Jun 2020 10:16:57: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (667 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:17:02: 62000000 INFO @ Tue, 16 Jun 2020 10:17:06: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 10:17:06: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 10:17:06: #1 total tags in treatment: 29933136 INFO @ Tue, 16 Jun 2020 10:17:06: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 10:17:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 10:17:06: #1 tags after filtering in treatment: 26061949 INFO @ Tue, 16 Jun 2020 10:17:06: #1 Redundant rate of treatment: 0.13 INFO @ Tue, 16 Jun 2020 10:17:06: #1 finished! INFO @ Tue, 16 Jun 2020 10:17:06: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 10:17:06: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 10:17:08: #2 number of paired peaks: 174 WARNING @ Tue, 16 Jun 2020 10:17:08: Fewer paired peaks (174) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 174 pairs to build model! INFO @ Tue, 16 Jun 2020 10:17:08: start model_add_line... INFO @ Tue, 16 Jun 2020 10:17:08: start X-correlation... INFO @ Tue, 16 Jun 2020 10:17:08: end of X-cor INFO @ Tue, 16 Jun 2020 10:17:08: #2 finished! INFO @ Tue, 16 Jun 2020 10:17:08: #2 predicted fragment length is 51 bps INFO @ Tue, 16 Jun 2020 10:17:08: #2 alternative fragment length(s) may be 2,51,68,108 bps INFO @ Tue, 16 Jun 2020 10:17:08: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX4996816/SRX4996816.20_model.r WARNING @ Tue, 16 Jun 2020 10:17:08: #2 Since the d (51) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 10:17:08: #2 You may need to consider one of the other alternative d(s): 2,51,68,108 WARNING @ Tue, 16 Jun 2020 10:17:08: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 10:17:08: #3 Call peaks... INFO @ Tue, 16 Jun 2020 10:17:08: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 10:17:16: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 10:17:36: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX4996816/SRX4996816.10_peaks.xls INFO @ Tue, 16 Jun 2020 10:17:36: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX4996816/SRX4996816.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 10:17:36: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX4996816/SRX4996816.10_summits.bed INFO @ Tue, 16 Jun 2020 10:17:36: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (403 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 10:17:50: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 10:18:09: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX4996816/SRX4996816.20_peaks.xls INFO @ Tue, 16 Jun 2020 10:18:09: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX4996816/SRX4996816.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 10:18:09: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX4996816/SRX4996816.20_summits.bed INFO @ Tue, 16 Jun 2020 10:18:09: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (132 records, 4 fields): 1 millis CompletedMACS2peakCalling