Job ID = 6368412 SRX = SRX495091 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-16T00:09:54 prefetch.2.10.7: 1) Downloading 'SRR1198623'... 2020-06-16T00:09:54 prefetch.2.10.7: Downloading via HTTPS... 2020-06-16T00:11:20 prefetch.2.10.7: HTTPS download succeed 2020-06-16T00:11:21 prefetch.2.10.7: 'SRR1198623' is valid 2020-06-16T00:11:21 prefetch.2.10.7: 1) 'SRR1198623' was downloaded successfully Read 6747970 spots for SRR1198623/SRR1198623.sra Written 6747970 spots for SRR1198623/SRR1198623.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:26 6747970 reads; of these: 6747970 (100.00%) were unpaired; of these: 375811 (5.57%) aligned 0 times 5167110 (76.57%) aligned exactly 1 time 1205049 (17.86%) aligned >1 times 94.43% overall alignment rate Time searching: 00:01:26 Overall time: 00:01:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 4769576 / 6372159 = 0.7485 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:14:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX495091/SRX495091.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX495091/SRX495091.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX495091/SRX495091.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX495091/SRX495091.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:14:36: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:14:36: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:14:40: 1000000 INFO @ Tue, 16 Jun 2020 09:14:43: #1 tag size is determined as 42 bps INFO @ Tue, 16 Jun 2020 09:14:43: #1 tag size = 42 INFO @ Tue, 16 Jun 2020 09:14:43: #1 total tags in treatment: 1602583 INFO @ Tue, 16 Jun 2020 09:14:43: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:14:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:14:43: #1 tags after filtering in treatment: 1602583 INFO @ Tue, 16 Jun 2020 09:14:43: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:14:43: #1 finished! INFO @ Tue, 16 Jun 2020 09:14:43: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:14:43: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:14:43: #2 number of paired peaks: 1070 INFO @ Tue, 16 Jun 2020 09:14:43: start model_add_line... INFO @ Tue, 16 Jun 2020 09:14:43: start X-correlation... INFO @ Tue, 16 Jun 2020 09:14:43: end of X-cor INFO @ Tue, 16 Jun 2020 09:14:43: #2 finished! INFO @ Tue, 16 Jun 2020 09:14:43: #2 predicted fragment length is 145 bps INFO @ Tue, 16 Jun 2020 09:14:43: #2 alternative fragment length(s) may be 50,85,145 bps INFO @ Tue, 16 Jun 2020 09:14:43: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX495091/SRX495091.05_model.r INFO @ Tue, 16 Jun 2020 09:14:43: #3 Call peaks... INFO @ Tue, 16 Jun 2020 09:14:43: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 09:14:47: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 09:14:49: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX495091/SRX495091.05_peaks.xls INFO @ Tue, 16 Jun 2020 09:14:49: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX495091/SRX495091.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 09:14:49: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX495091/SRX495091.05_summits.bed INFO @ Tue, 16 Jun 2020 09:14:49: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (1081 records, 4 fields): 2 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:15:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX495091/SRX495091.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX495091/SRX495091.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX495091/SRX495091.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX495091/SRX495091.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:15:06: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:15:06: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:15:10: 1000000 INFO @ Tue, 16 Jun 2020 09:15:13: #1 tag size is determined as 42 bps INFO @ Tue, 16 Jun 2020 09:15:13: #1 tag size = 42 INFO @ Tue, 16 Jun 2020 09:15:13: #1 total tags in treatment: 1602583 INFO @ Tue, 16 Jun 2020 09:15:13: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:15:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:15:13: #1 tags after filtering in treatment: 1602583 INFO @ Tue, 16 Jun 2020 09:15:13: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:15:13: #1 finished! INFO @ Tue, 16 Jun 2020 09:15:13: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:15:13: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:15:13: #2 number of paired peaks: 1070 INFO @ Tue, 16 Jun 2020 09:15:13: start model_add_line... INFO @ Tue, 16 Jun 2020 09:15:13: start X-correlation... INFO @ Tue, 16 Jun 2020 09:15:13: end of X-cor INFO @ Tue, 16 Jun 2020 09:15:13: #2 finished! INFO @ Tue, 16 Jun 2020 09:15:13: #2 predicted fragment length is 145 bps INFO @ Tue, 16 Jun 2020 09:15:13: #2 alternative fragment length(s) may be 50,85,145 bps INFO @ Tue, 16 Jun 2020 09:15:13: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX495091/SRX495091.10_model.r INFO @ Tue, 16 Jun 2020 09:15:13: #3 Call peaks... INFO @ Tue, 16 Jun 2020 09:15:13: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 09:15:17: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 09:15:19: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX495091/SRX495091.10_peaks.xls INFO @ Tue, 16 Jun 2020 09:15:19: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX495091/SRX495091.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 09:15:19: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX495091/SRX495091.10_summits.bed INFO @ Tue, 16 Jun 2020 09:15:19: Done! pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (480 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 09:15:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX495091/SRX495091.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX495091/SRX495091.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX495091/SRX495091.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX495091/SRX495091.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 09:15:36: #1 read tag files... INFO @ Tue, 16 Jun 2020 09:15:36: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 09:15:40: 1000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 09:15:43: #1 tag size is determined as 42 bps INFO @ Tue, 16 Jun 2020 09:15:43: #1 tag size = 42 INFO @ Tue, 16 Jun 2020 09:15:43: #1 total tags in treatment: 1602583 INFO @ Tue, 16 Jun 2020 09:15:43: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 09:15:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 09:15:43: #1 tags after filtering in treatment: 1602583 INFO @ Tue, 16 Jun 2020 09:15:43: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 09:15:43: #1 finished! INFO @ Tue, 16 Jun 2020 09:15:43: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 09:15:43: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 09:15:43: #2 number of paired peaks: 1070 INFO @ Tue, 16 Jun 2020 09:15:43: start model_add_line... INFO @ Tue, 16 Jun 2020 09:15:44: start X-correlation... INFO @ Tue, 16 Jun 2020 09:15:44: end of X-cor INFO @ Tue, 16 Jun 2020 09:15:44: #2 finished! INFO @ Tue, 16 Jun 2020 09:15:44: #2 predicted fragment length is 145 bps INFO @ Tue, 16 Jun 2020 09:15:44: #2 alternative fragment length(s) may be 50,85,145 bps INFO @ Tue, 16 Jun 2020 09:15:44: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX495091/SRX495091.20_model.r INFO @ Tue, 16 Jun 2020 09:15:44: #3 Call peaks... INFO @ Tue, 16 Jun 2020 09:15:44: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 09:15:48: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 09:15:49: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX495091/SRX495091.20_peaks.xls INFO @ Tue, 16 Jun 2020 09:15:49: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX495091/SRX495091.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 09:15:49: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX495091/SRX495091.20_summits.bed INFO @ Tue, 16 Jun 2020 09:15:49: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (150 records, 4 fields): 1 millis CompletedMACS2peakCalling